ChEMBL ligand: CHEMBL596380 (3,4-Methylenedioxyb-Nitrostyrene)
NLRP3/NACHT, LRR and PYD domains-containing protein 3 in Mouse [ChEMBL: CHEMBL3779755] [GtoPdb: 1770] [UniProtKB: Q8R4B8] There should be some charts here, you may need to enable JavaScript!
Transitional endoplasmic reticulum ATPase in Human [ChEMBL: CHEMBL1075145] [UniProtKB: P55072] There should be some charts here, you may need to enable JavaScript!
SRC proto-oncogene, non-receptor tyrosine kinase in Human [GtoPdb: 2206] [UniProtKB: P12931] There should be some charts here, you may need to enable JavaScript!
spleen associated tyrosine kinase in Human [GtoPdb: 2230] [UniProtKB: P43405] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
NLRP3/NACHT, LRR and PYD domains-containing protein 3 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3779755] [GtoPdb: 1770] [UniProtKB: Q8R4B8]
ChEMBL	Inhibition of NLRP3 inflammasome activation in LPS-stimulated mouse bone marrow-derived macrophages assessed as reduction in IL-1beta secretion preincubated for 30 mins followed by nigericin addition and measured after 30 mins by ELISA	B	5.64	pIC50	2310	nM	IC50	J Med Chem (2021) 64: 13633-13657 [PMID:34506712]
ChEMBL	Inhibition of NLRP3 inflammasome activation in LPS/nigericin-treated C57BL/6 mouse bone marrow-derived macrophages assessed as reduction in IL-1beta secretion preincubated for 3 hrs with LPS and for 30 mins with compound prior to nigericin addition and measured after 30 mins by ELISA	B	5.64	pIC50	2310	nM	IC50	Eur J Med Chem (2022) 236: 114357-114357 [PMID:35428012]
ChEMBL	Inhibition of NLRP3 in LPS stimulated mouse BMDM cells after 15 mins followed by addition of ATP by ELISA analysis	B	5.7	pIC50	2000	nM	IC50	J Med Chem (2016) 59: 1691-1710 [PMID:26422006]
ChEMBL	Inhibition of NLRP3 inflammasome activation in LPS-primed mouse bone marrow derived macrophages preincubated for 15 mins followed by addition of ATP and measured after 30 mins by ELISA method	B	5.7	pIC50	2000	nM	IC50	Eur J Med Chem (2020) 185: 111822-111822 [PMID:31699536]
Transitional endoplasmic reticulum ATPase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075145] [UniProtKB: P55072]
ChEMBL	Inhibition of His6-tagged p97 (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as reduction in ATPase activity measured after 60 mins by biomol green reagent based assay	B	5.77	pIC50	1700	nM	IC50	Eur J Med Chem (2021) 213: 113148-113148 [PMID:33476933]
ChEMBL	Inhibition of p97 in human HeLa cells assessed as reduction in proteasomal turnover of p97-dependent reporter substrate UbG76V-GFP incubated for 120 mins by luciferase reporter gene assay	B	5.8	pIC50	1600	nM	IC50	Eur J Med Chem (2021) 213: 113148-113148 [PMID:33476933]
SRC proto-oncogene, non-receptor tyrosine kinase in Human [GtoPdb: 2206] [UniProtKB: P12931]
GtoPdb	-	-	4.56	pIC50	27300	nM	IC50	Biochem Pharmacol (2007) 74: 601-11 [PMID:17601492]
spleen associated tyrosine kinase in Human [GtoPdb: 2230] [UniProtKB: P43405]
GtoPdb	-	-	5.55	pIC50	2800	nM	IC50	Biochem Pharmacol (2007) 74: 601-11 [PMID:17601492]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]