ChEMBL ligand: CHEMBL224864
sirtuin 1/NAD-dependent deacetylase sirtuin 1 in Human [ChEMBL: CHEMBL4506] [GtoPdb: 2707] [UniProtKB: Q96EB6] There should be some charts here, you may need to enable JavaScript!
sirtuin 2/NAD-dependent deacetylase sirtuin 2 in Human [ChEMBL: CHEMBL4462] [GtoPdb: 2708] [UniProtKB: Q8IXJ6] There should be some charts here, you may need to enable JavaScript!
sirtuin 3/NAD-dependent deacetylase sirtuin 3 in Human [ChEMBL: CHEMBL4461] [GtoPdb: 2709] [UniProtKB: Q9NTG7] There should be some charts here, you may need to enable JavaScript!
sirtuin 5/NAD-dependent protein deacylase sirtuin-5, mitochondrial in Human [ChEMBL: CHEMBL2163183] [GtoPdb: 2711] [UniProtKB: Q9NXA8] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
sirtuin 1/NAD-dependent deacetylase sirtuin 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4506] [GtoPdb: 2707] [UniProtKB: Q96EB6]
ChEMBL	Inhibition of N-terminal His-tagged recombinant human SIRT-1 expressed in Escherichia coli using Fluor-de-Lys-SIRT1 as substrate after 15 mins by fluorescence assay	B	4.01	pIC50	98100	nM	IC50	J Med Chem (2017) 60: 4714-4733 [PMID:28475330]
ChEMBL	Inhibition of recombinant human N-terminal GST-tagged SIRT1 (193 to 741 residues) using human p53 derived peptides as substrate incubated for 45 mins by fluorescence assay	B	4.17	pIC50	68000	nM	IC50	Medchemcomm (2015) 6: 1857-1863
ChEMBL	Inhibition of human recombinant SIRT1 using fluorescent peptide substrate by fluorescent assay	B	4.3	pIC50	>50000	nM	IC50	Science (2007) 317: 516-519 [PMID:17588900]
ChEMBL	Inhibition of human recombinant SIRT1 by Flour de Lys assay	B	4.3	pIC50	>50000	nM	IC50	J Med Chem (2010) 53: 1407-1411 [PMID:20041717]
ChEMBL	Inhibition of human SIRT1	B	4.3	pIC50	>50000	nM	IC50	Bioorg Med Chem (2015) 23: 328-339 [PMID:25515955]
ChEMBL	Inhibition of human SIRT1	B	4.3	pIC50	>50000	nM	IC50	J Med Chem (2017) 60: 1928-1945 [PMID:28135086]
ChEMBL	Inhibition of human recombinant SIRT1 expressed in Escherichia coli cells using acetylated Lys side chain amino acids 379-382 (Arg-His-Lys-Lys(Ac)) p53 conjugated with aminomethylcoumarin as substrate by fluorescence assay	B	4.42	pIC50	38000	nM	IC50	J Med Chem (2012) 55: 8193-8197 [PMID:22931526]
ChEMBL	Inhibition of human recombinant SIRT1 using Fluor-de-Lys as substrate incubated for 60 mins prior to substrate addition measured after 60 mins by fluorometric analysis	B	4.52	pIC50	30000	nM	IC50	Bioorg Med Chem Lett (2014) 24: 1871-1874 [PMID:24675380]
ChEMBL	Inhibition of human recombinant SIRT1 using Fluor de Lys-SIRT as substrate incubated for 60 mins prior to substrate addition measured after 60 mins by fluorimetric analysis	B	4.52	pIC50	30000	nM	IC50	J Med Chem (2012) 55: 5760-5773 [PMID:22642300]
sirtuin 2/NAD-dependent deacetylase sirtuin 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4462] [GtoPdb: 2708] [UniProtKB: Q8IXJ6]
ChEMBL	Inhibition of GST-tagged SIRT2 (unknown origin) by HPLC assay	B	4.26	pIC50	55000	nM	IC50	Medchemcomm (2010) 1: 233-238
ChEMBL	Inhibition of human recombinant SIRT2 assessed as deacetylation of N-acetyl lysine residue in p53 (317 to 320) after 30 mins by luciferase-mediated luminescence assay	B	4.86	pIC50	13900	nM	IC50	Eur J Med Chem (2015) 92: 145-155 [PMID:25549554]
ChEMBL	Inhibition of human recombinant SIRT2 using AMC-p53 (317 to 320 residues) as substrate incubated for 45 mins followed by NAD+ addition measured after 30 mins by fluorescence assay	B	5.08	pIC50	8340	nM	IC50	Medchemcomm (2016) 7: 2094-2099
ChEMBL	Inhibition of human recombinant SIRT2 using p53 (317 to 320 residues) conjugated to AMC as substrate incubated for 45 mins in presence of NAD+ by fluorescence assay	B	5.08	pIC50	8340	nM	IC50	Medchemcomm (2019) 10: 2140-2145 [PMID:32904142]
ChEMBL	Inhibition of recombinant human C-terminal His-tagged SIRT2 (13 to 319 residues) using human p53 derived peptides as substrate incubated for 45 mins by fluorescence assay	B	5.1	pIC50	7900	nM	IC50	Medchemcomm (2015) 6: 1857-1863
ChEMBL	Inhibition of human recombinant SIRT2 expressed in Escherichia coli cells using acetylated Lys side chain amino acids 379-382 (Arg-His-Lys-Lys(Ac)) p53 conjugated with aminomethylcoumarin as substrate by fluorescence assay	B	5.26	pIC50	5500	nM	IC50	J Med Chem (2012) 55: 8193-8197 [PMID:22931526]
ChEMBL	Inhibition of human recombinant SIRT2 by Flour de Lys assay	B	5.45	pIC50	3540	nM	IC50	J Med Chem (2010) 53: 1407-1411 [PMID:20041717]
ChEMBL	Inhibition of human recombinant SIRT2	B	5.46	pIC50	3500	nM	IC50	Bioorg Med Chem (2015) 23: 328-339 [PMID:25515955]
ChEMBL	Inhibition of human SIRT2	B	5.46	pIC50	3500	nM	IC50	J Med Chem (2017) 60: 1928-1945 [PMID:28135086]
ChEMBL	Inhibition of SIRT-2 (unknown origin)	B	5.46	pIC50	3500	nM	IC50	Medchemcomm (2012) 3: 135-161
ChEMBL	Inhibition of SirT2	B	5.46	pIC50	3500	nM	IC50	Nat Chem Biol (2008) 4: 590-597 [PMID:18800048]
ChEMBL	Inhibition of SIRT2	B	5.46	pIC50	3500	nM	IC50	Bioorg Med Chem (2011) 19: 3616-3624 [PMID:21306906]
ChEMBL	Inhibition of Sirt2	B	5.46	pIC50	3500	nM	IC50	Bioorg Med Chem (2011) 19: 3605-3615 [PMID:21316248]
ChEMBL	Inhibition of human recombinant SIRT2 using Fluor de Lys-SIRT as substrate incubated for 60 mins prior to substrate addition measured after 60 mins by fluorimetric analysis	B	5.46	pIC50	3500	nM	IC50	J Med Chem (2012) 55: 5760-5773 [PMID:22642300]
ChEMBL	Inhibition of human recombinant SIRT2 using Fluor-de-Lys as substrate incubated for 60 mins prior to substrate addition measured after 60 mins by fluorometric analysis	B	5.46	pIC50	3500	nM	IC50	Bioorg Med Chem Lett (2014) 24: 1871-1874 [PMID:24675380]
ChEMBL	Inhibition of human recombinant SIRT2 using fluorescent peptide substrate by fluorescent assay	B	5.46	pIC50	3500	nM	IC50	Science (2007) 317: 516-519 [PMID:17588900]
ChEMBL	Inhibition of GST-tagged human recombinant SIRT2 (34 to 356 residues) expressed in Escherichia coli BL21 using Fluor de Lys-SIRT as substrate incubated for 60 mins prior to substrate addition measured after 45 mins by fluorimetric analysis	B	5.52	pIC50	3000	nM	IC50	Bioorg Med Chem (2015) 23: 328-339 [PMID:25515955]
ChEMBL	Inhibition of N-terminal His-tagged recombinant human SIRT-2 expressed in Escherichia coli using Fluor-de-Lys-SIRT1 as substrate after 15 mins by fluorescence assay	B	5.55	pIC50	2800	nM	IC50	J Med Chem (2017) 60: 4714-4733 [PMID:28475330]
ChEMBL	Inhibition of full length human SIRT2 expressed in Escherichia coli BL21 (DE3) cells using fluorogenic 7-amino-4-methylcoumarin (AMC)-labeled peptide by fluorescence assay	B	5.81	pIC50	1560	nM	IC50	J Med Chem (2014) 57: 8340-8357 [PMID:25275824]
GtoPdb	-	-	5.81	pIC50	1560	nM	IC50	Science (2007) 317: 516-9 [PMID:17588900]; J Med Chem (2014) 57: 8340-57 [PMID:25275824]
sirtuin 3/NAD-dependent deacetylase sirtuin 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4461] [GtoPdb: 2709] [UniProtKB: Q9NTG7]
ChEMBL	Inhibition of human recombinant SIRT3 using Fluor de Lys-SIRT as substrate incubated for 60 mins prior to substrate addition measured after 60 mins by fluorimetric analysis	B	4.04	pIC50	91000	nM	IC50	J Med Chem (2012) 55: 5760-5773 [PMID:22642300]
ChEMBL	Inhibition of human recombinant SIRT3 using Fluor-de-Lys as substrate incubated for 60 mins prior to substrate addition measured after 60 mins by fluorometric analysis	B	4.04	pIC50	91000	nM	IC50	Bioorg Med Chem Lett (2014) 24: 1871-1874 [PMID:24675380]
ChEMBL	Inhibition of catalytically active human SIRT3 (102 to 399 amino acids) expressed in Escherichia coli BL21 (DE3) cells using fluorogenic 7-amino-4-methylcoumarin (AMC)-labeled peptide by fluorescence assay	B	4.28	pIC50	52800	nM	IC50	J Med Chem (2014) 57: 8340-8357 [PMID:25275824]
ChEMBL	Inhibition of human recombinant SIRT3 using fluorescent peptide substrate by fluorescent assay	B	4.3	pIC50	>50000	nM	IC50	Science (2007) 317: 516-519 [PMID:17588900]
ChEMBL	Inhibition of human SIRT3	B	4.3	pIC50	>50000	nM	IC50	Bioorg Med Chem (2015) 23: 328-339 [PMID:25515955]
sirtuin 5/NAD-dependent protein deacylase sirtuin-5, mitochondrial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2163183] [GtoPdb: 2711] [UniProtKB: Q9NXA8]
ChEMBL	Inhibition of SIRT5	B	4	pIC50	>100000	nM	IC50	J Med Chem (2012) 55: 5582-5590 [PMID:22583019]
ChEMBL	Inhibition of human SIRT5 assessed as reduction in desuccinylase activity using KQTAR(SuK)STGGKA substrate	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem Lett (2015) 25: 1671-1674 [PMID:25818461]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]