ouabain [Ligand Id: 4826] activity data from GtoPdb and ChEMBL
Click here for a description of the charts and data table
Please tell us if you are using this feature and what you think!
| ChEMBL ligand: CHEMBL222863 (Acocantherin, Gratus strophanthin, NSC-25485, Ouabain, Strophantin-g) |
|---|
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
| DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
|---|---|---|---|---|---|---|---|---|
| Kv11.1/HERG in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809] | ||||||||
| ChEMBL | Displacement of Tracer Red from human ERG expressed in cell membranes by fluorescence polarization assay | B | 5.24 | pIC50 | 5800 | nM | IC50 | J Med Chem (2018) 61: 1800-1820 [PMID:29291372] |
| sodium/potassium-transporting ATPase subunit α-1/sodium/potassium-transporting ATPase subunit β-1/Na+/K+ ATPase alpha-1/beta-1 in Rat (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4106147] [GtoPdb: 833, 837] [UniProtKB: P06685, P07340] | ||||||||
| ChEMBL | Inhibition of recombinant rat Na+/K+-ATPase alpha1/beta1 expressed in baculovirus infected insect Sf9 cell membranes using [gamma-32P]ATP as substrate preincubated for 20 mins followed by substrate addition measured after 30 mins by liquid scintillation counting | B | 5.37 | pIC50 | 4300 | nM | IC50 | J Med Chem (2018) 61: 1800-1820 [PMID:29291372] |
| sodium/potassium-transporting ATPase subunit α-2/sodium/potassium-transporting ATPase subunit β-1/Na+/K+ ATPase alpha-2/beta-1 in Rat (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4106148] [GtoPdb: 834, 837] [UniProtKB: P06686, P07340] | ||||||||
| ChEMBL | Inhibition of recombinant rat Na+/K+-ATPase alpha2/beta1 expressed in baculovirus infected insect Sf9 cell membranes using [gamma-32P]ATP as substrate preincubated for 20 mins followed by substrate addition measured after 30 mins by liquid scintillation counting | B | 6.77 | pIC50 | 170 | nM | IC50 | J Med Chem (2018) 61: 1800-1820 [PMID:29291372] |
| sodium/potassium-transporting ATPase subunit α-3/sodium/potassium-transporting ATPase subunit β-1/Na+/K+ ATPase alpha-3/beta-1 in Rat (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4106149] [GtoPdb: 835, 837] [UniProtKB: P06687, P07340] | ||||||||
| ChEMBL | Inhibition of recombinant rat Na+/K+-ATPase alpha3/beta1 expressed in baculovirus infected insect Sf9 cell membranes using [gamma-32P]ATP as substrate preincubated for 20 mins followed by substrate addition measured after 30 mins by liquid scintillation counting | B | 7.51 | pIC50 | 31 | nM | IC50 | J Med Chem (2018) 61: 1800-1820 [PMID:29291372] |
| sodium/potassium-transporting ATPase subunit β-1/sodium/potassium-transporting ATPase subunit α-4/N+/K+ ATPase alpha-4/beta-1 in Rat (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4106165] [GtoPdb: 837, 836] [UniProtKB: P07340, Q64541] | ||||||||
| ChEMBL | Inhibition of recombinant rat Na+/K+-ATPase alpha4/beta1 expressed in baculovirus infected insect Sf9 cell membranes using [gamma-32P]ATP as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins in presence of Na+, K+ and Mg2+ by liquid scintillation counting | B | 8.37 | pIC50 | 4.3 | nM | IC50 | J Med Chem (2018) 61: 1800-1820 [PMID:29291372] |
| sodium/potassium-transporting ATPase subunit β-3/sodium/potassium-transporting ATPase subunit α-4/N+/K+ ATPase alpha-4/beta-3 in Rat (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4106166] [GtoPdb: 839, 836] [UniProtKB: Q63377, Q64541] | ||||||||
| ChEMBL | Inhibition of recombinant rat Na+/K+-ATPase alpha4/beta3 expressed in baculovirus infected Sf9 cell membranes using [gamma-32P]ATP as substrate preincubated for 20 mins followed by substrate addition measured after 30 mins by liquid scintillation counting | B | 8.74 | pIC50 | 1.8 | nM | IC50 | J Med Chem (2018) 61: 1800-1820 [PMID:29291372] |
| Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364] | ||||||||
| ChEMBL | Antiplasmodial activity against Plasmodium falciparum 3D7 after 72 hrs by SYBR green assay | F | 4.9 | pIC50 | 12589.25 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
| ChEMBL | Antiplasmodial activity against Plasmodium falciparum 7G8 after 72 hrs by SYBR green assay | F | 4.9 | pIC50 | 12589.25 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
| sodium/potassium-transporting ATPase subunit α-1/sodium/potassium-transporting ATPase subunit β-1/sodium/potassium-transporting ATPase subunit α-3/sodium/potassium-transporting ATPase subunit β-2/sodium/potassium-transporting ATPase subunit α-2/sodium/potassium-transporting ATPase subunit β-3/sodium/potassium-transporting ATPase subunit γ/sodium/potassium-transporting ATPase subunit α-4/Sodium/potassium-transporting ATPase in Human (target type: PROTEIN COMPLEX GROUP) [ChEMBL: CHEMBL2095186] [GtoPdb: 833, 837, 835, 838, 834, 839, 2610, 836] [UniProtKB: P05023, P05026, P13637, P14415, P50993, P54709, P54710, Q13733] | ||||||||
| ChEMBL | Inhibition of KCl-stimulated Na+/K+-ATPase in human placental membrane vesicles measured for 60 secs by 3-O-methylfluorescein phosphate dye based fluorometric method | B | 5.18 | pIC50 | 6600 | nM | IC50 | J Nat Prod (2017) 80: 1575-1583 [PMID:28452477] |
| ChEMBL | Inhibition of human Na(+)/K(+) ATPase assessed as reduction in ouabain-sensitive and K(+)-dependent cleavage of fluorescent pseudosubstrate 3-O-methylfluorescein phosphate by fluorometric method | B | 5.22 | pIC50 | 6000 | nM | IC50 | J Nat Prod (2011) 74: 1805-1811 [PMID:21827183] |
ChEMBL data shown on this page come from version 34:
Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]
