sofosbuvir [Ligand Id: 7368] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL1259059 (GS-7977, GS7977, PSI-7977, Psi-7977, gs-7977, Sofosbuvir, Sovaldi)
  • Cereblon isoform 4 in Magnetospirillum gryphiswaldense [ChEMBL: CHEMBL3763007] [UniProtKB: A4TVL0]
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  • Hepatitis C virus NS5B RNA-dependent RNA polymerase in Hepatitis C virus [ChEMBL: CHEMBL5375] [UniProtKB: Q8JXU8]
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DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
Cereblon isoform 4 in Magnetospirillum gryphiswaldense (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3763007] [UniProtKB: A4TVL0]
ChEMBL Binding affinity to Magnetospirillum gryphiswaldense cereblon isoform 4 by measuring baseline corrected normalized fluorescence by MST based assay B 4.57 pKi 27000 nM Ki ACS Med Chem Lett (2021) 12: 74-81 [PMID:33488967]
ChEMBL Binding affinity to Magnetospirillum gryphiswaldense cereblon isoform by FRET assay B 4.66 pKi 22000 nM Ki ACS Med Chem Lett (2021) 12: 74-81 [PMID:33488967]
Hepatitis C virus NS5B RNA-dependent RNA polymerase in Hepatitis C virus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5375] [UniProtKB: Q8JXU8]
ChEMBL Inhibition of NS5B S282T mutant in HCV genotype 1b replicon expressed in human HuH7 cells after 72 hrs by bright Glo-luciferase reporter gene assay B 6.23 pEC50 589 nM EC50 J Med Chem (2017) 60: 6077-6088 [PMID:28650160]
ChEMBL Inhibition of NS5B polymerase S282T mutant in HCV genotype 1b infected in human Huh7 replicon cells after 5 days by RT-PCR assay B 6.52 pEC50 300 nM EC50 J Med Chem (2019) 62: 1859-1874 [PMID:30653317]
ChEMBL Inhibition of NS5B polymerase in HCV genotype 1b Con1 infected in human HuH7 replicon cells assessed as inhibition of viral replication after 3 days by luciferase reporter gene assay B 6.64 pEC50 230 nM EC50 Bioorg Med Chem Lett (2020) 30: 126986-126986 [PMID:32046903]
ChEMBL Inhibition of NS5B polymerase in HCV genotype 1a H77 infected in human HuH7 replicon cells assessed as inhibition of viral replication after 3 days by luciferase reporter gene assay B 6.81 pEC50 155 nM EC50 Bioorg Med Chem Lett (2020) 30: 126986-126986 [PMID:32046903]
ChEMBL Inhibition of HCV genotype 1b NS5B RNA dependent RNA polymerase B 6.99 pEC50 102 nM EC50 Medchemcomm (2019) 10: 991-1006 [PMID:31303998]
ChEMBL Inhibition of Hepatitis C virus genotype 1b NS5B RNA-dependent-RNA polymerase assessed as reduction in viral replication after 72 hrs by indirect immunofluorescence assay B 7.19 pEC50 64.26 nM EC50 Bioorg Med Chem (2018) 26: 2621-2631 [PMID:29681484]
ChEMBL Inhibition of NS5B polymerase in HCV genotype 1b/3a chimeric replicon infected in human HuH7 replicon cells after 5 days by RT-PCR analysis B 7.19 pEC50 64 nM EC50 J Med Chem (2019) 62: 1859-1874 [PMID:30653317]
ChEMBL Inhibition of NS5B polymerase in HCV genotype 1a infected in human HuH7 replicon cells after 5 days by RT-PCR analysis B 7.21 pEC50 61 nM EC50 J Med Chem (2019) 62: 1859-1874 [PMID:30653317]
ChEMBL Inhibition of NS5B polymerase in HCV genotype 1b/4a chimeric replicon infected in human HuH7 replicon cells after 5 days by RT-PCR analysis B 7.24 pEC50 58 nM EC50 J Med Chem (2019) 62: 1859-1874 [PMID:30653317]
ChEMBL Inhibition of NS5B polymerase in HCV genotype 1b/5a chimeric replicon infected in human HuH7 replicon cells after 5 days by RT-PCR analysis B 7.36 pEC50 44 nM EC50 J Med Chem (2019) 62: 1859-1874 [PMID:30653317]
ChEMBL Inhibition of NS5B polymerase in HCV genotype 2a infected in human HuH7 replicon cells after 5 days by RT-PCR analysis B 7.37 pEC50 43 nM EC50 J Med Chem (2019) 62: 1859-1874 [PMID:30653317]
ChEMBL Inhibition of NS5B polymerase in wild type HCV genotype 1b infected in human Huh7 replicon cells after 5 days by RT-PCR assay B 7.47 pEC50 34 nM EC50 J Med Chem (2019) 62: 1859-1874 [PMID:30653317]
NS5 in Zika virus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523952] [UniProtKB: A0A143MHK7]
ChEMBL Inhibition of RNA-dependent RNA polymerase in Zika virus infected in African green monkey Vero cells assessed as antiviral activity by DAPI-staining based assay B 4.51 pEC50 30900 nM EC50 J Med Chem (2020) 63: 470-489 [PMID:31549836]
ChEMBL Inhibition of RNA-dependent RNA polymerase in Zika virus infected in human NPC assessed as antiviral activity by DAPI-staining based assay B 6.92 pEC50 120 nM EC50 J Med Chem (2020) 63: 470-489 [PMID:31549836]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]