TRPC7 | Transient Receptor Potential channels | IUPHAR/BPS Guide to PHARMACOLOGY

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TRPC7

Target id: 492

Nomenclature: TRPC7

Family: Transient Receptor Potential channels

Annotation status:  image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates.  » Email us

   GtoImmuPdb view: OFF :     TRPC7 has curated data in GtoImmuPdb

Gene and Protein Information
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 1 862 5q31.2 TRPC7 transient receptor potential cation channel subfamily C member 7 11
Mouse 6 1 862 13 B2 Trpc7 transient receptor potential cation channel, subfamily C, member 7 8
Rat 6 1 861 17p14 Trpc7 transient receptor potential cation channel, subfamily C, member 7 9
Previous and Unofficial Names
TRP7 [8,11] | capacitative calcium channel TRPC7 | short transient receptor potential channel 7 | transient receptor potential cation channel
Database Links
CATH/Gene3D
Ensembl Gene
Entrez Gene
Human Protein Atlas
KEGG Gene
RefSeq Nucleotide
RefSeq Protein
UniProtKB
Wikipedia
Associated Proteins
Heteromeric Pore-forming Subunits
Name References
TRPC1 18
TRPC3 1
TRPC6 1
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
MxA 6
calmodulin 14
FK506 binding protein 1A 15
C-terminal tail-truncated TRPP2 7
Functional Characteristics
γ = 25–75 pS; conducts mono and divalent cations with a preference for divalents (PCa/ PCs = 5.9); modest outward rectification (monovalent cation current recorded in the absence of extracellular divalents); monovalent cation current suppressed by extracellular Ca2+ and Mg2+
Ion Selectivity and Conductance
Species:  Mouse
Rank order:  Ba2+ > Ca2+ > Na+ [25.0 - 50.0 pS] = Cs+
References:  14
Chemical activators (Human)
diacylglycerols

Download all structure-activity data for this target as a CSV file

Activators
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Affinity Units Concentration range (M) Holding voltage (mV) Reference
DOG Mm Agonist - - 1x10-4 -60.0 8
Conc range: 1x10-4 M [8]
Holding voltage: -60.0 mV
OAG Mm Agonist - - 1x10-4 -60.0 8
Conc range: 1x10-4 M [8]
Holding voltage: -60.0 mV
Channel Blockers
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Affinity Units Concentration range (M) Holding voltage (mV) Reference
La3+ Mm Antagonist - - 1x10-4 -60.0 8
Conc range: 1x10-4 M [8]
Holding voltage: -60.0 mV
SKF96365 Mm Antagonist - - 2.5x10-5 -60.0 8
Conc range: 2.5x10-5 M [8]
Holding voltage: -60.0 mV
SH045 Hs - 6.7 pIC50 - - 3
pIC50 6.7 (IC50 2.2x10-7 M) [3]
Description: Measuring inhibition of calcium flux in HEK293 cells expressing hTRPC7, in a FLIPR assay.
amiloride Hs - - - - -
2-APB Hs - - - - -
View species-specific channel blocker tables
Immunopharmacology Comments
Expressed on chicken B cells [10].
Tissue Distribution
Kidney, intestine, pituitary gland, brain.
Species:  Human
Technique:  RT-PCR
References:  12
Heart, lung eye, brain, kidney, liver, testis, retina, iris, inner ear
Species:  Mouse
Technique:  RT-PCR, western blotting, immunohistochemistry
References:  4,8,16-17
Brain
Species:  Rat
Technique:  RT-PCR
References:  2
Functional Assays
Calcium imaging.
Species:  Rat
Tissue:  Myocardium.
Response measured:  Intracellular Ca2+ levels.
References:  13
TRPC7 is constitutively activated and enhanced by stimulation of G protein-coupled receptor.
Species:  Mouse
Tissue: 
Response measured:  Intracellular Ca2+ levels.
References:  8
TRPC7 is activated by depletion of intracellular calcium stores.
Species:  Human
Tissue: 
Response measured:  Intracellular Ca2+ levels.
References:  11
Physiological Functions
The broad physiological role of TRPC7 is not known. TRPC7 conducts Ca2+ in AT1-induced myocardial apoptosis via a calcineurin-dependent pathway.
Species:  Rat
Tissue:  Myocardium
References:  13
In mouse, ablating the expression of both TRPC6 and TRPC7 eliminates the intrinsic light response of the M1 subtype of melanopsin-expressing, intrinsically photosensitive retinal ganglion cells (M1-ipRGCs).
Species:  Mouse
Tissue:  Eye
References:  17
Knock out of TRPC7 in DT40 B-lymphocytes alters thapsigargin activated [Ca2+]i signals.
Species:  Chicken
Tissue:  Immortalized chicken B-lymphocyte cell line DT40
References:  5

References

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1. Dietrich A, Kalwa H, Rost BR, Gudermann T. (2005) The diacylgylcerol-sensitive TRPC3/6/7 subfamily of cation channels: functional characterization and physiological relevance. Pflugers Arch., 451 (1): 72-80. [PMID:15971081]

2. Facemire CS, Mohler PJ, Arendshorst WJ. (2004) Expression and relative abundance of short transient receptor potential channels in the rat renal microcirculation. Am. J. Physiol. Renal Physiol., 286 (3): F546-51. [PMID:14678949]

3. Häfner S, Burg F, Kannler M, Urban N, Mayer P, Dietrich A, Trauner D, Broichhagen J, Schaefer M. (2018) A (+)-Larixol Congener with High Affinity and Subtype Selectivity toward TRPC6. ChemMedChem, 13 (10): 1028-1035. [PMID:29522264]

4. Jang Y, Lee Y, Kim SM, Yang YD, Jung J, Oh U. (2012) Quantitative analysis of TRP channel genes in mouse organs. Arch. Pharm. Res., 35 (10): 1823-30. [PMID:23139135]

5. Lievremont JP, Numaga T, Vazquez G, Lemonnier L, Hara Y, Mori E, Trebak M, Moss SE, Bird GS, Mori Y et al.. (2005) The role of canonical transient receptor potential 7 in B-cell receptor-activated channels. J. Biol. Chem., 280 (42): 35346-51. [PMID:16123040]

6. Lussier MP, Cayouette S, Lepage PK, Bernier CL, Francoeur N, St-Hilaire M, Pinard M, Boulay G. (2005) MxA, a member of the dynamin superfamily, interacts with the ankyrin-like repeat domain of TRPC. J. Biol. Chem., 280 (19): 19393-400. [PMID:15757897]

7. Miyagi K, Kiyonaka S, Yamada K, Miki T, Mori E, Kato K, Numata T, Sawaguchi Y, Numaga T, Kimura T et al.. (2009) A pathogenic C terminus-truncated polycystin-2 mutant enhances receptor-activated Ca2+ entry via association with TRPC3 and TRPC7. J. Biol. Chem., 284 (49): 34400-12. [PMID:19812035]

8. Okada T, Inoue R, Yamazaki K, Maeda A, Kurosaki T, Yamakuni T, Tanaka I, Shimizu S, Ikenaka K, Imoto K, Mori Y. (1999) Molecular and functional characterization of a novel mouse transient receptor potential protein homologue TRP7. Ca(2+)-permeable cation channel that is constitutively activated and enhanced by stimulation of G protein-coupled receptor. The Journal of biological chemistry, 274 (39): 27359-70. [PMID:10488066]

9. Onohara N, Nishida M, Inoue R, Kobayashi H, Sumimoto H, Sato Y, Mori Y, Nagao T, Kurose H. (2006) TRPC3 and TRPC6 are essential for angiotensin II-induced cardiac hypertrophy. The EMBO journal, 25 (22): 5305-16. [PMID:17082763]

10. Parenti A, De Logu F, Geppetti P, Benemei S. (2016) What is the evidence for the role of TRP channels in inflammatory and immune cells?. Br. J. Pharmacol., 173 (6): 953-69. [PMID:26603538]

11. Riccio A, Mattei C, Kelsell RE, Medhurst AD, Calver AR, Randall AD, Davis JB, Benham CD, Pangalos MN. (2002) Cloning and functional expression of human short TRP7, a candidate protein for store-operated Ca2+ influx. J. Biol. Chem., 277 (14): 12302-9. [PMID:11805119]

12. Riccio A, Medhurst AD, Mattei C, Kelsell RE, Calver AR, Randall AD, Benham CD, Pangalos MN. (2002) mRNA distribution analysis of human TRPC family in CNS and peripheral tissues. Brain Res. Mol. Brain Res., 109 (1-2): 95-104. [PMID:12531519]

13. Satoh S, Tanaka H, Ueda Y, Oyama J, Sugano M, Sumimoto H, Mori Y, Makino N. (2007) Transient receptor potential (TRP) protein 7 acts as a G protein-activated Ca2+ channel mediating angiotensin II-induced myocardial apoptosis. Mol. Cell. Biochem., 294 (1-2): 205-15. [PMID:16838106]

14. Shi J, Mori E, Mori Y, Mori M, Li J, Ito Y, Inoue R. (2004) Multiple regulation by calcium of murine homologues of transient receptor potential proteins TRPC6 and TRPC7 expressed in HEK293 cells. J. Physiol. (Lond.), 561 (Pt 2): 415-32. [PMID:15579537]

15. Sinkins WG, Goel M, Estacion M, Schilling WP. (2004) Association of immunophilins with mammalian TRPC channels. J. Biol. Chem., 279 (33): 34521-9. [PMID:15199065]

16. Takumida M, Anniko M. (2009) Expression of canonical transient receptor potential channel (TRPC) 1-7 in the mouse inner ear. Acta Otolaryngol., 129 (12): 1351-8. [PMID:19922081]

17. Xue T, Do MT, Riccio A, Jiang Z, Hsieh J, Wang HC, Merbs SL, Welsbie DS, Yoshioka T, Weissgerber P et al.. (2011) Melanopsin signalling in mammalian iris and retina. Nature, 479 (7371): 67-73. [PMID:22051675]

18. Zagranichnaya TK, Wu X, Villereal ML. (2005) Endogenous TRPC1, TRPC3, and TRPC7 proteins combine to form native store-operated channels in HEK-293 cells. J. Biol. Chem., 280 (33): 29559-69. [PMID:15972814]

Contributors

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How to cite this page

Antonio Riccio, David E. Clapham.
Transient Receptor Potential channels: TRPC7. Last modified on 01/06/2018. Accessed on 21/11/2018. IUPHAR/BPS Guide to PHARMACOLOGY, http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=492.