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ChEMBL ligand: CHEMBL473417 (Erivedge, GDC-0449, NSC-747691, NSC-755986, Vismodegib) |
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DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
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Kv11.1/HERG in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809] | ||||||||
ChEMBL | Inhibition of human ERG | B | 4.48 | pIC50 | >33000 | nM | IC50 | Bioorg Med Chem (2020) 28: 115227-115227 [PMID:31862310] |
SMO/Smoothened homolog in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5971] [GtoPdb: 239] [UniProtKB: Q99835] | ||||||||
ChEMBL | Binding affinity to recombinant human Smo receptor assessed as dissociation constant at equilibrium by SPR analysis | B | 7.01 | pKd | 97.5 | nM | Kd | Bioorg Med Chem (2020) 28: 115584-115584 [PMID:32690258] |
GtoPdb | - | - | 7.79 | pKi | 16.2 | nM | Ki | Bioorg Med Chem (2012) 20: 6751-7 [PMID:23063522] |
ChEMBL | Displacement of [3H]cyclopamine from wild type Smo expressed in U2OS cells after 2 hrs by scintillation counting | B | 7.79 | pKi | 16.2 | nM | Ki | Bioorg Med Chem (2012) 20: 6751-6757 [PMID:23063522] |
ChEMBL | Displacement of [3H]-cyclopamine from SMO V404M mutant in gefitinib resistant human HCC827 cells by scintillation counting | B | 7.91 | pKi | 12.2 | nM | Ki | J Med Chem (2017) 60: 7447-7458 [PMID:28787156] |
ChEMBL | Displacement of BODIPY-Cyclopamine from human HA-tagged Smo receptor expressed in human U2OS cells at 1 to 10000 nM incubated for 2 hrs by DAPI staining based fluorescence microscopic method | B | 7.13 | pIC50 | 74 | nM | IC50 | Eur J Med Chem (2019) 172: 1-15 [PMID:30939349] |
ChEMBL | Antagonist activity at smoothened (unknown origin) expressed in mouse Shh Light2 cells co-expressing Gli-dependent reporter gene assessed as inhibition of Hh signaling by dual luciferase reporter gene assay | F | 7.48 | pIC50 | 33 | nM | IC50 | Bioorg Med Chem Lett (2014) 24: 1426-1431 [PMID:24491459] |
ChEMBL | Inhibition of Smo receptor (unknown origin) expressed in NIH3T3 cells assessed as inhibition of Smo agonist SAG-induced GRE activation after 30 hrs by luciferase reporter gene assay | B | 7.64 | pIC50 | 23 | nM | IC50 | Bioorg Med Chem Lett (2014) 24: 2300-2304 [PMID:24726807] |
ChEMBL | Inhibition of smoothened (unknown origin)-mediated Shh signaling | B | 7.7 | pIC50 | 20 | nM | IC50 | Bioorg Med Chem Lett (2014) 24: 1426-1431 [PMID:24491459] |
ChEMBL | Inhibition of SMO D473H mutant receptor (unknown origin) assessed as inhibition of SAG-induced Hh signaling pathway by Gli luciferase reporter cell-based assay | B | 7.88 | pIC50 | 13.06 | nM | IC50 | Bioorg Med Chem (2020) 28: 115354-115354 [PMID:32063403] |
ChEMBL | Inhibition of Smo-mediated Hh signaling in human Shh-light2 cells by luciferase reporter gene assay | F | 8.15 | pIC50 | 7 | nM | IC50 | J Med Chem (2012) 55: 1559-1571 [PMID:22268551] |
ChEMBL | Displacement of BODIPY-labelled cyclopamine from human Smo receptor expressed in HEK293 cells after 2 hrs by fluorescence microscopy | B | 8.15 | pIC50 | 7 | nM | IC50 | J Med Chem (2012) 55: 1559-1571 [PMID:22268551] |
ChEMBL | Displacement of boron-dipyrromethene-cyclopamine from human smoothened receptor expressed in HEK293 cells incubated for 4 hrs by hSMO-BC binding assay | B | 8.22 | pIC50 | 6 | nM | IC50 | Medchemcomm (2015) 6: 1137-1142 |
ChEMBL | Displacement of BODIPY-cyclopamine from human Smo expressed in HEK293 cells incubated for 3 hrs by fluorescence competitive displacement assay | B | 8.29 | pIC50 | 5.1 | nM | IC50 | Medchemcomm (2016) 7: 960-965 |
ChEMBL | Inhibition of SMO-mediated hedgehog signalling pathway in human HPEM cells by luciferase reporter gene assay | B | 9.4 | pIC50 | 0.4 | nM | IC50 | Bioorg Med Chem (2020) 28: 115227-115227 [PMID:31862310] |
ChEMBL | Displacement of BODIPY-labeled cyclopamine from human SMO transfected in human HeLa cells by competition binding assay | B | 8.52 | pEC50 | 3 | nM | EC50 | Bioorg Med Chem (2020) 28: 115227-115227 [PMID:31862310] |
ChEMBL | Binding affinity to human SMO | B | 8.7 | pEC50 | 2 | nM | EC50 | Bioorg Med Chem (2020) 28: 115227-115227 [PMID:31862310] |
SMO/Smoothened homolog in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6080] [GtoPdb: 239] [UniProtKB: P56726] | ||||||||
ChEMBL | Antagonist activity at SMO in mouse NIH3T3 cells preincubated for 2 hrs followed by SAG stimulation and measured after 24 hrs by Bright-Glo luciferase assay | F | 6.4 | pIC50 | 400 | nM | IC50 | J Med Chem (2021) 64: 13830-13840 [PMID:34492176] |
ChEMBL | Antagonist activity at Smo receptor in mouse NIH/3T3 cells harboring GRE-Luc assessed as inhibition of SAG-induced Hh signaling pathway preincubated with cells followed by SAG addition measured after 48 hrs by luciferase reporter gene assay | F | 7.34 | pIC50 | 46 | nM | IC50 | Eur J Med Chem (2018) 155: 34-48 [PMID:29857275] |
ChEMBL | Antagonist activity at Smo receptor in mouse NIH/3T3 cells harboring GRE-Luc assessed as inhibition of SAG-induced Hh signaling pathway preincubated with cells followed by SAG addition measured after 48 hrs by luciferase reporter gene assay | B | 7.34 | pIC50 | 46 | nM | IC50 | Eur J Med Chem (2019) 172: 1-15 [PMID:30939349] |
ChEMBL | Inhibition of Smo-mediated Hh signalling pathway in mouse Shh Light2 cells by Gli-luciferase reporter gene assay | B | 7.41 | pIC50 | 39.2 | nM | IC50 | J Med Chem (2016) 59: 11050-11068 [PMID:27736063] |
ChEMBL | Inhibition of SMO in mouse NIH/3T3 cells assessed as inhibition of SAG-induced hedgehog-mediated luminescence signaling by GRE-luciferase reporter gene assay | B | 7.8 | pIC50 | 16 | nM | IC50 | Bioorg Med Chem Lett (2015) 25: 3665-3670 [PMID:26119500] |
ChEMBL | Inhibition of smo-mediated hedgehog signaling pathway in mouse NIH3T3 cells expressing GRE-Luc reporter gene assessed as inhibition of SAG-induced GRE-Luc reporter activity after 48 hrs by luminescence assay | F | 8.22 | pIC50 | 6 | nM | IC50 | Eur J Med Chem (2018) 149: 110-121 [PMID:29499483] |
ChEMBL | Inhibition of Smo in mouse C3H10T1/2 cells using human recombinant SHH assessed as effect on SMO/SHH transient transcriptional activation after 20 hrs by Gli-luciferase reporter assay | B | 8.3 | pIC50 | 5 | nM | IC50 | ACS Med Chem Lett (2012) 3: 106-111 [PMID:24900436] |
ChEMBL | Antagonist activity at Smo in mouse Shh-Light 2 cells assessed as inhibition of Shh-induced Gli1-reporter activity after 2 days by dual-luciferase reporter gene method | F | 8.82 | pIC50 | 1.5 | nM | IC50 | Bioorg Med Chem (2012) 20: 6751-6757 [PMID:23063522] |
ChEMBL | Inhibition of SMO-mediated Hedgehog signaling pathway in mouse C3H10T1/2 cells assessed as reduction in sonic hedgehog-induced osteoblast differentiation by measuring alkaline phosphatase activity incubated for 72 hrs | B | 8.33 | pEC50 | 4.7 | nM | EC50 | Bioorg Med Chem (2020) 28: 115227-115227 [PMID:31862310] |
ChEMBL | Displacement of BODIPY-labeled cyclopamine from mouse SMO transfected in human HeLa cells by competition binding assay | B | 8.7 | pEC50 | 2 | nM | EC50 | Bioorg Med Chem (2020) 28: 115227-115227 [PMID:31862310] |
Sonic hedgehog protein in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5602] [UniProtKB: Q15465] | ||||||||
ChEMBL | Inhibition of human SHH pathway in mouse S12 cells assessed as GLI-mediated transcriptional activity after 48 hrs by luciferase reporter gene assay in presence of 0.5 mg/mL human alpha-1-acid glycoprotein | B | 5.76 | pIC50 | 1750 | nM | IC50 | J Med Chem (2011) 54: 2592-2601 [PMID:21438527] |
ChEMBL | Inhibition of human SHH pathway in mouse S12 cells assessed as GLI-mediated transcriptional activity after 48 hrs by luciferase reporter gene assay | B | 7.82 | pIC50 | 15 | nM | IC50 | J Med Chem (2011) 54: 2592-2601 [PMID:21438527] |
Sonic hedgehog protein in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5387] [UniProtKB: Q62226] | ||||||||
ChEMBL | Inhibition of hedgehog signalling in mouse C3H10T1/2 cells assessed as reduction in Smo agonist SAG induced cell differentiation into alkaline phosphatase-positive osteoblasts using pNp substrate incubated for 72 hrs | B | 7.77 | pIC50 | 17 | nM | IC50 | Medchemcomm (2015) 6: 1137-1142 |
ChEMBL | Inhibition of SHH in mouse C3H10T1/2 cells by Gli-luciferase reporter gene assay | B | 7.89 | pIC50 | 13 | nM | IC50 | Bioorg Med Chem Lett (2009) 19: 5576-5581 [PMID:19716296] |
ChEMBL | Inhibition of hedgehog signalling in mouse NIH3T3-Gli-luc cells after 48 hrs by dual luciferase reporter assay | B | 7.89 | pIC50 | 13 | nM | IC50 | Medchemcomm (2015) 6: 1137-1142 |
ChEMBL | Inhibition of SHH signaling pathway in mouse NIH3T3 cells measured after 48 hrs by Gli-luciferase reporter assay | B | 8.14 | pIC50 | 7.2 | nM | IC50 | Bioorg Med Chem Lett (2013) 23: 6777-6783 [PMID:24176396] |
ChEMBL | Inhibition of SHH in mouse Shh Light2 cells by GLI-responsive firefly luciferase reporter gene assay | F | 8.52 | pIC50 | 3 | nM | IC50 | J Med Chem (2009) 52: 3829-3845 [PMID:19309080] |
ChEMBL data shown on this page come from version 33:
Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]