ADGRE3 | Adhesion Class GPCRs | IUPHAR/BPS Guide to PHARMACOLOGY

ADGRE3

Target id: 184

Nomenclature: ADGRE3

Family: Adhesion Class GPCRs

Annotation status:  image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates.  » Email us

   GtoImmuPdb view: OFF :     Currently no data for ADGRE3 in GtoImmuPdb

Gene and Protein Information
Adhesion G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 652 19p13.12 ADGRE3 adhesion G protein-coupled receptor E3 8
Previous and Unofficial Names
EMR3 (EGF-like module-containing, mucin-like, hormone receptor-like 3)
Database Links
Specialist databases
GPCRDB agre3_human (Hs)
Other databases
Ensembl Gene
Entrez Gene
Human Protein Atlas
KEGG Gene
OMIM
RefSeq Nucleotide
RefSeq Protein
UniProtKB
Wikipedia
Agonist Comments
No ligands identified: orphan receptor.
Tissue Distribution
Highest levels in the spleen, peripheral blood leukocytes and lung, followed by intermediate levels in the placenta and bone marrow, with low detected levels in the heart, kidney, liver, pancreas, skeletal muscle, lymph node, thymus, tonsil and fetal liver.
With 40 cycles of amplification two monocyte/macrophage cell lines, 2 B cell lines and one T cell line were positive while 2 firbroblast lines were negative.
Species:  Human
Technique:  RT-PCR.
References:  8
Bone marrow, spleen, lung, placenta, peripheral blood (granulocyte fraction)
Species:  Human
Technique:  Northern blot and PCR
References:  8
Primary glioblastoma tissue
Species:  Human
Technique:  Immunohistochemistry
References:  2
Upregulated in mucosa asthmatic chronic rhinosinusitis with nasal polyps
Species:  Human
Technique:  Microarray analysis
References:  7
All types of myeloid cells including neutrophilic, basophilic and eosinophilic granulocytes (high expression), monocytes and myeloid dendritic cells (weak to intermediate expression), no expression in hematopoietic stem and progenitor cells and in immature granulocytes
Species:  Human
Technique:  Flow cytometry
References:  6
Functional Assays
Expression increases in vitro invasion of glioblastoma cell line
Species:  Human
Tissue:  SF-767 cell line
Response measured:  Elevated in vitro invasion
References:  2
Physiological Functions
Potential mediator of invasive phenotypic variation in glioblastoma
Species:  Human
Tissue:  Primary glioblastoma, SF-767 cell line
References:  2
General Comments
ADGRE3 (adhesion G protein-coupled receptor E3, formerly known as EMR3: EGF-like module containing, mucin-like, hormone receptor-like 3) is an orphan receptor that belongs to Family II Adhesion-GPCRs together with ADGRE5 and ADGRE1, ADGRE2 and ADGRE4 [1,3]. The genes of Family II Adhesion-GPCRs are syntenically clustered on human chromosome 19 suggesting the evolution from an ancestral gene through gene duplication and exon shuffling [4]. No mouse or rat ADGRE3 orthologues exist.

Full coding sequence human cDNA is publicly available, IMAGE:7211798 [5] in mammalian expression vector pCDNA3.1. Clone matches RefSeq and human reference genomic sequence.

References

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1. Bjarnadóttir TK, Fredriksson R, Höglund PJ, Gloriam DE, Lagerström MC, Schiöth HB. (2004) The human and mouse repertoire of the adhesion family of G-protein-coupled receptors. Genomics, 84: 23-33. [PMID:15203201]

2. Kane AJ, Sughrue ME, Rutkowski MJ, Phillips JJ, Parsa AT. (2010) EMR-3: a potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target. Neuroreport, 21 (16): 1018-22. [PMID:20827226]

3. Kwakkenbos MJ, Kop EN, Stacey M, Matmati M, Gordon S, Lin HH, Hamann J. (2004) The EGF-TM7 family: a postgenomic view. Immunogenetics, 55 (10): 655-66. [PMID:14647991]

4. Kwakkenbos MJ, Matmati M, Madsen O, Pouwels W, Wang Y, Bontrop RE, Heidt PJ, Hoek RM, Hamann J. (2006) An unusual mode of concerted evolution of the EGF-TM7 receptor chimera EMR2. FASEB J, 20: 2582-2584. [PMID:17068111]

5. Lennon G, Auffray C, Polymeropoulos M, Soares MB. (1996) The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression. Genomics, 33: 151-152. [PMID:8617505]

6. Matmati M, Pouwels W, van Bruggen R, Jansen M, Hoek RM, Verhoeven AJ, Hamann J. (2007) The human EGF-TM7 receptor EMR3 is a marker for mature granulocytes. J. Leukoc. Biol., 81 (2): 440-8. [PMID:17108056]

7. Plager DA, Kahl JC, Asmann YW, Nilson AE, Pallanch JF, Friedman O, Kita H. (2010) Gene transcription changes in asthmatic chronic rhinosinusitis with nasal polyps and comparison to those in atopic dermatitis. PLoS ONE, 5 (7): e11450. [PMID:20625511]

8. Stacey M, Lin HH, Hilyard KL, Gordon S, McKnight AJ. (2001) Human epidermal growth factor (EGF) module-containing mucin-like hormone receptor 3 is a new member of the EGF-TM7 family that recognizes a ligand on human macrophages and activated neutrophils. J Biol Chem, 276: 18863-18870. [PMID:11279179]

Contributors

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How to cite this page

Martin Stacey, Jörg Hamann, Hsi-Hsien Lin, Gabriela Aust, Tom I. Bonner.
Adhesion Class GPCRs: ADGRE3. Last modified on 03/03/2017. Accessed on 19/11/2018. IUPHAR/BPS Guide to PHARMACOLOGY, http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=184.