Gene and Protein Information
Species	TM	AA	Chromosomal Location	Gene Symbol	Gene Name	Reference
Plasmodium falciparum 3D7	-	876		CPSF3	cleavage and polyadenylation specificity factor subunit 3, putative

Previous and Unofficial Names

CPSF3 | Pf3D7_14_v3:1,557,114..1,561,108(-) | CPSF73 | CPSF-73 | PF14_0364

Database Links
Alphafold	Q8IL83 (Pf3D7)
PlasmoDB	PF3D7_1438500 (Pf3D7)
UniProtKB	Q8IL83 (Pf3D7)

Whole Organism Assay Data Linked to This Target
Key to terms and symbols		Click column headers to sort
Ligand Sp. Assay description Type Action Value Parameter Reference AN3661 PfK1 Plasmodium falciparum K1 P. falciparum strain K1 (PfK1) was isolated in Kanchanaburi, Thailand in 1979. PfK1 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be a multidrug-resistant strain. ✖ Parasite growth inhibition assay - - 7.7 pIC50 2 ⤷ pIC50 7.7 (IC50 2x10-8 M) [3H]-hypoxanthine incorporation [2] Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont) AN3661 PfFCR3 Plasmodium falciparum FCR3 P. falciparum strain FCR3 (PfFCR3) was originally isolated from a patient in The Gambia, West Africa in 1976. PfFCR3 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4). Differences in sensitivity of PfFCR3 clones to chloroquine have been reported, with the original isolate that was chloroquine sensitive rapidly acquiring resistance in culture (PMID: 7031656, PMID: 51801). ✖ Parasite growth inhibition assay - - 7.5 pIC50 2 ⤷ pIC50 7.5 (IC50 3x10-8 M) [3H]-hypoxanthine incorporation [2] Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont) AN3661 PfDd2 Plasmodium falciparum Dd2 P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy. PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine. ✖ Parasite growth inhibition assay - - 7.4 pIC50 2 ⤷ pIC50 7.4 (IC50 3.9x10-8 M) [3H]-hypoxanthine incorporation [2] Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont) AN3661 Pf3D7 Plasmodium falciparum 3D7 P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1). Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine. ✖ Parasite growth inhibition assay - - 7.3 pIC50 2 ⤷ pIC50 7.3 (IC50 5.2x10-8 M) [3H]-hypoxanthine incorporation [2] Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont) AN3661 PfHB3 Plasmodium falciparum HB3 P. falciparum strain HB3 (PfHB3) was cloned from the Honduras I/CDC strain, originally isolated from a patient in Choluteca, Honduras, during an outbreak of urban malaria in January 1980. PfHB3 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be chloroquine sensitive. ✖ Parasite growth inhibition assay - - 7.3 pIC50 2 ⤷ pIC50 7.3 (IC50 5.6x10-8 M) [3H]-hypoxanthine incorporation [2] Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)

Malaria Pharmacology Comments

Cleavage and Polyadenylation Specificity Factor subunit 3 (CPSF3 or CPSF73) is a 73kDa subunit of the CPSF protein complex. This complex directs the 3' cleavage and polyadenylation reaction that is an essential step in eukaryotic mRNA processing. CPSF3 is a zinc-dependent endonuclease that cleaves the mRNA precursor downstream of the polyadenylation signal sequence [1]. P. falciparum CPSF3 (PfCPSF3) has been identified as a possible target for the benzoxaborole hit compound AN3661 and may have potential as a target for antimalarial drug development [2].