ChEMBL ligand: CHEMBL1622 (Acide folique, Acido folico, Acidum folicum, B03BB01, Bio science, Folacin, Folate, Foldine, Folettes, Foliamin, Folic acid, Folic Acid, Folicare, Folicet, Folipac, Folsan, Folvite, Incafolic, Lexpec, Millafol, Natur flow, NSC-3073, Preconceive, Pteroylglutamic acid, Roche, Vitamin b9, Vitamin bc, Vitamin Bc, Vitamin m, Vitamin M)
aldo-keto reductase family 1 member B/Aldose reductase in Human [ChEMBL: CHEMBL1900] [GtoPdb: 2768] [UniProtKB: P15121] There should be some charts here, you may need to enable JavaScript!
Dihydrofolate reductase in Escherichia coli [ChEMBL: CHEMBL1809] [UniProtKB: P0ABQ4] There should be some charts here, you may need to enable JavaScript!
Solute carrier organic anion transporter family member 1A3 in Rat [ChEMBL: CHEMBL2073707] [UniProtKB: P70502] There should be some charts here, you may need to enable JavaScript!
thymidylate synthetase/Thymidylate synthase in Human [ChEMBL: CHEMBL1952] [GtoPdb: 2642] [UniProtKB: P04818] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
aldo-keto reductase family 1 member B/Aldose reductase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1900] [GtoPdb: 2768] [UniProtKB: P15121]
ChEMBL	Inhibition of recombinant human ALR2 using D,L-glyceraldehyde and NADPH as substrate preincubated for 3 mins followed by substrate addition and measured for 3 mins by spectrophotometric analysis	B	4.28	pIC50	52500	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4870-4874 [PMID:27666634]
Dihydrofolate reductase in Escherichia coli (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1809] [UniProtKB: P0ABQ4]
ChEMBL	Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 7	B	5.32	pKd	4800	nM	Kd	J Med Chem (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Thermodynamic Dissociation Constant for compound-Tyr31-dihydrofolate reductase (DHFR) complex at pH 7	B	5.36	pKd	4400	nM	Kd	J Med Chem (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Thermodynamic Dissociation Constant for compound-Phe31-dihydrofolate reductase (DHFR) complex at pH 7	B	6.09	pKd	810	nM	Kd	J Med Chem (1988) 31: 129-137 [PMID:3275776]
Solute carrier organic anion transporter family member 1A3 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2073707] [UniProtKB: P70502]
ChEMBL	TP_TRANSPORTER: inhibition of MTX uptake in OAT-K1-expressing MDCK cells	F	4.85	pKi	14100	nM	Ki	J Pharmacol Exp Ther (2000) 293: 1034-1039 [PMID:10869408]
thymidylate synthetase/Thymidylate synthase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1952] [GtoPdb: 2642] [UniProtKB: P04818]
ChEMBL	Inhibitory constant of thymidylate synthase was determined in human AML cells	B	4.4	pKi	40000	nM	Ki	J Med Chem (1987) 30: 675-678 [PMID:3470522]

ChEMBL data shown on this page come from version 35:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]