ChEMBL ligand: CHEMBL2152922 (Pf-04995274)
5-HT4 receptor/Serotonin 4 (5-HT4) receptor in Human [ChEMBL: CHEMBL1875] [GtoPdb: 9] [UniProtKB: Q13639] 5-HT4 receptor/Serotonin 4 (5-HT4) receptor in Rat [ChEMBL: CHEMBL4317] [GtoPdb: 9] [UniProtKB: Q62758] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
5-HT4 receptor/Serotonin 4 (5-HT4) receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1875] [GtoPdb: 9] [UniProtKB: Q13639]
ChEMBL	Displacement of [3H]GR113808 from human 5HT4B receptor expressed in HEK293 cells after 30 mins by liquid scintillation counting	B	9.34	pKi	0.46	nM	Ki	J Med Chem (2012) 55: 9240-9254 [PMID:22974325]
ChEMBL	Displacement of [3H]GR113808 from human 5HT4A receptor expressed in HEK293 cells after 30 mins by liquid scintillation counting	B	9.44	pKi	0.36	nM	Ki	J Med Chem (2012) 55: 9240-9254 [PMID:22974325]
ChEMBL	Displacement of [3H]GR113808 from human 5HT4E receptor expressed in CHO cells after 30 mins by liquid scintillation counting	B	9.49	pKi	0.32	nM	Ki	J Med Chem (2012) 55: 9240-9254 [PMID:22974325]
GtoPdb	Measured at the 5-HT4d receptor.	-	9.82	pKi	0.15	nM	Ki	J Med Chem (2012) 55: 9240-54 [PMID:22974325]
ChEMBL	Displacement of [3H]GR113808 from human 5HT4D receptor expressed in HEK293 cells after 30 mins by liquid scintillation counting	B	9.82	pKi	0.15	nM	Ki	J Med Chem (2012) 55: 9240-9254 [PMID:22974325]
ChEMBL	Agonist activity at recombinant human 5-HT4E receptor expressed in CHO cells assessed as induction of c-AMP accumulation after 4 hrs by luciferase reporter assay	B	8.82	pEC50	1.5	nM	EC50	J Med Chem (2018) 61: 4993-5008 [PMID:29763304]
ChEMBL	Agonist activity at human 5HT4e receptor expressed in CHO cells assessed as cAMP level after 4 hrs by luciferase reporter gene assay	F	8.89	pEC50	1.3	nM	EC50	Eur J Med Chem (2015) 103: 289-301 [PMID:26363507]
ChEMBL	Agonist activity at human 5HT4A receptor expressed in HEK293 cells assessed as increase in cAMP production after 30 mins by HTRF assay	F	9.33	pEC50	0.47	nM	EC50	J Med Chem (2012) 55: 9240-9254 [PMID:22974325]
ChEMBL	Agonist activity at human 5HT4B receptor expressed in HEK293 cells assessed as increase in cAMP production after 30 mins by HTRF assay	F	9.44	pEC50	0.36	nM	EC50	J Med Chem (2012) 55: 9240-9254 [PMID:22974325]
ChEMBL	Agonist activity at 5-HT4E receptor (unknown origin)	B	9.59	pEC50	0.26	nM	EC50	J Med Chem (2018) 61: 4993-5008 [PMID:29763304]
ChEMBL	Agonist activity at human 5HT4E receptor expressed in HEK293 cells assessed as increase in cAMP production after 30 mins by HTRF assay	F	9.59	pEC50	0.26	nM	EC50	J Med Chem (2012) 55: 9240-9254 [PMID:22974325]
ChEMBL	Partial agonist activity at human 5HT4D receptor expressed in HEK293 cells assessed as cAMP production after 30 mins by HTRF assay	F	9.7	pEC50	0.2	nM	EC50	J Med Chem (2012) 55: 9240-9254 [PMID:22974325]
5-HT4 receptor/Serotonin 4 (5-HT4) receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4317] [GtoPdb: 9] [UniProtKB: Q62758]
ChEMBL	Displacement of [3H]GR113808 from 5HT4 receptor in rat striatal membrane after 30 mins by liquid scintillation counting	B	9.52	pKi	0.3	nM	Ki	J Med Chem (2012) 55: 9240-9254 [PMID:22974325]
ChEMBL	Agonist activity at rat 5HT4L receptor expressed in HEK293 cells assessed as increase in cAMP production after 30 mins by HTRF assay	F	9.19	pEC50	0.65	nM	EC50	J Med Chem (2012) 55: 9240-9254 [PMID:22974325]
ChEMBL	Agonist activity at rat 5HT4E receptor expressed in HEK293 cells assessed as increase in cAMP production after 30 mins by HTRF assay	F	9.21	pEC50	0.62	nM	EC50	J Med Chem (2012) 55: 9240-9254 [PMID:22974325]
ChEMBL	Agonist activity at rat 5HT4S receptor expressed in HEK293 cells assessed as increase in cAMP production after 30 mins by HTRF assay	F	9.23	pEC50	0.59	nM	EC50	J Med Chem (2012) 55: 9240-9254 [PMID:22974325]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]