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ChEMBL ligand: CHEMBL4633662 |
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DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
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BLM RecQ like helicase/Bloom syndrome protein in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1293237] [GtoPdb: 3260] [UniProtKB: P54132] | ||||||||
ChEMBL | Binding affinity to His-tagged BLM I1168A mutant (unknown origin) expressed in Escherichia coli BL21 (DE3) cells by isothermal calorimetric titration assay | B | 5.18 | pKd | 6540 | nM | Kd | J Med Chem (2020) 63: 9752-9772 [PMID:32697083] |
ChEMBL | Binding affinity to His-tagged BLM H996A mutant (unknown origin) expressed in Escherichia coli BL21 (DE3) cells by isothermal calorimetric titration assay | B | 5.85 | pKd | 1400 | nM | Kd | J Med Chem (2020) 63: 9752-9772 [PMID:32697083] |
ChEMBL | Binding affinity to wild-type His-tagged BLM (642 to 1296 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells by isothermal calorimetric titration assay | B | 6.12 | pKd | 760 | nM | Kd | J Med Chem (2020) 63: 9752-9772 [PMID:32697083] |
GtoPdb | Binding affinity for wild-type BLM (642-1296) expressed in Escherichia coli, setermined by isothermal calorimetric titration (ICT) assay. | - | 6.12 | pKd | 760 | nM | Kd | J Med Chem (2020) 63: 9752-9772 [PMID:32697083] |
ChEMBL | Binding affinity to His-tagged BLM (642 to 1296 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells assessed as inhibition of protein interaction with 5'-biotin-labeled forked DNA measured after 1 hr by ELISA | B | 5.06 | pIC50 | 8700 | nM | IC50 | J Med Chem (2020) 63: 9752-9772 [PMID:32697083] |
ChEMBL | Inhibition of His-tagged BLM (642 to 1296 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells assessed as reduction in DNA unwinding activity using 5'-biotin-labeled forked DNA as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr by electrophoretic mobility shift assay | B | 6.1 | pIC50 | 800 | nM | IC50 | J Med Chem (2020) 63: 9752-9772 [PMID:32697083] |
ChEMBL data shown on this page come from version 33:
Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]