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Target not currently curated in GtoImmuPdb

Target id: 154

Nomenclature: MRGPRF

Family: Class A Orphans

Gene and Protein Information Click here for help
class A G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 343 11q13.3 MRGPRF MAS related GPR family member F
Mouse 7 343 7 F5 Mrgprf MAS-related GPR, member F
Rat 7 343 1q42 Mrgprf MAS related GPR family member F
Previous and Unofficial Names Click here for help
GPR140 | GPR168 | G-protein coupled receptor 14 | MRGF | G-protein coupled receptor RTA | MAS-related gene F | MAS-related GPR
Database Links Click here for help
Specialist databases
GPCRdb mrgrf_human (Hs), mrgrf_mouse (Mm), mrgrf_rat (Rn)
Other databases
ChEMBL Target
Ensembl Gene
Entrez Gene
Human Protein Atlas
RefSeq Nucleotide
RefSeq Protein
Agonist Comments
MRGPRF has been reported to respond to stimulation by angiotensin metabolites [5].
Tissue Distribution Click here for help
Species:  Mouse
Technique:  RT-PCR
References:  1
Neuronal somata and nerve fibres in enteric plexuses (mysenteric neurons and submucosal neurons), nerve fibres in the tunica muscalaris and in the lamina propria
Species:  Mouse
Technique:  in situ hybridisation
References:  1
Highly expressed throughout large and small intestine, vas deferens, uterus, aorta and cerebellum. Barely detectable in liver, kidney, lung and salivary gland.
Species:  Rat
Technique:  Northern blot
References:  9
Tissue Distribution Comments
MRGPRF expression was not detected by in situ hybridisation in dorsal root ganglia [4].
Gene Expression and Pathophysiology Comments
MRGPRF is downregulated during intestinal schistisomiasis and ileitis [1].
Biologically Significant Variants Click here for help
Type:  Single nucleotide polymorphism
Species:  Human
Amino acid change:  K16R
Global MAF (%):  31
Subpopulation MAF (%):  AFR|AMR|ASN|EUR: 39|26|30|28
Minor allele count:  C=0.307/671
SNP accession: 
Validation:  1000 Genomes, Frequency
Type:  Single nucleotide polymorphism
Species:  Human
Amino acid change:  L63I
Global MAF (%):  31
Subpopulation MAF (%):  AFR|AMR|ASN|EUR: 39|26|30|28
Minor allele count:  T=0.309/675
SNP accession: 
Validation:  1000 Genomes, HapMap, Frequency
General Comments
It was found that Mrgprf expression was upregulated in Mrgpre knockout mice [3].

Of the eight human Mas-related GPCRs (MRGs), four (MRGPRD, MRGPRE, MRGPRF and MRGPRG) have clear orthologues in rodents, whereas the cluster of genes including human MRGPRX1, MRGPRX2, MRGPRX3 and MRGPRX4 is found only in primates and is replaced in rodents with a family of genes (>25 in mice, ~10 in rats) which have no obvious human counterparts [4]. Certain rodent MRGs have been reported to respond to adenine [2] and to RF-amide peptides including neuropeptide FF [6-7] but the relevance of these findings to man is unclear. MRGs are expressed predominantly in small diameter sensory neurons of the dorsal root ganglia, where there is emerging evidence that they may be mediators of histamine-independent itch [8,10].


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1. Avula LR, Buckinx R, Alpaerts K, Costagliola A, Adriaensen D, Van Nassauw L, Timmermans JP. (2011) The effect of inflammation on the expression and distribution of the MAS-related gene receptors MrgE and MrgF in the murine ileum. Histochem Cell Biol, 136 (5): 569-85. [PMID:21912971]

2. Bender E, Buist A, Jurzak M, Langlois X, Baggerman G, Verhasselt P, Ercken M, Guo HQ, Wintmolders C, Van den Wyngaert I et al.. (2002) Characterization of an orphan G protein-coupled receptor localized in the dorsal root ganglia reveals adenine as a signaling molecule. Proc Natl Acad Sci USA, 99 (13): 8573-8. [PMID:12084918]

3. Cox PJ, Pitcher T, Trim SA, Bell CH, Qin W, Kinloch RA. (2008) The effect of deletion of the orphan G - protein coupled receptor (GPCR) gene MrgE on pain-like behaviours in mice. Mol Pain, 4: 2. [PMID:18197975]

4. Dong X, Han S, Zylka MJ, Simon MI, Anderson DJ. (2001) A diverse family of GPCRs expressed in specific subsets of nociceptive sensory neurons. Cell, 106 (5): 619-32. [PMID:11551509]

5. Gembardt F, Grajewski S, Vahl M, Schultheiss HP, Walther T. (2008) Angiotensin metabolites can stimulate receptors of the Mas-related genes family. Mol Cell Biochem, 319 (1-2): 115-23. [PMID:18636314]

6. Han SK, Dong X, Hwang JI, Zylka MJ, Anderson DJ, Simon MI. (2002) Orphan G protein-coupled receptors MrgA1 and MrgC11 are distinctively activated by RF-amide-related peptides through the Galpha q/11 pathway. Proc Natl Acad Sci USA, 99 (23): 14740-5. [PMID:12397184]

7. Lee MG, Dong X, Liu Q, Patel KN, Choi OH, Vonakis B, Undem BJ. (2008) Agonists of the MAS-related gene (Mrgs) orphan receptors as novel mediators of mast cell-sensory nerve interactions. J Immunol, 180 (4): 2251-5. [PMID:18250432]

8. Liu Q, Tang Z, Surdenikova L, Kim S, Patel KN, Kim A, Ru F, Guan Y, Weng HJ, Geng Y, Undem BJ, Kollarik M, Chen ZF, Anderson DJ, Dong X. (2009) Sensory neuron-specific GPCR Mrgprs are itch receptors mediating chloroquine-induced pruritus. Cell, 139 (7): 1353-65. [PMID:20004959]

9. Ross PC, Figler RA, Corjay MH, Barber CM, Adam N, Harcus DR, Lynch KR. (1990) RTA, a candidate G protein-coupled receptor: cloning, sequencing, and tissue distribution. Proc Natl Acad Sci USA, 87 (8): 3052-6. [PMID:2109324]

10. Wilson SR, Gerhold KA, Bifolck-Fisher A, Liu Q, Patel KN, Dong X, Bautista DM. (2011) TRPA1 is required for histamine-independent, Mas-related G protein-coupled receptor-mediated itch. Nat Neurosci, 14 (5): 595-602. [PMID:21460831]


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