ChEMBL ligand: CHEMBL482477
Macrophage migration inhibitory factor in Human [ChEMBL: CHEMBL2085] [UniProtKB: P14174] Macrophage migration inhibitory factor in Rat [ChEMBL: CHEMBL4524037] [UniProtKB: P30904] There should be some charts here, you may need to enable JavaScript!
Peroxisome proliferator-activated receptor-γ/Peroxisome proliferator-activated receptor gamma in Human [ChEMBL: CHEMBL235] [GtoPdb: 595] [UniProtKB: P37231] There should be some charts here, you may need to enable JavaScript!
Transient receptor potential cation channel subfamily A member 1 in Rhesus macaque [ChEMBL: CHEMBL6066835] [UniProtKB: A0A1D5QMT1] TRPA1/Transient receptor potential cation channel subfamily A member 1 in Human [ChEMBL: CHEMBL6007] [GtoPdb: 485] [UniProtKB: O75762] TRPA1/Transient receptor potential cation channel subfamily A member 1 in Mouse [ChEMBL: CHEMBL1075310] [GtoPdb: 485] [UniProtKB: Q8BLA8] TRPA1/Transient receptor potential cation channel subfamily A member 1 in Rat [ChEMBL: CHEMBL5160] [GtoPdb: 485] [UniProtKB: Q6RI86] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Macrophage migration inhibitory factor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2085] [UniProtKB: P14174]
ChEMBL	Inhibition of [3H]-BTZO-1 binding to recombinant human C-terminal His-tagged MIF expressed in Escherichia coli BL21 (DE3) measured after 3 hrs by SPA	B	5.44	pIC50	3600	nM	IC50	Chem Biol (2010) 17: 1282-1294 [PMID:21168764]
Macrophage migration inhibitory factor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4524037] [UniProtKB: P30904]
ChEMBL	Inhibition of recombinant rat MIF expressed in Escherichia coli BL21 (DE3) assessed as reduction in tautomerase activity using L-dopachrome carboxy-methyl ester as substrate by spectrophotometry	B	5.43	pIC50	3700	nM	IC50	Chem Biol (2010) 17: 1282-1294 [PMID:21168764]
Peroxisome proliferator-activated receptor-γ/Peroxisome proliferator-activated receptor gamma in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL235] [GtoPdb: 595] [UniProtKB: P37231]
ChEMBL	Binding affinity to PPARgamma	B	5.7	pKd	2000	nM	Kd	J Med Chem (2012) 55: 4027-4061 [PMID:22260081]
GtoPdb	-	-	6.3	pIC50	-	-	-	Proc Natl Acad Sci USA (1997) 94: 4318-23 [PMID:9113987]; Cell (1995) 83: 813-9 [PMID:8521498]; Anal Biochem (1998) 257: 112-9 [PMID:9514791]; Cell Signal (2002) 14: 573-83 [PMID:11955950]; Biochem Biophys Res Commun (2005) 337: 521-5 [PMID:16198309]
Transient receptor potential cation channel subfamily A member 1 in Rhesus macaque (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6066835] [UniProtKB: A0A1D5QMT1]
ChEMBL	Agonist activity at rhesus monkey TRPA1 transfected in HEK293F cells assessed as increase in Ca2+ influx by FLIPR calcium assay	B	4	pEC50	>100000	nM	EC50	J Pharmacol Exp Ther (2012) 341: 360-368 [PMID:22319196]
TRPA1/Transient receptor potential cation channel subfamily A member 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6007] [GtoPdb: 485] [UniProtKB: O75762]
ChEMBL	Agonist activity at full length human TRPA1 transfected in HEK293F cells assessed as increase in Ca2+ influx by FLIPR calcium assay	B	4.39	pEC50	40400	nM	EC50	J Pharmacol Exp Ther (2012) 341: 360-368 [PMID:22319196]
ChEMBL	Activation of TRPA1 channel	B	5.25	pEC50	5600	nM	EC50	J Med Chem (2010) 53: 5085-5107 [PMID:20356305]
TRPA1/Transient receptor potential cation channel subfamily A member 1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075310] [GtoPdb: 485] [UniProtKB: Q8BLA8]
ChEMBL	Agonist activity at full length mouse TRPA1 transfected in HEK293F cells assessed as increase in Ca2+ influx by FLIPR calcium assay	B	4.25	pEC50	56400	nM	EC50	J Pharmacol Exp Ther (2012) 341: 360-368 [PMID:22319196]
TRPA1/Transient receptor potential cation channel subfamily A member 1 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5160] [GtoPdb: 485] [UniProtKB: Q6RI86]
ChEMBL	Agonist activity at full length rat TRPA1 transfected in HEK293F cells assessed as increase in Ca2+ influx by FLIPR calcium assay	B	4.7	pEC50	19900	nM	EC50	J Pharmacol Exp Ther (2012) 341: 360-368 [PMID:22319196]

ChEMBL data shown on this page come from version 36:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]