ChEMBL ligand: CHEMBL2374220 (GS-5885, Ledipasvir, Ledipasvir acetonate)
Nonstructural protein 5A in Hepatitis C virus [ChEMBL: CHEMBL3307224] [UniProtKB: Q5L478] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Nonstructural protein 5A in Hepatitis C virus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3307224] [UniProtKB: Q5L478]
ChEMBL	Inhibition of NS5A in HCV genotype 3a by cell based luciferase reporter gene assay	B	7.39	pIC50	41	nM	IC50	J Med Chem (2019) 62: 10563-10582 [PMID:31710479]
ChEMBL	Inhibition of NS5A in HCV genotype 2a JFH-1 by cell based luciferase reporter gene assay	B	7.68	pIC50	21	nM	IC50	J Med Chem (2019) 62: 10563-10582 [PMID:31710479]
ChEMBL	Inhibition of NS5A R44K mutant in HCV genotype 2b infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	6.03	pEC50	943	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in wild type HCV genotype 2b MD2b-1 infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	6.06	pEC50	865	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in HCV genotype 2b MD2b-1 infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	6.28	pEC50	530	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A P58S mutant in HCV genotype 2b infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	6.53	pEC50	292	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in HCV genotype 6e D88 infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	6.58	pEC50	264	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in HCV genotype 2a J6 infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	6.6	pEC50	249	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A N62V mutant in HCV genotype 2a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	6.65	pEC50	222	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in wild type HCV genotype 2b AY232738 infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	6.68	pEC50	211	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in HCV wild type genotype 2a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	6.68	pEC50	209	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A Q30E mutant in HCV genotype 1a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	6.77	pEC50	169	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in HCV genotype 3a S52 infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	6.77	pEC50	168	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A K44R mutant in HCV genotype 2a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	6.79	pEC50	164	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A N62S mutant in HCV genotype 2a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	6.91	pEC50	123	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A A30K mutant in HCV genotype 3a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	7.05	pEC50	>88.8	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A Y93H mutant in HCV genotype 3a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	7.05	pEC50	>88.8	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A Y93H mutant in HCV genotype 1a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	7.28	pEC50	52	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A Y93C mutant in HCV genotype 1a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	7.3	pEC50	49.6	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in wild type HCV genotype 3a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	7.35	pEC50	>44.8	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in HCV genotype 2a JFH1 infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	7.68	pEC50	21	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A Q30R mutant in HCV genotype 1a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	7.71	pEC50	19.6	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A L31M mutant in HCV genotype 1a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	7.77	pEC50	17	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A Y93H mutant in HCV genotype 1b infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	8.14	pEC50	7.2	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A Q30H mutant in HCV genotype 1a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	8.24	pEC50	5.7	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A M28T mutant in HCV genotype 1a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	8.72	pEC50	1.9	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in HCV genotype 6a HK6 infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	8.96	pEC50	1.1	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in HCV genotype 4a ED43 infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	9.41	pEC50	0.39	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in HCV genotype 5a SA13 infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	9.82	pEC50	0.15	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in wild type HCV genotype 1a infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	10.51	pEC50	0.03	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]
ChEMBL	Inhibition of NS5A in HCV genotype 1a H77 infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay	B	10.51	pEC50	0.03	nM	EC50	Bioorg Med Chem Lett (2019) 29: 2415-2427 [PMID:31230974]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]