ChEMBL ligand: CHEMBL43048 (3,4-methylenedioxymetamphetamine, 3,4-methylenedioxymethamphetamine, Ecstasy, Mandy, Mdma, Methylenedioxymetamphetamine, Methylenedioxymethamfetamine, Methylenedioxymethamphetamine, Methylenedioxy Methamphetamine, Midomafetamine, Molly)
DAT/Dopamine transporter in Human [ChEMBL: CHEMBL238] [GtoPdb: 927] [UniProtKB: Q01959] DAT/Dopamine transporter in Rat [ChEMBL: CHEMBL338] [GtoPdb: 927] [UniProtKB: P23977] There should be some charts here, you may need to enable JavaScript!
nicotinic acetylcholine receptor α4 subunit/nicotinic acetylcholine receptor β2 subunit/Neuronal acetylcholine receptor; alpha4/beta2 in Rat [ChEMBL: CHEMBL1907596] [GtoPdb: 465, 472] [UniProtKB: P09483, P12390] There should be some charts here, you may need to enable JavaScript!
NET/Norepinephrine transporter in Human [ChEMBL: CHEMBL222] [GtoPdb: 926] [UniProtKB: P23975] There should be some charts here, you may need to enable JavaScript!
SERT/Serotonin transporter in Human [ChEMBL: CHEMBL228] [GtoPdb: 928] [UniProtKB: P31645] SERT/Serotonin transporter in Rat [ChEMBL: CHEMBL313] [GtoPdb: 928] [UniProtKB: P31652] There should be some charts here, you may need to enable JavaScript!
TA1 receptor/Trace amine-associated receptor 1 in Rat [ChEMBL: CHEMBL3833] [GtoPdb: 364] [UniProtKB: Q923Y9] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
DAT/Dopamine transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL238] [GtoPdb: 927] [UniProtKB: Q01959]
ChEMBL	Inhibition of [3H]dopamine uptake at human DAT expressed in HEK293 cells preincubated for 10 mins prior to substrate addition measured after 4 mins by FLIPR assay	B	6.05	pKi	897	nM	Ki	Bioorg Med Chem Lett (2013) 23: 3411-3415 [PMID:23602445]
ChEMBL	Inhibition of [3H]dopamine uptake at human DAT expressed in HEK293 cells preincubated for 10 mins prior to substrate addition measured after 4 mins by FLIPR assay	B	5.62	pIC50	2398.83	nM	IC50	Bioorg Med Chem Lett (2013) 23: 3411-3415 [PMID:23602445]
ChEMBL	Inhibition of human dopamine transporter	B	5.84	pIC50	1442	nM	IC50	Eur J Med Chem (2009) 44: 4862-4888 [PMID:19717215]
ChEMBL	Binding affinity to human DAT	B	5.84	pIC50	1442	nM	IC50	Bioorg Med Chem (2010) 18: 4009-4031 [PMID:20466553]
ChEMBL	Binding affinity to human DAT expressed in HEK293 cells assessed as induction of [3H]MPP+ release by liquid scintillation counting method	B	6	pEC50	1000	nM	EC50	Bioorg Med Chem (2015) 23: 5574-5579 [PMID:26233799]
DAT/Dopamine transporter in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL338] [GtoPdb: 927] [UniProtKB: P23977]
ChEMBL	Activity at DAT in rat synaptosomes assessed as release of [3H]DA after 5 mins by liquid scintillation counting method	B	6.56	pEC50	278	nM	EC50	J Med Chem (2017) 60: 2605-2628 [PMID:28244748]
ChEMBL	Binding affinity to DAT in Sprague-Dawley rat brain synaptosomes assessed as induction of [3H]MPP+ release by liquid scintillation counting method	B	7.12	pEC50	75	nM	EC50	Bioorg Med Chem (2015) 23: 5574-5579 [PMID:26233799]
nicotinic acetylcholine receptor α4 subunit/nicotinic acetylcholine receptor β2 subunit/Neuronal acetylcholine receptor; alpha4/beta2 in Rat (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL1907596] [GtoPdb: 465, 472] [UniProtKB: P09483, P12390]
ChEMBL	Displacement of [3H]epibatidine from alpha4beta2 nAChR in Sprague-Dawley rat brain cortical membrane after 3 hrs	B	5.84	pKi	1440	nM	Ki	Eur J Med Chem (2014) 81: 35-46 [PMID:24942641]
ChEMBL	Displacement of [3H]epibatidine from alpha4beta2 nAChR in Sprague-Dawley rat brain cortical membrane after 3 hrs by liquid scintillation spectrometric analysis	B	4.52	pIC50	30400	nM	IC50	Eur J Med Chem (2014) 81: 35-46 [PMID:24942641]
NET/Norepinephrine transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL222] [GtoPdb: 926] [UniProtKB: P23975]
ChEMBL	Inhibition of [3H]norepinephrine uptake at human NET expressed in HEK293 cells preincubated for 10 mins prior to substrate addition measured after 4 mins by FLIPR assay	B	6.4	pKi	398	nM	Ki	Bioorg Med Chem Lett (2013) 23: 3411-3415 [PMID:23602445]
ChEMBL	Inhibition of [3H]norepinephrine uptake at human NET expressed in HEK293 cells preincubated for 10 mins prior to substrate addition measured after 4 mins by FLIPR assay	B	5.7	pIC50	1995.26	nM	IC50	Bioorg Med Chem Lett (2013) 23: 3411-3415 [PMID:23602445]
ChEMBL	Inhibition of human noradrenaline transporter	B	6.39	pIC50	405	nM	IC50	Eur J Med Chem (2009) 44: 4862-4888 [PMID:19717215]
ChEMBL	Binding affinity to human NET	B	6.39	pIC50	405	nM	IC50	Bioorg Med Chem (2010) 18: 4009-4031 [PMID:20466553]
ChEMBL	Binding affinity to human NET expressed in HEK293 cells assessed as induction of [3H]MPP+ release by liquid scintillation counting method	B	6	pEC50	1000	nM	EC50	Bioorg Med Chem (2015) 23: 5574-5579 [PMID:26233799]
SERT/Serotonin transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL228] [GtoPdb: 928] [UniProtKB: P31645]
ChEMBL	Inhibition of [3H]5-HT uptake at human SERT expressed in HEK293 cells preincubated for 10 mins prior to substrate addition measured after 4 mins by FLIPR assay	B	6.02	pKi	948	nM	Ki	Bioorg Med Chem Lett (2013) 23: 3411-3415 [PMID:23602445]
ChEMBL	Displacement of [3H]paroxetine from human SERT expressed in HEK293 cells	B	8.69	pKi	2.03	nM	Ki	Bioorg Med Chem (2008) 16: 6364-6370 [PMID:18487050]
ChEMBL	Displacement of [3H]citalopram from human SERT expressed in HEK293 cells	B	9.14	pKi	0.73	nM	Ki	Bioorg Med Chem (2008) 16: 6364-6370 [PMID:18487050]
ChEMBL	Inhibition of [3H]5-HT uptake at human SERT expressed in HEK293 cells preincubated for 10 mins prior to substrate addition measured after 4 mins by FLIPR assay	B	5.56	pIC50	2754.23	nM	IC50	Bioorg Med Chem Lett (2013) 23: 3411-3415 [PMID:23602445]
ChEMBL	Inhibition of [3H]-serotonin reuptake at human SERT expressed in HEK293 cells after 15 to 20 mins by fluorescence neurotransmitter transporter assay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem (2011) 19: 1328-1348 [PMID:21227702]
ChEMBL	Inhibition of [3H]5-HT uptake at SERT	B	6.37	pIC50	425	nM	IC50	Eur J Med Chem (2009) 44: 4862-4888 [PMID:19717215]
ChEMBL	Binding affinity to human SERT	B	6.37	pIC50	425	nM	IC50	Bioorg Med Chem (2010) 18: 4009-4031 [PMID:20466553]
ChEMBL	Induction of human SERT-dependent cytotoxicity in SERT expressing human DG75 cells after 48 hrs by neutral red assay	B	4.49	pEC50	32359.37	nM	EC50	Eur J Med Chem (2009) 44: 4862-4888 [PMID:19717215]
ChEMBL	Binding affinity to human SERT expressed in HEK293 cells assessed as induction of [3H]5-HT release by liquid scintillation counting method	B	6	pEC50	1000	nM	EC50	Bioorg Med Chem (2015) 23: 5574-5579 [PMID:26233799]
SERT/Serotonin transporter in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL313] [GtoPdb: 928] [UniProtKB: P31652]
ChEMBL	Inhibition of [3H]5-HT reuptake at rat SERT expressed in HEK293 cells after 2 mins by liquid scintillation counting	B	5.97	pIC50	1060	nM	IC50	Eur J Med Chem (2009) 44: 4862-4888 [PMID:19717215]
ChEMBL	Activity at SERT in rat synaptosomes assessed as release of [3H]HT after 5 mins by liquid scintillation counting method	B	7.14	pEC50	72	nM	EC50	J Med Chem (2017) 60: 2605-2628 [PMID:28244748]
ChEMBL	Binding affinity to SERT in Sprague-Dawley rat brain synaptosomes assessed as induction of [3H]5-HT release by liquid scintillation counting method	B	7.21	pEC50	61	nM	EC50	Bioorg Med Chem (2015) 23: 5574-5579 [PMID:26233799]
TA1 receptor/Trace amine-associated receptor 1 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3833] [GtoPdb: 364] [UniProtKB: Q923Y9]
ChEMBL	Activation of rat TAAR1 expressed in HEK293 cells assessed as accumulation of [3H]cAMP after 1 hr by competitive binding assay	F	5.77	pEC50	1700	nM	EC50	Bioorg Med Chem (2011) 19: 7044-7048 [PMID:22037049]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]