ChEMBL ligand: CHEMBL566315 (6-ecdca, 6-ethylchenodeoxycholic acid, Acide obeticholique, Acido obeticolico, DSP-1747, INT-747, Obeticholic acid, Ocaliva)
Farnesoid X receptor/Bile acid receptor FXR in Human [ChEMBL: CHEMBL2047] [GtoPdb: 603] [UniProtKB: Q96RI1] There should be some charts here, you may need to enable JavaScript!
GPBA receptor/G-protein coupled bile acid receptor 1 in Human [ChEMBL: CHEMBL5409] [GtoPdb: 37] [UniProtKB: Q8TDU6] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Farnesoid X receptor/Bile acid receptor FXR in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2047] [GtoPdb: 603] [UniProtKB: Q96RI1]
ChEMBL	Agonist activity at FXR (unknown origin) by coactivator recruitment assay	B	4	pEC50	99000	nM	EC50	J Med Chem (2014) 57: 8035-8055 [PMID:25255039]
ChEMBL	Agonist activity at FXR transfected in human HEK293T cells o-expressing pGL3/hBSEP/luc incubated for 24 hrs by Steady-Glo reagent based luciferase reporter gene assay	B	4.38	pEC50	42000	nM	EC50	J Med Chem (2020) 63: 3868-3880 [PMID:31940200]
ChEMBL	Agonist activity at GST-tagged human FXR-LBD (193 to 472 residues) using biotinylated SRC-1 peptide as substrate incubated for 1 hrs by HTRF assay	B	5	pEC50	10000	nM	EC50	J Med Chem (2020) 63: 3868-3880 [PMID:31940200]
ChEMBL	Agonist activity at human FXR expressed in HEK293 cells measured after 18 hrs by steady-glo luciferase reporter gene assay	B	6.15	pEC50	710	nM	EC50	J Med Chem (2020) 63: 12748-12772 [PMID:32991173]
ChEMBL	Agonist activity at Gal4-fused FXR LBD (unknown origin) transfected in HEK293T cells co-transfected with Peak12-Gal4UAS-luci assessed as receptor transactivation incubated for 24 hrs by luciferase reporter gene assay	B	6.17	pEC50	670	nM	EC50	Eur J Med Chem (2023) 252: 115307-115307 [PMID:37003047]
ChEMBL	Agonist activity at Gal4-fused FXR LBD (unknown origin) transfected in HEK293T cells co-transfected with Peak12-Gal4UAS-luciferase reporter assessed as receptor transactivation incubated for 24 hrs by firefly luciferase assay	B	6.17	pEC50	670	nM	EC50	Eur J Med Chem (2023) 245: 114903-114903 [PMID:36375336]
ChEMBL	Transactivation of human FXR (unknown origin) expressed in HepG2 cells co-expressing pSG5RXR/pGL4.70 after 24 hrs post transfection by luciferase reporter gene assay	B	6.3	pEC50	500	nM	EC50	ACS Med Chem Lett (2019) 10: 407-412 [PMID:30996771]
ChEMBL	Agonist activity at human FXR expressed in COS1 cells after 5 hrs by CRE-driven luciferase reporter gene assay	F	6.44	pEC50	361	nM	EC50	J Med Chem (2007) 50: 4265-4268 [PMID:17685603]
ChEMBL	Agonist activity at human FXR	B	6.44	pEC50	360	nM	EC50	Eur J Med Chem (2023) 250: 115143-115143 [PMID:36841086]
ChEMBL	Agonist activity at human FXR expressed in human Huh-7 cells co-transfected with FXRE-Luc reporter plasmid assessed as receptor transactivation incubated for 16 hrs by luciferase reporter gene assay	B	6.49	pEC50	324.1	nM	EC50	Bioorg Med Chem (2021) 43: 116280-116280 [PMID:34256254]
ChEMBL	Agonist activity at GST-tagged human FXR LBD assessed as induction of biotinylated coactivator SRC-1 peptide recruitment measured after 2 hrs by streptavidin-conjugated AlphaScreen assay	B	6.54	pEC50	290	nM	EC50	J Med Chem (2020) 63: 12748-12772 [PMID:32991173]
ChEMBL	Agonist activity at human GST-fused FXR LBD assessed as coactivator interaction with receptor ligand binding domain by Alphascreen assay	B	6.7	pEC50	200	nM	EC50	Bioorg Med Chem (2011) 19: 2650-2658 [PMID:21459580]
ChEMBL	Agonist activity at recombinant human GST-tagged FXR LBD (193 to 472 residues) expressed in baculovirus-infected insect cells assessed as recruitment of biotinylated SRC1 peptide measured after 1 hr by Alphascreen assay	B	6.74	pEC50	180	nM	EC50	ACS Med Chem Lett (2017) 8: 1246-1251 [PMID:29259742]
ChEMBL	Agonist activity at human FXR expressed in human HeLa cells assessed as receptor activation by BSEP promoter-driven firefly luciferase reporter gene assay	B	6.8	pEC50	160	nM	EC50	J Med Chem (2014) 57: 8035-8055 [PMID:25255039]
ChEMBL	Agonist activity at human full length FXR expressed in HeLa cells cotransfected with pSG5-human RXR after 24 hrs by Dual-Glo luciferase reporter gene assay	B	6.8	pEC50	160	nM	EC50	Bioorg Med Chem (2015) 23: 3490-3498 [PMID:25934227]
ChEMBL	Agonist activity at glutathione transferase-tagged human FXR ligand binding domain assessed as biotinylated Src1 peptide recruitment incubated for 30 mins by AlphaScreen assay	B	6.82	pEC50	150	nM	EC50	Eur J Med Chem (2022) 242: 114652-114652 [PMID:36049273]
ChEMBL	Agonist activity at glutathione transferase-tagged human FXR-LBD using biotinylated Src-1 peptide incubated for 30 mins by recruitment coactivator assay	B	6.82	pEC50	150	nM	EC50	J Med Chem (2016) 59: 9201-9214 [PMID:27652492]
GtoPdb	-	-	7	pEC50	99	nM	EC50	J Med Chem (2002) 45: 3569-72 [PMID:12166927]
ChEMBL	Agonist activity at FXR (unknown origin) assessed as recruitment of SRC1 peptide to FXR by FRET assay	B	7	pEC50	100	nM	EC50	J Med Chem (2017) 60: 5235-5266 [PMID:28252961]
ChEMBL	Agonist activity at FXR (unknown origin)	B	7	pEC50	99	nM	EC50	J Med Chem (2020) 63: 5031-5073 [PMID:31930920]
ChEMBL	Effective concentration against Farnesoid X receptor (FXR)	B	7	pEC50	99	nM	EC50	J Med Chem (2002) 45: 3569-3572 [PMID:12166927]
ChEMBL	Activation of human farnesoid X receptor	B	7	pEC50	99	nM	EC50	J Med Chem (2005) 48: 5383-5403 [PMID:16107136]
ChEMBL	Agonist activity at FXR expressed in COS1 cells by cell-based bioluminescence assay	F	7	pEC50	99	nM	EC50	J Med Chem (2009) 52: 7958-7961 [PMID:20014870]
ChEMBL	Binding affinity for Farnesoid X Receptor (FXR)	B	7	pEC50	99	nM	EC50	Bioorg Med Chem Lett (2003) 13: 1865-1868 [PMID:12749886]
ChEMBL	Agonist activity at FXR (unknown origin) by fluorescence resonance energy tranfer assay	B	7	pEC50	99	nM	EC50	Eur J Med Chem (2020) 197: 112311-112311 [PMID:32339855]
ChEMBL	Agonist activity at FXR (unknown origin) by FRET assay	B	7	pEC50	99	nM	EC50	Eur J Med Chem (2022) 243: 114742-114742 [PMID:36155354]
ChEMBL	Binding affinity to FXR assessed as ligand-dependent SRC1 recruitment by FRET based co-activator assay	F	7.01	pEC50	98	nM	EC50	J Med Chem (2006) 49: 4208-4215 [PMID:16821780]
ChEMBL	Binding affinity for human Farnesoid X receptor in FRET assay	B	7.05	pEC50	90	nM	EC50	J Med Chem (2004) 47: 4559-4569 [PMID:15317466]
ChEMBL	Agonist activity at full length FXR in human Huh-7 cells	B	7.07	pEC50	85	nM	EC50	Eur J Med Chem (2020) 197: 112311-112311 [PMID:32339855]
ChEMBL	Agonist activity at FXR (unknown origin) by reporter gene assay	B	7.07	pEC50	85	nM	EC50	J Med Chem (2014) 57: 8035-8055 [PMID:25255039]
ChEMBL	Agonist activity at FXR (unknown origin) by cell based assay	B	7.07	pEC50	85	nM	EC50	J Med Chem (2020) 63: 5031-5073 [PMID:31930920]
ChEMBL	Agonist activity at human FXR expressed in HEK293T cells assessed as BSEP promoter driven cellular transcriptional activity after 24 hrs by luciferase reporter gene assay	B	7.38	pEC50	42	nM	EC50	J Med Chem (2017) 60: 9960-9973 [PMID:29148806]
ChEMBL	Agonist activity at recombinant human GST-tagged FXR ligand binding domain (193 to 472 residues) expressed in baculovirus infected insect cells assessed as induction of interaction with biotin labelled SRC-1 after 1 hr by HTRF assay	B	8	pEC50	10	nM	EC50	J Med Chem (2017) 60: 9960-9973 [PMID:29148806]
GPBA receptor/G-protein coupled bile acid receptor 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5409] [GtoPdb: 37] [UniProtKB: Q8TDU6]
ChEMBL	Agonist activity at human TGR5 receptor expressed in NCI-H716 cells assessed as intracellular cAMP level by TR-FRET assay	F	4.7	pEC50	20000	nM	EC50	Bioorg Med Chem (2011) 19: 2650-2658 [PMID:21459580]
ChEMBL	Agonist activity at TGR5 in human NCI-H716 cells assessed as stimulation of intracellular cAMP accumulation incubated for 60 mins by HTR-FRET assay	F	4.82	pEC50	15000	nM	EC50	J Med Chem (2016) 59: 9201-9214 [PMID:27652492]
ChEMBL	Agonist activity at TGR5 receptor in human NCI-H716 cells assessed as activation of intracellular cAMP production incubated for 1 hr by HTR-FRET assay	F	4.85	pEC50	14000	nM	EC50	Eur J Med Chem (2022) 242: 114652-114652 [PMID:36049273]
ChEMBL	Agonist activity at human TGR5 expressed in HEK293 cells assessed as increase in intracellular cAMP level after 1 hr by TR-FRET assay	F	6.08	pEC50	840	nM	EC50	ACS Med Chem Lett (2017) 8: 1246-1251 [PMID:29259742]
ChEMBL	Agonist activity at TGR5 (unknown origin)	F	6.12	pEC50	760	nM	EC50	Eur J Med Chem (2023) 250: 115143-115143 [PMID:36841086]
ChEMBL	Agonist activity at human TGR5 expressed in CHO cells after 5 hrs by CRE-driven luciferase reporter gene assay	F	6.12	pEC50	755	nM	EC50	J Med Chem (2007) 50: 4265-4268 [PMID:17685603]
ChEMBL	Agonist activity at wild type TGR5 (unknown origin)	B	7	pEC50	100	nM	EC50	ACS Med Chem Lett (2013) 4: 1158-1162 [PMID:24900622]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]