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ChEMBL ligand: CHEMBL887 (AGN-1135, Azilect, NSC-759639, Rasagiline, TV-1030) |
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DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
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acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL220] [GtoPdb: 2465] [UniProtKB: P22303] | ||||||||
ChEMBL | Inhibition of human erythrocyte AChE using acetylthiocholine chloride as substrate incubated for 15 mins by Ellman's method | B | 7.72 | pIC50 | 19 | nM | IC50 | Bioorg Med Chem Lett (2017) 27: 5053-5059 [PMID:29033232] |
butyrylcholinesterase/Butyrylcholinesterase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1914] [GtoPdb: 2471] [UniProtKB: P06276] | ||||||||
ChEMBL | Inhibition of human serum BChE using butyrylthiocholine chloride as substrate incubated for 15 mins by Ellman's method | B | 5.32 | pIC50 | 4760 | nM | IC50 | Bioorg Med Chem Lett (2017) 27: 5053-5059 [PMID:29033232] |
Monoamine oxidase A in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1951] [GtoPdb: 2489] [UniProtKB: P21397] | ||||||||
ChEMBL | Binding affinity towards monoamine oxidase A activity was measured using a kynuramine assay | B | 5.01 | pKi | 9700 | nM | Ki | J Med Chem (2004) 47: 1760-1766 [PMID:15027867] |
ChEMBL | Inhibition of human recombinant microsomal MAOA expressed in baculovirus infected BTI-TN-5B1- 4 cells using p-tyramine as substrate assessed as decrease in H2O2 production by amplex red-based fluorescence assay | B | 4.28 | pIC50 | 53000 | nM | IC50 | Eur J Med Chem (2018) 158: 781-800 [PMID:30245401] |
ChEMBL | Inhibition of human microsomal MAO-A expressed in recombinant baculovirus infected insect BTI-TN-5B1-4 cells assessed as reduction in H2O2 production using p-tyramine as substrate after 15 mins by fluorescence assay | B | 4.28 | pIC50 | 52974 | nM | IC50 | J Med Chem (2016) 59: 5879-5893 [PMID:27244485] |
ChEMBL | Inhibition of human recombinant microsomal MAOA expressed in baculovirus infected BTI-TN-5B1- 4 cells using p-tyramine as substrate assessed as decrease in H2O2 production after 15 mins by amplex red-based fluorescence assay | B | 4.28 | pIC50 | 52974 | nM | IC50 | J Med Chem (2017) 60: 7206-7212 [PMID:28753307] |
ChEMBL | Inhibition of recombinant human MAO-A using p-tyramine as substrate assessed as decrease in H2O2 production incubated for 15 mins by fluorimetric method | B | 4.29 | pIC50 | 50710 | nM | IC50 | Bioorg Med Chem (2016) 24: 5929-5940 [PMID:27692996] |
ChEMBL | Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI insect cells using p-tyramine as substrate pretreated for 15 mins followed by substrate addition after 20 mins by Amplex red reagent based fluorimetric method | B | 4.3 | pIC50 | 49700 | nM | IC50 | Bioorg Med Chem (2017) 25: 3815-3826 [PMID:28549891] |
ChEMBL | Inhibition of recombinant human microsomal MAOA expressed in baculovirus infected BTI insect cells using p-tyramine as substrate preincubated for 15 mins followed by substrate addition and measured over 20 mins by amplex red reagent-based horseradish peroxidase-coupled fluorometric assay | B | 4.53 | pIC50 | 29520 | nM | IC50 | J Med Chem (2020) 63: 1361-1387 [PMID:31917923] |
ChEMBL | Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI-TN-5B1-4 insect cells using p-tyramine as substrate preincubated for 15 mins followed by substrate addition and measured over 20 mins by horse-radish peroxidase/Amplex Red coupled fluorimetric analysis | B | 4.53 | pIC50 | 29500 | nM | IC50 | Eur J Med Chem (2021) 209: 112911-112911 [PMID:33071056] |
ChEMBL | Inhibition of human recombinant microsomal MAO-A expressed in baculovirus infected BTI-TN-5B1-4 cells using p-tyramine as substrate assessed as production of H2O2 incubated for 15 mins followed by substrate addition measured over 15 mins by fluorimetric analysis | B | 4.78 | pIC50 | 16440 | nM | IC50 | Eur J Med Chem (2013) 63: 151-161 [PMID:23474901] |
ChEMBL | Inhibition of human MAO-A assessed as inhibition of H2O2 production using p-tyramine as substrate | B | 4.85 | pIC50 | 14200 | nM | IC50 | Bioorg Med Chem Lett (2022) 74: 128917-128917 [PMID:35926797] |
ChEMBL | Inhibition of human microsomal MAO-A expressed in baculovirus infected BTI-TN-5B1-4 cells assessed as reduction in 4-hydroxyquinoline formation using kynuramine as substrate preincubated with substrate for 10 mins followed by enzyme addition by spectrophotometric analysis | B | 5.44 | pIC50 | 3650 | nM | IC50 | Eur J Med Chem (2020) 185: 111770-111770 [PMID:31711793] |
ChEMBL | Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI insect cells using kynuramine as substrate after 30 mins by fluorimetric method | B | 5.58 | pIC50 | 2610 | nM | IC50 | Bioorg Med Chem (2017) 25: 1997-2009 [PMID:28237559] |
ChEMBL | Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI-TN- 5B1-4 insect cells using kynuramine as substrate preincubated for 10 mins in presence of substrate followed by enzyme addition and measured every minute for 30 mins by spectrophotometry analysis | B | 5.63 | pIC50 | 2340 | nM | IC50 | J Med Chem (2021) 64: 11169-11182 [PMID:34269579] |
ChEMBL | Inhibition of recombinant human MAO-A using kynuramine as substrate after 30 mins by fluorescence method | B | 5.67 | pIC50 | 2130 | nM | IC50 | Bioorg Med Chem Lett (2017) 27: 5046-5052 [PMID:29033233] |
ChEMBL | Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI insect cells using kynuramine as substrate after 30 mins by fluorescence assay | B | 5.67 | pIC50 | 2130 | nM | IC50 | Bioorg Med Chem (2017) 25: 3006-3017 [PMID:28487125] |
ChEMBL | Inhibition of human recombinant MAO-A expressed in baculovirus infected BTI insect cells using kynuramine as substrate after 30 mins by fluorescence assay | B | 5.81 | pIC50 | 1560 | nM | IC50 | Bioorg Med Chem Lett (2017) 27: 5053-5059 [PMID:29033232] |
ChEMBL | Inhibition of human recombinant MAO-A using kynuramine as substrate measured after 30 mins by fluorescence assay | B | 5.85 | pIC50 | 1420 | nM | IC50 | Bioorg Med Chem (2017) 25: 714-726 [PMID:27923535] |
ChEMBL | Inhibition of human recombinant MAO-A using kynuramine as substrate after 30 mins by fluorescence spectrophotometry | B | 5.85 | pIC50 | 1420 | nM | IC50 | Bioorg Med Chem (2016) 24: 2342-2351 [PMID:27079124] |
ChEMBL | Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI insect cells using kynuramine as substrate incubated for 30 mins by fluorescence assay | B | 5.85 | pIC50 | 1420 | nM | IC50 | Eur J Med Chem (2017) 126: 762-775 [PMID:27951485] |
ChEMBL | Inhibition of recombinant human MAO-A expressed in baculovirus infected High 5 insect cells using kynuramine as substrate incubated for 30 mins by spectrofluorometric method | B | 5.85 | pIC50 | 1420 | nM | IC50 | Bioorg Med Chem (2017) 25: 1030-1041 [PMID:28011206] |
ChEMBL | Inhibition of recombinant human MAO-A using kynuramine as substrate after 30 mins by fluorescence assay | B | 5.85 | pIC50 | 1420 | nM | IC50 | Bioorg Med Chem (2018) 26: 1885-1895 [PMID:29500132] |
ChEMBL | Inhibition of recombinant human MAOA expressed in baculovirus infected in BTI cells using kynuramine as substrate after 30 mins by fluorescence based assay | B | 5.99 | pIC50 | 1020 | nM | IC50 | Bioorg Med Chem (2020) 28: 115374-115374 [PMID:32089390] |
ChEMBL | Inhibition of recombinant human MAO-A using p-tyramine as substrate preincubated for 15 mins followed by substrate addition measured over 15 mins by Amplex red reagent-based fluorimetric method | B | 6 | pIC50 | 1010 | nM | IC50 | Eur J Med Chem (2018) 145: 588-593 [PMID:29339253] |
ChEMBL | Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI insect cells using p-tyramine as substrate preincubated for 60 mins followed by substrate addition and measured after 30 mins by horse-radish peroxidase-coupled amplex red reagent-based fluorescence spectrophotometric method | B | 6.12 | pIC50 | 750 | nM | IC50 | Eur J Med Chem (2019) 173: 203-212 [PMID:31005056] |
ChEMBL | Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI insect cells using kynuramine as substrate measured after 30 mins by fluorescence based assay | B | 6.15 | pIC50 | 712 | nM | IC50 | Bioorg Med Chem (2020) 28: 115400-115400 [PMID:32146060] |
ChEMBL | Irreversible inhibition of human cerebral cortex MAO-A using [14C]-5-hydroxytryptamine creatinine disulphate as substrate pretreated for 60 mins followed by substrate addition after 30 mins by liquid scintillation counting method | B | 6.15 | pIC50 | 710 | nM | IC50 | Eur J Med Chem (2017) 130: 365-378 [PMID:28273563] |
ChEMBL | Inhibition of recombinant human MAO-A using kynuramine as substrate after 30 mins by fluorescence assay | B | 6.15 | pIC50 | 710 | nM | IC50 | Bioorg Med Chem Lett (2017) 27: 718-722 [PMID:28131710] |
ChEMBL | Inhibitory concentration towards in human monoamine oxidase A was measured | B | 6.15 | pIC50 | 700 | nM | IC50 | J Med Chem (2004) 47: 1760-1766 [PMID:15027867] |
ChEMBL | Inhibition of human recombinant MAOA assessed as H2O2 production by Amplex Red reagent-based assay | B | 6.15 | pIC50 | 700 | nM | IC50 | J Med Chem (2012) 55: 8483-8492 [PMID:22978824] |
ChEMBL | Inhibition of human recombinant MAO-A expressed in baculovirus infected BTI-TN-5B1-4 insect cells assessed as decrease in H2O2 production using p-tyramine as substrate incubated for 20 mins by horse-radish peroxidase/amplex red-based fluorescence method | B | 6.17 | pIC50 | 680 | nM | IC50 | Eur J Med Chem (2019) 179: 404-422 [PMID:31265934] |
ChEMBL | Inhibition of human recombinant MAO-A expressed in baculovirus infected BTI-TN-5B1-4 insect cells using p-tyramine as substrate preincubated for 15 mins and measured after 45 mins by resorufin-based fluorescence assay | B | 6.17 | pIC50 | 680 | nM | IC50 | Eur J Med Chem (2019) 162: 793-809 [PMID:30522087] |
ChEMBL | Inhibition of human recombinant MAO-A using kynuramine as substrate measured after 30 mins by fluorimetric assay | B | 6.2 | pIC50 | 630 | nM | IC50 | Bioorg Med Chem Lett (2019) 29: 126625-126625 [PMID:31444085] |
ChEMBL | Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI insect cells using kynuramine as substrate measured after 30 mins by fluorescence based assay | B | 6.21 | pIC50 | 620 | nM | IC50 | Eur J Med Chem (2019) 178: 726-739 [PMID:31229875] |
ChEMBL | Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI insect cells using kynuramine as substrate after 30 mins by fluorescence based assay | B | 6.23 | pIC50 | 587 | nM | IC50 | Eur J Med Chem (2019) 183: 111737-111737 [PMID:31581002] |
ChEMBL | Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI insect cells using kynuramine as substrate incubated for 30 mins by fluorescence based assay | B | 6.23 | pIC50 | 587 | nM | IC50 | Eur J Med Chem (2019) 180: 238-252 [PMID:31310916] |
ChEMBL | Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI insect cells using kynuramine as substrate after 30 mins by fluorescence assay | B | 6.23 | pIC50 | 587 | nM | IC50 | Bioorg Med Chem (2018) 26: 1102-1115 [PMID:29409707] |
ChEMBL | Inhibition of human recombinant MAO-A by multimode plate reader assay | B | 6.23 | pIC50 | 587 | nM | IC50 | Eur J Med Chem (2021) 216: 113310-113310 [PMID:33667847] |
ChEMBL | Inhibition Assay: MAO-A and MAO-B activity was measured using radioactive substrates. The substrate for MAO-A was 5 HT and for MAO-B was PEA. When measuring the activity of MAO-A, the MAO-B activity was inhibited with deprenyl and when measuring the activity of MAO-B the activity of MAO-A was inhibited with clorgylin. Blank samples were produced using TCP to inhibit both of the enzymes. The metabolites were extracted to toluene and read in a β -counter. The results are expressed in relative activity and normalized to the amount of protein in the tissue. Figs. 1 and 2 show MAO-A/MAO-B activity of compounds 3, 4 and of 0-Methyl-M3O at various concentrations (10 -"5 -10 -"8 ). As presented in Figs. 1 and 2, it can be seen that compounds 3, 4 and 0-Methyl-M3O were all potent inhibitors of MAO-A and MAO-B extracted from rat brain, compound 3 clearly the most potent inhibitor of the three compounds. | B | 6.39 | pIC50 | 410 | nM | IC50 | US-9034303-B2. Neuroprotective and neuro-restorative iron chelators and monoamine oxidase inhibitors and uses thereof (2015) |
ChEMBL | Inhibition of MAOA | B | 6.39 | pIC50 | 410 | nM | IC50 | J Med Chem (2008) 51: 347-372 [PMID:18181565] |
ChEMBL | Inhibition of recombinant human MAO-A | B | 7.49 | pIC50 | 32 | nM | IC50 | Eur J Med Chem (2020) 194: 112265-112265 [PMID:32240904] |
Monoamine oxidase A in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3358] [UniProtKB: P21396] | ||||||||
ChEMBL | Inhibition of Sprague-Dawley rat liver MAO-A using p-tyramine as substrate preincubated for 15 mins and measured after 45 mins by resorufin-based fluorescence assay | B | 6.37 | pIC50 | 422 | nM | IC50 | Eur J Med Chem (2019) 162: 793-809 [PMID:30522087] |
ChEMBL | In vitro inhibitory concentration against Monoamine oxidase A of rat brain homogenates; value ranges from 0.28-0.54 | B | 6.39 | pIC50 | 410 | nM | IC50 | J Med Chem (2002) 45: 5260-5279 [PMID:12431053] |
ChEMBL | Inhibition of MAO-A in rat brain using [14C]-5-hydroxytryptamine creatinine disulfate as substrate preincubated for 60 mins followed by substrate addition and measured after 30 mins by liquid scintillation counting method | B | 6.39 | pIC50 | 410 | nM | IC50 | J Med Chem (2019) 62: 8881-8914 [PMID:31082225] |
ChEMBL | Inhibition of rat brain MAO-A in nuclei-free homogenates using [14C]hydroxytryptamine substrate after 20 mins by liquid scintillation counting | B | 9 | pIC50 | 1 | nM | IC50 | J Med Chem (2015) 58: 1400-1419 [PMID:25627172] |
Monoamine oxidase B in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2039] [GtoPdb: 2490] [UniProtKB: P27338] | ||||||||
ChEMBL | Binding affinity towards monoamine oxidase B activity was measured using a benzylamine assay | B | 6.15 | pKi | 700 | nM | Ki | J Med Chem (2004) 47: 1760-1766 [PMID:15027867] |
ChEMBL | Inhibition constant against human recombinant Monoamine oxidase-B | B | 6.15 | pKi | 700 | nM | Ki | Bioorg Med Chem Lett (2005) 15: 4438-4446 [PMID:16137882] |
ChEMBL | Binding affinity to human recombinant MAOB | B | 6.15 | pKi | 700 | nM | Ki | Medchemcomm (2011) 2: 1099-1103 |
ChEMBL | Inhibition of human recombinant microsomal MAO-B expressed in Pichia pastoris incubated for 30 mins prior to substrate addition measured after 60 mins by MAO-Glo assay | B | 7.85 | pKi | 14.2 | nM | Ki | Bioorg Med Chem (2015) 23: 770-778 [PMID:25600407] |
ChEMBL | Inhibition of human recombinant soluble MAO-B expressed in Pichia pastoris incubated for 30 mins prior to substrate addition measured after 60 mins by MAO-Glo assay | B | 8.12 | pKi | 7.6 | nM | Ki | Bioorg Med Chem (2015) 23: 770-778 [PMID:25600407] |
ChEMBL | Inhibition of recombinant human MAOB using kynuramine as substrate measured for 30 mins by fluorescence spectrophotometric assay | B | 4.34 | pIC50 | 45700 | nM | IC50 | Eur J Med Chem (2021) 213: 113183-113183 [PMID:33493825] |
ChEMBL | Inhibition of recombinant human MAOB expressed in baculovirus infected BTI insect cells using p-tyramine as substrate preincubated for 30 mins followed by substrate addition and measured over 45 mins by horseradish peroxidase-Amplex Red-coupled fluorometric assay | B | 4.81 | pIC50 | 15400 | nM | IC50 | Bioorg Med Chem (2019) 27: 1195-1210 [PMID:30808606] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using p-tyramine as substrate pretreated for 15 mins followed by substrate addition after 20 mins by Amplex red reagent based fluorimetric method | B | 5.13 | pIC50 | 7470 | nM | IC50 | Bioorg Med Chem (2017) 25: 3815-3826 [PMID:28549891] |
ChEMBL | Inhibition of recombinant human MAO-B | B | 6.28 | pIC50 | 530 | nM | IC50 | Eur J Med Chem (2020) 194: 112265-112265 [PMID:32240904] |
ChEMBL | Inhibition of recombinant human MAOB expressed in baculovirus infected BTI insect cells using p-tyramine as substrate measured after 15 mins by Amplex red reagent based fluorescence assay | B | 6.85 | pIC50 | 141.7 | nM | IC50 | Eur J Med Chem (2020) 202: 112475-112475 [PMID:32652406] |
ChEMBL | Inhibition of human recombinant MAO-B expressed in baculovirus infected BTI insect cells using kynuramine as substrate after 30 mins by fluorescence assay | B | 7.06 | pIC50 | 87.6 | nM | IC50 | Bioorg Med Chem Lett (2017) 27: 5053-5059 [PMID:29033232] |
ChEMBL | Inhibition of recombinant human MAO-B using kynuramine as substrate after 30 mins by fluorescence method | B | 7.07 | pIC50 | 86 | nM | IC50 | Bioorg Med Chem Lett (2017) 27: 5046-5052 [PMID:29033233] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using kynuramine as substrate after 30 mins by fluorescence assay | B | 7.07 | pIC50 | 86 | nM | IC50 | Bioorg Med Chem (2017) 25: 3006-3017 [PMID:28487125] |
ChEMBL | Inhibition of recombinant human MAO-B using kynuramine as substrate after 30 mins by fluorescence assay | B | 7.08 | pIC50 | 83 | nM | IC50 | Bioorg Med Chem (2018) 26: 1885-1895 [PMID:29500132] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected High 5 insect cells using kynuramine as substrate incubated for 30 mins by spectrofluorometric method | B | 7.08 | pIC50 | 83 | nM | IC50 | Bioorg Med Chem (2017) 25: 1030-1041 [PMID:28011206] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using kynuramine as substrate incubated for 30 mins by fluorescence assay | B | 7.08 | pIC50 | 82.5 | nM | IC50 | Eur J Med Chem (2017) 126: 762-775 [PMID:27951485] |
ChEMBL | Inhibition of human recombinant MAO-B using kynuramine as substrate after 30 mins by fluorescence spectrophotometry | B | 7.08 | pIC50 | 82.5 | nM | IC50 | Bioorg Med Chem (2016) 24: 2342-2351 [PMID:27079124] |
ChEMBL | Inhibition of human recombinant MAO-B using kynuramine as substrate measured after 30 mins by fluorescence assay | B | 7.08 | pIC50 | 82.5 | nM | IC50 | Bioorg Med Chem (2017) 25: 714-726 [PMID:27923535] |
ChEMBL | Inhibition of human MAO-B assessed as inhibition of H2O2 production using p-tyramine as substrate | B | 7.15 | pIC50 | 70 | nM | IC50 | Bioorg Med Chem Lett (2022) 74: 128917-128917 [PMID:35926797] |
ChEMBL | Inhibition of human recombinant microsomal MAO-B expressed in baculovirus infected BTI-TN-5B1-4 cells using p-tyramine as substrate assessed as production of H2O2 incubated for 15 mins followed by substrate addition measured over 15 mins by fluorimetric analysis | B | 7.16 | pIC50 | 69 | nM | IC50 | Eur J Med Chem (2013) 63: 151-161 [PMID:23474901] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using tyramine as substrate pretreated for 30 mins followed by substrate addition incubated for 30 mins measured at 5 mins interval by horse-radish peroxidase/amplex red-based fluorometric method | B | 7.18 | pIC50 | 66 | nM | IC50 | Eur J Med Chem (2017) 131: 92-106 [PMID:28301816] |
ChEMBL | Inhibition of human microsomal MAO-B expressed in recombinant baculovirus infected insect BTI-TN-5B1-4 cells assessed as reduction in H2O2 production using p-tyramine as substrate after 15 mins by fluorescence assay | B | 7.3 | pIC50 | 50.12 | nM | IC50 | J Med Chem (2016) 59: 5879-5893 [PMID:27244485] |
ChEMBL | Inhibition of human recombinant MAOB expressed in baculovirus infected BTI-TN-5B1- 4 cells using p-tyramine as substrate assessed as decrease in H2O2 production by amplex red-based fluorescence assay | B | 7.3 | pIC50 | 50 | nM | IC50 | Eur J Med Chem (2018) 158: 781-800 [PMID:30245401] |
ChEMBL | Inhibition of human recombinant microsomal MAOB expressed in baculovirus infected BTI-TN-5B1- 4 cells using p-tyramine as substrate assessed as decrease in H2O2 production after 15 mins by amplex red-based fluorescence assay | B | 7.3 | pIC50 | 49.66 | nM | IC50 | J Med Chem (2017) 60: 7206-7212 [PMID:28753307] |
ChEMBL | Inhibition of human microsomal MAO-B expressed in recombinant baculovirus infected insect BTI-TN-5B1-4 cells assessed as reduction in H2O2 production using p-tyramine as substrate after 15 mins by fluorescence assay | B | 7.3 | pIC50 | 49.66 | nM | IC50 | J Med Chem (2016) 59: 5879-5893 [PMID:27244485] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in insect cells using kynuramine as substrate assessed as formation of 4-hydroxyquinoline measured every 5 mins for 30 mins | B | 7.34 | pIC50 | 46 | nM | IC50 | Bioorg Med Chem (2013) 21: 6634-6641 [PMID:24012376] |
ChEMBL | Inhibition of human recombinant MAO-B expressed in insect cells assessed as kynuramine hydrobromide oxidation after 20 mins by spectrophotometric analysis | B | 7.34 | pIC50 | 46 | nM | IC50 | Bioorg Med Chem (2012) 20: 3065-3071 [PMID:22436387] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI-TN- 5B1-4 insect cells using kynuramine as substrate preincubated for 10 mins in presence of substrate followed by enzyme addition and measured every minute for 30 mins by spectrophotometry analysis | B | 7.34 | pIC50 | 45.7 | nM | IC50 | J Med Chem (2021) 64: 11169-11182 [PMID:34269579] |
ChEMBL | Inhibition of MAO-B (unknown origin) incubated for 30 mins | B | 7.36 | pIC50 | 44 | nM | IC50 | Eur J Med Chem (2022) 233: 114242-114242 [PMID:35276424] |
ChEMBL | Inhibition of recombinant human MAO-B using kynuramine as substrate after 30 mins by fluorescence assay | B | 7.36 | pIC50 | 44 | nM | IC50 | Bioorg Med Chem Lett (2017) 27: 718-722 [PMID:28131710] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using kynuramine as substrate measured after 30 mins by fluorescence based assay | B | 7.36 | pIC50 | 43.7 | nM | IC50 | Bioorg Med Chem (2020) 28: 115400-115400 [PMID:32146060] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI-TN-5B1-4 insect cells using p-tyramine as substrate preincubated for 15 mins followed by substrate addition and measured over 20 mins by horse-radish peroxidase/Amplex Red coupled fluorimetric analysis | B | 7.44 | pIC50 | 36 | nM | IC50 | Eur J Med Chem (2021) 209: 112911-112911 [PMID:33071056] |
ChEMBL | Inhibition of recombinant human microsomal MAOB expressed in baculovirus infected BTI insect cells using p-tyramine as substrate preincubated for 15 mins followed by substrate addition and measured over 20 mins by amplex red reagent-based horseradish peroxidase-coupled fluorometric assay | B | 7.44 | pIC50 | 36 | nM | IC50 | J Med Chem (2020) 63: 1361-1387 [PMID:31917923] |
ChEMBL | Inhibition of human recombinant MAO-B using kynuramine as substrate measured after 30 mins by fluorimetric assay | B | 7.44 | pIC50 | 36 | nM | IC50 | Bioorg Med Chem Lett (2019) 29: 126625-126625 [PMID:31444085] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using kynuramine as substrate measured after 30 mins by fluorescence based assay | B | 7.51 | pIC50 | 31 | nM | IC50 | Eur J Med Chem (2019) 178: 726-739 [PMID:31229875] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using kynuramine as substrate after 30 mins by fluorescence assay | B | 7.55 | pIC50 | 28.1 | nM | IC50 | Bioorg Med Chem (2018) 26: 1102-1115 [PMID:29409707] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using kynuramine as substrate after 30 mins by fluorescence based assay | B | 7.55 | pIC50 | 28.1 | nM | IC50 | Eur J Med Chem (2019) 183: 111737-111737 [PMID:31581002] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using kynuramine as substrate incubated for 30 mins by fluorescence based assay | B | 7.55 | pIC50 | 28.1 | nM | IC50 | Eur J Med Chem (2019) 180: 238-252 [PMID:31310916] |
ChEMBL | Inhibition of human recombinant MAO-B by multimode plate reader assay | B | 7.55 | pIC50 | 28.1 | nM | IC50 | Eur J Med Chem (2021) 216: 113310-113310 [PMID:33667847] |
ChEMBL | Inhibition of human MAO-B expressed in baculovirus infected BTI insect cells using p-tyramine as substrate preincubated for 30 mins followed by substrate addition and measured after 1 hr by fluorescence-based Amplex Red MAO assay | B | 7.6 | pIC50 | 25 | nM | IC50 | Bioorg Med Chem Lett (2018) 28: 3596-3600 [PMID:30404719] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI cells using p-tyramine as substrate by fluorometric assay | B | 7.6 | pIC50 | 25 | nM | IC50 | Eur J Med Chem (2020) 207: 112743-112743 [PMID:32882609] |
ChEMBL | Inhibition of recombinant human MAOB using kynuramine as substrate preincubated for 30 mins followed by substrate addition and measured after 30 mins by fluorescence assay | B | 7.64 | pIC50 | 23 | nM | IC50 | Bioorg Med Chem (2021) 35: 116074-116074 [PMID:33640707] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using p-tyramine as substrate preincubated for 30 mins followed by substrate addition and measured after 1 hr by fluorimetric analysis | B | 7.81 | pIC50 | 15.4 | nM | IC50 | Eur J Med Chem (2020) 208: 112765-112765 [PMID:32949963] |
ChEMBL | Inhibition of human recombinant MAO-B using p-tyramine as substrate preincubated for 30 mins followed by substrate addition and measured after 1 hr by fluorometric analysis | B | 7.81 | pIC50 | 15.4 | nM | IC50 | Eur J Med Chem (2022) 236: 114329-114329 [PMID:35397400] |
GtoPdb | - | - | 7.85 | pIC50 | 14 | nM | IC50 | Br J Pharmacol (2001) 132: 500-6 [PMID:11159700] |
ChEMBL | Inhibitory concentration towards in human monoamine oxidase B was measured | B | 7.85 | pIC50 | 14 | nM | IC50 | J Med Chem (2004) 47: 1760-1766 [PMID:15027867] |
ChEMBL | Inhibition of human recombinant MAOB assessed as H2O2 production by Amplex Red reagent-based assay | B | 7.85 | pIC50 | 14 | nM | IC50 | J Med Chem (2012) 55: 8483-8492 [PMID:22978824] |
ChEMBL | Irreversible inhibition of human recombinant MAOB | B | 7.85 | pIC50 | 14 | nM | IC50 | Medchemcomm (2011) 2: 1099-1103 |
ChEMBL | Irreversible inhibition of human cerebral cortex MAO-B using [14C]-phenylethylamin as substrate pretreated for 60 mins followed by substrate addition after 20 mins by liquid scintillation counting method | B | 7.85 | pIC50 | 14 | nM | IC50 | Eur J Med Chem (2017) 130: 365-378 [PMID:28273563] |
ChEMBL | Irreversible inhibition of human MAOB | B | 7.85 | pIC50 | 14 | nM | IC50 | Bioorg Med Chem Lett (2018) 28: 3596-3600 [PMID:30404719] |
ChEMBL | Inhibition of human recombinant MAO-B expressed in baculovirus infected BTI-TN-5B1-4 insect cells using p-tyramine as substrate preincubated for 15 mins and measured after 45 mins by resorufin-based fluorescence assay | B | 7.89 | pIC50 | 13 | nM | IC50 | Eur J Med Chem (2019) 162: 793-809 [PMID:30522087] |
ChEMBL | Inhibition of human recombinant MAO-B expressed in baculovirus infected BTI-TN-5B1-4 insect cells assessed as decrease in H2O2 production using p-tyramine as substrate incubated for 20 mins by horse-radish peroxidase/amplex red-based fluorescence method | B | 7.89 | pIC50 | 13 | nM | IC50 | Eur J Med Chem (2019) 179: 404-422 [PMID:31265934] |
ChEMBL | Inhibition of recombinant human MAOB expressed in baculovirus infected in BTI cells using kynuramine as substrate after 30 mins by fluorescence based assay | B | 7.94 | pIC50 | 11.6 | nM | IC50 | Bioorg Med Chem (2020) 28: 115374-115374 [PMID:32089390] |
ChEMBL | Inhibition of recombinant human MAO-B using p-tyramine as substrate preincubated for 15 mins followed by substrate addition measured over 15 mins by Amplex red reagent-based fluorimetric method | B | 8 | pIC50 | 10 | nM | IC50 | Eur J Med Chem (2018) 145: 588-593 [PMID:29339253] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using kynuramine as substrate after 30 mins by fluorimetric method | B | 8 | pIC50 | 9.97 | nM | IC50 | Bioorg Med Chem (2017) 25: 1997-2009 [PMID:28237559] |
ChEMBL | Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells using p-tyramine as substrate preincubated for 60 mins followed by substrate addition and measured after 30 mins by horse-radish peroxidase-coupled amplex red reagent-based fluorescence spectrophotometric method | B | 8.08 | pIC50 | 8.4 | nM | IC50 | Eur J Med Chem (2019) 173: 203-212 [PMID:31005056] |
ChEMBL | Inhibition of recombinant human MAO-B using p-tyramine as substrate assessed as decrease in H2O2 production incubated for 15 mins by fluorimetric method | B | 8.1 | pIC50 | 7.87 | nM | IC50 | Bioorg Med Chem (2016) 24: 5929-5940 [PMID:27692996] |
ChEMBL | Inhibition of human MAO-B | B | 8.22 | pIC50 | 6 | nM | IC50 | Bioorg Med Chem Lett (2021) 43: 128051-128051 [PMID:33887441] |
ChEMBL | Inhibition of MAOB | B | 8.36 | pIC50 | 4.4 | nM | IC50 | J Med Chem (2008) 51: 347-372 [PMID:18181565] |
ChEMBL | Inhibition of human MAO-B | B | 8.36 | pIC50 | 4.4 | nM | IC50 | Eur J Med Chem (2020) 205: 112650-112650 [PMID:32920430] |
ChEMBL | Inhibition Assay: MAO-A and MAO-B activity was measured using radioactive substrates. The substrate for MAO-A was 5 HT and for MAO-B was PEA. When measuring the activity of MAO-A, the MAO-B activity was inhibited with deprenyl and when measuring the activity of MAO-B the activity of MAO-A was inhibited with clorgylin. Blank samples were produced using TCP to inhibit both of the enzymes. The metabolites were extracted to toluene and read in a β -counter. The results are expressed in relative activity and normalized to the amount of protein in the tissue. Figs. 1 and 2 show MAO-A/MAO-B activity of compounds 3, 4 and of 0-Methyl-M3O at various concentrations (10 -"5 -10 -"8 ). As presented in Figs. 1 and 2, it can be seen that compounds 3, 4 and 0-Methyl-M3O were all potent inhibitors of MAO-A and MAO-B extracted from rat brain, compound 3 clearly the most potent inhibitor of the three compounds. | B | 8.4 | pIC50 | 4 | nM | IC50 | US-9034303-B2. Neuroprotective and neuro-restorative iron chelators and monoamine oxidase inhibitors and uses thereof (2015) |
Monoamine oxidase B in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2993] [GtoPdb: 2490] [UniProtKB: P19643] | ||||||||
ChEMBL | Inhibition of rat MAOB using benzylamine as substrate pretreated for 1200 secs followed by substrate addition and measured after 3600 secs | B | 4.98 | pIC50 | 10360 | nM | IC50 | Bioorg Med Chem (2018) 26: 4863-4870 [PMID:30143367] |
ChEMBL | Inhibition of rat brain MAO-B in nuclei-free homogenates using [14C]phenylacetaldehyde substrate after 20 mins by liquid scintillation counting | B | 5.52 | pIC50 | 3000 | nM | IC50 | J Med Chem (2015) 58: 1400-1419 [PMID:25627172] |
ChEMBL | Inhibition of MAO-B in Wistar rat liver using 4-trifluoromethyl benzylamine as substrate preincubated for 20 mins followed by substrate addition measured after 90 mins by microplate reader assay | B | 6.64 | pIC50 | 230 | nM | IC50 | Bioorg Med Chem Lett (2020) 30: 126900-126900 [PMID:31882295] |
ChEMBL | Inhibition of MAO-B in rat brain using [14C]-phenylethylamine as substrate preincubated for 60 mins followed by substrate addition and measured after 30 mins by liquid scintillation counting method | B | 8.36 | pIC50 | 4.4 | nM | IC50 | J Med Chem (2019) 62: 8881-8914 [PMID:31082225] |
ChEMBL | In vitro inhibitory concentration against Monoamine oxidase B of rat brain homogenates; value ranges from 0.0035-0.053 | B | 8.36 | pIC50 | 4.4 | nM | IC50 | J Med Chem (2002) 45: 5260-5279 [PMID:12431053] |
ChEMBL | Inhibition of rat brain MAO-B using [14C]-phenylethylamine as substrate preincubated for 60 mins followed by substrate addition and measured after 20 mins by liquid scintillation counting method | B | 8.36 | pIC50 | 4.4 | nM | IC50 | Bioorg Med Chem Lett (2019) 29: 126612-126612 [PMID:31421966] |
ChEMBL | Inhibition of Sprague-Dawley rat liver MAO-B using p-tyramine as substrate preincubated for 15 mins and measured after 45 mins by resorufin-based fluorescence assay | B | 8.37 | pIC50 | 4.31 | nM | IC50 | Eur J Med Chem (2019) 162: 793-809 [PMID:30522087] |
5-HT6 receptor/Serotonin 6 (5-HT6) receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3371] [GtoPdb: 11] [UniProtKB: P50406] | ||||||||
ChEMBL | Displacement of [3H]-LSD from human 5HT6 receptor expressed in HEK293 cells measured after 1 hr by microbeta plate reader method | B | 8.85 | pKi | 1.4 | nM | Ki | Eur J Med Chem (2020) 208: 112765-112765 [PMID:32949963] |
ChEMBL data shown on this page come from version 34:
Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]