ADGRG3 | Adhesion Class GPCRs | IUPHAR/BPS Guide to PHARMACOLOGY

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ADGRG3

Target not currently curated in GtoImmuPdb

Target id: 188

Nomenclature: ADGRG3

Family: Adhesion Class GPCRs

Annotation status:  image of a green circle Annotated and expert reviewed. Please contact us if you can help with updates.  » Email us

Gene and Protein Information
Adhesion G protein-coupled receptor
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human 7 549 16q13 ADGRG3 adhesion G protein-coupled receptor G3 4
Mouse 7 542 8D1 Adgrg3 adhesion G protein-coupled receptor G3
Rat 7 461 19q13 Adgrg3 adhesion G protein-coupled receptor G3
Previous and Unofficial Names
GPR97 (G protein-coupled receptor 97) | Pb99
Database Links
Specialist databases
GPCRDB agrg3_human (Hs), agrg3_mouse (Mm)
Other databases
Ensembl Gene
Entrez Gene
Human Protein Atlas
KEGG Gene
Pharos
RefSeq Nucleotide
RefSeq Protein
UniProtKB
Wikipedia

Download all structure-activity data for this target as a CSV file

Agonists
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Value Parameter Reference
beclometasone dipropionate Hs Full agonist 8.5 pEC50 5
pEC50 8.5 (EC50 3.1x10-9 M) [5]
Antagonist Comments
Treatment with pertussis toxin abolished the agonist-like effect of treatment with beclomethasone dipropionate [5].
Immuno Process Associations
Immuno Process:  Inflammation
GO Annotations:  Associated to 1 GO processes
GO:0043312 neutrophil degranulation TAS
Immuno Process:  B cell (activation)
GO Annotations:  Associated to 1 GO processes
GO:0030183 B cell differentiation ISS
Immuno Process:  Immune system development
GO Annotations:  Associated to 1 GO processes
GO:0030183 B cell differentiation ISS
Immuno Process:  Cellular signalling
GO Annotations:  Associated to 2 GO processes
GO:0030183 B cell differentiation ISS
GO:0043312 neutrophil degranulation TAS
Primary Transduction Mechanisms
Transducer Effector/Response
Gi/Go family
Comments:  ADGRG3 requires chimeric protein Gqo3 to induce inositol phosphate accumulation, indicating it primarily couples to Gαo-protein [5].
References:  5
Tissue Distribution
Unigene expression profile: high frequency in bone marrow, blood, lung.
Species:  Human
Technique: 
References: 
Lung, liver, bone marrow, leukemia cells
Species:  Human
Technique:  EST
References:  1-2,4
Granulocytes
Species:  Human
Technique:  Microarray analysis
References:  7
Unigene expression profile: high frequency in blood, bone marrow, inner ear, thymus, liver, lung, mammary gland.
Species:  Mouse
Technique: 
References: 
Expression Datasets

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Log average relative transcript abundance in mouse tissues measured by qPCR from Regard, J.B., Sato, I.T., and Coughlin, S.R. (2008). Anatomical profiling of G protein-coupled receptor expression. Cell, 135(3): 561-71. [PMID:18984166] [Raw data: website]

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Functional Assays
Beclomethasone dipropionate mediated G protein signaling
Species:  Human
Tissue:  Transfected CHO cell line
Response measured:  Elevated aequorin luminescence signal, [35S]GTPγS binding assay
References:  5
Physiological Consequences of Altering Gene Expression Comments
Adgrg3 knockout mice do not show defects in lymphocyte development [8].
General Comments
ADGRG3 (formerly GPR97) is an orphan receptor belonging to Family VIII Adhesion-GPCRs together with ADGRG1, 2, and 4-7 [3]. The genes of ADGRG1, ADGRG3 and ADGRG5 are syntenically clustered on human chromosome 16 and mouse chromosome 8 suggesting the evolution from an ancestral gene through gene duplication and exon shuffling [7]. Full coding sequence human cDNA is publicly available, IMAGE:6424664 [6], in pOTB7 vector.

References

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1. Bjarnadóttir TK, Fredriksson R, Höglund PJ, Gloriam DE, Lagerström MC, Schiöth HB. (2004) The human and mouse repertoire of the adhesion family of G-protein-coupled receptors. Genomics, 84 (1): 23-33. [PMID:15203201]

2. Bjarnadóttir TK, Geirardsdóttir K, Ingemansson M, Mirza MA, Fredriksson R, Schiöth HB. (2007) Identification of novel splice variants of Adhesion G protein-coupled receptors. Gene, 387 (1-2): 38-48. [PMID:17056209]

3. Fredriksson R, Gloriam DE, Höglund PJ, Lagerström MC, Schiöth HB. (2003) There exist at least 30 human G-protein-coupled receptors with long Ser/Thr-rich N-termini. Biochem. Biophys. Res. Commun., 301 (3): 725-34. [PMID:12565841]

4. Fredriksson R, Lagerström MC, Höglund PJ, Schiöth HB. (2002) Novel human G protein-coupled receptors with long N-terminals containing GPS domains and Ser/Thr-rich regions. FEBS Lett., 531 (3): 407-14. [PMID:12435584]

5. Gupte J, Swaminath G, Danao J, Tian H, Li Y, Wu X. (2012) Signaling property study of adhesion G-protein-coupled receptors. FEBS Lett., 586 (8): 1214-9. [PMID:22575658]

6. Lennon G, Auffray C, Polymeropoulos M, Soares MB. (1996) The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression. Genomics, 33 (1): 151-2. [PMID:8617505]

7. Peng YM, van de Garde MD, Cheng KF, Baars PA, Remmerswaal EB, van Lier RA, Mackay CR, Lin HH, Hamann J. (2011) Specific expression of GPR56 by human cytotoxic lymphocytes. J. Leukoc. Biol., 90 (4): 735-40. [PMID:21724806]

8. Sleckman BP, Khan WN, Xu W, Bassing CH, Malynn BA, Copeland NG, Bardon CG, Breit TM, Davidson L, Oltz EM et al.. (2000) Cloning and functional characterization of the early-lymphocyte-specific Pb99 gene. Mol. Cell. Biol., 20 (12): 4405-10. [PMID:10825203]

Contributors

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