Gene and Protein Information
Species	TM	AA	Chromosomal Location	Gene Symbol	Gene Name	Reference
Human	-	832	21q22.3	RIPK4	receptor interacting serine/threonine kinase 4
Mouse	-	786	16 C4	Ripk4	receptor-interacting serine-threonine kinase 4
Rat	-	786	11q12	Ripk4	receptor-interacting serine-threonine kinase 4

Previous and Unofficial Names

ANKK2 | ankyrin repeat domain 3 | DIK | PKC-delta-interacting protein kinase | PKK | protein kinase C-associated kinase | RIP4

Database Links
Alphafold	P57078 (Hs), Q9ERK0 (Mm)
BRENDA	2.7.11.1
CATH/Gene3D	1.25.40.20
ChEMBL Target	CHEMBL6083 (Hs)
Ensembl Gene	ENSG00000183421 (Hs), ENSMUSG00000005251 (Mm), ENSRNOG00000001618 (Rn)
Entrez Gene	54101 (Hs), 72388 (Mm), 304053 (Rn)
Human Protein Atlas	ENSG00000183421 (Hs)
KEGG Enzyme	2.7.11.1
KEGG Gene	hsa:54101 (Hs), mmu:72388 (Mm), rno:304053 (Rn)
OMIM	605706 (Hs)
Orphanet	ORPHA291802 (Hs)
Pharos	P57078 (Hs)
RefSeq Nucleotide	NM_020639 (Hs), NM_023663 (Mm), NM_001107103 (Rn)
RefSeq Protein	NP_065690 (Hs), NP_076152 (Mm), NP_001100573 (Rn)
UniProtKB	P57078 (Hs), Q9ERK0 (Mm)
Wikipedia	RIPK4 (Hs)

Selected 3D Structures
	Description:  Crystal structure of murine receptor-interacting protein 4 (Ripk4) D143N bound to staurosporine. PDB Id:  5WNL Ligand:  staurosporine Resolution:  2.5Å Species:  Mouse References:  3

Enzyme Reaction
EC Number: 2.7.11.1

DiscoveRx KINOMEscan® screen
A screen of 72 inhibitors against 456 human kinases. Quantitative data were derived using DiscoveRx KINOMEscan® platform. http://www.discoverx.com/services/drug-discovery-development-services/kinase-profiling/kinomescan Reference: 2,5
Key to terms and symbols		Click column headers to sort
Target used in screen: RIPK4
Ligand Sp. Type Action Value Parameter lestaurtinib Hs Inhibitor Inhibition 7.9 pKd TG-100-115 Hs Inhibitor Inhibition 7.0 pKd staurosporine Hs Inhibitor Inhibition 7.0 pKd KW-2449 Hs Inhibitor Inhibition 6.9 pKd AST-487 Hs Inhibitor Inhibition 6.7 pKd tamatinib Hs Inhibitor Inhibition 6.7 pKd PP-242 Hs Inhibitor Inhibition 6.5 pKd tozasertib Hs Inhibitor Inhibition 6.5 pKd vandetanib Hs Inhibitor Inhibition 6.2 pKd foretinib Hs Inhibitor Inhibition 6.1 pKd
Displaying the top 10 most potent ligands  View all ligands in screen »

Clinically-Relevant Mutations and Pathophysiology
Disease:  Popliteal pterygium syndrome 2, lethal type Synonyms: Bartsocas-Papas syndrome [Orphanet: ORPHA1234] Popliteal pterygium syndrome [Disease Ontology: DOID:0060055] Disease Ontology: DOID:0060055 OMIM: 263650 Orphanet: ORPHA1234

General Comments

In humans RIPK4 acts as a protein kinase C (PKC)-δ-interacting protein kinase that is expressed in the epithelium and most non-nervous tissues. It is required for activation of both the NF-κB and JNK-AP-1 pathways in HEK-293T cells [1], and may play a role in Wnt signalling [4]. Huang et al. (2018) have demonstrated in crystallographic studies that a dimeric structure of mouse Ripk4 is required for catalytic activity [3].