ChEMBL ligand: CHEMBL3545369 (Epacadostat, INCB-024360, INCB024360)
Kv11.1/HERG in Human [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809] There should be some charts here, you may need to enable JavaScript!
indoleamine 2,3-dioxygenase 1/Indoleamine 2,3-dioxygenase in Human [ChEMBL: CHEMBL4685] [GtoPdb: 2829] [UniProtKB: P14902] There should be some charts here, you may need to enable JavaScript!
indoleamine 2,3-dioxygenase 1 in Human [GtoPdb: 2829] [UniProtKB: P14902] indoleamine 2,3-dioxygenase 1/Indoleamine 2,3-dioxygenase 1 in Mouse [ChEMBL: CHEMBL1075294] [GtoPdb: 2829] [UniProtKB: P28776] There should be some charts here, you may need to enable JavaScript!
indoleamine 2,3-dioxygenase 2/Indoleamine 2,3-dioxygenase 2 in Human [ChEMBL: CHEMBL3627587] [GtoPdb: 3019] [UniProtKB: Q6ZQW0] indoleamine 2,3-dioxygenase 2/Indoleamine 2,3-dioxygenase 2 in Mouse [ChEMBL: CHEMBL2189159] [GtoPdb: 3019] [UniProtKB: Q8R0V5] There should be some charts here, you may need to enable JavaScript!
tryptophan 2,3-dioxygenase/Tryptophan 2,3-dioxygenase in Human [ChEMBL: CHEMBL2140] [GtoPdb: 2887] [UniProtKB: P48775] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Kv11.1/HERG in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809]
ChEMBL	Inhibition of human ERG	B	4.4	pIC50	>40000	nM	IC50	Eur J Med Chem (2019) 179: 38-55 [PMID:31233921]
ChEMBL	Inhibition of human ERG by fluorescence polarization assay	B	4.44	pIC50	>36000	nM	IC50	J Med Chem (2021) 64: 11302-11329 [PMID:34292726]
indoleamine 2,3-dioxygenase 1/Indoleamine 2,3-dioxygenase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4685] [GtoPdb: 2829] [UniProtKB: P14902]
ChEMBL	Binding affinity to NT650-NHS fluorophore-labeled recombinant human N-terminal His-tagged IDO1 expressed in Escherichia coli measured after 10 mins by microscale thermophoresis assay	B	5.46	pKd	3460	nM	Kd	J Med Chem (2020) 63: 3047-3065 [PMID:32150677]
ChEMBL	Enzyme Assay: Human indoleamine 2,3-dioxygenasae (IDO) with an N-terminal His tag was expressed in E. coli and purified to homogeneity. IDO catalyzes the oxidative cleavage of the pyrrole ring of the indole nucleus of tryptophan to yield N′-formylkynurenine. The assays were performed at room temperature as described in the literature using 95 nM IDO and 2 mM D-Trp in the presence of 20 mM ascorbate, 5 μM methylene blue and 0.2 mg/mL catalase in 50 mM potassium phosphate buffer (pH 6.5). The initial reaction rates were recorded by continuously following the absorbance increase at 321 nm due to the formation of N′-formlylkynurenine (See: Sono, M., et al., 1980, J. Biol. Chem. 255, 1339-1345).	B	6.7	pIC50	<200	nM	IC50	US-9320732-B2. 1,2,5-oxadiazoles as inhibitors of indoleamine 2,3-dioxygenase (2016)
ChEMBL	Inhibition of IDO1 in IFN-gamma/LPS-stimulated human whole blood assessed as decrease in kynurenine levels preincubated for 15 mins followed by IFN-gamma/LPS stimulation for overnight by LC/MS/MS analysis	B	6.9	pIC50	125	nM	IC50	ACS Med Chem Lett (2017) 8: 486-491 [PMID:28523098]
ChEMBL	Inhibition of recombinant human IDO1 expressed in Escherichia coli	B	7.01	pIC50	97	nM	IC50	Bioorg Med Chem Lett (2020) 30: 126901-126901 [PMID:31882299]
ChEMBL	Inhibition of recombinant human IDO1	B	7.03	pIC50	93	nM	IC50	Bioorg Med Chem Lett (2021) 52: 128373-128373 [PMID:34560264]
ChEMBL	Inhibition of IDO1 (unknown origin) assessed as reduction in kynurenine formation using L-tryptophan as substrate by methylene blue reagent based assay	B	7.04	pIC50	91	nM	IC50	J Med Chem (2021) 64: 17950-17968 [PMID:34854662]
ChEMBL	Inhibition of IDO1 (unknown origin)	B	7.05	pIC50	90	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127159-127159 [PMID:32247733]
ChEMBL	Inhibition of recombinant human IDO1 assessed as reduction in kynurenine production using L-tryptophan as substrate incubated for 30 mins by microplate reader analysis	B	7.06	pIC50	87.4	nM	IC50	Eur J Med Chem (2020) 189: 112059-112059 [PMID:31981851]
ChEMBL	Inhibition of recombinant human GST-tagged IDO1 (1 to 404 residues) using D-Tryptophan as substrate	B	7.11	pIC50	77.8	nM	IC50	ACS Med Chem Lett (2018) 9: 312-317 [PMID:29670692]
ChEMBL	Inhibition of IDO1 (unknown origin) assessed as reduction in L-kynurenine formation using L-tryptophan as substrate incubated for 30 mins by microplate spectrophotometry analysis	B	7.12	pIC50	75	nM	IC50	Eur J Med Chem (2020) 207: 112703-112703 [PMID:32871341]
ChEMBL	Inhibition of human N-terminal His-tagged IDO1 expressed in Escherichia coli using D-Trp as substrate	B	7.14	pIC50	73	nM	IC50	ACS Med Chem Lett (2017) 8: 486-491 [PMID:28523098]
ChEMBL	Inhibition of human N-terminal GST-tagged IDO1 expressed in Escherichia coli Rosetta(DE3) pLysS using L-tryptophan as substrate measured after 1 hr by fluorescence assay	B	7.14	pIC50	73	nM	IC50	ACS Med Chem Lett (2020) 11: 179-187 [PMID:32071686]
ChEMBL	Inhibition of human IDO1	B	7.14	pIC50	72	nM	IC50	Bioorg Med Chem (2019) 27: 1087-1098 [PMID:30773421]
ChEMBL	Inhibition of N-terminal His-tagged human recombinant IDO1 expressed in Escherichia coli using D-tryptophan as substrate by methylene blue/ascorbate assay	B	7.14	pIC50	72	nM	IC50	J Med Chem (2015) 58: 9421-9437 [PMID:25970480]
ChEMBL	Inhibition of IDO1 (unknown origin) using D-tryptophan as substrate by spectrophotometric analysis	B	7.14	pIC50	72	nM	IC50	Eur J Med Chem (2018) 143: 656-669 [PMID:29220788]
ChEMBL	Inhibition of IDO1 (unknown origin)	B	7.14	pIC50	72	nM	IC50	J Med Chem (2015) 58: 8762-8782 [PMID:26207924]
ChEMBL	Inhibition of recombinant N-terminal His-tagged human IDO1 expressed in Escherichia coli assessed as reduction in N-formylkynurenine formation using L-tryptophan as substrate	B	7.14	pIC50	72	nM	IC50	Eur J Med Chem (2019) 182: 111629-111629 [PMID:31445231]
ChEMBL	Inhibition of human IDO1	B	7.14	pIC50	71.8	nM	IC50	J Med Chem (2016) 59: 9760-9773 [PMID:27690429]
ChEMBL	Inhibition of N-terminal His-tagged human IDO1 expressed in Escherichia coli assessed as reduction in conversion of D-tryptophan to N-formyl kynurenine by methylene blue dye assay	B	7.14	pIC50	71.8	nM	IC50	J Med Chem (2019) 62: 9161-9174 [PMID:31580660]
ChEMBL	Inhibition of human IDO1 incubated for 60 mins in presence of L-tryptophan by HPLC analysis	B	7.14	pIC50	71.8	nM	IC50	J Med Chem (2016) 59: 9760-9773 [PMID:27690429]
ChEMBL	Inhibition of recombinant human IDO1 expressed in Escherichia coli BL21 cells assessed as reduction in conversion of L-tryptophan to N-formyl kynurenine using L-tryptophan as substrate after 30 mins by methylene blue dye based microplate reader analysis	B	7.15	pIC50	70	nM	IC50	J Med Chem (2019) 62: 9161-9174 [PMID:31580660]
ChEMBL	Inhibition of IDO1 in human HeLa cells using L-tryptophan as substrate after 20 hrs	B	7.15	pIC50	70	nM	IC50	Eur J Med Chem (2019) 162: 455-464 [PMID:30469041]
GtoPdb	-	-	7.17	pIC50	67	nM	IC50	J Med Chem (2009) 52: 7364-7 [PMID:19507862]
ChEMBL	Inhibition of recombinant human N-terminus IDO1 expressed in Escherichia coli assessed as N-formylkynurenine formation using L-tryptophan as substrate measured after 180 mins by UV absorbance based assay	B	7.17	pIC50	67	nM	IC50	Bioorg Med Chem (2016) 24: 6194-6205 [PMID:27769672]
ChEMBL	Inhibition of human IDO1 expressed in Escherichia coli using L-tryptophan as substrate after 1 hr by UV absorption analysis	B	7.17	pIC50	67	nM	IC50	Bioorg Med Chem (2019) 27: 1087-1098 [PMID:30773421]
ChEMBL	Inhibition of IDO1 (unknown origin) assessed as reduction in kynurenine formation using L-tryptophan as substrate incubated for 60 mins by fluorescence based assay	B	7.18	pIC50	66	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127038-127038 [PMID:32088128]
ChEMBL	Inhibition of human IDO1 using D-Trp as substrate	B	7.19	pIC50	64.6	nM	IC50	Eur J Med Chem (2018) 157: 423-436 [PMID:30103191]
ChEMBL	Inhibition of recombinant human IDO1 assessed as reduction in kynurenine formation using L-tryptophan as substrate incubated for 45 mins by microplate reader assay	B	7.24	pIC50	58	nM	IC50	Eur J Med Chem (2020) 188: 111985-111985 [PMID:31881488]
ChEMBL	Inhibition of recombinant human IDO1 expressed in Escherichia coli BL21 (DE3) using tryptophan as substrate	B	7.33	pIC50	47	nM	IC50	Eur J Med Chem (2021) 213: 113163-113163 [PMID:33482599]
ChEMBL	Inhibition of human N-terminal His-tagged IDO1 expressed in Escherichia coli assessed as formation of N'-formyl-kynurenine using D-tryptophan as a substrate by methylene blue based assay	B	7.34	pIC50	46	nM	IC50	Eur J Med Chem (2021) 223: 113631-113631 [PMID:34147748]
ChEMBL	Inhibition of recombinant human IDO1 expressed in Escherichia coli BL21 cells using L-tryptophan as substrate incubated for 30 mins by methylene blue dye based microplate reader analysis	B	7.39	pIC50	40.9	nM	IC50	Eur J Med Chem (2019) 179: 38-55 [PMID:31233921]
ChEMBL	Inhibition of recombinant human IDO1 expressed in Escherichia coli EC538 assessed as reduction in N-formylkynurenine formation using L-tryptophan as substrate incubated for 30 mins by methylene blue reagent based concurrent assay	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem (2021) 39: 116160-116160 [PMID:33901770]
ChEMBL	Inhibition of IDO1 in IFNgamma-stimulated human HeLa cells assessed as reduction in kynurenine formation measured after 48 hrs by spectrophotometry	B	7.45	pIC50	35.76	nM	IC50	J Med Chem (2021) 64: 17950-17968 [PMID:34854662]
ChEMBL	Inhibition of recombinant human IDO1 expressed in Escherichia coli EC538 assessed as reduction in N-formylkynurenine formation using L-tryptophan as substrate incubated for 30 mins by methylene blue reagent based assay	B	7.52	pIC50	30	nM	IC50	Bioorg Med Chem (2021) 39: 116160-116160 [PMID:33901770]
ChEMBL	Inhibition of human IDO1 expressed in Escherichia coli Rosetta using tryptophan as substrate incubated for 1 hr by methylene blue based assay	B	7.59	pIC50	26	nM	IC50	ACS Med Chem Lett (2021) 12: 195-201 [PMID:33603965]
ChEMBL	Inhibition of IDO1 in IFNgamma-stimulated human THP1 cells assessed as reduction in kynurenine productio by spectrophotometric analysis	B	7.64	pIC50	23	nM	IC50	Eur J Med Chem (2018) 143: 656-669 [PMID:29220788]
ChEMBL	Inhibition of IDO1 in human HeLa cells	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127159-127159 [PMID:32247733]
ChEMBL	Inhibition of IDO1 in IFNgamma-stimulated human HeLa cells using tryptophan as substrate measured after 48 hrs	B	7.72	pIC50	19	nM	IC50	Eur J Med Chem (2021) 213: 113163-113163 [PMID:33482599]
ChEMBL	Inhibition of IDO1 in IFN-gamma stimulated human HeLa cells assessed as reduction in kynurenine production using L-tryptophan as substrate incubated for 24 hrs	B	7.8	pIC50	16	nM	IC50	Eur J Med Chem (2019) 184: 111750-111750 [PMID:31610376]
ChEMBL	Inhibition of human IDO1 expressed in HEK293/MSR cells assessed as reduction in kynurenine production incubated fro 2 days	B	7.82	pIC50	15	nM	IC50	Eur J Med Chem (2018) 143: 656-669 [PMID:29220788]
ChEMBL	Inhibition of IDO1 in IFNgamma and LPS-stimulated human dendritic cells co-cultured with human T cells assessed as inhibition of T-cell proliferation in presence of soluble anti-CD3 antibody and human recombinant IL-2 incubated for 2 days	B	7.89	pIC50	13	nM	IC50	Eur J Med Chem (2018) 143: 656-669 [PMID:29220788]
ChEMBL	Inhibition of recombinant human IDO1 expressed in HEK293 cells assessed as N-formylkynurenine formation by measuring kynurenine level incubated for 12 hrs by microplate reader analysis	B	7.9	pIC50	12.6	nM	IC50	Eur J Med Chem (2019) 179: 38-55 [PMID:31233921]
ChEMBL	Inhibition of IDO1 in IFN-gamma stimulated human HeLa cells using L-tryptophan as substrate incubated for 48 hrs by fluorescence assay	B	7.9	pIC50	12.5	nM	IC50	ACS Med Chem Lett (2020) 11: 1548-1554 [PMID:32832022]
ChEMBL	Inhibition of IDO1 in human HeLa cells assessed as production of N-formylkynurenine using tryptophan as substrate in presence of inactivated fetal bovine serum	B	7.9	pIC50	12.5	nM	IC50	Bioorg Med Chem Lett (2021) 49: 128314-128314 [PMID:34391891]
ChEMBL	Inhibition of IDO1 in human HeLa cells using tryptophan as substrate incubated for 24 hrs by RFMS assay	B	7.96	pIC50	11	nM	IC50	J Med Chem (2021) 64: 11302-11329 [PMID:34292726]
ChEMBL	Inhibition of IDO1 in IFN-gamma stimulated human HeLa cells assessed as reduction in kynurenine production incubated for 24 hrs relative to control	B	7.99	pIC50	10.3	nM	IC50	Bioorg Med Chem (2019) 27: 1605-1618 [PMID:30858027]
ChEMBL	Inhibition of human IDO1	B	8	pIC50	10	nM	IC50	J Med Chem (2021) 64: 17950-17968 [PMID:34854662]
ChEMBL	Inhibition of human recombinant IDO1 assessed as reduction in kynurenine production using L-tryptophan incubated for 60 mins by Ehrlich's reagent based assay	B	8	pIC50	10	nM	IC50	Bioorg Med Chem Lett (2018) 28: 651-657 [PMID:29398544]
ChEMBL	Inhibition of IDO1 in human HeLa cells using L-tryptophan as substrate incubated for 48 hrs	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2019) 182: 111629-111629 [PMID:31445231]
ChEMBL	Inhibition of human IDO1 expressed in HEK293T cells assessed as reduction in N-formylkynurenine production incubated for 12 hrs by microplate reader analysis	B	8.02	pIC50	9.63	nM	IC50	Eur J Med Chem (2020) 189: 112059-112059 [PMID:31981851]
ChEMBL	Inhibition of IDO1 in interferon-gamma-induced human SKOV3 cells assessed as N-formylkynurenine formation using L-tryptophan as substrate measured after 24 hrs by fluorescence assay	B	8.1	pIC50	7.9	nM	IC50	ACS Med Chem Lett (2020) 11: 179-187 [PMID:32071686]
ChEMBL	Inhibition of IDO1 in IFNgamma-stimulated human A375 cells assessed as reduction in L-Kyn level measured after 48 hrs by HPLC analysis	B	8.11	pIC50	7.7	nM	IC50	J Med Chem (2020) 63: 3047-3065 [PMID:32150677]
ChEMBL	Inhibition of IDO1 in IFN-gamma-stimulated human HeLa cells assessed as decrease in kynurenine levels after 48 hrs	B	8.13	pIC50	7.4	nM	IC50	ACS Med Chem Lett (2017) 8: 486-491 [PMID:28523098]
ChEMBL	Inhibition of IFN-gamma-stimulated IDO1 activity in human HeLa cells using L-tryptophan as substrate after 48 hrs by microplate reader analysis	B	8.15	pIC50	7.1	nM	IC50	J Med Chem (2015) 58: 9421-9437 [PMID:25970480]
ChEMBL	Inhibition of IFN-gamma stimulated IDO1 in human HeLa cells assessed as reduction in formation of kynurenine incubated for 48 hrs in presence of IFNgamma by microplate reader assay	B	8.15	pIC50	7.1	nM	IC50	J Med Chem (2019) 62: 9161-9174 [PMID:31580660]
ChEMBL	Inhibition of IDO1 in recombinant human IFNgamma and LPS-induced human HeLa assessed as reduction in kynurenine production incubated for 2 days by spectrophotometric analysis	B	8.15	pIC50	7.1	nM	IC50	Eur J Med Chem (2018) 143: 656-669 [PMID:29220788]
ChEMBL	Inhibition of IDO1 in IFN-gamma stimulated human HeLa cells assessed as reduction in kynurenine production incubated for 18 hrs	B	8.15	pIC50	7	nM	IC50	J Med Chem (2019) 62: 9161-9174 [PMID:31580660]
ChEMBL	Inhibition of IDO1 in IFN-gamma/LPS stimulated human THP-1 cells assessed as reduction in kynurenine production after 24 hrs	B	8.25	pIC50	5.6	nM	IC50	Bioorg Med Chem (2019) 27: 1605-1618 [PMID:30858027]
ChEMBL	Inhibition of IDO1 in IFNgamma-stimulated human HeLa cells using L-tryptophan as substrate measured after 48 hrs	B	8.3	pIC50	5	nM	IC50	ACS Med Chem Lett (2021) 12: 195-201 [PMID:33603965]
ChEMBL	Inhibition of recombinant human His-tagged IDO1 (Ala2 to Gly403 residues) expressed in Escherichia coli using tryptophan as substrate preincubated for 10 mins followed by substrate addition and measured after 1 hr by RFMS assay	B	8.46	pIC50	3.5	nM	IC50	J Med Chem (2021) 64: 11302-11329 [PMID:34292726]
ChEMBL	Inhibition of IDO1 in IFNgamma-stimulated human OCI-AML2 cells assessed as reduction in kynurenine production	B	8.47	pIC50	3.4	nM	IC50	Eur J Med Chem (2018) 143: 656-669 [PMID:29220788]
ChEMBL	Inhibition of IFNgamma-stimulated IDO1 in human HeLa cells	B	7.89	pEC50	13	nM	EC50	Bioorg Med Chem Lett (2020) 30: 126901-126901 [PMID:31882299]
ChEMBL	Inhibition of human IDO1 expressed in HEK293 cells assessed as reduction in kynurenine production after 30 mins	B	8.06	pEC50	8.74	nM	EC50	ACS Med Chem Lett (2018) 9: 131-136 [PMID:29456801]
ChEMBL	Inhibition of IDO1 in IFN-gamma stimulated human HeLa cells assessed as reduction in kynurenine formation using L-tryptophan as substrate incubated for 24 hrs by UV-spectrophotometry	B	8.1	pEC50	8	nM	EC50	Bioorg Med Chem Lett (2020) 30: 127038-127038 [PMID:32088128]
indoleamine 2,3-dioxygenase 1 in Human [GtoPdb: 2829] [UniProtKB: P14902]
GtoPdb	-	-	7.17	pIC50	67	nM	IC50	J Med Chem (2009) 52: 7364-7 [PMID:19507862]
indoleamine 2,3-dioxygenase 1/Indoleamine 2,3-dioxygenase 1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075294] [GtoPdb: 2829] [UniProtKB: P28776]
ChEMBL	Inhibition of IDO1 in mouse Panc02 cells assessed as reduction in NFK level incubated for 48 hrs by RFMS analysis	B	7.13	pIC50	74	nM	IC50	J Med Chem (2021) 64: 11302-11329 [PMID:34292726]
ChEMBL	Inhibition of IDO1 in IFNgamma-stimulated mouse Panc02 cells incubated for 48 hrs by RFMS method	B	7.13	pIC50	74	nM	IC50	J Med Chem (2021) 64: 11302-11329 [PMID:34292726]
indoleamine 2,3-dioxygenase 2/Indoleamine 2,3-dioxygenase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3627587] [GtoPdb: 3019] [UniProtKB: Q6ZQW0]
ChEMBL	Inhibition of C-terminal His6-tagged human IDO2 (14 to 420 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in conversion of L-tryptophan to N-formyl kynurenine using L-tryptophan as substrate after 30 mins by methylene blue dye based microplate reader analysis	B	4.99	pIC50	10340	nM	IC50	J Med Chem (2019) 62: 9161-9174 [PMID:31580660]
ChEMBL	Inhibition of N-terminal His-tagged human IDO2 expressed in Escherichia coli expression system	B	6.15	pIC50	710	nM	IC50	Eur J Med Chem (2019) 182: 111629-111629 [PMID:31445231]
indoleamine 2,3-dioxygenase 2/Indoleamine 2,3-dioxygenase 2 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2189159] [GtoPdb: 3019] [UniProtKB: Q8R0V5]
ChEMBL	Inhibition of mouse IDO2 expressed in human T-REx cells using L-tryptophan as substrate after 20 hrs	B	4.72	pIC50	18900	nM	IC50	Eur J Med Chem (2019) 162: 455-464 [PMID:30469041]
tryptophan 2,3-dioxygenase/Tryptophan 2,3-dioxygenase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2140] [GtoPdb: 2887] [UniProtKB: P48775]
ChEMBL	Uncompetitive inhibition of recombinant human TDO expressed in Escherichia coli BL21 (DE3) assessed as assessed as inhibition constant using varying concentration of L-tryptophan as substrate by Dixon plot analysis	B	4.66	pKi	21700	nM	Ki	J Med Chem (2019) 62: 9161-9174 [PMID:31580660]
ChEMBL	Uncompetitive inhibition of recombinant full length C-terminal His-tagged human TDO expressed in Escherichia coli using L-Trp as substrate after 30 mins by Dixon plot analysis	B	4.66	pKi	21700	nM	Ki	Eur J Med Chem (2018) 160: 133-145 [PMID:30321802]
ChEMBL	Inhibition of human TDO expressed in Escherichia coli Rosetta using tryptophan as substrate incubated for 1 hr by methylene blue based assay	B	4	pIC50	>100000	nM	IC50	ACS Med Chem Lett (2021) 12: 195-201 [PMID:33603965]
ChEMBL	Inhibition of recombinant human TDO expressed in Escherichia coli BL21 (DE3) assessed as reduction in conversion of L-tryptophan to N-formyl kynurenine using L-tryptophan as substrate after 30 mins by methylene blue assay	B	4.19	pIC50	64500	nM	IC50	J Med Chem (2019) 62: 9161-9174 [PMID:31580660]
ChEMBL	Inhibition of recombinant full length C-terminal His-tagged human TDO expressed in Escherichia coli using L-Trp as substrate after 30 mins	B	4.19	pIC50	64500	nM	IC50	Eur J Med Chem (2018) 160: 133-145 [PMID:30321802]
ChEMBL	Inhibition of human TDO expressed in human T-REx cells using L-tryptophan as substrate after 20 hrs	B	4.6	pIC50	25200	nM	IC50	Eur J Med Chem (2019) 162: 455-464 [PMID:30469041]
ChEMBL	Inhibition of TDO (unknown origin)	B	5	pIC50	>10000	nM	IC50	ACS Med Chem Lett (2020) 11: 541-549 [PMID:32292562]
ChEMBL	Inhibition of TDO (unknown origin) assessed as reduction in L-kynurenine formation using L-tryptophan as substrate incubated for 30 mins microplate spectrophotometry analysis	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2020) 207: 112703-112703 [PMID:32871341]
ChEMBL	Inhibition of N-terminal His-tagged human TDO expressed in Escherichia coli expression system	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2019) 182: 111629-111629 [PMID:31445231]
ChEMBL	Inhibition of recombinant human TDO2 expressed in Escherichia coli BL21 (DE3) assessed as reduction in N-formylkynurenine formation using L-tryptophan as substrate incubated for 30 mins by methylene blue reagent based concurrent assay	B	5.46	pIC50	3430	nM	IC50	Bioorg Med Chem (2021) 39: 116160-116160 [PMID:33901770]
ChEMBL	Inhibition of recombinant human TDO2 expressed in Escherichia coli BL21 (DE3) assessed as reduction in N-formylkynurenine formation using L-tryptophan as substrate incubated for 30 mins by methylene blue reagent based assay	B	5.67	pIC50	2120	nM	IC50	Bioorg Med Chem (2021) 39: 116160-116160 [PMID:33901770]
ChEMBL	Inhibition of TDO in human U87 MG cells using L-Trp as substrate after 8 hrs	B	6.59	pIC50	260	nM	IC50	Eur J Med Chem (2018) 160: 133-145 [PMID:30321802]
ChEMBL	Inhibition of human TDO transfected in HEK293 cells assessed as reduction in kynurenine production using L-tryptophan as substrate incubated for 12 hrs	B	6.59	pIC50	260	nM	IC50	J Med Chem (2019) 62: 9161-9174 [PMID:31580660]
ChEMBL	Inhibition of TDO (unknown origin) expressed in HEK293 cells using L-Trp as substrate after 8 hrs	B	6.62	pIC50	242	nM	IC50	Eur J Med Chem (2018) 160: 133-145 [PMID:30321802]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]