ChEMBL ligand: CHEMBL710 (Aindeem, Finasteride, MK-906, NSC-741485, NSC-759318, Propecia, Proscar, Prostide)
3-beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type I in Human [ChEMBL: CHEMBL1958] [UniProtKB: P14060] There should be some charts here, you may need to enable JavaScript!
Androgen receptor/Androgen Receptor in Rat [ChEMBL: CHEMBL3072] [GtoPdb: 628] [UniProtKB: P15207] There should be some charts here, you may need to enable JavaScript!
CYP2C19/Cytochrome P450 2C19 in Human [ChEMBL: CHEMBL3622] [GtoPdb: 1328] [UniProtKB: P33261] There should be some charts here, you may need to enable JavaScript!
CYP2C9/Cytochrome P450 2C9 in Human [ChEMBL: CHEMBL3397] [GtoPdb: 1326] [UniProtKB: P11712] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
Steroid 5-alpha-reductase 1 in Human [ChEMBL: CHEMBL1787] [UniProtKB: P18405] Steroid 5-alpha-reductase 1 in Rat [ChEMBL: CHEMBL3422] [UniProtKB: P24008] There should be some charts here, you may need to enable JavaScript!
steroid 5 alpha-reductase 2/Steroid 5-alpha-reductase 2 in Human [ChEMBL: CHEMBL1856] [GtoPdb: 2633] [UniProtKB: P31213] steroid 5 alpha-reductase 2/Steroid 5-alpha-reductase 2 in Rat [ChEMBL: CHEMBL5099] [GtoPdb: 2633] [UniProtKB: P31214] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
3-beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type I in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1958] [UniProtKB: P14060]
ChEMBL	Binding affinity for 3-beta-hydroxysteroid dehydrogenase	B	4.96	pKi	11000	nM	Ki	J Med Chem (1997) 40: 1293-1315 [PMID:9135028]
Androgen receptor/Androgen Receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3072] [GtoPdb: 628] [UniProtKB: P15207]
ChEMBL	In vitro antagonist activity against rat prostatic androgen receptor (AR)	F	4	pIC50	>100000	nM	IC50	J Med Chem (1986) 29: 2298-2315 [PMID:3783591]
CYP2C19/Cytochrome P450 2C19 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3622] [GtoPdb: 1328] [UniProtKB: P33261]
ChEMBL	DRUGMATRIX: CYP450, 2C19 enzyme inhibition (substrate: 3-Cyano-7-ethoxycoumarin)	B	6	pIC50	1000	nM	IC50	DrugMatrix in vitro pharmacology data
CYP2C9/Cytochrome P450 2C9 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3397] [GtoPdb: 1326] [UniProtKB: P11712]
ChEMBL	DRUGMATRIX: CYP450, 2C9 enzyme inhibition (substrate: 3-Cyano-7-ethoxycoumarin)	B	5.7	pIC50	2000	nM	IC50	DrugMatrix in vitro pharmacology data
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum 7G8 after 72 hrs by SYBR green assay	F	5	pIC50	10000	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum W2 after 72 hrs by SYBR green assay	F	5	pIC50	10000	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum GB4 after 72 hrs by SYBR green assay	F	5.1	pIC50	7943.28	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
Steroid 5-alpha-reductase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1787] [UniProtKB: P18405]
ChEMBL	Inhibitory activity against recombinant Steroid 5-alpha-reductase type I expressed in CHO cells	B	6.44	pKi	366	nM	Ki	J Med Chem (2000) 43: 3718-3735 [PMID:11020287]
ChEMBL	Binding affinity for human 5 alpha reductase 1 isozyme	B	6.82	pKi	150	nM	Ki	J Med Chem (1997) 40: 1293-1315 [PMID:9135028]
ChEMBL	Apparent inhibition constant towards human Steroid 5-alpha-reductase type I	B	7	pKi	100	nM	Ki	Bioorg Med Chem Lett (1994) 4: 2327-2330
ChEMBL	Inhibitory activity against 5-alpha-reductase type 1 in cell culture system using LNCaP cells (androgen-sensitive human prostatic cancer cell line)	B	4.28	pIC50	53000	nM	IC50	Bioorg Med Chem Lett (2001) 11: 2361-2364 [PMID:11527731]
ChEMBL	Inhibitory concentration against human recombinant steroid 5-alpha-reductase type I in stably transfected chinese hamster ovary (CHO) 1827 cells using [3H]testosterone.	B	6.04	pIC50	911	nM	IC50	J Med Chem (2004) 47: 3546-3560 [PMID:15214782]
ChEMBL	Compound was evaluated for the inhibition of human Steroid 5-alpha-reductase type 1 from recombinant CHO cells	B	6.04	pIC50	911	nM	IC50	Bioorg Med Chem Lett (1998) 8: 2871-2876 [PMID:9873639]
ChEMBL	Inhibition of human type 1 5alpha reductase expressed in HEK293 cells	B	6.19	pIC50	650	nM	IC50	J Med Chem (2018) 61: 5822-5880 [PMID:29400967]
ChEMBL	Inhibition of Human steroid 5-alpha-reductase type I expressed in HEK293 cells	B	6.27	pIC50	540	nM	IC50	J Med Chem (2000) 43: 4266-4277 [PMID:11063622]
ChEMBL	Inhibitory activity against type-1 human steroid 5-alpha-reductase.	B	6.3	pIC50	500	nM	IC50	Bioorg Med Chem Lett (1996) 6: 481-484
ChEMBL	Inhibition of human 5alpha-1 reductase expressed in HEK293 cells assessed as suppression of conversion of [3]androstenedione incubated for 30 mins by radioactivity-based HPLC analysis	B	6.34	pIC50	453	nM	IC50	Bioorg Med Chem (2016) 24: 779-788 [PMID:26780831]
ChEMBL	Inhibition of human type-1 5-alpha reductase expressed in HEK293 cells using [3H]-androstenedione as substrate after 30 mins by HPLC analysis	B	6.34	pIC50	453	nM	IC50	Eur J Med Chem (2012) 54: 728-739 [PMID:22776417]
ChEMBL	Inhibition of steroid 5-alpha reductase type 1 expressed in HEK 293 cells	B	6.34	pIC50	453	nM	IC50	Bioorg Med Chem Lett (2007) 17: 3603-3607 [PMID:17490876]
ChEMBL	In vitro inhibition of human Steroid 5-alpha-reductase type I in transfected 293 cells using [3H]- delta4-Androstenedione as substrate	B	6.66	pIC50	218	nM	IC50	J Med Chem (1995) 38: 1456-1461 [PMID:7739004]
ChEMBL	Inhibition of human type 1 5alpha reductase	B	6.82	pIC50	150	nM	IC50	J Med Chem (2018) 61: 5822-5880 [PMID:29400967]
ChEMBL	Binding affinity to recombinant human Steroid 5-alpha-reductase type I was evaluated	B	6.82	pIC50	150	nM	IC50	J Med Chem (1995) 38: 2621-2627 [PMID:7629802]
ChEMBL	In vitro inhibitory activity against human type 1 5-alpha reductase	B	6.82	pIC50	150	nM	IC50	J Med Chem (1994) 37: 2352-2360 [PMID:8057283]
ChEMBL	Inhibition of recombinant human 5-alpha reductase-1 at a concentration of 5 microL after pre-incubation for 10 minutes	B	6.82	pIC50	150	nM	IC50	J Med Chem (1993) 36: 4313-4315 [PMID:8277514]
ChEMBL	Compound was tested for inhibition of Type I 5-alpha-reductase in Human genital skin (Hs68) foreskin fibroblast cells.	B	7.21	pIC50	62	nM	IC50	J Med Chem (1993) 36: 421-423 [PMID:8381185]
ChEMBL	Inhibition of [1-beta-3H]-androstenedione binding to human steroid 5-alpha-reductase type I expressed in DU-145 cells at 10 uM	F	7.22	pIC50	60	nM	IC50	J Med Chem (2005) 48: 2972-2984 [PMID:15828836]
ChEMBL	Inhibition of recombinant human Steroid 5-alpha-reductase type I	B	7.28	pIC50	52	nM	IC50	J Med Chem (1995) 38: 3189-3192 [PMID:7650670]
ChEMBL	Inhibition of type 1 steroid-5-alpha-reductase	B	7.28	pIC50	52	nM	IC50	J Med Chem (1994) 37: 3871-3874 [PMID:7966146]
ChEMBL	Inhibition of type 1 5alpha reductase in human prostate	B	7.28	pIC50	52	nM	IC50	J Med Chem (2018) 61: 5822-5880 [PMID:29400967]
ChEMBL	In vitro inhibitory activity against Steroid 5-alpha-reductase type I of human DU 145 prostatic tumor cell line	B	7.39	pIC50	41	nM	IC50	J Med Chem (2002) 45: 3406-3417 [PMID:12139451]
ChEMBL	Evaluated for the inhibitory activity against human steroid 5-alpha-reductase type I in human DU-145 cell assay at 5 nM of androstenedione	B	7.4	pIC50	40	nM	IC50	J Med Chem (2000) 43: 4266-4277 [PMID:11063622]
ChEMBL	In vitro inhibition of human steroid 5-alpha-reductase type I in Du-145 cells	B	7.58	pIC50	26.3	nM	IC50	J Med Chem (1995) 38: 1158-1173 [PMID:7707319]
Steroid 5-alpha-reductase 1 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3422] [UniProtKB: P24008]
ChEMBL	Inhibition of recombinant Steroid 5-alpha-reductase type I was evaluated as binding affinity (in vitro)	B	8.17	pKi	6.8	nM	Ki	J Med Chem (1995) 38: 2621-2627 [PMID:7629802]
ChEMBL	Compound was tested for its inhibitory potency against rat Steroid 5-alpha-reductase type 1 expressed in transformed yeast Saccharomyces cerevisiae	B	4.4	pIC50	40000	nM	IC50	Bioorg Med Chem Lett (1996) 6: 1997-2002
ChEMBL	Inhibition of type 1 5alpha reductase in rat prostate	B	7.52	pIC50	30	nM	IC50	J Med Chem (2018) 61: 5822-5880 [PMID:29400967]
ChEMBL	Inhibition of Steroid 5-alpha-reductase type I from rat ventral prostate (RVP)	B	8	pIC50	10	nM	IC50	J Med Chem (2002) 45: 3406-3417 [PMID:12139451]
ChEMBL	Compound was tested for the inhibitory activity against Steroid 5-alpha-reductase type 1 in rat	B	8.38	pIC50	4.2	nM	IC50	Bioorg Med Chem Lett (1998) 8: 409-414 [PMID:9871588]
steroid 5 alpha-reductase 2/Steroid 5-alpha-reductase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1856] [GtoPdb: 2633] [UniProtKB: P31213]
ChEMBL	Apparent inhibition constant towards human Steroid 5-alpha-reductase type 2	B	8.7	pKi	2	nM	Ki	Bioorg Med Chem Lett (1994) 4: 2327-2330
ChEMBL	Inhibition of Human steroid 5-alpha-reductase type II expressed in HEK293 cells	B	7.22	pIC50	60	nM	IC50	J Med Chem (2000) 43: 4266-4277 [PMID:11063622]
ChEMBL	Inhibition of type 2 5alpha reductase in human prostate	B	7.24	pIC50	58	nM	IC50	J Med Chem (2018) 61: 5822-5880 [PMID:29400967]
ChEMBL	Inhibitory activity against human 5-alpha Reductase-1 expressed in DU-145 cells	B	7.38	pIC50	42	nM	IC50	J Med Chem (1997) 40: 1112-1129 [PMID:9089333]
ChEMBL	Inhibition of human 5alpha-2 reductase expressed in HEK293 cells assessed as suppression of conversion of [3]androstenedione incubated for 30 mins by radioactivity-based HPLC analysis	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem (2016) 24: 779-788 [PMID:26780831]
ChEMBL	Inhibition of human prostate 5alpha-reductase type 2 assessed as formation dihydrotestosterone from [4-14C] testosterone	B	7.46	pIC50	35	nM	IC50	Bioorg Med Chem Lett (2011) 21: 475-478 [PMID:21094046]
ChEMBL	Inhibition of human type-2 5-alpha reductase expressed in HEK293 cells using [3H]-androstenedione as substrate after 30 mins by HPLC analysis	B	7.52	pIC50	30.3	nM	IC50	Eur J Med Chem (2012) 54: 728-739 [PMID:22776417]
ChEMBL	In Vitro Inhibition Assay: This work was performed at the MDS Pharma Services, Pharmacology Laboratories, Taiwan. The assay was an in vitro evaluation of the ability of an extract or a pure compound to inhibit the steroid 5alpha -reductase enzyme from metabolizing testosterone into dihydrotestosterone. This is an enzyme-immunoassay (EIA) for quantitative determination of testosterone in human serum or plasma. The significance of this type of inhibition is that it can lead to eradication of benign prostatic hyperplasia (BPH). Two distinct isozymes are found in mice, rats, monkeys and humans: type 1 and II. Each of these isozymes is differentially expressed in tissues and developmental stages. In human, type 1 steroid 5alpha -reductase is predominant in the sebaceous glands of most regions of skin, including scalp and liver and is responsible for approximately one third of circulating DHT. Inhibitors of steroid 5alpha -reductase may be of benefit in the treatment of androgenetic alopecia.	B	7.6	pIC50	25	nM	IC50	US-9061023-B2. Management and treatment of benign prostatic hyperplasia (2015)
ChEMBL	Inhibition of steroid 5-alpha reductase type 2 expressed in HEK 293 cells	B	7.6	pIC50	25	nM	IC50	Bioorg Med Chem Lett (2007) 17: 3603-3607 [PMID:17490876]
ChEMBL	Inhibitory concentration against human recombinant steroid 5-alpha-reductase type 2 in stably transfected chinese hamster ovary 1827 cells using [3H]testosterone	B	7.68	pIC50	21	nM	IC50	J Med Chem (2004) 47: 3546-3560 [PMID:15214782]
GtoPdb	-	-	7.84	pIC50	14.6	nM	IC50	J Med Chem (1986) 29: 2298-315 [PMID:3783591]
ChEMBL	Compound was tested for inhibition of Type II 5-alpha-reductase in Human Prostate Homogenates (HPH).	B	8	pIC50	10	nM	IC50	J Med Chem (1993) 36: 421-423 [PMID:8381185]
ChEMBL	Inhibition of human prostate type 2 5alpha-reductase assessed as transformation of testosterone to dihydrotestosterone	B	8.07	pIC50	8.5	nM	IC50	Bioorg Med Chem (2015) 23: 7535-7542 [PMID:26631442]
ChEMBL	Inhibition of human prostate 5alpha-reductase type 2 assessed as conversion of [3H]testosterone to dihydrotestosterone	B	8.07	pIC50	8.5	nM	IC50	Bioorg Med Chem (2014) 22: 6233-6241 [PMID:25261928]
ChEMBL	In vitro inhibition of human Steroid 5-alpha-reductase type 2 in transfected SW-13 cells using [3H]- delta4-Androstenedione as substrate	B	8.07	pIC50	8.47	nM	IC50	J Med Chem (1995) 38: 1456-1461 [PMID:7739004]
ChEMBL	Inhibition of Steroid 5-alpha-reductase type 2 from human benign prostatic hyperplasia (BPH) tissue	B	8.3	pIC50	5	nM	IC50	J Med Chem (2002) 45: 3406-3417 [PMID:12139451]
ChEMBL	In vitro inhibition of human steroid 5-alpha-reductase type 2 in SW-13-transfected cells	B	8.34	pIC50	4.53	nM	IC50	J Med Chem (1995) 38: 1158-1173 [PMID:7707319]
ChEMBL	Inhibitory activity against type-2 human steroid 5-alpha-reductase.	B	8.38	pIC50	4.2	nM	IC50	Bioorg Med Chem Lett (1996) 6: 481-484
ChEMBL	Evaluated for the inhibitory activity against human steroid 5-alpha-reductase type 2 from human BPH tissue at 210 nM of testosterone	B	8.52	pIC50	3	nM	IC50	J Med Chem (2000) 43: 4266-4277 [PMID:11063622]
ChEMBL	Inhibitory activity against 5-alpha Reductase-2 on human prostate homogenates from surgically derived benign hyperplastic tissue	B	8.52	pIC50	3	nM	IC50	J Med Chem (1997) 40: 1112-1129 [PMID:9089333]
ChEMBL	Inhibitory activity against steroid 5-alpha-reductase type 2 in human prostate homogenates	B	8.66	pIC50	2.2	nM	IC50	J Med Chem (2000) 43: 3718-3735 [PMID:11020287]
ChEMBL	Inhibition of [1-beta-2beta-3H]- -testosterone binding to human steroid 5-alpha-reductase type 2 of BPH tissue at 10 uM	F	8.7	pIC50	2	nM	IC50	J Med Chem (2005) 48: 2972-2984 [PMID:15828836]
ChEMBL	Compound was evaluated for the inhibition of human Steroid 5-alpha-reductase type 2 receptor from human prostate homogenates	B	8.92	pIC50	1.2	nM	IC50	Bioorg Med Chem Lett (1998) 8: 2871-2876 [PMID:9873639]
ChEMBL	Inhibition of human type 2 5alpha reductase expressed in HEK293 cells	B	8.92	pIC50	1.2	nM	IC50	J Med Chem (2018) 61: 5822-5880 [PMID:29400967]
ChEMBL	Inhibition of human type 2 5alpha reductase	B	9.74	pIC50	0.18	nM	IC50	J Med Chem (2018) 61: 5822-5880 [PMID:29400967]
ChEMBL	Inhibition of recombinant human 5-alpha reductase-2 at a concentration of 5 microL after pre-incubation for 10 minutes	B	9.74	pIC50	0.18	nM	IC50	J Med Chem (1993) 36: 4313-4315 [PMID:8277514]
ChEMBL	Inhibitory activity measured on human steroid 5-alpha-reductase type 2	B	9.74	pIC50	0.18	nM	IC50	J Med Chem (1995) 38: 2621-2627 [PMID:7629802]
ChEMBL	In vitro inhibitory activity against human type 2 5-alpha reductase	B	9.74	pIC50	0.18	nM	IC50	J Med Chem (1994) 37: 2352-2360 [PMID:8057283]
ChEMBL	Binding affinity for human 5-alpha reductase 2 isozyme	B	9.74	pIC50	0.18	nM	IC50	J Med Chem (1997) 40: 1293-1315 [PMID:9135028]
ChEMBL	Inhibition of recombinant human Steroid 5-alpha-reductase type 2	B	10	pIC50	<0.1	nM	IC50	J Med Chem (1995) 38: 3189-3192 [PMID:7650670]
ChEMBL	Inhibition of type 2 steroid-5-alpha-reductase	B	10	pIC50	<0.1	nM	IC50	J Med Chem (1994) 37: 3871-3874 [PMID:7966146]
steroid 5 alpha-reductase 2/Steroid 5-alpha-reductase 2 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5099] [GtoPdb: 2633] [UniProtKB: P31214]
ChEMBL	Compound was tested in vitro for inhibitory activity against type 2 5-alpha reductase Isozyme homogenated from rat epididymis	F	5.68	pIC50	2100	nM	IC50	J Med Chem (1996) 39: 5047-5052 [PMID:8978835]
ChEMBL	Inhibition of rat 5-alpha-reductase type 2 by Lineweaver-Burk plot	B	6.83	pIC50	147.49	nM	IC50	Bioorg Med Chem Lett (2011) 21: 3439-3442 [PMID:21515045]
ChEMBL	Inhibition of type 2 5alpha reductase in rat prostate	B	7.49	pIC50	32	nM	IC50	J Med Chem (2018) 61: 5822-5880 [PMID:29400967]
ChEMBL	Inhibition of Steroid 5-alpha-reductase type 2 of rat ventral prostate (RVP)	B	7.96	pIC50	11	nM	IC50	J Med Chem (2002) 45: 3406-3417 [PMID:12139451]
ChEMBL	Inhibitory activity was measured on rat Steroid 5-alpha-reductase type 2	B	9.55	pIC50	0.28	nM	IC50	J Med Chem (1995) 38: 2621-2627 [PMID:7629802]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]