[3H]cAMP [Ligand Id: 5096] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL316966 (Camp, Cyclic Adenosine Monophosphate, cyclic AMP)
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DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
Rap guanine nucleotide exchange factor 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2029197] [GtoPdb: 1292] [UniProtKB: O95398]
ChEMBL Binding affinity to GST-tagged human EPAC1 CNBD (149 to 318 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by 8-NBD-cAMP based competitive fluorescence assay B 5.35 pKd 4500 nM Kd J Med Chem (2020) 63: 4762-4775 [PMID:32297742]
Rap guanine nucleotide exchange factor 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2029198] [GtoPdb: 1293] [UniProtKB: Q8WZA2]
ChEMBL Competitive inhibition of Epac2 using fluorescent nucleotide analog 8-NBD-cAMP by fluorescence analysis B 4.32 pIC50 48000 nM IC50 Bioorg Med Chem Lett (2012) 22: 4038-4043 [PMID:22607683]
ChEMBL Antagonist activity at recombinant EPAC2 (unknown origin) assessed 8-NBD-cAMP substrate fluorescence intensity by substrate competing assay B 4.4 pIC50 40000 nM IC50 J Med Chem (2013) 56: 952-962 [PMID:23286832]
ChEMBL Inhibition of EPAC2 (unknown origin) B 4.4 pIC50 40000 nM IC50 J Med Chem (2014) 57: 3651-3665 [PMID:24256330]

ChEMBL data shown on this page come from version 32:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]