ChEMBL ligand: CHEMBL558642 (GSK-650394)
calcium/calmodulin dependent protein kinase kinase 2/Calcium/calmodulin-dependent protein kinase kinase 2 in Mouse [ChEMBL: CHEMBL4295888] [GtoPdb: 1957] [UniProtKB: Q8C078] There should be some charts here, you may need to enable JavaScript!
calcium/calmodulin dependent protein kinase kinase 1/CaM-kinase kinase alpha in Human [ChEMBL: CHEMBL5256] [GtoPdb: 1956] [UniProtKB: Q8N5S9] There should be some charts here, you may need to enable JavaScript!
calcium/calmodulin dependent protein kinase kinase 2/CaM-kinase kinase beta in Human [ChEMBL: CHEMBL5284] [GtoPdb: 1957] [UniProtKB: Q96RR4] There should be some charts here, you may need to enable JavaScript!
aurora kinase A/Serine/threonine-protein kinase Aurora-A in Human [ChEMBL: CHEMBL4722] [GtoPdb: 1936] [UniProtKB: O14965] There should be some charts here, you may need to enable JavaScript!
serum/glucocorticoid regulated kinase 1/Serine/threonine-protein kinase Sgk1 in Human [ChEMBL: CHEMBL2343] [GtoPdb: 1534] [UniProtKB: O00141] There should be some charts here, you may need to enable JavaScript!
serum/glucocorticoid regulated kinase 2 in Human [GtoPdb: 1535] [UniProtKB: Q9HBY8] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
calcium/calmodulin dependent protein kinase kinase 2/Calcium/calmodulin-dependent protein kinase kinase 2 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4295888] [GtoPdb: 1957] [UniProtKB: Q8C078]
ChEMBL	Inhibition of CAMKK2 in mouse N39 cells assessed as decrease in AMPKalpha phosphorylation at T172 preincubated for 80 mins followed by inomycin addition by ELISA	B	5.9	pIC50	1258.93	nM	IC50	Bioorg Med Chem Lett (2018) 28: 1958-1963 [PMID:29653895]
calcium/calmodulin dependent protein kinase kinase 1/CaM-kinase kinase alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5256] [GtoPdb: 1956] [UniProtKB: Q8N5S9]
ChEMBL	Inhibition of human CAMKK1 using CAMKKtide as substrate assessed as residual activity by [gamma-33P]-ATP assay relative to control	B	7.48	pIC50	33	nM	IC50	J Med Chem (2021) 64: 10849-10877 [PMID:34264658]
calcium/calmodulin dependent protein kinase kinase 2/CaM-kinase kinase beta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5284] [GtoPdb: 1957] [UniProtKB: Q96RR4]
ChEMBL	Inhibition of full-length human GST-tagged CAMKK2 using 5FAM-AKPKGNKDYHLQTCCGSLAYRRR-amide as substrate preincubated for 30 mins followed by substrate addition measured after 120 mins by fluorescence polarization assay	B	8.1	pIC50	7.94	nM	IC50	Bioorg Med Chem Lett (2018) 28: 1958-1963 [PMID:29653895]
ChEMBL	Inhibition of human CAMKK2 using CAMKKtide as substrate assessed as residual activity by [gamma-33P]-ATP assay relative to control	B	8.52	pIC50	3	nM	IC50	J Med Chem (2021) 64: 10849-10877 [PMID:34264658]
ChEMBL	Inhibition of NanoLuc-fused CAMKK2 (unknown origin) expressed in HEK293 cells after 24 hrs by NanoBRET assay	B	8.52	pIC50	<3	nM	IC50	J Med Chem (2021) 64: 10849-10877 [PMID:34264658]
aurora kinase A/Serine/threonine-protein kinase Aurora-A in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4722] [GtoPdb: 1936] [UniProtKB: O14965]
ChEMBL	Further kinetic evaluation of compounds supplied by Sanofi against AuroraA within a BIAcore3000 instrument	B	6.53	pKd	298.01	nM	Kd	K4DD drug target binding kinetics data
serum/glucocorticoid regulated kinase 1/Serine/threonine-protein kinase Sgk1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2343] [GtoPdb: 1534] [UniProtKB: O00141]
ChEMBL	Inhibition of SGK1-mediated epithelial sodium channel activity in human M-1 cells assessed as short circuit current by whole cell electrophysiological transepithelial experiment	B	6.24	pIC50	580	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4441-4445 [PMID:19497745]
GtoPdb	-	-	7.21	pIC50	62	nM	IC50	Cancer Res (2008) 68: 7475-83 [PMID:18794135]
ChEMBL	Inhibition of SGK1 by fluorescence polarization assay	B	7.89	pIC50	13	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4441-4445 [PMID:19497745]
ChEMBL	Inhibition of SGK1 expressed in Escherichia coli or baculovirus-infected insect cells by IMAP assay	B	7.89	pIC50	13	nM	IC50	J Med Chem (2011) 54: 5131-5143 [PMID:21699136]
serum/glucocorticoid regulated kinase 2 in Human [GtoPdb: 1535] [UniProtKB: Q9HBY8]
GtoPdb	-	-	6.99	pIC50	103	nM	IC50	Cancer Res (2008) 68: 7475-83 [PMID:18794135]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]