ChEMBL ligand: CHEMBL1622 (Folacin, Vitamin Bc, Vitamin M, NSC-3073, Folicet, Natur flow, Preconceive, Roche, Bio science, Folsan, Folic Acid, Folic acid, Lexpec, Folicare, Folvite, B03BB01, Pteroylglutamic acid)
Dihydrofolate reductase in Escherichia coli [ChEMBL: CHEMBL5441] [UniProtKB: B0BL08] There should be some charts here, you may need to enable JavaScript!
Solute carrier organic anion transporter family member 1A3 in Rat [ChEMBL: CHEMBL2073707] [UniProtKB: P70502] There should be some charts here, you may need to enable JavaScript!
thymidylate synthetase/Thymidylate synthase in Human [ChEMBL: CHEMBL1952] [GtoPdb: 2642] [UniProtKB: P04818] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Dihydrofolate reductase in Escherichia coli (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5441] [UniProtKB: B0BL08]
ChEMBL	Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 7	B	5.32	pKd	4800	nM	Kd	J. Med. Chem. (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Thermodynamic Dissociation Constant for compound-Tyr31-dihydrofolate reductase (DHFR) complex at pH 7	B	5.36	pKd	4400	nM	Kd	J. Med. Chem. (1988) 31: 129-137 [PMID:3275776]
ChEMBL	Thermodynamic Dissociation Constant for compound-Phe31-dihydrofolate reductase (DHFR) complex at pH 7	B	6.09	pKd	810	nM	Kd	J. Med. Chem. (1988) 31: 129-137 [PMID:3275776]
Solute carrier organic anion transporter family member 1A3 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2073707] [UniProtKB: P70502]
ChEMBL	TP_TRANSPORTER: inhibition of MTX uptake in OAT-K1-expressing MDCK cells	F	4.85	pKi	14100	nM	Ki	J. Pharmacol. Exp. Ther. (2000) 293: 1034-1039 [PMID:10869408]
thymidylate synthetase/Thymidylate synthase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1952] [GtoPdb: 2642] [UniProtKB: P04818]
ChEMBL	Inhibitory constant of thymidylate synthase was determined in human AML cells	B	4.4	pKi	40000	nM	Ki	J. Med. Chem. (1987) 30: 675-678 [PMID:3470522]

ChEMBL data shown on this page come from version 28:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, Cibrián-Uhalte E, Davies M, Dedman N, Karlsson A, Magariños MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]