ChEMBL ligand: CHEMBL565789
Leukotriene A4 hydrolase/Leukotriene A4 hydrolase in Human [ChEMBL: CHEMBL4618] [GtoPdb: 1395] [UniProtKB: P09960] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Leukotriene A4 hydrolase/Leukotriene A4 hydrolase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4618] [GtoPdb: 1395] [UniProtKB: P09960]
ChEMBL	Binding affinity to human recombinant LTA4H by isothermal calorimetry	B	7.6	pKd	25	nM	Kd	J Med Chem (2010) 53: 573-585 [PMID:19950900]
ChEMBL	Inhibition of LTA4H in human whole blood assessed as reduction of calcium ionophore A23187-stimulated LTB4 generation preincubated for 4 hrs followed by ionophore stimulation and measured after 15 hrs by EIA assay	B	6.29	pIC50	510	nM	IC50	J Med Chem (2021) 64: 1889-1903 [PMID:33592148]
ChEMBL	Inhibition of LTA4H in human PBMC assessed as reduction of calcium ionophore A23187-stimulated LTB4 generation preincubated for 4 hrs followed by ionophore stimulation and measured after 2 hrs by EIA assay	B	7.13	pIC50	74	nM	IC50	J Med Chem (2021) 64: 1889-1903 [PMID:33592148]
ChEMBL	Inhibition of recombinant full length N-terminal His6-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21 DE3 cells at enzyme concentration <1 nM using Arg-AMC as substrate preincubated for 15 mins followed by substrate addition and measured every 10 mins for 300 mins by fluorescence based assay	B	7.27	pIC50	54	nM	IC50	J Med Chem (2021) 64: 1889-1903 [PMID:33592148]
GtoPdb	-	-	7.3	pIC50	47	nM	IC50	J Med Chem (2010) 53: 573-85 [PMID:19950900]
ChEMBL	Inhibition of human recombinant LTA4H hydrolysis assessed as inhibition of LTB4 formation by LC-MS/MS	B	7.33	pIC50	47	nM	IC50	J Med Chem (2010) 53: 573-585 [PMID:19950900]
ChEMBL	Inhibition of human LTA4H hydrolysis assessed as inhibition of Ca2+ ionophore-stimulated LTB4 formation in human whole blood by ELISA	B	7.43	pIC50	37	nM	IC50	J Med Chem (2010) 53: 573-585 [PMID:19950900]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]