ChEMBL ligand: CHEMBL185951 (JNJ-10191584)
H3 receptor/Histamine H3 receptor in Human [ChEMBL: CHEMBL264] [GtoPdb: 264] [UniProtKB: Q9Y5N1] There should be some charts here, you may need to enable JavaScript!
H4 receptor/Histamine H4 receptor in Human [ChEMBL: CHEMBL3759] [GtoPdb: 265] [UniProtKB: Q9H3N8] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
H3 receptor/Histamine H3 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL264] [GtoPdb: 264] [UniProtKB: Q9Y5N1]
ChEMBL	Binding affinity to human histamine H3 receptor	B	4.7	pKi	20000	nM	Ki	Bioorg Med Chem Lett (2012) 22: 1156-1159 [PMID:22189138]
ChEMBL	Binding affinity to histamine H3 receptor	B	4.85	pKi	14053	nM	Ki	J Med Chem (2005) 48: 8289-8298 [PMID:16366610]
ChEMBL	Binding affinity towards human histamine H3 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	B	5.4	pKi	3981.07	nM	Ki	Bioorg Med Chem Lett (2004) 14: 5251-5256 [PMID:15454206]
ChEMBL	Effective concentration required against human histamine H3 receptor was determined by the inhibition of the cAMP stimulated beta-galactosidase transcription in SK-N-MC cells	B	5.5	pEC50	3162.28	nM	EC50	Bioorg Med Chem Lett (2004) 14: 5251-5256 [PMID:15454206]
H4 receptor/Histamine H4 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3759] [GtoPdb: 265] [UniProtKB: Q9H3N8]
ChEMBL	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	F	7.7	pKd	19.95	nM	Kd	J Med Chem (2005) 48: 8289-8298 [PMID:16366610]
GtoPdb	-	-	7.1	pKi	-	-	-	Bioorg Med Chem Lett (2004) 14: 5251-6 [PMID:15454206]
ChEMBL	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	B	7.1	pKi	79.43	nM	Ki	Bioorg Med Chem Lett (2004) 14: 5251-5256 [PMID:15454206]
ChEMBL	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	B	7.32	pKi	47.7	nM	Ki	Bioorg Med Chem Lett (2011) 21: 6591-6595 [PMID:21955944]
ChEMBL	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	B	7.32	pKi	47.7	nM	Ki	Bioorg Med Chem Lett (2012) 22: 1156-1159 [PMID:22189138]
ChEMBL	Antagonist activity at human histamine H4 receptor by functional assay	F	7.4	pKi	39.6	nM	Ki	Bioorg Med Chem Lett (2011) 21: 6591-6595 [PMID:21955944]
ChEMBL	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	F	7.4	pKi	39.6	nM	Ki	Bioorg Med Chem Lett (2012) 22: 1156-1159 [PMID:22189138]
ChEMBL	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	B	7.59	pKi	26	nM	Ki	Bioorg Med Chem Lett (2012) 22: 461-467 [PMID:22153663]
ChEMBL	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	B	7.59	pKi	26	nM	Ki	J Med Chem (2005) 48: 8289-8298 [PMID:16366610]
ChEMBL	Displacement of [3H]histamine from human histamine H4 receptor	B	7.59	pKi	26	nM	Ki	Bioorg Med Chem Lett (2011) 21: 3113-3116 [PMID:21458260]
ChEMBL	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	B	7.7	pKi	20	nM	Ki	J Med Chem (2014) 57: 2429-2439 [PMID:24495018]
ChEMBL	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	F	6.28	pIC50	530	nM	IC50	J Med Chem (2005) 48: 8289-8298 [PMID:16366610]
ChEMBL	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	F	6.86	pIC50	138	nM	IC50	J Med Chem (2005) 48: 8289-8298 [PMID:16366610]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]