ChEMBL ligand: CHEMBL283807
Adenosine 5`-monophosphoramidase HINT1 in Human [ChEMBL: CHEMBL5878] [UniProtKB: P49773] There should be some charts here, you may need to enable JavaScript!
Hypoxanthine-guanine phosphoribosyltransferase in Human [ChEMBL: CHEMBL2360] [UniProtKB: P00492] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Adenosine 5`-monophosphoramidase HINT1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5878] [UniProtKB: P49773]
ChEMBL	Binding affinity to full-length human N-terminal His6-tagged Hint1 expressed in Escherichia coli Rosetta 2 pLysS cells by isothermal titration calorimetry	B	4.17	pKd	67000	nM	Kd	US-20180065965-A1. SULFAMIDE AND SULFAMATE INHIBITORS OF hHint1 (2018)
ChEMBL	Isothermal Titration Calorimetry (ITC): ITC experiments were conducted on a MicroCal Auto-ITC200 system (GE Healthcare life sciences). All titration experiments were performed at 20° C. in ITC buffer (10 mM Tris, 150 mM NaCl, pH 7.5). hHint1 was exchanged into ITC buffer using Micro biospin6 columns (BioRad, USA) and final protein concentrations were determined as described above. To determine the dissociation constant of stock concentration (300-400 uM) of inhibitors was titrated with 15-20 uM of Hint1. Twenty injections of ligand were injected (injection volume 2 ul) into the protein cell. The resulting change in enthalpy was measured and the background heat of dilution was subtracted by performing similar experiments in the absence of inhibitors. The background heat of dilution was subtracted from the resulting data and was fitted into one-site binding model using the ITC200 microcal software. The resulting association constant obtained by fitting the curve was converted into Kd using Ka=1/Kd relationship.	B	4.17	pKd	67000	nM	Kd	US-10513520-B2. Sulfamide and sulfamate inhibitors of hHint1 (2019)
Hypoxanthine-guanine phosphoribosyltransferase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2360] [UniProtKB: P00492]
ChEMBL	Inhibition of human recombinant HGPRT at pH 7.4 by spectrophotometric assay	B	5.24	pKi	5800	nM	Ki	J Med Chem (2009) 52: 4391-4399 [PMID:19527031]
ChEMBL	Inhibition of human HGPRT	B	5.24	pKi	5800	nM	Ki	J Med Chem (2015) 58: 4822-4838 [PMID:25915781]
ChEMBL	Binding affinity to human HGPRT assessed as inhibition constant	B	5.24	pKi	5800	nM	Ki	J Med Chem (2024) 67: 7158-7175 [PMID:38651522]

ChEMBL data shown on this page come from version 36:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]