22R-hydroxycholesterol [Ligand Id: 2742] activity data from GtoPdb and ChEMBL

Click here for a description of the charts and data table

Please tell us if you are using this feature and what you think!

ChEMBL ligand: CHEMBL422904 (22(R)-Hydroxycholesterol)
  • Liver X receptor-α/LXR-alpha in Human [ChEMBL: CHEMBL2808] [GtoPdb: 602] [UniProtKB: Q13133]
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
  • RAR-related orphan receptor-γ/Nuclear receptor ROR-gamma in Human [ChEMBL: CHEMBL1741186] [GtoPdb: 600] [UniProtKB: P51449]
There should be some charts here, you may need to enable JavaScript!
  • Farnesoid X receptor in Human [GtoPdb: 603] [UniProtKB: Q96RI1]
There should be some charts here, you may need to enable JavaScript!
DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
Liver X receptor-α/LXR-alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2808] [GtoPdb: 602] [UniProtKB: Q13133]
ChEMBL Effective concentration for cofactor association with recombinant liver X receptor-alpha B 4.82 pEC50 15000 nM EC50 Bioorg Med Chem Lett (2005) 15: 2824-2828 [PMID:15911262]
ChEMBL Agonist activity at human recombinant LXRalpha expressed in Escherichia coli BL21 cells assessed as association of recombinant SRC1 to LXRalpha ligand binding domain by HTRF assay B 4.82 pEC50 >15000 nM EC50 J Nat Prod (2005) 68: 1247-1252 [PMID:16124770]
ChEMBL Activation of GAL4 fused human LXR alpha expressed in HEK293 cells after 24 hrs by luciferase reporter gene assay B 5.17 pEC50 6710 nM EC50 J Med Chem (2017) 60: 6548-6562 [PMID:28741954]
GtoPdb - - 5.3 pEC50 - - - Nature (1996) 383: 728-31 [PMID:8878485]
ChEMBL Agonist activity at human LXRalpha-LBD assessed as recruitment of co-activator peptide after 2 hrs by TR-FRET assay B 6 pEC50 1000 nM EC50 Bioorg Med Chem Lett (2013) 23: 4185-4190 [PMID:23769638]
ChEMBL Concentration required for half maximal activity was calculated in human nuclear oxysterol receptor liver X receptor-alpha in LiSA. B 6.49 pEC50 325 nM EC50 J Med Chem (2001) 44: 886-897 [PMID:11300870]
Liver X receptor-β/LXR-beta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4093] [GtoPdb: 601] [UniProtKB: P55055]
ChEMBL Effective concentration for cofactor association with recombinant liver X receptor-beta B 4.82 pEC50 15000 nM EC50 Bioorg Med Chem Lett (2005) 15: 2824-2828 [PMID:15911262]
ChEMBL Agonist activity at human recombinant LXRbeta expressed in Escherichia coli BL21 cells assessed as association of recombinant SRC1 to LXRbeta ligand binding domain by HTRF assay B 4.82 pEC50 >15000 nM EC50 J Nat Prod (2005) 68: 1247-1252 [PMID:16124770]
ChEMBL Activation of GAL4 fused human LXR beta expressed in HEK293 cells after 24 hrs by luciferase reporter gene assay B 5.32 pEC50 4750 nM EC50 J Med Chem (2017) 60: 6548-6562 [PMID:28741954]
GtoPdb - - 5.52 pEC50 - - - Nature (1996) 383: 728-31 [PMID:8878485]
RAR-related orphan receptor-γ/Nuclear receptor ROR-gamma in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1741186] [GtoPdb: 600] [UniProtKB: P51449]
ChEMBL Agonist activity at human His6-tagged ROR gamma ligand binding domain (residues 262 to 507) expressed in Escherichia coli BL21 (DE3) cells assessed as increase in recruitment of coactivator peptide by AlphaScreen biochemical assay B 7.7 pEC50 20 nM EC50 Medchemcomm (2013) 4: 764-776
ChEMBL Displacement of [3H]25-hydroxycholesterol from human RORc-LBD expressed in bacterial expression system after 3 hrs by scintillation counting analysis B 7.7 pEC50 20 nM EC50 Bioorg Med Chem Lett (2014) 24: 5769-5776 [PMID:25453817]
ChEMBL Agonist activity at 6xHis tagged human RORgammat LBD (262 to 507 residues) expressed in Escherichia coli BL21 (DE3) assessed as biotinylated SRC1-2 peptide coactivator recruitment by AlphaScreen assay B 7.7 pEC50 20 nM EC50 J Med Chem (2018) 61: 5794-5804 [PMID:29412659]
Farnesoid X receptor in Human [GtoPdb: 603] [UniProtKB: Q96RI1]
GtoPdb - - 5.5 pEC50 - - - J Pharmacol Exp Ther (2006) 317: 317-25 [PMID:16371446]

ChEMBL data shown on this page come from version 32:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]