SKI II [Ligand Id: 6041] activity data from GtoPdb and ChEMBL

Click here for a description of the charts and data table

Please tell us if you are using this feature and what you think!

ChEMBL ligand: CHEMBL1076555
  • 5-LOX/Arachidonate 5-lipoxygenase in Human [ChEMBL: CHEMBL215] [GtoPdb: 1385] [UniProtKB: P09917]
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
  • sphingosine kinase 1/Sphingosine kinase 1 in Human [ChEMBL: CHEMBL4394] [GtoPdb: 2204] [UniProtKB: Q9NYA1]
There should be some charts here, you may need to enable JavaScript!
  • sphingosine kinase 2/Sphingosine kinase 2 in Human [ChEMBL: CHEMBL3023] [GtoPdb: 2205] [UniProtKB: Q9NRA0]
  • sphingosine kinase 2/Sphingosine kinase 2 in Mouse [ChEMBL: CHEMBL1075305] [GtoPdb: 2205] [UniProtKB: Q9JIA7]
There should be some charts here, you may need to enable JavaScript!
  • Transitional endoplasmic reticulum ATPase in Human [ChEMBL: CHEMBL1075145] [UniProtKB: P55072]
There should be some charts here, you may need to enable JavaScript!
  • delta 4-desaturase, sphingolipid 1 in Human [GtoPdb: 2484] [UniProtKB: O15121]
There should be some charts here, you may need to enable JavaScript!
DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
5-LOX/Arachidonate 5-lipoxygenase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL215] [GtoPdb: 1385] [UniProtKB: P09917]
ChEMBL Inhibition of 5-lipoxygenase in human PMNL using arachidonic acid as substrate preincubated for 15 mins before substrate addition measured after 10 mins B 6.15 pIC50 700 nM IC50 Eur. J. Med. Chem. (2014) 84: 302-311 [PMID:25036790]
ChEMBL Inhibition of partially purified recombinant 5-lipoxygenase (unknown origin) using arachidonic acid as substrate preincubated for 15 mins before substrate addition measured after 10 mins by HPLC analysis B 7.52 pIC50 30 nM IC50 Eur. J. Med. Chem. (2014) 84: 302-311 [PMID:25036790]
Salivary alpha-amylase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2478] [UniProtKB: P0DUB6]
ChEMBL Inhibition of human salivary alpha-amylase using GalG2CNP as substrate by UV-Vis spectrophotometric analysis B 2.98 pIC50 1043000 nM IC50 Bioorg. Med. Chem. (2015) 23: 6725-6732 [PMID:26395057]
sphingosine kinase 1/Sphingosine kinase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4394] [GtoPdb: 2204] [UniProtKB: Q9NYA1]
ChEMBL Inhibition of human recombinant SphK1 expressed in Sf9 cells assessed as radiolabeled products using 5 uM sphingosine and 10 uM gamma[32P]ATP by liquid scintillation counting B 4.77 pKi 17000 nM Ki J. Med. Chem. (2015) 58: 1879-1899 [PMID:25643074]
ChEMBL Inhibition of recombinant human GST-tagged SphK1 using [3H]-sphingosine as substrate after 30 mins by scintillation counting method B 4.77 pKi 17000 nM Ki Eur J Med Chem (2017) 139: 461-481 [PMID:28822281]
ChEMBL Inhibition of SPHK1 (unknown origin) B 4.77 pKi 17000 nM Ki Bioorg. Med. Chem. (2016) 24: 3218-3230 [PMID:27255176]
ChEMBL Inhibition of full-length SK1 (unknown origin) (2 to 384 residues) expressed in Escherichia coli using Sph as substrate by resorufin based fluorescence assay B 4.8 pKi 16000 nM Ki J Med Chem (2019) 62: 3658-3676 [PMID:30889352]
ChEMBL Inhibition of SK1 (unknown origin) B 4.8 pKi 16000 nM Ki J. Med. Chem. (2016) 59: 965-984 [PMID:26780304]
ChEMBL Inhibition of recombinant human SPHK1 expressed in Escherichia coli using [3-3H]sphingosine as substrate after 30 mins by scintillation counting B 4.8 pKi 16000 nM Ki J Med Chem (2017) 60: 3933-3957 [PMID:28406646]
GtoPdb - - 4.8 pKi 16000 nM Ki J Pharmacol Exp Ther (2010) 333: 129-39 [PMID:20061445]
ChEMBL Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cells B 4.92 pKi 12000 nM Ki J. Med. Chem. (2011) 54: 3524-3548 [PMID:21495716]
ChEMBL Inhibition of SPHK1 B 4.92 pKi 12000 nM Ki J. Med. Chem. (2011) 54: 3524-3548 [PMID:21495716]
ChEMBL Inhibition of purified human SphK1 assessed as inhibition of formation of [33P]-S1P after 50 mins by scintillation counting B 6.3 pKi 500 nM Ki Bioorg. Med. Chem. Lett. (2013) 23: 4608-4616 [PMID:23845219]
ChEMBL Inhibition of recombinant human SphK1 using NBD-sphingosine as substrate after 2 hrs in presence of ATP by HPLC method B 4.46 pIC50 35000 nM IC50 Bioorg Med Chem (2017) 25: 3046-3052 [PMID:28408190]
ChEMBL Inhibition of SK1 (unknown origin) B 4.46 pIC50 35000 nM IC50 J Med Chem (2018) 61: 9811-9840 [PMID:29969256]
GtoPdb Measured using a GST-hSPHK1 fusion protein - 6.3 pIC50 500 nM IC50 Cancer Res (2003) 63: 5962-9 [PMID:14522923]
sphingosine kinase 2/Sphingosine kinase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3023] [GtoPdb: 2205] [UniProtKB: Q9NRA0]
ChEMBL Inhibition of human recombinant SphK2 expressed in Sf9 cells assessed as radiolabeled products using 10 uM sphingosine and 10 uM gamma[32P]ATP by liquid scintillation counting B 4.3 pKi 50000 nM Ki J. Med. Chem. (2015) 58: 1879-1899 [PMID:25643074]
ChEMBL Inhibition of SPHK2 (unknown origin) B 4.3 pKi 50000 nM Ki Bioorg. Med. Chem. (2016) 24: 3218-3230 [PMID:27255176]
GtoPdb - - 5.1 pKi 7900 nM Ki PLoS ONE (2012) 7: e44543 [PMID:22970244]
ChEMBL Inhibition of SK2 (unknown origin) B 5.1 pKi 7900 nM Ki J. Med. Chem. (2016) 59: 965-984 [PMID:26780304]
ChEMBL Inhibition of recombinant human SPHK2 expressed in Escherichia coli using [3-3H]sphingosine as substrate after 30 mins by scintillation counting B 5.1 pKi 7900 nM Ki J Med Chem (2017) 60: 3933-3957 [PMID:28406646]
ChEMBL Inhibition of SK2 (unknown origin) B 5.17 pKi 6700 nM Ki J Med Chem (2019) 62: 3658-3676 [PMID:30889352]
ChEMBL Inhibition of recombinant human SphK2 using NBD-sphingosine as substrate after 2 hrs in presence of ATP by HPLC method B 4.7 pIC50 20000 nM IC50 Bioorg Med Chem (2017) 25: 3046-3052 [PMID:28408190]
ChEMBL Inhibition of SK2 (unknown origin) B 4.7 pIC50 20000 nM IC50 J Med Chem (2018) 61: 9811-9840 [PMID:29969256]
sphingosine kinase 2/Sphingosine kinase 2 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075305] [GtoPdb: 2205] [UniProtKB: Q9JIA7]
ChEMBL Inhibition of mouse recombinant SPHK2 expressed in baculovirus-infected Sf9 cells B 4.48 pKi 33000 nM Ki J. Med. Chem. (2011) 54: 3524-3548 [PMID:21495716]
Transitional endoplasmic reticulum ATPase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075145] [UniProtKB: P55072]
ChEMBL Inhibition of VCP assessed as conversion of ATP to ADP by enzyme coupled glucokinase assay B 7.15 pIC50 70 nM IC50 Bioorg. Med. Chem. Lett. (2010) 20: 1677-1679 [PMID:20137940]
ChEMBL Inhibition of VCP in human HeLa cells assessed as stabilization of ubiquitin-tagged luciferase after 24 hrs by reporter gene assay B 5.92 pEC50 <1200 nM EC50 Bioorg. Med. Chem. Lett. (2010) 20: 1677-1679 [PMID:20137940]
delta 4-desaturase, sphingolipid 1 in Human [GtoPdb: 2484] [UniProtKB: O15121]
GtoPdb - - 6.52 pKi 300 nM Ki J Lipid Res (2014) 55: 1711-20 [PMID:24875537]

ChEMBL data shown on this page come from version 28:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, CibriƔn-Uhalte E, Davies M, Dedman N, Karlsson A, MagariƱos MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]