oxytocin [Ligand Id: 2174] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL395429 (Oxytocin, TTA-121, Pitocin, Syntometrine, Orasthin, Syntocinon)
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  • V1A receptor/Vasopressin V1a receptor in Human [ChEMBL: CHEMBL1889] [GtoPdb: 366] [UniProtKB: P37288]
  • V1A receptor in Rat [GtoPdb: 366] [UniProtKB: P30560]
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  • V1B receptor/Vasopressin V1b receptor in Human [ChEMBL: CHEMBL1921] [GtoPdb: 367] [UniProtKB: P47901]
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  • V2 receptor/Vasopressin V2 receptor in Human [ChEMBL: CHEMBL1790] [GtoPdb: 368] [UniProtKB: P30518]
  • V2 receptor/Vasopressin V2 receptor in Rat [ChEMBL: CHEMBL3766] [GtoPdb: 368] [UniProtKB: Q00788]
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DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
OT receptor/Oxytocin receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2049] [GtoPdb: 369] [UniProtKB: P30559]
ChEMBL Compound was evaluated for its dissociation constant (Kd) to guinea pig myometrial Oxytocin receptor B 8.17 pKd 6.7 nM Kd Bioorg. Med. Chem. Lett. (1999) 9: 667-672 [PMID:10201826]
ChEMBL Inhibition of radioligand [3H]OT binding to oxytocin receptor (OT) in guinea pig myometrium membrane B 8.17 pKd 6.7 nM Kd J. Med. Chem. (1994) 37: 255-259 [PMID:7507528]
ChEMBL Competitive binding to SNAP-tagged oxytocin receptor (unknown origin) expressed in HEK293 cells incubated for 1 hr at RT followed by 4 hrs at 4 degC by TR-FRET assay in presence of 5-(3-(22-(3-carboxy-4-(3-oxo-3H-xanthen-9-yl)phenyl)-15,22-dioxo-2,5,8,11-tetraoxa-14,21-diazadocosyl)-5-(3-(2-chloro-4-fluorophenoxy)azetidin-1-yl)-4H-1,2,4-triazol-4-yl)-2-methoxypyridinium 2,2,2-trifluoroacetate B 8.02 pKi 9.53 nM Ki J. Med. Chem. (2015) 58: 2547-2552 [PMID:25642985]
ChEMBL Binding affinity to oxytocin receptor B 8.17 pKi 6.8 nM Ki Bioorg. Med. Chem. Lett. (2009) 19: 990-994 [PMID:19095447]
ChEMBL Competitive binding to SNAP-tagged oxytocin receptor (unknown origin) expressed in HEK293 cells incubated for 1 hr at RT followed by 4 hrs at 4 degC by TR-FRET assay in presence of 2-(5-(3-(1-(5-(3-(2-chloro-4-fluorophenoxy)azetidin-1-yl)-4-(6-methoxypyridinium-3-yl)-4H-1,2,4-triazol-3-yl)-15-oxo-2,5,8,11-tetraoxa-14-azaoctadecan-18-yl)-1-ethyl-3-methyl-5-sulfoindolin-2-ylidene)penta-1,3-dienyl)-1-ethyl-3,3-dimethyl-3H-indolium-6-sulfonate 2,2,2-trifluoroacetate B 8.18 pKi 6.6 nM Ki J. Med. Chem. (2015) 58: 2547-2552 [PMID:25642985]
ChEMBL Displacement of [3H]OT from human mammary gland oxytocin receptor expressed in HEK293 cell membrane after 1 hr by scintillation counting method B 8.92 pKi 1.2 nM Ki Bioorg Med Chem (2018) 26: 3039-3045 [PMID:29602673]
ChEMBL Displacement of [Tyrosyl-2,6-3H]-Oxytocin from recombinant human oxytocin receptor expressed in CHO-DUKX-A2 cells after 180 mins by liquid scintillation counting analysis B 9.1 pKi 0.8 nM Ki Eur. J. Med. Chem. (2016) 108: 730-740 [PMID:26741855]
ChEMBL Binding affinity for human oxytocin receptor B 9.1 pKi 0.8 nM Ki J. Med. Chem. (2002) 45: 2579-2588 [PMID:12036367]
ChEMBL Binding affinity to OTR (unknown origin) B 9.1 pKi 0.79 nM Ki J. Med. Chem. (2015) 58: 2547-2552 [PMID:25642985]
ChEMBL Displacement of [3H]OT from oxytocin receptor expressed in COS1 cells B 9.1 pKi 0.79 nM Ki J. Med. Chem. (2010) 53: 8585-8596 [PMID:21117646]
ChEMBL Competitive binding to oxytocin receptor (unknown origin) by radioligand binding assay B 9.1 pKi 0.79 nM Ki J. Med. Chem. (2015) 58: 2547-2552 [PMID:25642985]
ChEMBL Displacement of [3H]8-arginine-vasopressin from human oxytocin receptor expressed in CHO cell membrane incubated for 1 hr by liquid scintillation counting method B 9.36 pKi 0.44 nM Ki Bioorg. Med. Chem. (2016) 24: 3513-3520 [PMID:27297999]
GtoPdb - - 9.6 pKi - - - Br J Obstet Gynaecol (1999) 106: 1047-53 [PMID:10519430];
EMBO J. (1995) 14: 2176-82 [PMID:7774575];
J. Pharmacol. Exp. Ther. (2003) 306: 253-61 [PMID:12660315];
Br J Pharmacol (2005) 146: 744-751 [PMID:16158071];
Life Sci. (1995) 57: 2253-61 [PMID:7475979];
FEBS Lett. (1996) 397: 201-6 [PMID:8955347]
ChEMBL Displacement of [3H]OT from human OT receptor expressed in HEK cells B 8.57 pIC50 2.7 nM IC50 Eur. J. Med. Chem. (2007) 42: 799-806 [PMID:17316912]
ChEMBL Agonist activity at human OTR receptor expressed in HEK293FT cells incubated for 30 mins by beta-arrestin recruitment assay F 7.59 pEC50 26 nM EC50 J Med Chem (2018) 61: 8670-8692 [PMID:30199637]
ChEMBL Activity at oxytocin receptor expressed in COS1 cells assessed as IP-one generation by HTRF assay B 7.82 pEC50 15 nM EC50 J. Med. Chem. (2010) 53: 8585-8596 [PMID:21117646]
ChEMBL Agonist activity at human oxytocin receptor expressed CHO cells assessed as induction of phospholipase C activity after 15 mins by inositol phosphate accumulation assay F 7.97 pEC50 10.8 nM EC50 J. Med. Chem. (2011) 54: 2864-2877 [PMID:21428295]
ChEMBL Agonist activity at C-terminal GFP-tagged OTR (unknown origin) expressed in HEK293 cells measured after 18 hrs by luciferase reporter gene assay B 8.05 pEC50 9 nM EC50 Bioorg Med Chem Lett (2017) 27: 2331-2335 [PMID:28438540]
ChEMBL Agonist activity at SNAP-tagged oxytocin receptor (unknown origin) expressed in HEK293 cells assessed as intracellular calcium flux after 3 mins by fluorescence assay F 8.1 pEC50 8 nM EC50 J. Med. Chem. (2015) 58: 2547-2552 [PMID:25642985]
ChEMBL Agonist activity at wild type OTR (unknown origin) expressed in HEK293 cells assessed as intracellular calcium flux after 3 mins by fluorescence assay F 8.3 pEC50 5 nM EC50 J. Med. Chem. (2015) 58: 2547-2552 [PMID:25642985]
ChEMBL Agonist activity at recombinant human oxytocin receptor expressed in CHO-DUKX-A2 cells assessed as change in intracellular calcium level by FLIPR assay B 8.52 pEC50 3 nM EC50 Eur. J. Med. Chem. (2016) 108: 730-740 [PMID:26741855]
ChEMBL Agonist activity at human oxytocin receptor expressed in CHO-K1 cells after 5 hrs by firefly luciferase reporter gene assay B 8.64 pEC50 2.3 nM EC50 J. Med. Chem. (2014) 57: 5306-5317 [PMID:24874785]
ChEMBL Agonist activity at human OTR receptor expressed in HEK293FT cells assessed as stimulation of calcium release by Aequorin based assay F 9.15 pEC50 0.7 nM EC50 J Med Chem (2018) 61: 8670-8692 [PMID:30199637]
ChEMBL Agonist activity at human mammary gland oxytocin receptor expressed in CHO cells assessed as increase in intracellular calcium flux measured for 5 mins by Fluo-4-AM dye based FLIPR assay F 9.52 pEC50 0.3 nM EC50 Bioorg Med Chem (2018) 26: 3039-3045 [PMID:29602673]
ChEMBL Agonist activity at human oxytocin receptor expressed in CHO cells assessed as increase in intracellular calcium flux measured for 90 sec by fluo-4 dye based FLIPR assay B 10.4 pEC50 0.04 nM EC50 Bioorg. Med. Chem. (2016) 24: 3513-3520 [PMID:27297999]
ChEMBL Agonist activity at human OTR expressed in CHO cells assessed as increase in calcium flux after 60 to 120 mins by fluo-4 dye based FLIPR assay F 10.4 pEC50 0.04 nM EC50 Bioorg Med Chem Lett (2018) 28: 415-419
OT receptor/Oxytocin receptor in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3243907] [GtoPdb: 369] [UniProtKB: P97926]
ChEMBL Agonist activity at mouse OTR receptor expressed in HEK293FT cells assessed as stimulation of calcium release by Aequorin based assay F 8.7 pEC50 2 nM EC50 J Med Chem (2018) 61: 8670-8692 [PMID:30199637]
OT receptor/Oxytocin receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3996] [GtoPdb: 369] [UniProtKB: P70536]
GtoPdb - - 8.7 pKi - - - J. Pharmacol. Exp. Ther. (2003) 306: 253-61 [PMID:12660315]
ChEMBL Binding affinity against Oxytocin receptor was determined in rat uterine membrane using radioligand [3H]oxytocin B 8.77 pKi 1.7 nM Ki J. Med. Chem. (1997) 40: 864-876 [PMID:9083475]
ChEMBL Binding affinity against oxytocin receptor in rat uterus B 9.05 pKi 0.89 nM Ki J. Med. Chem. (1990) 33: 2321-2323 [PMID:2167976]
V1A receptor/Vasopressin V1a receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1889] [GtoPdb: 366] [UniProtKB: P37288]
ChEMBL Binding affinity for human Vasopressin V1a receptor B 6.92 pKi 120 nM Ki J. Med. Chem. (2002) 45: 2579-2588 [PMID:12036367]
ChEMBL Binding affinity to V1aR (unknown origin) B 6.92 pKi 120 nM Ki J. Med. Chem. (2015) 58: 2547-2552 [PMID:25642985]
ChEMBL Binding affinity to vasopressin V1a receptor B 7.46 pKi 34.9 nM Ki Bioorg. Med. Chem. Lett. (2009) 19: 990-994 [PMID:19095447]
ChEMBL Displacement of [3H]AVP from human liver vasopressin V1a receptor expressed in HEK293 cell membrane after 1 hr by scintillation counting method B 7.7 pKi 20 nM Ki Bioorg Med Chem (2018) 26: 3039-3045 [PMID:29602673]
GtoPdb - - 8.3 pKi - - - J. Biol. Chem. (1994) 269: 3304-10 [PMID:8106369];
J. Biol. Chem. (1997) 272: 26536-44 [PMID:9334232];
Br. J. Pharmacol. (1998) 125: 1463-70 [PMID:9884074];
Endocrinology (1997) 138: 4109-22 [PMID:9322919];
Naunyn Schmiedebergs Arch. Pharmacol. (1998) 357: 63-9 [PMID:9459574];
Biochem Pharmacol (1997) 53: 1711-1717 [PMID:9264324];
J. Pharmacol. Exp. Ther. (2003) 306: 253-61 [PMID:12660315];
Br J Obstet Gynaecol (1999) 106: 1047-53 [PMID:10519430]
ChEMBL Antagonist activity at human vasopressin V1a expressed in AVP-stimulated HEK293 cells after 5 hrs by firefly luciferase reporter gene assay B 5 pIC50 >10000 nM IC50 J. Med. Chem. (2014) 57: 5306-5317 [PMID:24874785]
ChEMBL Agonist activity at human V1a receptor expressed in HEK293FT cells assessed as stimulation of calcium release by Aequorin based assay F 6.59 pEC50 260 nM EC50 J Med Chem (2018) 61: 8670-8692 [PMID:30199637]
ChEMBL Agonist activity at human vasopressin V1a expressed in HEK293 cells after 5 hrs by firefly luciferase reporter gene assay B 8 pEC50 10 nM EC50 J. Med. Chem. (2014) 57: 5306-5317 [PMID:24874785]
ChEMBL Agonist activity at human liver vasopressin V1a receptor expressed in CHO cells assessed as increase in intracellular calcium flux measured for 5 mins by Fluo-4-AM dye based FLIPR assay F 8.05 pEC50 9 nM EC50 Bioorg Med Chem (2018) 26: 3039-3045 [PMID:29602673]
ChEMBL Agonist activity at vasopressin 1a receptor (unknown origin) F 8.11 pEC50 7.7 nM EC50 Bioorg Med Chem Lett (2018) 28: 415-419
V1A receptor in Rat [GtoPdb: 366] [UniProtKB: P30560]
GtoPdb - - 6.1 pKi - - - EMBO J. (1995) 14: 2176-82 [PMID:7774575]
V1B receptor/Vasopressin V1b receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1921] [GtoPdb: 367] [UniProtKB: P47901]
ChEMBL Displacement of [3H]AVP from human vasopressin V1b receptor expressed in HEK293 cell membrane after 1 hr by scintillation counting method B 5.22 pKi >6000 nM Ki Bioorg Med Chem (2018) 26: 3039-3045 [PMID:29602673]
ChEMBL Binding affinity to V1bR (unknown origin) B 5.75 pKi 1782 nM Ki J. Med. Chem. (2015) 58: 2547-2552 [PMID:25642985]
GtoPdb - - 7 pKi - - - Br. J. Pharmacol. (1998) 125: 1463-70 [PMID:9884074];
Endocrinology (1997) 138: 4109-22 [PMID:9322919];
Naunyn Schmiedebergs Arch. Pharmacol. (1998) 357: 63-9 [PMID:9459574];
Biochem Pharmacol (1997) 53: 1711-1717 [PMID:9264324];
Br J Pharmacol (2005) 146: 744-751 [PMID:16158071]
ChEMBL Agonist activity at human vasopressin V1b expressed in HEK293 cells after 5 hrs by firefly luciferase reporter gene assay B 6.62 pEC50 240 nM EC50 J. Med. Chem. (2014) 57: 5306-5317 [PMID:24874785]
ChEMBL Agonist activity at human V1b receptor expressed in HEK293FT cells assessed as stimulation of calcium release by Aequorin based assay F 7.41 pEC50 39 nM EC50 J Med Chem (2018) 61: 8670-8692 [PMID:30199637]
ChEMBL Agonist activity at human vasopressin V1b receptor expressed in CHO cells assessed as increase in intracellular calcium flux measured for 5 mins by Fluo-4-AM dye based FLIPR assay F 7.48 pEC50 33 nM EC50 Bioorg Med Chem (2018) 26: 3039-3045 [PMID:29602673]
ChEMBL Agonist activity at human V1b receptor expressed in HEK293FT cells incubated for 30 mins by beta-arrestin recruitment assay F 7.54 pEC50 29 nM EC50 J Med Chem (2018) 61: 8670-8692 [PMID:30199637]
ChEMBL Agonist activity at human vasopressin V1b receptor expressed CHO cells assessed as induction of phospholipase C activity after 15 mins by inositol phosphate accumulation assay F 7.61 pEC50 24.5 nM EC50 J. Med. Chem. (2011) 54: 2864-2877 [PMID:21428295]
V2 receptor/Vasopressin V2 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1790] [GtoPdb: 368] [UniProtKB: P30518]
ChEMBL Displacement of [3H]AVP from human kidney vasopressin V2 receptor expressed in HEK293 cell membrane after 1 hr by scintillation counting method B 5.22 pKi >6000 nM Ki Bioorg Med Chem (2018) 26: 3039-3045 [PMID:29602673]
ChEMBL Binding affinity towards human Vasopressin V2 receptor B 5.46 pKi 3500 nM Ki J. Med. Chem. (2002) 45: 2579-2588 [PMID:12036367]
ChEMBL Binding affinity to V2R (unknown origin) B 5.81 pKi 1544 nM Ki J. Med. Chem. (2015) 58: 2547-2552 [PMID:25642985]
GtoPdb - - 6.8 pKi - - - Br. J. Pharmacol. (1998) 125: 1463-70 [PMID:9884074];
Endocrinology (1997) 138: 4109-22 [PMID:9322919];
Naunyn Schmiedebergs Arch. Pharmacol. (1998) 357: 63-9 [PMID:9459574];
Biochem Pharmacol (1997) 53: 1711-1717 [PMID:9264324];
EMBO J. (1995) 14: 2176-82 [PMID:7774575];
J. Pharmacol. Exp. Ther. (2003) 306: 253-61 [PMID:12660315]
ChEMBL Agonist activity at human V2 receptor expressed in HEK293FT cells incubated for 30 mins by beta-arrestin recruitment assay F 6.09 pEC50 810 nM EC50 J Med Chem (2018) 61: 8670-8692 [PMID:30199637]
ChEMBL Agonist activity at human V2 receptor expressed in HEK293FT cells assessed as stimulation of calcium release by Aequorin based assay F 6.68 pEC50 210 nM EC50 J Med Chem (2018) 61: 8670-8692 [PMID:30199637]
ChEMBL Agonist activity at human kidney vasopressin V2 receptor expressed in CHO cells assessed as increase in intracellular calcium flux measured for 5 mins by Fluo-4-AM dye based FLIPR assay F 7.54 pEC50 29 nM EC50 Bioorg Med Chem (2018) 26: 3039-3045 [PMID:29602673]
ChEMBL Agonist activity at human vasopressin V2 expressed in HEK293 cells after 5 hrs by firefly luciferase reporter gene assay B 8.14 pEC50 7.3 nM EC50 J. Med. Chem. (2014) 57: 5306-5317 [PMID:24874785]
V2 receptor/Vasopressin V2 receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3766] [GtoPdb: 368] [UniProtKB: Q00788]
ChEMBL Binding affinity against Vasopressin receptor in rat kidney medulla B 7.06 pKi 88 nM Ki J. Med. Chem. (1990) 33: 2321-2323 [PMID:2167976]
ChEMBL Binding affinity against Vasopressin receptor in rat liver B 7.21 pKi 61 nM Ki J. Med. Chem. (1990) 33: 2321-2323 [PMID:2167976]

ChEMBL data shown on this page come from version 27:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, CibriƔn-Uhalte E, Davies M, Dedman N, Karlsson A, MagariƱos MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]