K<SUB>v</SUB>12.3 | Voltage-gated potassium channels | IUPHAR/BPS Guide to PHARMACOLOGY

Kv12.3

Target id: 577

Nomenclature: Kv12.3

Family: Voltage-gated potassium channels

Annotation status:  image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates.  » Email us

   GtoImmuPdb view: OFF :     Currently no data for Kv12.3 in GtoImmuPdb

Gene and Protein Information
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 6 1 1017 17q21.2 KCNH4 potassium voltage-gated channel subfamily H member 4
Mouse 6 1 1018 11 D Kcnh4 potassium voltage-gated channel, subfamily H (eag-related), member 4
Rat 6 1 1017 10q32.1 Kcnh4 potassium voltage-gated channel subfamily H member 4
Previous and Unofficial Names
BEC2 | Elk1 | brain-specific eag-like channel 2 | ELK channel 1 | ether-a-go-go-like potassium channel 1 | potassium voltage-gated channel, subfamily H (eag-related), member 4 | potassium channel, voltage gated eag related subfamily H, member 4 | potassium voltage-gated channel
Database Links
CATH/Gene3D
Ensembl Gene
Entrez Gene
Human Protein Atlas
KEGG Gene
OMIM
RefSeq Nucleotide
RefSeq Protein
UniProtKB
Wikipedia
Ion Selectivity and Conductance
Species:  Rat
Macroscopic current rectification:  Ik
References:  1
Voltage Dependence
  V0.5 (mV)  τ (msec)  Reference  Cell type  Species 
Activation  -59.0 38.0 1 CHO cells. Rat
Inactivation  - -
Deactivation  - 200.0 1
Comments  τ deactivation at -80mV [1]
Gating Inhibitor Comments
Voltage clamp of Kv12.3 heterologously expressed in HEK293 cells demonstrated that external H+ is a gating inhibitor with a pKa of 7.1 and holding voltage -60mV [2].
Channel Blocker Comments
At millimolar concentrations, Ba2+ acts as a pore blocker for Kv12.3 [1].
Kv12.3 is resistant to block by external TEA, 4-AP, E-4031 [1].
Tissue Distribution
Cerebral cortex, occipital pole, fromtal lobe, tenporal lobe, putamen, caudate nucleus, hippocampus.
Species:  Human
Technique:  Northern blot
References:  3
Piriform cortex, olfactory tubercle, cerebral cortex layers II-VI, hippocampus, basal ganglia
Species:  Rat
Technique:  In situ hybridisation
References:  4
Pituitary and clonal somato-mammotroph cells (GH3 / B6 cells)
Species:  Rat
Technique:  RT-PCR
References:  5
Lung, spinal cord, hippocampus, cortex, striatum, pituitary, medulla
Species:  Rat
Technique:  RT-PCR
References:  1
Brain, testes
Species:  Rat
Technique:  Northern blot
References:  1
Functional Assays
Membrane currents recorded from heterologously expressed channels using whole-cell patch clamp
Species:  Rat
Tissue:  CHO (Chinese Hamster Ovary) cells
Response measured:  Remarkably low threshold of activation, may contribute to the maintenance of the resting potential. Activation kinetics not dependent on prepulse potential.
References:  1
General Comments
This channel has:
  • GFG (rather than the common GYG) potassium channel signature sequence
  • a PAS domain in the distal part of the cytosolic N-terminus
  • a cNBD domain in the proximal portion of the C-terminus

References

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1. Engeland B, Neu A, Ludwig J, Roeper J, Pongs O. (1998) Cloning and functional expression of rat ether-à-go-go-like K+ channel genes. J. Physiol. (Lond.), 513 ( Pt 3): 647-54. [PMID:9824707]

2. Kazmierczak M, Zhang X, Chen B, Mulkey DK, Shi Y, Wagner PG, Pivaroff-Ward K, Sassic JK, Bayliss DA, Jegla T. (2013) External pH modulates EAG superfamily K+ channels through EAG-specific acidic residues in the voltage sensor. J. Gen. Physiol., 141 (6): 721-35. [PMID:23712551]

3. Miyake A, Mochizuki S, Yokoi H, Kohda M, Furuichi K. (1999) New ether-à-go-go K(+) channel family members localized in human telencephalon. J. Biol. Chem., 274 (35): 25018-25. [PMID:10455180]

4. Saganich MJ, Machado E, Rudy B. (2001) Differential expression of genes encoding subthreshold-operating voltage-gated K+ channels in brain. J. Neurosci., 21 (13): 4609-24. [PMID:11425889]

5. Wulfsen I, Hauber HP, Schiemann D, Bauer CK, Schwarz JR. (2000) Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary. J. Neuroendocrinol., 12 (3): 263-72. [PMID:10718922]

Contributors

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How to cite this page

Michael C. Sanguinetti, K. George Chandy, Stephan Grissmer, George A. Gutman, Michel Lazdunski, David Mckinnon, Luis A. Pardo, Gail A. Robertson, Bernardo Rudy, Walter Stühmer, Xiaoliang Wang.
Voltage-gated potassium channels: Kv12.3. Last modified on 07/10/2014. Accessed on 15/11/2018. IUPHAR/BPS Guide to PHARMACOLOGY, http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=577.