ChEMBL ligand: CHEMBL714 (Accuneb, Aerolin, Aerolin 400, Aerolin auto, Airomir, Airsalb, Albuterol, Asmasal clickhaler, Asmasal spacehaler, Asmaven, Asmavent, Cobutolin, Combivent, Combivent udvs, Duoneb, Etinoline, Ipramol steri-neb, Kentamol, Libetist, Maxivent, NSC-757417, Proair Hfa, Proventil, Proventil Hfa, PROVENTIL-HFA, Rimasal, Salamol e-breathe, Salapin, Salbulin novolizer, Salbutamol, Salbutamolum, Salbuvent, Salipraneb, SCH 13949W, SCH-13949W, Steri-neb salamol, Ventmax sr, Ventodisks, Ventolin, Ventolin cr, Ventoline, Ventolin e-breathe, Ventolin Hfa, Volmax)
β1-adrenoceptor/Beta-1 adrenergic receptor in Human [ChEMBL: CHEMBL213] [GtoPdb: 28] [UniProtKB: P08588] There should be some charts here, you may need to enable JavaScript!
β2-adrenoceptor/Beta-2 adrenergic receptor in Human [ChEMBL: CHEMBL210] [GtoPdb: 29] [UniProtKB: P07550] Beta-2 adrenergic receptor in Bovine [ChEMBL: CHEMBL3373] [UniProtKB: Q28044] Beta-2 adrenergic receptor in Guinea pig [ChEMBL: CHEMBL5414] [UniProtKB: Q8K4Z4] There should be some charts here, you may need to enable JavaScript!
GroEL/GroES in Escherichia coli [ChEMBL: CHEMBL4106139] [UniProtKB: Q548M1, Q7BGE6] There should be some charts here, you may need to enable JavaScript!
HSP60/HSP10 in Human [ChEMBL: CHEMBL4106131] [UniProtKB: P10809, P61604] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
β1-adrenoceptor/Beta-1 adrenergic receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL213] [GtoPdb: 28] [UniProtKB: P08588]
ChEMBL	Agonist activity at human beta1 adrenoceptor expressed in CHO cells assessed as increase in intracellular cAMP level incubated for 1 hr by alphascreen technology	F	5.93	pEC50	1170	nM	EC50	J Med Chem (2020) 63: 15218-15242 [PMID:33213146]
β2-adrenoceptor/Beta-2 adrenergic receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL210] [GtoPdb: 29] [UniProtKB: P07550]
ChEMBL	Displacement of [3H]DHA from inactive/G protein-uncoupled human beta2-AR expressed in CHO cell membranes assessed as intrinsic Kd by liquid scintillation counting	B	5.85	pKd	1400	nM	Kd	J Med Chem (2016) 59: 5780-5789 [PMID:27239696]
ChEMBL	Displacement of [3H]DHA from inactive/G protein-uncoupled human beta2-AR expressed in CHO cell membranes by liquid scintillation counting	B	6.66	pKd	220	nM	Kd	J Med Chem (2016) 59: 5780-5789 [PMID:27239696]
ChEMBL	Displacement of [125I]cyanopindolol from human recombinant beta2 adrenergic receptor expressed in CHO cells by filtration assay	B	5.74	pKi	1828	nM	Ki	J Med Chem (2010) 53: 3675-3684 [PMID:20402514]
GtoPdb	-	-	6.1	pKi	-	-	-	Mol Pharmacol (1999) 56: 875-85 [PMID:10531390]; Br J Pharmacol (2005) 144: 317-22 [PMID:15655528]; Br J Pharmacol (2010) 160: 1048-61 [PMID:20590599]; Bioorg Med Chem (2012) 20: 933-41 [PMID:22182578]; Br J Pharmacol (2022) 179: 4692-4708 [PMID:35732075]; Mol Pharmacol (2015) 87: 103-20 [PMID:25324048]
ChEMBL	DRUGMATRIX: Adrenergic beta2 radioligand binding (ligand: [3H] CGP-12177)	B	6.25	pKi	557	nM	Ki	DrugMatrix in vitro pharmacology data
ChEMBL	Displacement of [3H]CGP1217 from human recombinant adrenergic beta2 receptor transfected in insect Sf9 cells by scintillation counting	B	6.29	pKi	510	nM	Ki	J Med Chem (2009) 52: 1773-1777 [PMID:19245211]
ChEMBL	Displacement of [3H]dihydroalprenolol from beta2-adrenoceptor after 90 mins by liquid scintillation counting	B	5.7	pIC50	1995.26	nM	IC50	Bioorg Med Chem Lett (2012) 22: 1213-1218 [PMID:22178551]
ChEMBL	Displacement of [3H]CGP1217 from human recombinant adrenergic beta2 receptor transfected in insect Sf9 cells by scintillation counting	B	6.01	pIC50	980	nM	IC50	J Med Chem (2009) 52: 1773-1777 [PMID:19245211]
ChEMBL	DRUGMATRIX: Adrenergic beta2 radioligand binding (ligand: [3H] CGP-12177)	B	6.09	pIC50	810.2	nM	IC50	DrugMatrix in vitro pharmacology data
ChEMBL	Agonist activity at human beta2 adrenoceptor expressed in CHO cells assessed as increase in intracellular cAMP level incubated for 1 hr by alphascreen technology	F	6.6	pEC50	251	nM	EC50	J Med Chem (2020) 63: 15218-15242 [PMID:33213146]
ChEMBL	Agonist activity at human adrenergic beta2 receptor expressed in H292 cells assessed as intracellular cAMP accumulation after 1 hr	F	6.7	pEC50	199.53	nM	EC50	Bioorg Med Chem Lett (2011) 21: 4027-4031 [PMID:21652207]
ChEMBL	Agonist activity at human beta2 adrenergic receptor assessed as increase in cAMP level by whole cell assay	F	7.17	pEC50	68	nM	EC50	J Med Chem (2010) 53: 3675-3684 [PMID:20402514]
ChEMBL	Agonist activity at recombinant human beta2-adrenoceptor expressed in whole cells assessed as cAMP accumulation by homogeneous radioimmunoassay	F	7.6	pEC50	25.12	nM	EC50	Bioorg Med Chem Lett (2012) 22: 1213-1218 [PMID:22178551]
GtoPdb	-	-	7.7	pEC50	-	-	-	Br J Pharmacol (2010) 160: 1048-61 [PMID:20590599]; Br J Pharmacol (2022) 179: 4692-4708 [PMID:35732075]
Beta-2 adrenergic receptor in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3373] [UniProtKB: Q28044]
ChEMBL	Apparent binding affinity constant to beta-2 adrenergic receptor determined using [3H]DHA	B	6.24	pKd	0.57	uM	KD app	J Med Chem (1986) 29: 549-554 [PMID:2870189]
ChEMBL	Affinity for cow beta-2 adrenergic receptor by measuring displacement (-)-[3H]dihydroalprenolol (DHA)	B	6.28	pKd	520	nM	Kd	J Med Chem (1985) 28: 1328-1334 [PMID:2993621]
ChEMBL	Affinity for cow beta-2 adrenergic receptor by measuring displacement (-)-[3H]dihydroalprenolol (DHA)	B	6.58	pKd	6.58	-	-Log KD	J Med Chem (1985) 28: 1328-1334 [PMID:2993621]
Beta-2 adrenergic receptor in Guinea pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5414] [UniProtKB: Q8K4Z4]
ChEMBL	Binding affinity to adrenergic beta2 receptor in Hartley guinea pig tracheal ring	B	5.94	pKd	1160	nM	Kd	Eur J Med Chem (2009) 44: 2840-2846 [PMID:19168263]
ChEMBL	Agonist activity at adrenergic beta2 receptor in Hartley guinea pig tracheal ring assessed as bronchorelaxation activity against histamine-induced contraction	B	6.61	pKd	245.47	nM	Kd	Bioorg Med Chem Lett (2015) 25: 820-825 [PMID:25592716]
ChEMBL	Agonist activity at adrenergic beta2 receptor in Hartley guinea pig tracheal ring at basal tone assessed as bronchorelaxation activity	B	6.96	pKd	109.65	nM	Kd	Bioorg Med Chem Lett (2015) 25: 820-825 [PMID:25592716]
ChEMBL	Agonist activity at beta2 adrenergic receptor in guinea pig tracheal strip assessed as inhibition of electrically-induced bronchocontractile response after 30 mins	F	7.77	pIC50	17	nM	IC50	J Med Chem (2010) 53: 3675-3684 [PMID:20402514]
ChEMBL	Agonist activity at adrenergic beta2 receptor in Hartley guinea pig tracheal ring assessed as bronchorelaxation activity against carbachol-induced contraction	F	6.57	pEC50	268	nM	EC50	Eur J Med Chem (2009) 44: 2840-2846 [PMID:19168263]
ChEMBL	Agonist activity at adrenergic beta2 receptor in guinea pig trachea assessed as relaxation of carbachol-induced tissue contraction	F	8.04	pEC50	9.1	nM	EC50	J Med Chem (2009) 52: 1773-1777 [PMID:19245211]
ChEMBL	Agonist activity at adrenergic beta2 receptor in Hartley guinea pig tracheal ring assessed as bronchorelaxation activity against histamine-induced contraction	F	9.34	pEC50	0.45	nM	EC50	Eur J Med Chem (2009) 44: 2840-2846 [PMID:19168263]
GroEL/GroES in Escherichia coli (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4106139] [UniProtKB: Q548M1, Q7BGE6]
ChEMBL	Inhibition of Escherichia coli GroEL expressed in Escherichia coliDH5alpha/Escherichia coli GroES expressed in Escherichia coli BL21 (DE3) assessed as reduction in GroEL/GroES-mediated denatured soluble pig heart MDH refolding by measuring MDH enzyme activity using sodium mesoxalate as substrate after 20 to 40 mins by malachite green dye based spectrometric analysis	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem Lett (2019) 29: 1106-1112 [PMID:30852084]
HSP60/HSP10 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4106131] [UniProtKB: P10809, P61604]
ChEMBL	Inhibition of human N-terminal octa-His-tagged HSP60 expressed in Escherichia coli Rosetta(DE3) pLysS/human HSP10 expressed in Escherichia coli Rosetta(DE3) assessed as reduction in HSP60/HSP10-mediated denatured MDH refolding by measuring MDH enzyme activity using sodium mesoxalate as substrate after 40 to 60 mins by malachite green dye based spectrometric analysis	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem Lett (2019) 29: 1106-1112 [PMID:30852084]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]