acetylcholine [Ligand Id: 294] activity data from GtoPdb and ChEMBL
Click here for a description of the charts and data table
Please tell us if you are using this feature and what you think!
| ChEMBL ligand: CHEMBL667 (Acetylcholine) |
|---|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
| DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
|---|---|---|---|---|---|---|---|---|
| Acetylcholine-binding protein in Lymnaea stagnalis (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6084] [UniProtKB: P58154] | ||||||||
| ChEMBL | Displacement of [3H]-epibatidine from histidine-tagged Lymnaea stagnalis AChBP expressed in Sf9 cells after 1.5 hrs by liquid scintillation counting | B | 5.2 | pKi | 6309.57 | nM | Ki | Bioorg Med Chem (2011) 19: 6107-6119 [PMID:21920761] |
| ChEMBL | Displacement of [125I]alpha-bungarotoxin from Lymnaea stagnalis His-tagged AchBP | B | 5.5 | pKi | 3162.28 | nM | Ki | J Med Chem (2009) 52: 2372-2383 [PMID:19331415] |
| Acetylcholine receptor protein delta chain in Torpedo californica (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3916] [UniProtKB: P02718] | ||||||||
| ChEMBL | Compound was evaluated for functional potencies and efficacies at human Nicotinic acetylcholine receptor subtype TE671 (muscle) | B | 5.3 | pEC50 | 5000 | nM | EC50 | J Med Chem (1997) 40: 4169-4194 [PMID:9435889] |
| M1 receptor/Muscarinic acetylcholine receptor M1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL216] [GtoPdb: 13] [UniProtKB: P11229] | ||||||||
| ChEMBL | Binding affinity to muscarinic M1 acetylcholine receptor (unknown origin) | B | 4.76 | pKd | 17378.01 | nM | Kd | J Med Chem (2015) 58: 560-576 [PMID:25478907] |
| ChEMBL | Displacement of [3H]NMS from human muscarinic M1 acetylcholine receptor expressed in CHO cell membranes by radioligand binding assay | B | 4.76 | pKd | 17378.01 | nM | Kd | J Med Chem (2015) 58: 560-576 [PMID:25478907] |
| ChEMBL | Displacement of [3H]N-methyl-scopolamine from human recombinant muscarinic M1 receptor expressed in CHO-FlpIn cells after 6 hrs by liquid scintillation counting method | B | 4.64 | pKi | 23000 | nM | Ki | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Displacement of [3H]N-methyl-scopolamine from human recombinant muscarinic M1 receptor expressed in CHO-FlpIn cells after 6 hrs by liquid scintillation counting method | B | 4.64 | pKi | 22908.68 | nM | Ki | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Displacement of [3H]NMS from human cloned muscarinic M1 receptor expressed in CHO cells | B | 4.87 | pKi | 13489.63 | nM | Ki | Bioorg Med Chem Lett (2008) 18: 614-618 [PMID:18077164] |
| GtoPdb | - | - | 4.9 | pKi | - | - | - |
Mol Pharmacol (1997) 52: 172-9 [PMID:9224827]; Mol Pharmacol (2013) 84: 425-37 [PMID:23798605] |
| ChEMBL | Binding affinity to muscarinic M1 acetylcholine receptor (unknown origin) | B | 4.9 | pKi | 12589.25 | nM | Ki | J Med Chem (2015) 58: 560-576 [PMID:25478907] |
| ChEMBL | Displacement of [3H]N-methyl-scopolamine bromide from human recombinant muscarinic M1 receptor expressed in HEK293T cell membranes after 1 hr by liquid scintillation counting method | B | 5.24 | pKi | 5800 | nM | Ki | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at human muscarinic M1 receptor expressed in HEK293T cells co-expressing Galpha subunit and PLC-beta3 by split luciferase complementation assay | B | 6.87 | pIC50 | 134.9 | nM | IC50 | J Med Chem (2019) 62: 3009-3020 [PMID:30827105] |
| ChEMBL | Agonist activity at human m1 muscarinic acetylcholine receptor expressed in HEK293 cells assessed as calcium mobilization by FLIPR assay | F | 4 | pEC50 | 100000 | nM | EC50 | Bioorg Med Chem Lett (2015) 25: 1546-1551 [PMID:25765911] |
| ChEMBL | Agonist activity at human muscarinic M1 receptor Y381A mutant expressed in CHO-K1 cells assessed as increase of acetylcholine-induced calcium flux by FLIPR assay | F | 4.57 | pEC50 | 27000 | nM | EC50 | J Med Chem (2010) 53: 6386-6397 [PMID:20684563] |
| ChEMBL | Agonist activity at human muscarinic M1 receptor Y381A mutant expressed in BHK-21 cells assessed as calcium mobilization for 6 mins by Calcium4-based staining | F | 4.57 | pEC50 | 27000 | nM | EC50 | Bioorg Med Chem Lett (2012) 22: 5134-5140 [PMID:22677319] |
| ChEMBL | Agonistic activity against M1 muscarinic receptor expressed in NIH 3T3 cells. | F | 4.7 | pEC50 | 20000 | nM | EC50 | J Med Chem (2001) 44: 4563-4576 [PMID:11741475] |
| ChEMBL | Agonist activity at Gq/11 coupled recombinant human muscarinic M1 AChR expressed in CHO-FlpIn cells assessed as IP-one accumulation after 40 mins by HTRF assay | B | 6.21 | pEC50 | 620 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at Gq/11 coupled recombinant human muscarinic M1 AChR expressed in CHO-FlpIn cells assessed as IP-one accumulation after 40 mins by HTRF assay | B | 6.21 | pEC50 | 616.6 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Partial agonist activity at human muscarinic M1 acetylcholine receptor expressed in CHO cells assessed as increase in IP1 accumulation incubated for 30 mins by FRET based HTRF assay | F | 6.43 | pEC50 | 371.54 | nM | EC50 | J Med Chem (2015) 58: 560-576 [PMID:25478907] |
| ChEMBL | Agonist activity at recombinant human muscarinic M1 receptor expressed in CHO cells assessed as upregulation in ERK1/2 phosphorylation after 5 mins by alphascreen assay | B | 7.33 | pEC50 | 47 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at recombinant human muscarinic M1 receptor expressed in CHO cells assessed as upregulation in ERK1/2 phosphorylation after 5 mins by alphascreen assay | B | 7.33 | pEC50 | 46.77 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at human muscarinic M1 receptor expressed in CHO cells after 30 mins by GTPgamma35S binding assay | F | 7.59 | pEC50 | 25.9 | nM | EC50 | Bioorg Med Chem Lett (2015) 25: 4158-4163 [PMID:26299349] |
| ChEMBL | Agonist activity at human M1 receptor expressed in CHO-K1 cells assessed as calcium mobilization for 6 mins by Calcium4-based staining | F | 8.96 | pEC50 | 1.1 | nM | EC50 | Bioorg Med Chem Lett (2012) 22: 5134-5140 [PMID:22677319] |
| ChEMBL | Agonist activity at human muscarinic M1 receptor expressed in CHO-K1 cells assessed as increase of acetylcholine-induced calcium flux by FLIPR assay | F | 8.96 | pEC50 | 1.1 | nM | EC50 | J Med Chem (2010) 53: 6386-6397 [PMID:20684563] |
| M1 receptor in Rat [GtoPdb: 13] [UniProtKB: P08482] | ||||||||
| GtoPdb | - | - | 5 | pKi | - | - | - | J Pharmacol Exp Ther (2002) 303: 29-35 [PMID:12235229] |
| M2 receptor/Muscarinic acetylcholine receptor M2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL211] [GtoPdb: 14] [UniProtKB: P08172] | ||||||||
| ChEMBL | Displacement of [3H]N-methyl-scopolamine bromide from human recombinant muscarinic M2 receptor expressed in HEK293T cell membranes after 1 hr by liquid scintillation counting method | B | 6.41 | pKi | 390 | nM | Ki | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Displacement of [3H]N-methyl-scopolamine from human recombinant muscarinic M2 receptor expressed in CHO-FlpIn cells after 6 hrs by liquid scintillation counting method | B | 6.42 | pKi | 380.19 | nM | Ki | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Displacement of [3H]N-methyl-scopolamine from human recombinant muscarinic M2 receptor expressed in CHO-FlpIn cells after 6 hrs by liquid scintillation counting method | B | 6.42 | pKi | 380 | nM | Ki | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| GtoPdb | - | - | 6.5 | pKi | - | - | - |
J Pharmacol Exp Ther (2002) 303: 29-35 [PMID:12235229]; Mol Pharmacol (1997) 52: 172-9 [PMID:9224827]; Mol Pharmacol (2013) 84: 425-37 [PMID:23798605] |
| ChEMBL | Displacement of [3H]NMS from human cloned muscarinic M2 receptor expressed in CHO cells | B | 6.8 | pKi | 158.49 | nM | Ki | Bioorg Med Chem Lett (2008) 18: 614-618 [PMID:18077164] |
| ChEMBL | Agonist activity at recombinant human muscarinic M2 receptor expressed in HEK293 cells co-expressing beta-arrestin assessed as increase in beta-arrestin recruitment after 24 hrs by Bright-Glo luminescence assay | B | 5.72 | pEC50 | 1905.46 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at recombinant human muscarinic M2 receptor expressed in HEK293 cells co-expressing beta-arrestin assessed as increase in beta-arrestin recruitment after 24 hrs by Bright-Glo luminescence assay | B | 5.72 | pEC50 | 1900 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at Gi/o protein-coupled recombinant human muscarinic M2 receptor expressed in CHO-FlpIn cells preincubated for 60 mins followed by [35S]GTPgammaS addition measured after 30 mins by liquid scintillation counting method | F | 6.29 | pEC50 | 512.86 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at Gi/o protein-coupled recombinant human muscarinic M2 receptor expressed in CHO-FlpIn cells preincubated for 60 mins followed by [35S]GTPgammaS addition measured after 30 mins by liquid scintillation counting method | F | 6.29 | pEC50 | 510 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at human muscarinic M2 receptor expressed in CHO-K1 cells assessed as calcium mobilization for 6 mins by Calcium4-based staining | F | 6.66 | pEC50 | 220 | nM | EC50 | Bioorg Med Chem Lett (2012) 22: 5134-5140 [PMID:22677319] |
| ChEMBL | Agonist activity at human muscarinic M2 receptor expressed in CHO-K1 cells coexpressing Galpha16 subunit assessed as increase of acetylcholine-induced calcium flux by FLIPR assay | F | 6.66 | pEC50 | 220 | nM | EC50 | J Med Chem (2010) 53: 6386-6397 [PMID:20684563] |
| ChEMBL | Agonist activity at human recombinant muscarinic M2 receptor co-expressed with Galphaqi5-HA in African green monkey COS7 cells assessed as IP accumulation after 2 hrs in presence of myo-[3H]inositol by liquid scintillation counting method | F | 7.25 | pEC50 | 56 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at recombinant human muscarinic M2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-mediated cAMP accumulation after 10 mins by FRET assay | F | 7.31 | pEC50 | 49 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at recombinant human muscarinic M2 receptor expressed in HEK293 cells assessed as inhibition of forskolin-mediated cAMP accumulation after 10 mins by FRET assay | F | 7.31 | pEC50 | 48.98 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at recombinant human muscarinic M2 receptor expressed in CHO cells assessed as upregulation in ERK1/2 phosphorylation after 5 mins by alphascreen assay | B | 7.89 | pEC50 | 13 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at recombinant human muscarinic M2 receptor expressed in CHO cells assessed as upregulation in ERK1/2 phosphorylation after 5 mins by alphascreen assay | B | 7.9 | pEC50 | 12.59 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at human muscarinic M2 receptor expressed in CHO cells after 30 mins by GTPgamma35S binding assay | F | 8.15 | pEC50 | 7 | nM | EC50 | Bioorg Med Chem Lett (2015) 25: 4158-4163 [PMID:26299349] |
| M2 receptor in Rat [GtoPdb: 14] [UniProtKB: P10980] | ||||||||
| GtoPdb | - | - | 6.4 | pKi | - | - | - | J Pharmacol Exp Ther (1998) 284: 500-7 [PMID:9454790] |
| M3 receptor/Muscarinic acetylcholine receptor M3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL245] [GtoPdb: 15] [UniProtKB: P20309] | ||||||||
| ChEMBL | Displacement of [3H]NMS from human cloned muscarinic M3 receptor expressed in CHO cells | B | 5.15 | pKi | 7079.46 | nM | Ki | Bioorg Med Chem Lett (2008) 18: 614-618 [PMID:18077164] |
| ChEMBL | Displacement of [3H]N-methyl-scopolamine from human recombinant muscarinic M3 receptor expressed in CHO-FlpIn cells after 6 hrs by liquid scintillation counting method | B | 5.17 | pKi | 6800 | nM | Ki | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Displacement of [3H]N-methyl-scopolamine from human recombinant muscarinic M3 receptor expressed in CHO-FlpIn cells after 6 hrs by liquid scintillation counting method | B | 5.17 | pKi | 6760.83 | nM | Ki | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Displacement of [3H]N-methyl-scopolamine bromide from human recombinant muscarinic M3 receptor expressed in HEK293T cell membranes after 1 hr by liquid scintillation counting method | B | 5.33 | pKi | 4700 | nM | Ki | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| GtoPdb | - | - | 5.4 | pKi | - | - | - |
J Pharmacol Exp Ther (2002) 303: 29-35 [PMID:12235229]; Mol Pharmacol (1997) 52: 172-9 [PMID:9224827]; Mol Pharmacol (2013) 84: 425-37 [PMID:23798605] |
| ChEMBL | Agonist activity at Gq/11 coupled recombinant human muscarinic M3 AChR expressed in CHO-FlpIn cells assessed as IP-one accumulation after 40 mins by HTRF assay | B | 6.62 | pEC50 | 240 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at Gq/11 coupled recombinant human muscarinic M3 AChR expressed in CHO-FlpIn cells assessed as IP-one accumulation after 40 mins by HTRF assay | B | 6.62 | pEC50 | 239.88 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at human muscarinic M3 receptor expressed in CHO cells after 30 mins by GTPgamma35S binding assay | F | 6.69 | pEC50 | 205 | nM | EC50 | Bioorg Med Chem Lett (2015) 25: 4158-4163 [PMID:26299349] |
| ChEMBL | Agonist activity at recombinant human muscarinic M3 receptor expressed in CHO cells assessed as upregulation in ERK1/2 phosphorylation after 5 mins by alphascreen assay | B | 7.64 | pEC50 | 23 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at recombinant human muscarinic M3 receptor expressed in CHO cells assessed as upregulation in ERK1/2 phosphorylation after 5 mins by alphascreen assay | B | 7.64 | pEC50 | 22.91 | nM | EC50 | J Med Chem (2017) 60: 9239-9250 [PMID:29094937] |
| ChEMBL | Agonist activity at human muscarinic M3 receptor expressed in BHK-21 cells assessed as increase of acetylcholine-induced calcium flux by FLIPR assay | F | 8.49 | pEC50 | 3.2 | nM | EC50 | J Med Chem (2010) 53: 6386-6397 [PMID:20684563] |
| ChEMBL | Agonist activity at human muscarinic M3 receptor expressed in BHK-21 cells assessed as calcium mobilization for 6 mins by Calcium4-based staining | F | 8.49 | pEC50 | 3.2 | nM | EC50 | Bioorg Med Chem Lett (2012) 22: 5134-5140 [PMID:22677319] |
| M3 receptor in Rat [GtoPdb: 15] [UniProtKB: P08483] | ||||||||
| GtoPdb | - | - | 5.6 | pKi | - | - | - | J Pharmacol Exp Ther (2002) 303: 29-35 [PMID:12235229] |
| M4 receptor/Muscarinic acetylcholine receptor M4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1821] [GtoPdb: 16] [UniProtKB: P08173] | ||||||||
| ChEMBL | Displacement of [3H]NMS from human cloned muscarinic M4 receptor expressed in CHO cells | B | 5.07 | pKi | 8511.38 | nM | Ki | Bioorg Med Chem Lett (2008) 18: 614-618 [PMID:18077164] |
| GtoPdb | - | - | 5.6 | pKi | - | - | - |
Mol Pharmacol (1997) 52: 172-9 [PMID:9224827]; Mol Pharmacol (2013) 84: 425-37 [PMID:23798605] |
| ChEMBL | Positive allosteric modulatory activity at human Muscarinic acetylcholine receptor M4 expressed in HEK cells co-expressing Glosensor construct assessed as as increase in acteylcholine-induced cAMP accumulation incubated for 7 mins in presence of isoproterenol/acetylcholine by Glosensor cAMP reagent/plate reader based luminescence assay | F | 7.24 | pEC50 | 57 | nM | EC50 | ACS Med Chem Lett (2019) 10: 941-948 [PMID:31223452] |
| ChEMBL | Agonist activity at human muscarinic M4 receptor expressed in CHO cells after 30 mins by GTPgamma35S binding assay | F | 7.31 | pEC50 | 48.8 | nM | EC50 | Bioorg Med Chem Lett (2015) 25: 4158-4163 [PMID:26299349] |
| ChEMBL | Agonist activity at human Muscarinic acetylcholine receptor M4 expressed in HEK cells co-expressing Glosensor construct assessed as decrease in isoproterenol-induced cAMP accumulation incubated for 7 mins in presence of isoproterenol by Glosensor cAMP reagent/plate reader based luminescence assay | F | 7.37 | pEC50 | 43 | nM | EC50 | ACS Med Chem Lett (2019) 10: 941-948 [PMID:31223452] |
| ChEMBL | Agonist activity at human muscarinic M4 receptor expressed in BHK-21 cells coexpressing Galpha16 subunit assessed as increase of acetylcholine-induced calcium flux by FLIPR assay | F | 8 | pEC50 | 10 | nM | EC50 | J Med Chem (2010) 53: 6386-6397 [PMID:20684563] |
| ChEMBL | Agonist activity at muscarinic human M4 receptor expressed in BHK-21 cells assessed as calcium mobilization for 6 mins by Calcium4-based staining | F | 8 | pEC50 | 10 | nM | EC50 | Bioorg Med Chem Lett (2012) 22: 5134-5140 [PMID:22677319] |
| M5 receptor/Muscarinic acetylcholine receptor M5 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2035] [GtoPdb: 17] [UniProtKB: P08912] | ||||||||
| ChEMBL | Displacement of [3H]NMS from human cloned muscarinic M5 receptor expressed in CHO cells | B | 5.01 | pKi | 9772.37 | nM | Ki | Bioorg Med Chem Lett (2008) 18: 614-618 [PMID:18077164] |
| GtoPdb | - | - | 6.1 | pKi | - | - | - | J Pharmacol Exp Ther (2002) 303: 29-35 [PMID:12235229] |
| ChEMBL | Agonistic activity against muscarinic acetylcholine receptor M5 expressed in NIH 3T3 cells. | F | 6.2 | pEC50 | 630 | nM | EC50 | J Med Chem (2001) 44: 4563-4576 [PMID:11741475] |
| ChEMBL | Agonist activity at human muscarinic M5 receptor expressed in CHO cells after 30 mins by GTPgamma35S binding assay | F | 7.72 | pEC50 | 19.1 | nM | EC50 | Bioorg Med Chem Lett (2015) 25: 4158-4163 [PMID:26299349] |
| ChEMBL | Agonist activity at human muscarinic M5 receptor expressed in BHK-21 cells assessed as increase of acetylcholine-induced calcium flux by FLIPR assay | F | 8.92 | pEC50 | 1.2 | nM | EC50 | J Med Chem (2010) 53: 6386-6397 [PMID:20684563] |
| ChEMBL | Agonist activity at human muscarinic M5 receptor expressed in BHK-21 cells assessed as calcium mobilization for 6 mins by Calcium4-based staining | F | 8.92 | pEC50 | 1.2 | nM | EC50 | Bioorg Med Chem Lett (2012) 22: 5134-5140 [PMID:22677319] |
| nicotinic acetylcholine receptor α3 subunit/nicotinic acetylcholine receptor α4 subunit/nicotinic acetylcholine receptor α2 subunit/nicotinic acetylcholine receptor β2 subunit/nicotinic acetylcholine receptor β3 subunit/nicotinic acetylcholine receptor β4 subunit/nicotinic acetylcholine receptor α5 subunit/nicotinic acetylcholine receptor α6 subunit/nicotinic acetylcholine receptor α9 subunit/nicotinic acetylcholine receptor α7 subunit/nicotinic acetylcholine receptor α10 subunit/Neuronal acetylcholine receptor in Rat (target type: PROTEIN COMPLEX GROUP) [ChEMBL: CHEMBL2094110] [GtoPdb: 464, 465, 463, 472, 473, 474, 466, 467, 469, 468, 470] [UniProtKB: P04757, P09483, P12389, P12390, P12391, P12392, P20420, P43143, P43144, Q05941, Q9JLB5] | ||||||||
| ChEMBL | Binding affinity towards rat forebrain nicotinic acetylcholine receptor using [3H]EB as radioligand | B | 7.37 | pKi | 43 | nM | Ki | Bioorg Med Chem Lett (2004) 14: 1845-1848 [PMID:15050613] |
| ChEMBL | Compound was evaluated for functional potencies and efficacies at rat Nicotinic acetylcholine receptor subtype PC12 (ganglionic) | B | 4.4 | pEC50 | 40000 | nM | EC50 | J Med Chem (1997) 40: 4169-4194 [PMID:9435889] |
| nicotinic acetylcholine receptor α2 subunit/nicotinic acetylcholine receptor β2 subunit/Neuronal acetylcholine receptor; alpha2/beta2 in Rat (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907592] [GtoPdb: 463, 472] [UniProtKB: P12389, P12390] | ||||||||
| ChEMBL | Binding affinity towards rat nicotinic acetylcholine receptor alpha2-beta2 expressed in HEK293 cells using [3H]EB as radioligand | B | 7.96 | pKi | 11 | nM | Ki | Bioorg Med Chem Lett (2004) 14: 1845-1848 [PMID:15050613] |
| nicotinic acetylcholine receptor α3 subunit/nicotinic acetylcholine receptor β2 subunit/Neuronal acetylcholine receptor; alpha3/beta2 in Rat (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907587] [GtoPdb: 464, 472] [UniProtKB: P04757, P12390] | ||||||||
| ChEMBL | Binding affinity towards rat Nicotinic acetylcholine receptor alpha3-beta2 expressed in HEK293 cells using [3H]EB as radioligand | B | 7.39 | pKi | 41 | nM | Ki | Bioorg Med Chem Lett (2004) 14: 1845-1848 [PMID:15050613] |
| nicotinic acetylcholine receptor β4 subunit/nicotinic acetylcholine receptor α3 subunit/Neuronal acetylcholine receptor; alpha3/beta4 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907594] [GtoPdb: 474, 464] [UniProtKB: P30926, P32297] | ||||||||
| ChEMBL | Compound was evaluated for functional potencies and efficacies at human Nicotinic acetylcholine receptor subtype IMR-32 (ganglionic) | B | 4.52 | pEC50 | 30000 | nM | EC50 | J Med Chem (1997) 40: 4169-4194 [PMID:9435889] |
| ChEMBL | Agonist activity at alpha3beta4 nAChR expressed in HEK293 cells by FLIPR membrane potential assay | F | 4.7 | pEC50 | 19952.62 | nM | EC50 | Bioorg Med Chem Lett (2009) 19: 87-91 [PMID:19027295] |
| ChEMBL | Agonist activity at alpha3beta4 nAChR expressed in HEK293 cells by FLIPR membrane potential assay | F | 4.72 | pEC50 | 19000 | nM | EC50 | Bioorg Med Chem Lett (2009) 19: 87-91 [PMID:19027295] |
| nicotinic acetylcholine receptor α3 subunit/nicotinic acetylcholine receptor β4 subunit/Neuronal acetylcholine receptor; alpha3/beta4 in Rat (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907593] [GtoPdb: 464, 474] [UniProtKB: P04757, P12392] | ||||||||
| ChEMBL | Displacement of [3H]epibatidine from Alpha3 Beta4 Nicotinic acetylcholine receptor of rat brain homogenates | B | 6.06 | pKi | 881 | nM | Ki | J Med Chem (2005) 48: 5123-5130 [PMID:16078832] |
| ChEMBL | Binding affinity towards rat Nicotinic acetylcholine receptor alpha3-beta4 expressed in HEK293 cells using [3H]EB as radioligand | B | 6.09 | pKi | 820 | nM | Ki | Bioorg Med Chem Lett (2004) 14: 1845-1848 [PMID:15050613] |
| ChEMBL | Displacement of [3H]epibatidine from rat alpha3beta4 nACHR expressed in HEK293 cells after 4 hrs by scintillation counting assay | B | 6.21 | pKi | 620 | nM | Ki | Eur J Med Chem (2015) 102: 425-444 [PMID:26301559] |
| ChEMBL | Displacement of [3H]epibatidine from rat alpha3beta4 nAChR expressed in HEK292 cells after 3 hrs | B | 6.21 | pKi | 620 | nM | Ki | J Med Chem (2010) 53: 8187-8191 [PMID:20979364] |
| ChEMBL | Displacement of [3H]epibatidine from rat recombinant alpha3beta4 nAChR expressed in HEK293 cells after 4 hrs by scintillation counting analysis | B | 6.21 | pKi | 620 | nM | Ki | J Med Chem (2013) 56: 940-951 [PMID:23256554] |
| ChEMBL | Displacement of [3H]epibatidine from rat recombinant alpha3beta4 nAChR expressed in HEK293 cells after 4 hrs by scintillation counting analysis | B | 6.21 | pKi | 616.6 | nM | Ki | J Med Chem (2013) 56: 940-951 [PMID:23256554] |
| ChEMBL | Displacement of [3H]epibatidine from rat alpha3beta4 nACHR expressed in HEK293 cells after 4 hrs by scintillation counting assay | B | 6.21 | pKi | 616.6 | nM | Ki | Eur J Med Chem (2015) 102: 425-444 [PMID:26301559] |
| ChEMBL | Agonist activity at rat alpha-3-beta-4 nAChR expressed in HEK293 cells by FMP assay | F | 5.05 | pEC50 | 8900 | nM | EC50 | J Med Chem (2008) 51: 7380-7395 [PMID:18989912] |
| ChEMBL | Antagonist activity at rat alpha3beta4 nAChR expressed in HEK293 cells by FMP assay | F | 5.06 | pEC50 | 8700 | nM | EC50 | J Med Chem (2007) 50: 4616-4629 [PMID:17722904] |
| ChEMBL | Antagonist activity at rat alpha3beta4 nAChR expressed in HEK293 cells by FMP assay | F | 5.1 | pEC50 | 7943.28 | nM | EC50 | J Med Chem (2007) 50: 4616-4629 [PMID:17722904] |
| ChEMBL | Agonist activity at rat alpha-3-beta-4 nAChR expressed in HEK293 cells by FMP assay | F | 5.1 | pEC50 | 7943.28 | nM | EC50 | J Med Chem (2008) 51: 7380-7395 [PMID:18989912] |
| nicotinic acetylcholine receptor β2 subunit/nicotinic acetylcholine receptor α4 subunit/Neuronal acetylcholine receptor; alpha4/beta2 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907589] [GtoPdb: 472, 465] [UniProtKB: P17787, P43681] | ||||||||
| ChEMBL | Agonist activity at human alpha4beta2 nAChR in (alpha4)3(beta2)2 stoichiometry form | B | 4 | pEC50 | 100000 | nM | EC50 | J Med Chem (2015) 58: 6665-6677 [PMID:26225816] |
| ChEMBL | Agonist activity at human alpha4beta2 nAChR low affinity form expressed in rat GH4C1 cells assessed as induction of peak current amplitude at holding potential of -70 mV by whole-cell patch clamp assay | B | 4 | pEC50 | 100000 | nM | EC50 | J Med Chem (2015) 58: 6665-6677 [PMID:26225816] |
| ChEMBL | Agonist activity at alpha4beta2 nAChR low sensitivity form | F | 4 | pEC50 | 100000 | nM | EC50 | J Med Chem (2012) 55: 9929-9945 [PMID:23025891] |
| ChEMBL | Agonist activity at human alpha-4-beta-2 nAChR expressed in Xenopus oocytes by two-electrode voltage clamp technique | F | 4.15 | pEC50 | 70794.58 | nM | EC50 | J Med Chem (2008) 51: 7380-7395 [PMID:18989912] |
| ChEMBL | Agonist activity at human alpha-4-beta-2 nAChR expressed in Xenopus oocytes by two-electrode voltage clamp technique | F | 4.15 | pEC50 | 70000 | nM | EC50 | J Med Chem (2008) 51: 7380-7395 [PMID:18989912] |
| ChEMBL | Partial agonist activity at human 2:3 alpha4beta2 nAChR expressed in Xenopus laevis oocytes after 1 min by two electrode voltage clamp technique | B | 5.8 | pEC50 | 1600 | nM | EC50 | J Med Chem (2013) 56: 940-951 [PMID:23256554] |
| ChEMBL | Agonist activity at human alpha4beta2 nAChR in (alpha4)2(beta2)3 stoichiometry form | B | 6 | pEC50 | 1000 | nM | EC50 | J Med Chem (2015) 58: 6665-6677 [PMID:26225816] |
| ChEMBL | Agonist activity at alpha4beta2 nAChR high sensitivity form | F | 6 | pEC50 | 1000 | nM | EC50 | J Med Chem (2012) 55: 9929-9945 [PMID:23025891] |
| ChEMBL | Agonist activity at human alpha4beta2 nAChR high affinity form expressed in rat GH4C1 cells assessed as induction of peak current amplitude at holding potential of -70 mV by whole-cell patch clamp assay | B | 6.4 | pEC50 | 400 | nM | EC50 | J Med Chem (2015) 58: 6665-6677 [PMID:26225816] |
| ChEMBL | Activation of alpha4beta2* nAChR low affinity site (unknown origin) expressed in xenopus oocytes by electrophysiology method | B | 7.14 | pEC50 | 73 | nM | EC50 | J Med Chem (2014) 57: 8204-8223 [PMID:24901260] |
| ChEMBL | Activation of alpha4beta2* nAChR high affinity site (unknown origin) expressed in xenopus oocytes by electrophysiology method | B | 8.77 | pEC50 | 1.7 | nM | EC50 | J Med Chem (2014) 57: 8204-8223 [PMID:24901260] |
| nicotinic acetylcholine receptor α4 subunit/nicotinic acetylcholine receptor β2 subunit/Neuronal acetylcholine receptor; alpha4/beta2 in Rat (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907596] [GtoPdb: 465, 472] [UniProtKB: P09483, P12390] | ||||||||
| ChEMBL | Binding affinity towards rat Nicotinic acetylcholine receptor alpha4-beta2 expressed in HEK293 cells using [3H]EB as radioligand | B | 7.35 | pKi | 45 | nM | Ki | Bioorg Med Chem Lett (2004) 14: 1845-1848 [PMID:15050613] |
| ChEMBL | Displacement of [3H]cystisine from nicotinic alpha-4-beta-2 receptor in rat brain | B | 7.41 | pKi | 39 | nM | Ki | Bioorg Med Chem Lett (2008) 18: 2188-2193 [PMID:18262785] |
| ChEMBL | Displacement of [3H]epibatidine from rat alpha4beta2 nAChR expressed in HEK292 cells after 3 hrs | B | 7.42 | pKi | 37.7 | nM | Ki | J Med Chem (2010) 53: 8187-8191 [PMID:20979364] |
| ChEMBL | Displacement of [3H]epibatidine from rat alpha4beta2 nAChR expressed in HEK293 cells after 4 hrs by scintillation counting assay | B | 7.48 | pKi | 33.11 | nM | Ki | Eur J Med Chem (2015) 102: 425-444 [PMID:26301559] |
| ChEMBL | Displacement of [3H]epibatidine from rat recombinant alpha4beta2 nAChR expressed in HEK293 cells after 4 hrs by scintillation counting analysis | B | 7.48 | pKi | 33.11 | nM | Ki | J Med Chem (2013) 56: 940-951 [PMID:23256554] |
| ChEMBL | Displacement of [3H]epibatidine from rat recombinant alpha4beta2 nAChR expressed in HEK293 cells after 4 hrs by scintillation counting analysis | B | 7.48 | pKi | 33 | nM | Ki | J Med Chem (2013) 56: 940-951 [PMID:23256554] |
| ChEMBL | Displacement of [3H]epibatidine from rat alpha4beta2 nAChR expressed in HEK293 cells after 4 hrs by scintillation counting assay | B | 7.48 | pKi | 33 | nM | Ki | Eur J Med Chem (2015) 102: 425-444 [PMID:26301559] |
| ChEMBL | Displacement of [3H]-cytisine from Alpha4-beta2 Nicotinic acetylcholine receptor of rat brain homogenates | B | 8.12 | pKi | 7.6 | nM | Ki | J Med Chem (2005) 48: 5123-5130 [PMID:16078832] |
| ChEMBL | Functional potency measured as stimulation at rat Nicotinic acetylcholine receptor alpha4-beta2 expressed in Xenopus oocytes | F | 4.06 | pEC50 | 87000 | nM | EC50 | Bioorg Med Chem Lett (2004) 14: 1889-1896 [PMID:15050621] |
| ChEMBL | Agonist activity at rat alpha4beta2 expressed in Xenopus oocytes at -60 mV holding potential by electrophysiology method | B | 4.62 | pEC50 | 24000 | nM | EC50 | J Nat Prod (2018) 81: 1029-1035 [PMID:29671588] |
| nicotinic acetylcholine receptor α4 subunit/nicotinic acetylcholine receptor β4 subunit/Neuronal acetylcholine receptor; alpha4/beta4 in Rat (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907595] [GtoPdb: 465, 474] [UniProtKB: P09483, P12392] | ||||||||
| ChEMBL | Binding affinity towards rat Nicotinic acetylcholine receptor alpha4-beta4 expressed in HEK293 cells using [3H]EB as radioligand | B | 7.08 | pKi | 83 | nM | Ki | Bioorg Med Chem Lett (2004) 14: 1845-1848 [PMID:15050613] |
| ChEMBL | Displacement of [3H]epibatidine from rat recombinant alpha4beta4 nAChR expressed in HEK293 cells after 4 hrs by scintillation counting analysis | B | 7.24 | pKi | 58 | nM | Ki | J Med Chem (2013) 56: 940-951 [PMID:23256554] |
| ChEMBL | Displacement of [3H]epibatidine from rat alpha4beta4 nACHR expressed in HEK293 cells after 4 hrs by scintillation counting assay | B | 7.24 | pKi | 58 | nM | Ki | Eur J Med Chem (2015) 102: 425-444 [PMID:26301559] |
| ChEMBL | Displacement of [3H]epibatidine from rat recombinant alpha4beta4 nAChR expressed in HEK293 cells after 4 hrs by scintillation counting analysis | B | 7.24 | pKi | 57.54 | nM | Ki | J Med Chem (2013) 56: 940-951 [PMID:23256554] |
| ChEMBL | Displacement of [3H]epibatidine from rat alpha4beta4 nACHR expressed in HEK293 cells after 4 hrs by scintillation counting assay | B | 7.24 | pKi | 57.54 | nM | Ki | Eur J Med Chem (2015) 102: 425-444 [PMID:26301559] |
| ChEMBL | Functional potency measured as stimulation at rat Nicotinic acetylcholine receptor alpha4-beta4 expressed in Xenopus oocytes | F | 4.72 | pEC50 | 19000 | nM | EC50 | Bioorg Med Chem Lett (2004) 14: 1889-1896 [PMID:15050621] |
| nicotinic acetylcholine receptor α7 subunit/Neuronal acetylcholine receptor protein alpha-7 subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2492] [GtoPdb: 468] [UniProtKB: P36544] | ||||||||
| ChEMBL | Displacement of [3H]-MLA from human alpha7 nAChR expressed in human SH-SY5Y cells after 1.5 hrs by liquid scintillation counting | B | 5.1 | pKi | 7943.28 | nM | Ki | Bioorg Med Chem (2011) 19: 6107-6119 [PMID:21920761] |
| ChEMBL | Displacement of [3H]epibatidine from chimeric human alpha7-5-HT3A receptor expressed in HEK293 cells after 2 hrs by scintillation counter analysis | B | 6.3 | pKi | 501.19 | nM | Ki | Bioorg Med Chem (2012) 20: 5992-6002 [PMID:22959526] |
| ChEMBL | Agonist activity at human recombinant alpha7 nAChR expressed in Xenopus oocyte | F | 4.06 | pEC50 | 87000 | nM | EC50 | ACS Med Chem Lett (2010) 1: 422-426 [PMID:24900227] |
| ChEMBL | Agonist activity at human alpha-7 nAChR expressed in Xenopus oocytes by two-electrode voltage clamp technique | F | 4.08 | pEC50 | 83176.38 | nM | EC50 | J Med Chem (2008) 51: 7380-7395 [PMID:18989912] |
| ChEMBL | Agonist activity at human alpha-7 nAChR expressed in Xenopus oocytes by two-electrode voltage clamp technique | F | 4.08 | pEC50 | 83000 | nM | EC50 | J Med Chem (2008) 51: 7380-7395 [PMID:18989912] |
| ChEMBL | Agonist activity at human recombinant alpha7 nAChR expressed in Xenopus oocyte in presence of 6 uM (S)-ethyl 1-(2-cyanoethyl)pyrrolidine-2-carboxylate | F | 4.16 | pEC50 | 69000 | nM | EC50 | ACS Med Chem Lett (2010) 1: 422-426 [PMID:24900227] |
| ChEMBL | Antagonist activity at human alpha7nAChR co-expressed with human Ric3 in tsA201 cells by FMP assay | F | 5.4 | pEC50 | 4000 | nM | EC50 | J Med Chem (2007) 50: 4616-4629 [PMID:17722904] |
| ChEMBL | Antagonist activity at human alpha-7 nAChR in tsA201 cells coexpressed with Ric3 by FMP assay | F | 5.4 | pEC50 | 4000 | nM | EC50 | J Med Chem (2007) 50: 4616-4629 [PMID:17722904] |
| ChEMBL | Antagonist activity at human alpha7nAChR co-expressed with human Ric3 in tsA201 cells by FMP assay | F | 5.4 | pEC50 | 3981.07 | nM | EC50 | J Med Chem (2007) 50: 4616-4629 [PMID:17722904] |
| ChEMBL | Antagonist activity at human alpha-7 nAChR in tsA201 cells coexpressed with Ric3 by FMP assay | F | 5.4 | pEC50 | 3981.07 | nM | EC50 | J Med Chem (2007) 50: 4616-4629 [PMID:17722904] |
| nicotinic acetylcholine receptor α7 subunit/Neuronal acetylcholine receptor protein alpha-7 subunit in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4980] [GtoPdb: 468] [UniProtKB: Q05941] | ||||||||
| ChEMBL | Displacement of [125I]-alpha-bungarotoxin from alpha7 nAChR in rat hippocampus membranes | B | 4.6 | pKi | 25000 | nM | Ki | Bioorg Med Chem Lett (2013) 23: 6481-6485 [PMID:24128660] |
| ChEMBL | Displacement of [3H]alpha-bungaro-toxine from nicotinic alpha-7 receptor in rat brain | B | 5.45 | pKi | 3509 | nM | Ki | Bioorg Med Chem Lett (2008) 18: 2188-2193 [PMID:18262785] |
| ChEMBL | Agonist activity at rat alpha7 nAChR expressed in GH4C1 cells assessed as calcium influx measured every 1 sec for 1 min followed by every 30 secs for 10 mins by FLIPR assay | F | 5.51 | pEC50 | 3120 | nM | EC50 | J Med Chem (2012) 55: 4806-4823 [PMID:22468936] |
| Nicotinic acetylcholine receptor alpha8 subunit in Periplaneta americana (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2366437] [UniProtKB: H6TY12] | ||||||||
| ChEMBL | Agonist activity at Periplaneta americana (American cockroach) nAChR in terminal abdominal ganglion assessed as assessed as inward current by tight-seal whole cell patch clamp method | B | 4.94 | pEC50 | 11481.54 | nM | EC50 | J Pestic Sci (2006) 31: 35-40 |
ChEMBL data shown on this page come from version 33:
Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]
