Compound class:
Synthetic organic
Comment: Fluorescent MIF ligand B is reported in Cisneros et al. (2016) [1]. It is a macrophage migration inhibitory factor (MIF) inhibitor tethered to fluorescein to generate a probe suitable for fluorescence polarization assay determination of dissociation constant (Kd) of MIF inhibitors as an alternative to the Ki or IC50 values that are obtained from tautomerization assays.
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References |
1. Cisneros JA, Robertson MJ, Valhondo M, Jorgensen WL. (2016)
A Fluorescence Polarization Assay for Binding to Macrophage Migration Inhibitory Factor and Crystal Structures for Complexes of Two Potent Inhibitors. J Am Chem Soc, 138 (27): 8630-8. [PMID:27299179] |
2. Cisneros JA, Robertson MJ, Valhondo M, Jorgensen WL. (2016)
Irregularities in enzyme assays: The case of macrophage migration inhibitory factor. Bioorg Med Chem Lett, 26 (12): 2764-2767. [PMID:27156768] |