Compound class:
Synthetic organic
Comment: Fluorescent MIF ligand B is reported in Cisneros et al. (2016) [1]. It is a macrophage migration inhibitory factor (MIF) inhibitor tethered to fluorescein to generate a probe suitable for fluorescence polarization assay determination of dissociation constant (Kd) of MIF inhibitors as an alternative to the Ki or IC50 values that are obtained from tautomerization assays.
|
|
Immunopharmacology Comments |
Fluorescent MIF ligand B is a tracer that can be used to determine MIF binding affinity [1], and is a more reliable alternative to the commonly used, but inconsistent [2], MIF enzymatic activity assays that have been used previously. It will also facilitate the identification of MIF inhibitors that are devoid of tautomerase inhibitory action. |