ChEMBL ligand: CHEMBL36506 (Albamycin, Albamycin t, Cathomycin, Novobiocin, Novobiocina, Novobiocine)
ABCG2/ATP-binding cassette sub-family G member 2 in Human [ChEMBL: CHEMBL5393] [GtoPdb: 792] [UniProtKB: Q9UNQ0] There should be some charts here, you may need to enable JavaScript!
DNA gyrase subunit A/DNA gyrase subunit B/DNA gyrase in Escherichia coli [ChEMBL: CHEMBL2094139] [GtoPdb: 3217, 3218] [UniProtKB: P0AES4, P0AES6] DNA gyrase in S.aureus [ChEMBL: CHEMBL3038482] [UniProtKB: P0A0K8, P20831] DNA gyrase subunit A/DNA gyrase subunit B in Escherichia coli [GtoPdb: 3217, 3218] [UniProtKB: P0AES4, P0AES6] There should be some charts here, you may need to enable JavaScript!
DNA gyrase subunit A/DNA gyrase subunit B in Mycobacterium tuberculosis [ChEMBL: CHEMBL3430898] [UniProtKB: P9WG45, P9WG47] There should be some charts here, you may need to enable JavaScript!
DNA gyrase subunit B in Escherichia coli [ChEMBL: CHEMBL1826] [GtoPdb: 3218] [UniProtKB: P0AES6] DNA gyrase subunit B in S.aureus [ChEMBL: CHEMBL1921666] [UniProtKB: P0A0K8] DNA gyrase subunit B in Mycobacterium smegmatis [ChEMBL: CHEMBL3085613] [UniProtKB: P0C559] DNA gyrase subunit B in Escherichia coli [GtoPdb: 3218] [UniProtKB: P0AES6] DNA gyrase subunit B in Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) [ChEMBL: CHEMBL1075021] [UniProtKB: P0A4L9] DNA gyrase subunit B in Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [ChEMBL: CHEMBL6059] [UniProtKB: A0QNE0] There should be some charts here, you may need to enable JavaScript!
DNA polymerase theta in Human [ChEMBL: CHEMBL6025] [GtoPdb: 3230] [UniProtKB: O75417] There should be some charts here, you may need to enable JavaScript!
DNA topoisomerase 4 subunit B in S.aureus [ChEMBL: CHEMBL2150841] [UniProtKB: P0C1S7] There should be some charts here, you may need to enable JavaScript!
Genome polyprotein in Zika virus [ChEMBL: CHEMBL4523954] [UniProtKB: A0A024AXB9] There should be some charts here, you may need to enable JavaScript!
Microtubule-associated proteins 1A/1B light chain 3A in Human [ChEMBL: CHEMBL4879518] [UniProtKB: Q9H492] There should be some charts here, you may need to enable JavaScript!
Microtubule-associated proteins 1A/1B light chain 3B in Human [ChEMBL: CHEMBL4879514] [UniProtKB: Q9GZQ8] There should be some charts here, you may need to enable JavaScript!
ParE in Streptococcus pneumoniae [ChEMBL: CHEMBL1075022] [UniProtKB: Q7X1P7] There should be some charts here, you may need to enable JavaScript!
Topoisomerase IV in S.aureus [ChEMBL: CHEMBL3038508] [UniProtKB: P0C1S7, P0C1U9] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
ABCG2/ATP-binding cassette sub-family G member 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5393] [GtoPdb: 792] [UniProtKB: Q9UNQ0]
ChEMBL	Inhibition of BCRP expressed in MCF7 MX cells by Hoechst 33342 staining	B	4.07	pIC50	85100	nM	IC50	Bioorg Med Chem Lett (2010) 20: 180-183 [PMID:19932960]
ChEMBL	Inhibition of ABCG2 in human mitoxantrone-resistant MCF7 cells by Hoechst 33342 assay	B	4.18	pIC50	66069.34	nM	IC50	Bioorg Med Chem (2008) 16: 8224-8236 [PMID:18678495]
ChEMBL	Inhibition of ABCG2 (unknown origin) expressed human PC-6/SN2-5 cells membrane vesicle mediated topotecan transport by HPLC analysis	B	4.57	pIC50	26800	nM	IC50	Eur J Med Chem (2022) 237: 114346-114346 [PMID:35483322]
ChEMBL	TP_TRANSPORTER: inhibition of mitoxantrone efflux in BCRP-expressing MCF7-MX cells	F	4.6	pIC50	25000	nM	IC50	Placenta (2006) 27: 1096-1102 [PMID:16460798]
DNA gyrase subunit A/DNA gyrase subunit B/DNA gyrase in Escherichia coli (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2094139] [GtoPdb: 3217, 3218] [UniProtKB: P0AES4, P0AES6]
ChEMBL	Inhibition of Escherichia coli DNA gyrase supercoiling activity	B	6.3	pIC50	500	nM	IC50	Bioorg Med Chem (2016) 24: 6291-6297 [PMID:27143131]
ChEMBL	Inhibition of Escherichia coli DNA gyrase ATPase activity using DNA as substrate after 60 mins	B	6.61	pIC50	245	nM	IC50	Medchemcomm (2017) 8: 942-951 [PMID:30034678]
ChEMBL	Inhibition of Escherichia coli DNA gyrase using biotinylated oligonucleotide as substrate incubated for 30 mins in presence of ATP by fluorescence-based microplate reader assay	B	6.77	pIC50	170	nM	IC50	Eur J Med Chem (2019) 167: 269-290 [PMID:30776691]
ChEMBL	Inhibition of Escherichia coli DNA gyrase using relaxed pNO1 plasmid and biotinylated oligonucelotide incubated for 30 mins by fluorescence based DNA super-coiling assay	B	6.77	pIC50	170	nM	IC50	J Med Chem (2015) 58: 5501-5521 [PMID:26098163]
ChEMBL	Inhibition of Escherichia coli DNA gyrase assessed as reduction in relaxation of supercoiled pNO1 DNA measured after 30 mins by fluorescence assay	B	6.77	pIC50	170	nM	IC50	Eur J Med Chem (2021) 213: 113200-113200 [PMID:33524686]
ChEMBL	Inhibition of Escherichia coli DNA gyrase using relaxed pNO1 plasmid DNA as substrate incubated for 30 mins by fluorescence assay	B	6.77	pIC50	170	nM	IC50	Medchemcomm (2019) 10: 1007-1017 [PMID:31303999]
ChEMBL	Inhibition of Escherichia coli DNA gyrase using relaxed pNO1 plasmid DNA as substrate incubated for 30 mins by fluorescence assay	B	6.77	pIC50	168	nM	IC50	ACS Med Chem Lett (2020) 11: 2433-2440 [PMID:33329764]
ChEMBL	Inhibition of Escherichia coli DNA gyrase assessed as inhibition of pUC19 supercoiling after 30 mins by agarose gel electrophoresis	B	6.84	pIC50	144	nM	IC50	Medchemcomm (2017) 8: 942-951 [PMID:30034678]
ChEMBL	Inhibition of reconstituted Escherichia coli DNA gyrase subunit AB assessed as reduction of DNA supercoiling activity using pBR322 DNA substrate	B	7	pIC50	100	nM	IC50	Antimicrob Agents Chemother (2008) 52: 1382-1390 [PMID:18268084]
ChEMBL	Inhibition of Escherichia coli DNA gyrase using relaxed pBR322 DNA as substrate after 3 hrs by DNA super-coiling assay	B	7.1	pIC50	80	nM	IC50	J Med Chem (2015) 58: 5501-5521 [PMID:26098163]
GtoPdb	DNA gyrase supercoiling assay	-	7.1	pIC50	80	nM	IC50	J Antimicrob Chemother (2011) 66: 2061-9 [PMID:21693461]
ChEMBL	Inhibition of Escherichia coli DNA gyrase by measuring supercoiling activity incubated for 60 mins by fluorescence based assay	B	7.62	pIC50	23.88	nM	IC50	Bioorg Med Chem (2020) 28: 115776-115776 [PMID:33032189]
ChEMBL	Inhibition of Escherichia coli DNA gyrase ATPase activity	B	7.64	pIC50	23	nM	IC50	Bioorg Med Chem (2016) 24: 6291-6297 [PMID:27143131]
ChEMBL	Inhibition of Escherichia coli DNA gyrase	B	8.34	pIC50	4.52	nM	IC50	RSC Med Chem (2022) 13: 522-567 [PMID:35694689]
DNA gyrase in S.aureus (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL3038482] [UniProtKB: P0A0K8, P20831]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase	B	8	pKi	10	nM	Ki	J Med Chem (2008) 51: 5243-5263 [PMID:18690678]
ChEMBL	Inhibition of Staphylococcus aureus N-terminal His6-tagged DNA gyrase subunit GyrA/GyrB supercoiling activity expressed in Escherichia coli BL21 (DE3) using relaxed pBR322 DNA as substrate after 1 hr by agarose gel electrophoresis	B	6.9	pIC50	125	nM	IC50	J Med Chem (2017) 60: 3755-3775 [PMID:28406299]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase using relaxed pNO1 plasmid DNA as substrate incubated for 30 mins by fluorescence assay	B	7.39	pIC50	41	nM	IC50	ACS Med Chem Lett (2020) 11: 2433-2440 [PMID:33329764]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase assessed as supercoiling of relaxed pNO1 plasmid after 30 mins by SybrGOLD staining based fluorescence assay	B	7.39	pIC50	41	nM	IC50	Eur J Med Chem (2016) 117: 197-211 [PMID:27100032]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase A/DNA gyrase B supercoiling activity using relaxed pNO1 DNA as substrate after 30 mins in presence of biotin-labeled oligonucleotide by SybrGOLD staining based fluorescence assay	B	7.39	pIC50	41	nM	IC50	Eur J Med Chem (2017) 130: 171-184 [PMID:28246042]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase using relaxed pNO1 plasmid DNA as substrate incubated for 30 mins by fluorescence assay	B	7.39	pIC50	41	nM	IC50	Medchemcomm (2019) 10: 1007-1017 [PMID:31303999]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase using biotinylated oligonucleotide as substrate incubated for 30 mins in presence of ATP by fluorescence-based microplate reader assay	B	7.39	pIC50	41	nM	IC50	Eur J Med Chem (2019) 167: 269-290 [PMID:30776691]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase using relaxed pNO1 plasmid and biotinylated oligonucelotide incubated for 30 mins by fluorescence based DNA super-coiling assay	B	7.4	pIC50	40	nM	IC50	J Med Chem (2015) 58: 5501-5521 [PMID:26098163]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase supercoiling activity using relaxed pHOT-1 DNA as substrate after 60 mins by ethidium bromide-based gel electrophoresis	B	7.4	pIC50	40	nM	IC50	J Med Chem (2019) 62: 2950-2973 [PMID:30698430]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase assessed reduction in relaxation of supercoiled pNO1 DNA measured after 30 mins by fluorescence assay	B	7.47	pIC50	34	nM	IC50	Eur J Med Chem (2021) 213: 113200-113200 [PMID:33524686]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase-mediated supercoiling of relaxed DNA using pBR 322 as substrate by agarose gel electrophoresis analysis	B	7.52	pIC50	30	nM	IC50	Bioorg Med Chem (2014) 22: 5970-5987 [PMID:25288496]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase using relaxed pBR322 DNA as substrate after 3 hrs by DNA super-coiling assay	B	8	pIC50	10	nM	IC50	J Med Chem (2015) 58: 5501-5521 [PMID:26098163]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase assessed as supercoiling of relaxed pBR322 plasmid after 45 mins by ethidium bromide staining based agarose gel electrophoresis	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2016) 117: 197-211 [PMID:27100032]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase supercoiling activity	B	8.4	pIC50	<4	nM	IC50	Bioorg Med Chem (2016) 24: 6291-6297 [PMID:27143131]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase	B	8.4	pIC50	<4	nM	IC50	Bioorg Med Chem Lett (2012) 22: 7127-7130 [PMID:23084277]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase supercoiling incubated for 30 mins in presence of ATP by ethidium bromide staining based analysis	B	9.19	pIC50	0.65	nM	IC50	J Med Chem (2023) 66: 3968-3994 [PMID:36877255]
DNA gyrase subunit A/DNA gyrase subunit B in Escherichia coli [GtoPdb: 3217, 3218] [UniProtKB: P0AES4, P0AES6]
GtoPdb	DNA gyrase supercoiling assay	-	7.1	pIC50	80	nM	IC50	J Antimicrob Chemother (2011) 66: 2061-9 [PMID:21693461]
DNA gyrase subunit A/DNA gyrase subunit B in Mycobacterium tuberculosis (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL3430898] [UniProtKB: P9WG45, P9WG47]
ChEMBL	Inhibition of Mycobacterium tuberculosis DNA gyrase assessed as inhibition of supercoiling activity using relaxed pBR322 plasmid as substrate after 30 mins	B	4.34	pIC50	46000	nM	IC50	Bioorg Med Chem (2016) 24: 42-52 [PMID:26678175]
ChEMBL	Inhibition of Mycobacterium tuberculosis DNA gyrase activity	B	7.07	pIC50	85	nM	IC50	Bioorg Med Chem Lett (2018) 28: 2998-3003 [PMID:30122228]
ChEMBL	Inhibition of Mycobacterium tuberculosis DNA gyrase super coiling activity of relaxed pBR322 using ethidium bromide staining by agarose gel electrophoresis	B	7.3	pIC50	50	nM	IC50	Eur J Med Chem (2013) 70: 143-153 [PMID:24148991]
ChEMBL	Inhibition of Mycobacterium tuberculosis DNA gyrase using relaxed pBR322 as substrate after 30 mins by supercoiling assay	B	7.34	pIC50	46	nM	IC50	Bioorg Med Chem (2014) 22: 6552-6563 [PMID:25456076]
ChEMBL	Inhibition of Mycobacterium tuberculosis GyrA/GyrB using relaxed pBR322 substrate incubated for 30 mins by ethidium bromide staining and electrophoresis based DNA supercoiling assay	B	7.34	pIC50	46	nM	IC50	Bioorg Med Chem (2015) 23: 2062-2078 [PMID:25801151]
ChEMBL	Inhibition of DNA gyrase GyrA/GyrB in Mycobacterium tuberculosis incubated for 30 mins assessed as reduction in supercoiling of relaxed relaxed pBR322 DNA by ethidium bromide staining based agarose gel electrophoresis method	B	7.34	pIC50	46	nM	IC50	Eur J Med Chem (2015) 103: 1-16 [PMID:26318054]
ChEMBL	Inhibition of Mycobacterium tuberculosis DNA gyrase assessed as inhibition of DNA supercoiling activity using relaxed pBR322 as substrate incubated for 30 mins by ethidium bromide staining based agarose gel electrophoresis	B	7.34	pIC50	46	nM	IC50	Eur J Med Chem (2016) 124: 160-185 [PMID:27569197]
ChEMBL	Inhibition of Mycobacterium tuberculosis DNA gyrase supercoiling activity using relaxed pBR322 substrate incubated for 30 mins by ethidium bromide staining method	B	7.34	pIC50	46	nM	IC50	Bioorg Med Chem (2014) 22: 4924-4934 [PMID:25129171]
ChEMBL	Inhibition of Mycobacterium tuberculosis DNA gyrase assessed as inhibition of DNA supercoiling after 30 mins by electrophoresis	B	7.34	pIC50	46	nM	IC50	Bioorg Med Chem (2016) 24: 877-885 [PMID:26787274]
ChEMBL	Inhibition of Mycobacterium tuberculosis DNA gyrase ATPase activity	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2018) 28: 2998-3003 [PMID:30122228]
ChEMBL	Inhibition of Mycobacterium tuberculosis DNA gyrase-mediated relaxed pBR322 DNA supercoiling by gel electrophoresis	B	7.82	pIC50	15	nM	IC50	ACS Med Chem Lett (2014) 5: 1005-1009 [PMID:25221657]
DNA gyrase subunit B in Escherichia coli (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1826] [GtoPdb: 3218] [UniProtKB: P0AES6]
ChEMBL	Binding affinity to Escherichia coli GyrB	B	7.55	pKd	28	nM	Kd	J Med Chem (2011) 54: 915-929 [PMID:21235241]
ChEMBL	Binding affinity to Escherichia coli H560 DNA gyrase B	B	8.15	pKd	7	nM	Kd	Eur J Med Chem (2016) 124: 160-185 [PMID:27569197]
ChEMBL	Inhibition of Escherichia coli H560 DNA gyrase B ATPase activity after 30 mins by ammonium molybdate/malachite green-based phosphate detection assay	B	8.15	pKi	7	nM	Ki	Eur J Med Chem (2016) 124: 160-185 [PMID:27569197]
ChEMBL	Inhibition of Escherichia coli JM109 DNA gyrase subunit B assessed as DNA triple helix formation by supercoiling assay	B	6.7	pIC50	200	nM	IC50	Antimicrob Agents Chemother (2008) 52: 1982-1990 [PMID:18347114]
ChEMBL	Inhibition of Escherichia coli DNA gyrase B ATP binding site assessed as reduction in supercoiling of relaxed pNO1 DNA after 30 mins in presence of biotinylated oligonucleotide by sybrGOLD staining based fluorescence assay	B	6.77	pIC50	170	nM	IC50	J Med Chem (2016) 59: 8941-8954 [PMID:27541007]
ChEMBL	Inhibition of Escherichia coli ATCC 25922 DNA gyrase B using relaxed pNO1 DNA as substrate incubated for 30 mins and measured by sybrGOLD staining based fluorescence assay	B	6.77	pIC50	170	nM	IC50	Eur J Med Chem (2017) 125: 500-514 [PMID:27689732]
ChEMBL	Inhibition of Escherichia coli C-terminal His6-tagged DNA gyrase B expressed in Escherichia coli BL21(DE3) preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method	B	6.77	pIC50	170	nM	IC50	Eur J Med Chem (2018) 154: 117-132 [PMID:29778894]
ChEMBL	Inhibition of Escherichia coli JM109 DNA gyrase subunit B assessed as ATP hydrolysis by ATPase assay	B	6.81	pIC50	154	nM	IC50	Antimicrob Agents Chemother (2008) 52: 1982-1990 [PMID:18347114]
GtoPdb	DNA gyrase supercoiling assay	-	7.1	pIC50	80	nM	IC50	J Antimicrob Chemother (2011) 66: 2061-9 [PMID:21693461]
DNA gyrase subunit B in S.aureus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1921666] [UniProtKB: P0A0K8]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase B ATPase activity	B	6.9	pIC50	125	nM	IC50	Bioorg Med Chem (2014) 22: 5970-5987 [PMID:25288496]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase B using relaxed pNO1 DNA as substrate incubated for 30 mins and measured by sybrGOLD staining based fluorescence assay	B	7.39	pIC50	41	nM	IC50	Eur J Med Chem (2017) 125: 500-514 [PMID:27689732]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase B preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method	B	7.39	pIC50	41	nM	IC50	Eur J Med Chem (2018) 154: 117-132 [PMID:29778894]
ChEMBL	Inhibition of Staphylococcus aureus DNA gyrase B ATP binding site assessed as reduction in supercoiling of relaxed pNO1 DNA after 30 mins in presence of biotinylated oligonucleotide by sybrGOLD staining based fluorescence assay	B	7.4	pIC50	40	nM	IC50	J Med Chem (2016) 59: 8941-8954 [PMID:27541007]
DNA gyrase subunit B in Mycobacterium smegmatis (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3085613] [UniProtKB: P0C559]
ChEMBL	Inhibition of Mycobacterium smegmatis GyrB ATPase activity expressed in Escherichia coli BL21 (DE3) after 2 hrs	B	6.7	pIC50	200	nM	IC50	Eur J Med Chem (2013) 70: 143-153 [PMID:24148991]
ChEMBL	Inhibition of purified Mycobacterium smegmatis GyrB at 50 to 100 uM after 100 mins by ATPase assay	B	6.74	pIC50	180	nM	IC50	Bioorg Med Chem (2014) 22: 6552-6563 [PMID:25456076]
ChEMBL	Inhibition of 6-His-tagged Mycobacterium smegmatis GyrB expressed in Escherichia coli BL21 (DE3) pLysS cells incubated for 100 mins in presence of ATP by malachite green dye based ATP assay	B	6.74	pIC50	180	nM	IC50	Bioorg Med Chem (2014) 22: 4924-4934 [PMID:25129171]
ChEMBL	Inhibition of Mycobacterium smegmatis GyrB ATPase activity expressed in Escherichia coli BL21 (DE3) pLysS cells after 100 mins by inorganic phosphate release detection based malachite green reagent assay	B	6.74	pIC50	180	nM	IC50	Bioorg Med Chem (2015) 23: 2062-2078 [PMID:25801151]
ChEMBL	Inhibition of Mycobacterium smegmatis DNA gyrase B expressed in Escherichia coli BL21 (DE3) pLysS cells assessed as reduction in ATPase activity incubated for 100 mins by inorganic phosphate detection assay	B	6.74	pIC50	180	nM	IC50	Eur J Med Chem (2015) 103: 1-16 [PMID:26318054]
ChEMBL	Inhibition of Mycobacterium smegmatis DNA GyrB domain assessed as inhibition of inorganic phosphate release after 100 mins at room temperature by ATPase assay	B	7.34	pIC50	46	nM	IC50	Bioorg Med Chem (2015) 23: 1402-1412 [PMID:25766629]
DNA gyrase subunit B in Escherichia coli [GtoPdb: 3218] [UniProtKB: P0AES6]
GtoPdb	DNA gyrase supercoiling assay	-	7.1	pIC50	80	nM	IC50	J Antimicrob Chemother (2011) 66: 2061-9 [PMID:21693461]
DNA gyrase subunit B in Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075021] [UniProtKB: P0A4L9]
ChEMBL	Inhibition of Streptococcus pneumoniae SP-3 gyrB by enzyme coupled phosphate assay	B	7.43	pIC50	37	nM	IC50	Bioorg Med Chem Lett (2009) 19: 5302-5306 [PMID:19683922]
DNA gyrase subunit B in Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6059] [UniProtKB: A0QNE0]
ChEMBL	Inhibition of Mycobacterium smegmatis 155 6His-tagged DNA gyrase B catalytic domain ATPase activity expressed in Escherichia coli BL21 (DE3) pLysS cells assessed as inorganic phosphate release after 120 mins in presence of ATP by malachite green dye based assay	B	7.34	pIC50	46	nM	IC50	Eur J Med Chem (2016) 122: 216-231 [PMID:27371925]
DNA polymerase theta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6025] [GtoPdb: 3230] [UniProtKB: O75417]
ChEMBL	Inhibition of POLQ (unknown origin)	B	4.62	pIC50	24000	nM	IC50	J Med Chem (2023) 66: 6498-6522 [PMID:37134182]
DNA topoisomerase 4 subunit B in S.aureus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2150841] [UniProtKB: P0C1S7]
ChEMBL	Inhibition of Staphylococcus aures ParE using pBR322 DNA as substrate in presence of ATP	B	5.1	pIC50	7900	nM	IC50	Eur J Med Chem (2020) 188: 112022-112022 [PMID:31901744]
Genome polyprotein in Zika virus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523954] [UniProtKB: A0A024AXB9]
ChEMBL	Inhibition of Zika virus NS2B-NS3 protease	B	4.85	pIC50	14200	nM	IC50	Bioorg Med Chem Lett (2020) 30: 126965-126965 [PMID:31980339]
ChEMBL	Inhibition of Zika virus NS2B-NS3 protease by cell based assay	B	4.61	pEC50	24800	nM	EC50	Bioorg Med Chem Lett (2020) 30: 126965-126965 [PMID:31980339]
Microtubule-associated proteins 1A/1B light chain 3A in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4879518] [UniProtKB: Q9H492]
ChEMBL	Binding affinity to human LC3A expressed in Escherichia coli BL21-(DE3) by isothermal titration calorimetry	B	5.12	pKd	7500	nM	Kd	J Med Chem (2021) 64: 3720-3746 [PMID:33769048]
ChEMBL	Displacement of sGFP-p62-LIR peptide from human N-terminal SNAP-fused LC3A by time-resolved FRET assay	B	5.06	pKi	8800	nM	Ki	J Med Chem (2021) 64: 3720-3746 [PMID:33769048]
ChEMBL	Displacement of sGFP-p62-LIR peptide from human N-terminal SNAP-fused LC3A by time-resolved FRET assay	B	4.37	pIC50	42900	nM	IC50	J Med Chem (2021) 64: 3720-3746 [PMID:33769048]
Microtubule-associated proteins 1A/1B light chain 3B in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4879514] [UniProtKB: Q9GZQ8]
ChEMBL	Binding affinity to human LC3B expressed in Escherichia coli BL21-(DE3) by isothermal titration calorimetry	B	4.76	pKd	17400	nM	Kd	J Med Chem (2021) 64: 3720-3746 [PMID:33769048]
ChEMBL	Displacement of sGFP-p62-LIR peptide from human N-terminal SNAP-fused LC3B by time-resolved FRET assay	B	4.71	pKi	19300	nM	Ki	J Med Chem (2021) 64: 3720-3746 [PMID:33769048]
ParE in Streptococcus pneumoniae (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075022] [UniProtKB: Q7X1P7]
ChEMBL	Inhibition of Streptococcus pneumoniae SP-3 parE after 30 mins	B	5.69	pIC50	2030	nM	IC50	Bioorg Med Chem Lett (2009) 19: 5302-5306 [PMID:19683922]
Topoisomerase IV in S.aureus (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL3038508] [UniProtKB: P0C1S7, P0C1U9]
ChEMBL	Inhibition of Staphylococcus aureus DNA topoisomerase 4 decatenation activity	B	4.46	pIC50	35000	nM	IC50	Bioorg Med Chem (2016) 24: 6291-6297 [PMID:27143131]
ChEMBL	Inhibition of Staphylococcus aureus DNA topoisomerase 4 using supercoiled pNO1 plasmid DNA as substrate incubated for 30 mins by fluorescence assay	B	4.57	pIC50	27000	nM	IC50	Medchemcomm (2019) 10: 1007-1017 [PMID:31303999]
ChEMBL	Inhibition of Staphylococcus aureus DNA topoisomerase 4 using relaxed pNO1 plasmid and biotinylated oligonucelotide incubated for 30 mins by fluorescence based DNA relaxation assay	B	4.57	pIC50	27000	nM	IC50	J Med Chem (2015) 58: 5501-5521 [PMID:26098163]
ChEMBL	Inhibition of Staphylococcus aureus DNA topoisomerase 4 using supercoiled pNO1 plasmid DNA as substrate incubated for 30 mins by fluorescence assay	B	4.57	pIC50	27000	nM	IC50	ACS Med Chem Lett (2020) 11: 2433-2440 [PMID:33329764]
ChEMBL	Inhibition of Staphylococcus aureus DNA topoisomerase 4 using biotinylated oligonucleotide as substrate incubated for 30 mins in presence of ATP by fluorescence-based microplate reader assay	B	4.57	pIC50	27000	nM	IC50	Eur J Med Chem (2019) 167: 269-290 [PMID:30776691]
ChEMBL	Inhibition of Staphylococcus aureus DNA topoisomerase 4 using kinetoplast DNA as substrate by decatenation assay	B	4.7	pIC50	20000	nM	IC50	J Med Chem (2015) 58: 5501-5521 [PMID:26098163]
ChEMBL	Inhibition of Staphylococcus aureus topoisomerase 4 decatenation incubated for 30 mins in presence of ATP by ethidium bromide staining based analysis	B	4.88	pIC50	13265	nM	IC50	J Med Chem (2023) 66: 3968-3994 [PMID:36877255]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]