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ChEMBL ligand: CHEMBL504816 (1-Methyltryptophan) |
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DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
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indoleamine 2,3-dioxygenase 1/Indoleamine 2,3-dioxygenase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4685] [GtoPdb: 2829] [UniProtKB: P14902] | ||||||||
ChEMBL | Inhibition of indoleamine 2,3-dioxygenase in aerobic condition | B | 4.27 | pKi | 53200 | nM | Ki | Bioorg Med Chem (2008) 16: 8661-8669 [PMID:18715787] |
GtoPdb | - | - | 4.37 | pKi | 42300 | nM | Ki | J Med Chem (2013) 56: 8321-31 [PMID:24099220] |
ChEMBL | Competitive inhibition of human recombinant IDO1 using L-tryptophan as substrate | B | 4.37 | pKi | 42300 | nM | Ki | J Med Chem (2013) 56: 8321-8331 [PMID:24099220] |
ChEMBL | Inhibition of IDO | B | 4.43 | pKi | 37000 | nM | Ki | Bioorg Med Chem (2011) 19: 1550-1561 [PMID:21269836] |
ChEMBL | Competitive inhibition of IDO1 (unknown origin) using L-tryptophan as substrate incubated for 30 min assessed as reduction in conversion of N-formyl kynurenine to kynurenine by dixon plot analysis | B | 4.47 | pKi | 34000 | nM | Ki | WO-2017114260-A1. Uses of tryptanthrin and derivative thereof in preparing hldo2 inhibitor (null) |
ChEMBL | Competitive inhibition of human IDO1 in presence of tryptophan | B | 4.47 | pKi | 34000 | nM | Ki | J Med Chem (2013) 56: 8321-8331 [PMID:24099220] |
ChEMBL | Inhibition of IDO1 (unknown origin) | B | 4.47 | pKi | 34000 | nM | Ki | Medchemcomm (2019) 10: 1740-1754 [PMID:32055299] |
ChEMBL | Competitive inhibition of recombinant human IDO1 expressed in Escherichia coli BL21 cells assessed as apparent inhibition constant using varying concentration of L-tryptophan as substrate by Dixon plot analysis | B | 4.47 | pKi | 34000 | nM | Ki | J Med Chem (2019) 62: 9161-9174 [PMID:31580660] |
ChEMBL | Inhibition of IDO1 (unknown origin) | B | 4.52 | pKi | 30000 | nM | Ki | Eur J Med Chem (2017) 138: 199-211 [PMID:28667875] |
ChEMBL | Inhibition of IDO by cell-free assay | B | 4.72 | pKi | 19000 | nM | Ki | J Med Chem (2010) 53: 1172-1189 [PMID:20055453] |
ChEMBL | Competitive inhibition of histidine-tagged human recombinant IDO expressed in bacterial strain BL21 AI using L-Trptophan as substrate measured at 490 nm wavelength after 6 min by colorimetric assay | B | 5.15 | pKi | 7000 | nM | Ki | Eur J Med Chem (2011) 46: 3058-3065 [PMID:21419531] |
ChEMBL | Inhibition of human indoleamine 2,3-dioxygenase expressed in Escherichia coli by spectrophotometric activity assay | B | 5.18 | pKi | 6600 | nM | Ki | J Nat Prod (2006) 69: 1496-1499 [PMID:17067170] |
ChEMBL | Inhibition of recombinant N-terminal His-tagged human IDO (Ala2 to Gly403) overexpressed in Escherichia coli BL21 AI using L-tryptophan as substrate assessed as formation of kynurenine incubated for 5 mins in presence of p-dimethylaminobenzaldehyde | B | 4 | pIC50 | 100000 | nM | IC50 | Eur J Med Chem (2014) 82: 96-105 [PMID:24878638] |
ChEMBL | Inhibition of human IDO activity by spectrophotometry | B | 4 | pIC50 | 100000 | nM | IC50 | Bioorg Med Chem (2011) 19: 1550-1561 [PMID:21269836] |
ChEMBL | Inhibition of purified recombinant human IDO1 using L-tryptophan substrate incubated fro 30 mins | B | 4.01 | pIC50 | 98500 | nM | IC50 | Bioorg Med Chem (2019) 27: 3135-3144 [PMID:31178268] |
ChEMBL | Inhibition of human recombinant IDO expressed in HEK293 cells assessed as blockade of tryptophan degradation by HPLC | B | 4.05 | pIC50 | 90000 | nM | IC50 | J Med Chem (2010) 53: 1172-1189 [PMID:20055453] |
ChEMBL | Inhibition of IFNgamma-induced IDO1 in human HeLa cells using L-tryptophan as substrate after 24 hrs | B | 4.11 | pIC50 | 77290 | nM | IC50 | Eur J Med Chem (2017) 138: 199-211 [PMID:28667875] |
ChEMBL | Inhibition of recombinant human IDO1 expressed in Escherichia coli BL21(DE3) assessed as effect on kynurenine level using L-tryptophan as substrate incubated for 1 hr by Ehrlich's reagent based assay | B | 4.38 | pIC50 | 42000 | nM | IC50 | J Med Chem (2020) 63: 15115-15139 [PMID:33215494] |
ChEMBL | Inhibition of human IDO1 expressed in Escherichia coli BL21 Star assessed as kynurenine formation using L-Trp as substrate preincubated for 90 mins measured after 30 mins by bridge-IT fluorescence assay | B | 4.46 | pIC50 | 35000 | nM | IC50 | Bioorg Med Chem Lett (2016) 26: 4330-4333 [PMID:27469130] |
ChEMBL | Inhibition of recombinant human IDO1 expressed in HEK293 cells assessed as reduction in kynurenine formation after 5 hrs by microplate reader analysis | B | 4.74 | pIC50 | 18400 | nM | IC50 | J Med Chem (2019) 62: 9161-9174 [PMID:31580660] |
ChEMBL | Inhibition of IDO1 in human U87MG cells in presence of L-tryptophan incubated for 24 h | B | 4.74 | pIC50 | 18400 | nM | IC50 | WO-2017114260-A1. Uses of tryptanthrin and derivative thereof in preparing hldo2 inhibitor (null) |
ChEMBL | Inhibition of IDO1 in human HeLa cells | B | 4.74 | pIC50 | 18400 | nM | IC50 | J Med Chem (2020) 63: 15115-15139 [PMID:33215494] |
ChEMBL | Inhibition of human IDO1 expressed in HEK293 cells assessed as kynurenine release after 5 hrs by spectrophotometry | B | 4.74 | pIC50 | 18400 | nM | IC50 | J Med Chem (2013) 56: 8321-8331 [PMID:24099220] |
ChEMBL | Inhibition of His-tagged recombinant human IDO1 expressed in Escherichia coli using tryptophan as substrate by spectrophotometric analysis | B | 5 | pIC50 | 10000 | nM | IC50 | Bioorg Med Chem (2012) 20: 1354-1363 [PMID:22112538] |
ChEMBL | Inhibition of N-terminus 6xHis-tagged human IDO1 expressed in Escherichia coli M15 using L-tryptophan as substrate preincubated for 1 hr measured after 15 mins in presence of bovine liver and methylene blue by HPLC analysis | B | 7.11 | pIC50 | 78 | nM | IC50 | ACS Med Chem Lett (2016) 7: 1167-1172 [PMID:27994758] |
indoleamine 2,3-dioxygenase 1 in Human [GtoPdb: 2829] [UniProtKB: P14902] | ||||||||
GtoPdb | - | - | 4.37 | pKi | 42300 | nM | Ki | J Med Chem (2013) 56: 8321-31 [PMID:24099220] |
indoleamine 2,3-dioxygenase 1/Indoleamine 2,3-dioxygenase 1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075294] [GtoPdb: 2829] [UniProtKB: P28776] | ||||||||
ChEMBL | Inhibition of mouse recombinant IDO expressed in mouse P815B cells assessed as blockade of tryptophan degradation by HPLC | B | 4.4 | pIC50 | 40000 | nM | IC50 | J Med Chem (2010) 53: 1172-1189 [PMID:20055453] |
indoleamine 2,3-dioxygenase 2/Indoleamine 2,3-dioxygenase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3627587] [GtoPdb: 3019] [UniProtKB: Q6ZQW0] | ||||||||
ChEMBL | Inhibition of IDO2 (unknown origin) expressed in human U87MG cells in presence of L-tryptophan incubated for 24 h | B | 4.08 | pIC50 | 82530 | nM | IC50 | WO-2017114260-A1. Uses of tryptanthrin and derivative thereof in preparing hldo2 inhibitor (null) |
ChEMBL | Inhibition of recombinant human C-terminal His6-tagged IDO2 (14-420 residues) expressed in Escherichia coli BL21(DE3) assessed as reduction in L-kynurenine formation using L-tryptophan as substrate after 30 mins in presence of catalase by methylene blue dye based assay | B | 4.08 | pIC50 | 82500 | nM | IC50 | Eur J Med Chem (2016) 123: 171-179 [PMID:27475108] |
ChEMBL | Inhibition of human IDO2 using L-tryptophan as substrate assessed as reduction in conversion of N-formyl kynurenine to kynurenine | B | 4.24 | pIC50 | 56960 | nM | IC50 | WO-2017114260-A1. Uses of tryptanthrin and derivative thereof in preparing hldo2 inhibitor (null) |
ChEMBL | Inhibition of recombinant human IDO2 expressed in human U87MG cells assessed as reduction in kynurenine formation using L-tryptophan as substrate after 6 hrs by spectrophotometry | B | 4.24 | pIC50 | 56900 | nM | IC50 | Eur J Med Chem (2016) 123: 171-179 [PMID:27475108] |
ChEMBL | Inhibition of human IDO2 expressed in Escherichia coli BL21 Star assessed as kynurenine formation using L-Trp as substrate preincubated for 90 mins measured after 30 mins by bridge-IT fluorescence assay | B | 4.46 | pIC50 | 35000 | nM | IC50 | Bioorg Med Chem Lett (2016) 26: 4330-4333 [PMID:27469130] |
ChEMBL data shown on this page come from version 33:
Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]