ChEMBL ligand: CHEMBL796 (Concerta, Cotempla xr-odt, Daytrana, Methylin, Methylphenidate, Methylphenidate extended release, Prc-063, Threo-dl-methylphenidate)
DAT/Dopamine transporter in Human [ChEMBL: CHEMBL238] [GtoPdb: 927] [UniProtKB: Q01959] DAT/Dopamine transporter in Rat [ChEMBL: CHEMBL338] [GtoPdb: 927] [UniProtKB: P23977] There should be some charts here, you may need to enable JavaScript!
NET/Norepinephrine transporter in Human [ChEMBL: CHEMBL222] [GtoPdb: 926] [UniProtKB: P23975] There should be some charts here, you may need to enable JavaScript!
SERT/Serotonin transporter in Human [ChEMBL: CHEMBL228] [GtoPdb: 928] [UniProtKB: P31645] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
DAT/Dopamine transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL238] [GtoPdb: 927] [UniProtKB: Q01959]
ChEMBL	Displacement of [125I]RTI-55 from human DAT expressing HEK293 cells	B	6.96	pKi	110	nM	Ki	J Med Chem (2007) 50: 219-232 [PMID:17228864]
ChEMBL	Displacement of [3H]WIN 35,428 from human DAT expressed in HEK293E cells after 1 hr by liquid scintillation counting	B	7.47	pKi	34	nM	Ki	ACS Med Chem Lett (2014) 5: 760-765 [PMID:25050161]
GtoPdb	-	-	7.1	pIC50	79	nM	IC50	J Med Chem (2007) 50: 219-32 [PMID:17228864]
ChEMBL	Inhibition of [3H]DA uptake at human DAT expressing HEK293 cells	B	7.1	pIC50	79	nM	IC50	J Med Chem (2007) 50: 219-232 [PMID:17228864]
ChEMBL	Inhibition of [3H]WIN-35428 Binding to the Dopamine Transporter in Rhesus (Macaca mulatta) orCynomolgus Monkey (Macaca fascicularis) Caudate-Putamen	B	7.77	pIC50	17	nM	IC50	J Med Chem (2003) 46: 1538-1545 [PMID:12672255]
DAT/Dopamine transporter in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL338] [GtoPdb: 927] [UniProtKB: P23977]
ChEMBL	Tested for the ability to bind stereoselectively and enantioselectively to the dopamine transporter (DAT); Value ranges from 34 - 83 nM	B	7.08	pIC50	83	nM	IC50	J Med Chem (2003) 46: 1538-1545 [PMID:12672255]
NET/Norepinephrine transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL222] [GtoPdb: 926] [UniProtKB: P23975]
ChEMBL	Displacement of [125I]RTI-55 from human NET expressing HEK293 cells	B	6.18	pKi	660	nM	Ki	J Med Chem (2007) 50: 219-232 [PMID:17228864]
ChEMBL	Displacement of [3H]Nisoxetine from human NET expressed in HEK293E cells after 1 hr by liquid scintillation counting	B	6.47	pKi	340	nM	Ki	ACS Med Chem Lett (2014) 5: 760-765 [PMID:25050161]
GtoPdb	-	-	7.21	pIC50	61	nM	IC50
ChEMBL	Inhibition of [3H]NE uptake into human NET expressing HEK293 cells	B	7.21	pIC50	61	nM	IC50	J Med Chem (2007) 50: 219-232 [PMID:17228864]
SERT/Serotonin transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL228] [GtoPdb: 928] [UniProtKB: P31645]
ChEMBL	Displacement of [125I]RTI-55 from human SERT expressing HEK293 cells	B	4.19	pKi	65000	nM	Ki	J Med Chem (2007) 50: 219-232 [PMID:17228864]
ChEMBL	Displacement of [3H]Citolapram from human SERT expressed in HEK293E cells after 1 hr by liquid scintillation counting	B	5	pKi	>10000	nM	Ki	ACS Med Chem Lett (2014) 5: 760-765 [PMID:25050161]
ChEMBL	Inhibition of [3H]5-HT uptake into human SERT expressing HEK293 cells	B	5.29	pIC50	5100	nM	IC50	J Med Chem (2007) 50: 219-232 [PMID:17228864]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]