MMV008138 [Ligand Id: 10229] activity data from GtoPdb and ChEMBL
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| ChEMBL ligand: CHEMBL527593 (GNF-Pf-4188, MMV008138, TCMDC-125815) |
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| DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
|---|---|---|---|---|---|---|---|---|
| Calcium-dependent protein kinase 1 in Plasmodium falciparum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1908387] [UniProtKB: P62344] | ||||||||
| ChEMBL | MMV: CDPK1 Protein kinase inhibition assay using recombinant PfCDPK1, syntide 2 peptide substrate, ATP and KinaseGlo to measure % inhibition at 1 uM MMV box compound. | F | 6 | pIC50 | >1000 | nM | IC50 | MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases. |
| Calcium-dependent protein kinase 4 in Plasmodium falciparum 3D7 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2169725] [UniProtKB: Q8IBS5] | ||||||||
| ChEMBL | MMV: CDPK4 Protein kinase inhibition assay using recombinant PfCDPK4, syntide2 peptide substrate, ATP and KinaseGlo to measure % inhibition at 1 uM MMV box compound. | F | 6 | pIC50 | >1000 | nM | IC50 | MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases. |
| Cdk-related protein kinase 6 in Plasmodium falciparum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3301558] [UniProtKB: O77239] | ||||||||
| ChEMBL | MMV: PK6 Protein kinase inhibition assay using recombinant PfPK6, Myelin Basic Protein (MBP) substrate, ATP and KinaseGlo to measure % inhibition at 1 uM MMV box compound. Final concentrations were 1.5 uM ATP, 5 mM MnCl2, and 15 ug/ml PK6 in a buffer of 100 mM Tris-HCl (pH 7.5). 50 ug/mL MBP was provided as the substrate; 10 uM staurosporine was used as a control inhibitor. Incubation time was 3 hours and 40 minutes.The catalytic activity of each kinase was considered proportional to ATP consumed, as determined from measurements of residual [ATP] with the luciferase-based reagent Kinase-Glo (Promega) following incubation. Luminescence (proportional to residual [ATP]) was measured on the plate readers FLx800 (BioTek Instruments, Winooski, VT, USA) and MicroBeta2. | F | 6 | pIC50 | >1000 | nM | IC50 | MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases. |
| Glucose transporter in Leishmania mexicana (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3431938] [UniProtKB: O61059] | ||||||||
| ChEMBL | ST_JUDE_LEISH: Cytotoxicity against transgenic Leishmania Mexicana promastigotes LmGT2 that are glucose transport deficient and complemented with the L. Mexicana glucose transporter 2. Activity is measured by by DNA content using SYBR green in vitro | F | 4.92 | pIC50 | >12000 | nM | IC50 | St. Jude Leishmania screening dataset. |
| Glucose transporter 1/Glucose transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2535] [GtoPdb: 875] [UniProtKB: P11166] | ||||||||
| ChEMBL | ST_JUDE_LEISH: Cytotoxicity against transgenic Leishmania Mexicana promastigotes LmGLUT1 that are glucose transport deficient and complemented with the human glucose transporter GLUT1. Activity is measured by DNA content using SYBR green in vitro | F | 4.92 | pIC50 | >12000 | nM | IC50 | St. Jude Leishmania screening dataset. |
| Hexose transporter 1 in Plasmodium falciparum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4697] [UniProtKB: O97467] | ||||||||
| ChEMBL | ST_JUDE_LEISH: Cytotoxicity against transgenic Leishmania Mexicana promastigotes LmPfHT that are glucose transport deficient and complemented with the Plasmodium falciparum hexose transporter. Activity is measured by by DNA content using SYBR green in vitro | F | 4.92 | pIC50 | >12000 | nM | IC50 | St. Jude Leishmania screening dataset. |
| Plasmodium falciparum lysyl-tRNA synthetase/Lysine--tRNA ligase in Plasmodium falciparum 3D7 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3301561] [GtoPdb: 3059] [UniProtKB: Q8IDJ8] | ||||||||
| ChEMBL | MMV: KRS recombinant Plasmodium falciparum Lysyl-tRNA-synthetase, assay with yeast tRNA, ATP, and lysine and 3 uM MMV malaria box compound, assay read out with kinase glo as % inhibition. | F | 5.6 | pIC50 | >2500 | nM | IC50 | MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases. |
| Mitogen-activated protein kinase in Plasmodium falciparum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3301559] [UniProtKB: Q25917] | ||||||||
| ChEMBL | MMV: MAP2K Protein kinase inhibition assay using recombinant PfMAP2K, protein substrate, ATP and KinaseGlo to measure % inhibition at 1 uM MMV box compound. Final concentrations were 1 uM ATP, 0.5 mM DTT, 1 mM MgCl2, 0.5 mg/mL BSA, and 10 ug/ml MAP2 in a buffer of 50 mM HEPES (pH 7.0). 0.5 mg/mL histone III-S served as the substrate (13); 100 uM AMP-PNP, an ATP analog, was used as a control inhibitor. Incubation time was 4 hours. | F | 6 | pIC50 | >1000 | nM | IC50 | MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases. |
| Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364] | ||||||||
| ChEMBL | MMV: 3H-Hyoxanthine uptake assay; RPMI 1640 (Invitrogen cat#11875-093) with Sodium Bicarbonate (NaHCO3) 2000 mg/mL. | F | 6 | pIC50 | >1000 | nM | IC50 | MMV Mararia Box screening against P. falciparum asexual stage parasites under hypoxia and bicarbonate modified culture conditions. |
| ChEMBL | MMV: Modified 3H-Hyoxanthine uptake assay; RPMI 1640 (Invitrogen cat#23400-013) without Sodium Bicarbonate (NaHCO3). | F | 6 | pIC50 | >1000 | nM | IC50 | MMV Mararia Box screening against P. falciparum asexual stage parasites under hypoxia and bicarbonate modified culture conditions. |
| ChEMBL | MMV: 3H-Hyoxanthine uptake assay; 5% O2, 5% CO2 in N2. | F | 6 | pIC50 | >1000 | nM | IC50 | MMV Mararia Box screening against P. falciparum asexual stage parasites under hypoxia and bicarbonate modified culture conditions. |
| ChEMBL | MMV: Modified 3H-Hyoxanthine uptake assay; 1% O2, 5% CO2 in N2. | F | 6 | pIC50 | >1000 | nM | IC50 | MMV Mararia Box screening against P. falciparum asexual stage parasites under hypoxia and bicarbonate modified culture conditions. |
| ChEMBL | NOVARTIS: Inhibition of Plasmodium falciparum 3D7 (drug-susceptible) proliferation in erythrocyte-based infection assay | F | 5.99 | pEC50 | 1023 | nM | EC50 | Proc Natl Acad Sci U S A (2008) 105: 9059-9064 [PMID:18579783] |
| ChEMBL | NOVARTIS: Inhibition of Plasmodium falciparum W2 (drug-resistant) proliferation in erythrocyte-based infection assay | F | 6.16 | pEC50 | 688 | nM | EC50 | Proc Natl Acad Sci U S A (2008) 105: 9059-9064 [PMID:18579783] |
| ChEMBL | MMV: Inhibition of Plasmodium falciparum 3D7 (EC50). | F | 6.46 | pEC50 | 350 | nM | EC50 | Malaria Box |
| Plasmodium falciparum prolyl-tRNA synthetase/Proline--tRNA ligase in Plasmodium falciparum 3D7 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3301560] [GtoPdb: 3056] [UniProtKB: Q8I5R7] | ||||||||
| ChEMBL | MMV: ProRS recombinant Plasmodium falciparum Prolyl-tRNA-synthetase, assay with yeast tRNA, ATP, and proline and 3 uM MMV malaria box compound, assay read out with kinase glo as % inhibition. | F | 5.6 | pIC50 | >2500 | nM | IC50 | MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases. |
| Putative uncharacterized protein pk7 in Plasmodium falciparum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6169] [UniProtKB: O96214] | ||||||||
| ChEMBL | MMV: PK7 Protein kinase inhibition assay using recombinant PfPK7, ATP and KinaseGlo to measure % inhibition at 1 uM MMV box compound. Final concentrations were 1 uM ATP, 2 mM DTT, 20 mM MgCl2, 2 mM MnCl2, 0.01% BSA, and 6 ug/ml PK7, in a buffer of 20 mM Tris-HCl (pH 7.5). The enzyme itself was the only substrate present (since autophosphorylation occurs); 100 uM 1NA-PP1 was used as a control inhibitor. Incubation time was 3 hours. | F | 6 | pIC50 | >1000 | nM | IC50 | MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases. |
| Plasmodium falciparum 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase in Plasmodium falciparum W2 [GtoPdb: 3064] | ||||||||
| GtoPdb | Parasite growth inhibition assay | - | 6.96 | pEC50 | 110 | nM | EC50 | Antimicrob Agents Chemother (2015) 59: 356-64 [PMID:25367906] |
| Plasmodium falciparum 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase in Plasmodium falciparum [GtoPdb: 3064] | ||||||||
| GtoPdb | Enzymatic activity measured using a pyrophosphate release assay | - | 8.15 | pIC50 | 7 | nM | IC50 | Antimicrob Agents Chemother (2015) 59: 356-64 [PMID:25367906] |
| Plasmodium falciparum 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase in Plasmodium falciparum Dd2 [GtoPdb: 3064] | ||||||||
| GtoPdb | Parasite growth inhibition assay | - | 6.6 | pIC50 | 250 | nM | IC50 | Bioorg Med Chem Lett (2015) 25: 1515-9 [PMID:25754494] |
ChEMBL data shown on this page come from version 33:
Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]
