ChEMBL ligand: CHEMBL3764617
sphingosine kinase 1/Sphingosine kinase 1 in Human [ChEMBL: CHEMBL4394] [GtoPdb: 2204] [UniProtKB: Q9NYA1] There should be some charts here, you may need to enable JavaScript!
sphingosine kinase 2/Sphingosine kinase 2 in Human [ChEMBL: CHEMBL3023] [GtoPdb: 2205] [UniProtKB: Q9NRA0] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
sphingosine kinase 1/Sphingosine kinase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4394] [GtoPdb: 2204] [UniProtKB: Q9NYA1]
ChEMBL	Inhibition of human SK1 using D-erythro sphingosine as substrate and gamma[33P]ATP by Lineweaver-Burk plot analysis	B	4.57	pKi	27000	nM	Ki	J Med Chem (2016) 59: 965-984 [PMID:26780304]
ChEMBL	Inhibition of recombinant human C-terminal TEV cleavage site-fused-His6-tagged SphK1 expressed in baculovirus infected sf9 insect cells using d-erythro-sphingosine as substrate in presence of [gamma32P]-ATP and ATP by radioactivity-based assay	B	4.57	pKi	27000	nM	Ki	J Med Chem (2020) 63: 1178-1198 [PMID:31895563]
ChEMBL	Inhibition of recombinant human SphK1 expressed in baculovirus infected Sf9 cells assessed as decrease in [33P]SIP production using D-erythro-sphingosine as substrate in presence of [gamma33P]-ATP by scintillation counting method	B	4.57	pKi	27000	nM	Ki	Bioorg Med Chem (2021) 30: 115941-115941 [PMID:33385956]
GtoPdb	In vitro inhibition of substrate phosphorylation.	-	4.57	pIC50	27000	nM	IC50	Oncotarget (2015) 6: 7065-83 [PMID:25788259]
sphingosine kinase 2/Sphingosine kinase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3023] [GtoPdb: 2205] [UniProtKB: Q9NRA0]
GtoPdb	In vitro inhibition of substrate phosphorylation.	-	5.16	pKi	6900	nM	Ki	Oncotarget (2015) 6: 7065-83 [PMID:25788259]
ChEMBL	Inhibition of human SK2 using D-erythro sphingosine as substrate and gamma[33P]ATP by Lineweaver-Burk plot analysis	B	5.16	pKi	6900	nM	Ki	J Med Chem (2016) 59: 965-984 [PMID:26780304]
ChEMBL	Inhibition of recombinant human His6/HA-fusion-tagged SphK2 expressed in baculovirus infected sf9 insect cells using d-erythro-sphingosine as substrate in presence of [gamma32P]-ATP and ATP by radioactivity-based assay	B	5.16	pKi	6900	nM	Ki	J Med Chem (2020) 63: 1178-1198 [PMID:31895563]
ChEMBL	Inhibition of recombinant human SphK2 assessed as decrease in [33P]SIP production using sphingosine as substrate in presence of [gamma33P]-ATP by scintillation counting method	B	5.16	pKi	6900	nM	Ki	Bioorg Med Chem (2021) 30: 115941-115941 [PMID:33385956]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]