ChEMBL ligand: CHEMBL1534 (Bisulase, C.i. 50900, C.i. food yellow 15, E101, E-101(iii), E-b2, Flavaxin, Freeda, Ins-101(iii), INS NO. 101(I), Ins no.101(iii), Lacto-flavin, Lactoflavin, NCI-0033298, NSC-33298, Riboflavin, Riboflavine, Riboflavin tetrabutyrate, Riboflavinum, Riboflavin (vit b2), Russupteridine yellow iii, Vitamin b-2, Vitamin b2, Vitamin-b2, Vitamin B 2, Vitamin b2 (as riboflavin), Vitamin g, Vitamin G)
GroEL/GroES in Escherichia coli [ChEMBL: CHEMBL4106139] [UniProtKB: Q548M1, Q7BGE6] There should be some charts here, you may need to enable JavaScript!
HSP60/HSP10 in Human [ChEMBL: CHEMBL4106131] [UniProtKB: P10809, P61604] There should be some charts here, you may need to enable JavaScript!
Riboflavin-binding protein in Chicken [ChEMBL: CHEMBL1944489] [UniProtKB: P02752] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
GroEL/GroES in Escherichia coli (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4106139] [UniProtKB: Q548M1, Q7BGE6]
ChEMBL	Inhibition of Escherichia coli GroEL expressed in Escherichia coliDH5alpha/Escherichia coli GroES expressed in Escherichia coli BL21 (DE3) assessed as reduction in GroEL/GroES-mediated denatured soluble pig heart MDH refolding by measuring MDH enzyme activity using sodium mesoxalate as substrate after 20 to 40 mins by malachite green dye based spectrometric analysis	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem Lett (2019) 29: 1106-1112 [PMID:30852084]
ChEMBL	Inhibition of Escherichia coli GroEL expressed in Escherichia coli DH5alpha/Escherichia coli GroES expressed in Escherichia coli BL21 (DE3) assessed as reduction in GroEL/GroES-mediated denatured rhodanese refolding by measuring rhodanese enzyme activity after 45 mins by Fe(SCN)3 dye based spectrometric analysis	B	4.74	pIC50	18000	nM	IC50	Bioorg Med Chem Lett (2019) 29: 1106-1112 [PMID:30852084]
HSP60/HSP10 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4106131] [UniProtKB: P10809, P61604]
ChEMBL	Inhibition of human N-terminal octa-His-tagged HSP60 expressed in Escherichia coli Rosetta(DE3) pLysS/human HSP10 expressed in Escherichia coli Rosetta(DE3) assessed as reduction in HSP60/HSP10-mediated denatured MDH refolding by measuring MDH enzyme activity using sodium mesoxalate as substrate after 40 to 60 mins by malachite green dye based spectrometric analysis	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem Lett (2019) 29: 1106-1112 [PMID:30852084]
Riboflavin-binding protein in Chicken (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1944489] [UniProtKB: P02752]
ChEMBL	Binding affinity to chicken riboflavin binding protein assessed as dissociation constant at pH 4 in 0.1 M NaAc by isothermal titration calorimetric assay	B	7.34	pKd	46	nM	Kd	ACS Med Chem Lett (2011) 2: 363-367 [PMID:21686082]
ChEMBL	Binding affinity to chicken riboflavin binding protein assessed as dissociation constant at pH 9 in 0.1 M Tris by isothermal titration calorimetric assay	B	7.47	pKd	34	nM	Kd	ACS Med Chem Lett (2011) 2: 363-367 [PMID:21686082]
ChEMBL	Binding affinity to chicken riboflavin binding protein assessed as dissociation constant at pH 7.4 in 0.1 M phosphate buffer by isothermal titration calorimetric assay	B	8.18	pKd	6.6	nM	Kd	ACS Med Chem Lett (2011) 2: 363-367 [PMID:21686082]
ChEMBL	Binding affinity to chicken riboflavin binding protein assessed as dissociation constant at pH 7.4 in phosphate buffer by isothermal titration calorimetric assay	B	8.3	pKd	5	nM	Kd	ACS Med Chem Lett (2011) 2: 363-367 [PMID:21686082]
ChEMBL	Competitive inhibition of chicken riboflavin binding protein by surface plasmon resonance assay	B	6.46	pKi	350	nM	Ki	ACS Med Chem Lett (2011) 2: 363-367 [PMID:21686082]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]