7NI [Ligand Id: 5127] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL247378 (7-Nitroindazole)
  • Neuronal NOS/Nitric-oxide synthase, brain in Human [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475]
  • Neuronal NOS/Nitric-oxide synthase, brain in Rat [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476]
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  • Endothelial NOS/Nitric-oxide synthase, endothelial in Human [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474]
  • Nitric-oxide synthase, endothelial in Bovine [ChEMBL: CHEMBL4802] [UniProtKB: P29473]
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  • Inducible NOS/Nitric oxide synthase, inducible in Human [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228]
  • Inducible NOS/Nitric oxide synthase, inducible in Mouse [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477]
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DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
Neuronal NOS/Nitric-oxide synthase, brain in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475]
GtoPdb - - 5.3 pIC50 5000 nM IC50 Br. J. Pharmacol. (1993) 110: 225-8 [PMID:7693279]
ChEMBL Inhibitory activity against human neuronal nitric oxide synthase (nNOS) B 5.54 pIC50 2870 nM IC50 Bioorg. Med. Chem. Lett. (1999) 9: 2569-2572 [PMID:10498210]
ChEMBL In vitro inhibition of human neuronal nitric oxide synthase B 5.6 pIC50 2500 nM IC50 J. Med. Chem. (2004) 47: 1575-1586 [PMID:14998342]
ChEMBL Inhibition of NOS1 B 6.15 pIC50 710 nM IC50 Bioorg. Med. Chem. (2009) 17: 6180-6187 [PMID:19679481]
Neuronal NOS/Nitric-oxide synthase, brain in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476]
ChEMBL Inhibition of rat recombinant nNOS expressed in Saccharomyces cerevisiae assessed as conversion of [gamma-14C]L-arginine to [14C]L-citrulline by radioactive assay B 4.57 pIC50 27000 nM IC50 Eur. J. Med. Chem. (2009) 44: 2877-2887 [PMID:19144449]
ChEMBL Inhibition of rat brain recombinant nNOS B 4.6 pIC50 25000 nM IC50 Bioorg. Med. Chem. (2008) 16: 5962-5973 [PMID:18502134]
ChEMBL Inhibitory activity against rat neuronal nitric oxide synthase (nNOS) B 5.67 pIC50 2120 nM IC50 Bioorg. Med. Chem. Lett. (1999) 9: 2569-2572 [PMID:10498210]
ChEMBL Tested to inhibit the conversion of [3H]L-arginine to [3H]L-citrulline catalyzed by neuronal NOS from rat cerebellum. B 5.7 pIC50 2000 nM IC50 Bioorg. Med. Chem. Lett. (1995) 5: 1573-1576
ChEMBL In vitro inhibitory concentration against rat cerebellar neuronal nitric oxide synthase B 6.1 pIC50 800 nM IC50 Bioorg. Med. Chem. Lett. (2001) 11: 1153-1156 [PMID:11354365]
ChEMBL Inhibition of rat neuronal NOS B 6.22 pIC50 600 nM IC50 Bioorg. Med. Chem. Lett. (2007) 17: 3177-3180 [PMID:17395463]
ChEMBL Inhibition of nNOS in Sprague-Dawley rat brain homogenates assessed as conversion of oxyhemoglobin to methemoglobin measured for 10 mins by UV-visible spectrophotometer analysis B 6.96 pIC50 110 nM IC50 Bioorg. Med. Chem. (2011) 19: 3935-3944 [PMID:21665485]
Endothelial NOS/Nitric-oxide synthase, endothelial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474]
ChEMBL Tested to inhibit the conversion of [3H]-L-arginine to [3H]L-citrulline catalyzed by endothelial NOS from human umbilical vein endothelial cells. B 4.44 pIC50 36000 nM IC50 Bioorg. Med. Chem. Lett. (1995) 5: 1573-1576
ChEMBL In vitro inhibition of human endothelial nitric oxide synthase B 5.6 pIC50 2500 nM IC50 J. Med. Chem. (2004) 47: 1575-1586 [PMID:14998342]
ChEMBL Inhibitory activity against human endothelial nitric oxide synthase (eNOS) B 5.72 pIC50 1890 nM IC50 Bioorg. Med. Chem. Lett. (1999) 9: 2569-2572 [PMID:10498210]
Nitric-oxide synthase, endothelial in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4802] [UniProtKB: P29473]
ChEMBL Inhibition of bovine recombinant eNOS expressed in Escherichia coli assessed as conversion of [gamma-14C]L-arginine to [14C]L-citrulline by radioactive assay B 4.39 pIC50 41000 nM IC50 Eur. J. Med. Chem. (2009) 44: 2877-2887 [PMID:19144449]
ChEMBL Inhibition of bovine recombinant eNOS B 4.46 pIC50 35000 nM IC50 Bioorg. Med. Chem. (2008) 16: 5962-5973 [PMID:18502134]
Inducible NOS/Nitric oxide synthase, inducible in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228]
ChEMBL Tested to inhibit the conversion of [3H]L-arginine to [3H]L-citrulline catalyzed by inducible NOS from human DLD-1 cells. B 4.62 pIC50 24000 nM IC50 Bioorg. Med. Chem. Lett. (1995) 5: 1573-1576
ChEMBL Inhibition of NOS2 B 5.24 pIC50 5800 nM IC50 Bioorg. Med. Chem. (2009) 17: 6180-6187 [PMID:19679481]
Inducible NOS/Nitric oxide synthase, inducible in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477]
ChEMBL Inhibition of mouse recombinant iNOS B 4.3 pIC50 50000 nM IC50 Bioorg. Med. Chem. (2008) 16: 5962-5973 [PMID:18502134]
ChEMBL Inhibition of mouse recombinant iNOS expressed in Escherichia coli assessed as conversion of [gamma-14C]L-arginine to [14C]L-citrulline by radioactive assay B 4.4 pIC50 40000 nM IC50 Eur. J. Med. Chem. (2009) 44: 2877-2887 [PMID:19144449]
ChEMBL Tested to inhibit the conversion of [3H]L-arginine to [3H]L-citrulline catalyzed by inducible NOS from mouse macrophage J774 cells B 4.7 pIC50 20000 nM IC50 Bioorg. Med. Chem. Lett. (1995) 5: 1573-1576

ChEMBL data shown on this page come from version 26:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, CibriƔn-Uhalte E, Davies M, Dedman N, Karlsson A, MagariƱos MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]