GPR32

Target id: 99

Nomenclature: GPR32

Family: Class A Orphans

Annotation status:  image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates.  » Email us

   GtoImmuPdb view: OFF :     Currently no data for GPR32 in GtoImmuPdb

Gene and Protein Information
class A G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 356 19q13.3 GPR32 G protein-coupled receptor 32
Previous and Unofficial Names
RvD1 | RVDR1 | resolvin D1 receptor
Database Links
Specialist databases
GPCRDB gpr32_human (Hs)
Other databases
Ensembl Gene
Entrez Gene
Human Protein Atlas
KEGG Gene
OMIM
RefSeq Nucleotide
RefSeq Protein
UniProtKB
Wikipedia
Natural/Endogenous Ligands
resolvin D1
Comments: Proposed ligand, single publication
Potency order of endogenous ligands
resolvin D1 > LXA4

Download all structure-activity data for this target as a CSV file

Agonists
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Affinity Units Reference
[3H]resolvin D1 Hs Agonist 9.7 pKd 3
pKd 9.7 (Kd 2x10-10 M) [3]
RvD1-ME Hs Full agonist 11.3 pEC50 2
pEC50 11.3 (EC50 4.6x10-12 M) [2]
resolvin D1 Hs Full agonist 11.1 pEC50 3
pEC50 11.1 (EC50 8.8x10-12 M) [3]
LXA4 Hs Full agonist 9.7 pEC50 3
pEC50 9.7 (EC50 2x10-10 M) [3]
Agonist Comments
Resolvin D1 has been demonstrated to activate GPR32 in a single publication [3]. This result is supported by the findings of Chiang et al. which demonstrate that Resolvin D1 inhibits TNFalpha mediated NFκB activation via GPR32 [1]. The pairing was not replicated in a recent studty based on β-arrestin recruitment [6].
Tissue Distribution
Arterial and venous tissue
Species:  Human
Technique:  Microarray analysis
References:  3
Myeloid cells, human umbilical vein cells
Species:  Human
Technique:  RT-PCR
References:  3
Tissue Distribution Comments
Surface expression in human monocytes up-regulated by zymosan and G-CSF [3].
Physiological Consequences of Altering Gene Expression
Overexpression of GPR32 enhances RvD1 mediated macrophage phagocytosis of human polymorphonuclear leukocytes
Species:  Human
Tissue:  Macrophage
Technique:  RNA interference
References:  3
Selective knockdown decreases RvD1 mediate macrophage phagocytosis of human polymorphonuclear leukocytes
Species:  Human
Tissue:  Macrophage
Technique:  RNA interference
References:  3
Macrophages overexpressing GPR32 show significantly upregulated miRNA-21, and downregulation of miR-302d and miR-219
Species:  Mouse
Tissue:  Macrophage
Technique:  Gene overexpression
References:  5
Biologically Significant Variants
Type:  Single nucleotide polymorphism
Species:  Human
Amino acid change:  F327L
Global MAF (%):  2
Subpopulation MAF (%):  AMR|ASN|EUR: 3|2|3
Minor allele count:  C=0.021/47
Comment on frequency:  Low frequency (<10% in all tested populations)
SNP accession: 
Validation:  1000 Genomes, Frequency
Type:  Single nucleotide polymorphism
Species:  Human
Amino acid change:  P354S
Global MAF (%):  1
Subpopulation MAF (%):  AFR: 2
Minor allele count:  T=0.005/11
Comment on frequency:  Low frequency (<10% in all tested populations)
SNP accession: 
Validation:  Frequency
General Comments
GPR32 is a pseudogene in mice and rats [3].

Resolvin mediated activation of GPR32 may be important in the resolution of inflammatory disease [4].

References

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1. Chiang N, Fredman G, Bäckhed F, Oh SF, Vickery T, Schmidt BA, Serhan CN. (2012) Infection regulates pro-resolving mediators that lower antibiotic requirements. Nature484 (7395): 524-8. [PMID:22538616]

2. Krishnamoorthy S, Recchiuti A, Chiang N, Fredman G, Serhan CN. (2012) Resolvin D1 receptor stereoselectivity and regulation of inflammation and proresolving microRNAs. Am. J. Pathol.180 (5): 2018-27. [PMID:22449948]

3. Krishnamoorthy S, Recchiuti A, Chiang N, Yacoubian S, Lee CH, Yang R, Petasis NA, Serhan CN. (2010) Resolvin D1 binds human phagocytes with evidence for proresolving receptors. Proc. Natl. Acad. Sci. U.S.A.107 (4): 1660-5. [PMID:20080636]

4. Lee CH. (2012) Resolvins as new fascinating drug candidates for inflammatory diseases. Arch. Pharm. Res.35 (1): 3-7. [PMID:22297737]

5. Pappo AS, Krailo M, Chen Z, Rodriguez-Galindo C, Reaman G. (2010) Infrequent tumor initiative of the Children's Oncology Group: initial lessons learned and their impact on future plans. J. Clin. Oncol.28 (33): 5011-6. [PMID:20956621]

6. Southern C, Cook JM, Neetoo-Isseljee Z, Taylor DL, Kettleborough CA, Merritt A, Bassoni DL, Raab WJ, Quinn E, Wehrman TS et al.. (2013) Screening β-Arrestin Recruitment for the Identification of Natural Ligands for Orphan G-Protein-Coupled Receptors. J Biomol Screen18 (5): 599-609. [PMID:23396314]

Contributors

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How to cite this page

Anthony P. Davenport, Stephen Alexander, Joanna L. Sharman, Adam J. Pawson, Helen E. Benson, Amy E. Monaghan, Wen Chiy Liew, Chido Mpamhanga, Jim Battey, Richard V. Benya, Robert T. Jensen, Sadashiva Karnik, Evi Kostenis, Eliot Spindel, Laura Storjohann, Kalyan Tirupula, Tom I. Bonner, Richard Neubig, Jean-Philippe Pin, Michael Spedding, Anthony Harmar.
Class A Orphans: GPR32. Last modified on 31/07/2015. Accessed on 21/10/2017. IUPHAR/BPS Guide to PHARMACOLOGY, http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=99.