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regulator of G-protein signaling 3

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Target not currently curated in GtoImmuPdb

Target id: 2810

Nomenclature: regulator of G-protein signaling 3

Abbreviated Name: RGS3

Family: R4 family

Gene and Protein Information Click here for help
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human - 1198 9q32 RGS3 regulator of G protein signaling 3 2
Mouse - 966 4 33.19 cM Rgs3 regulator of G-protein signaling 3
Rat - 967 5q24 Rgs3 regulator of G-protein signaling 3
Previous and Unofficial Names Click here for help
Database Links Click here for help
Ensembl Gene
Entrez Gene
Human Protein Atlas
RefSeq Nucleotide
RefSeq Protein
Associated Proteins Click here for help
G Proteins
Name References
Interacting Proteins
Name Effect References
14-3-3 protein Inhibits GAP activity of RGS3. 31,38
GluA2 Regulates glutamatergic synapses. 25
EPH receptor B2 Inhibits heterotremeric G protein signal. 22,45
Phospholipid scramblase 1 (PLSCR1) 1,39
Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 9
Polyubiquitin-C 18
Amyloid beta (A4) precursor protein 32
Arrestin, beta 1 49
Interferon, alpha-inducible protein 46
Tissue Distribution Click here for help
Brain, heart, kindey, liver and pancreas, digestive tract, urinary bladder, male reproductive system, breast and female reproductive system, skin, blood and immune system, respiratory system.
Species:  Human
Technique:  Immunohistochemistry.
References:  6,14,20,26
Heart, brain, kindey, blood and immune system.
Species:  Mouse
Technique:  Immunohistochemistry.
References:  14,21,30,37,48
Heart, spleen, brain, testis, intestine, uterus, ovary, lung.
Species:  Rat
Technique:  Western blot and immunohistochemistry.
References:  16,19,53
Functional Assays Click here for help
Positive regulation of GTPase activity.
Species:  Human
Tissue:  HEK293 cells and mouse NIH 3T3 fibroblasts.
Response measured:  Increase in GTPase activation.
References:  10
Translocation to the membrane.
Species:  Human
Tissue:  HEK293 cells and mouse NIH 3T3 fibroblasts.
Response measured:  Regulation of G-protein coupled receptor protein signaling pathway.
References:  10
Inactivation of MAPK activity.
Species:  Human
Tissue:  Mammalian cells.
Response measured:  Inactivation of MAPK activity.
References:  8
Impairs signal transduction through the muscarinic M1 receptor and beta-adrenergic receptor.
Species:  Human
Tissue:  HEK 293T cells.
Response measured:  Inhibition of IP3 production.
References:  40
Attenuation of Gqαα protein activation of phospholipase C.
Species:  Unknown
Tissue:  COS1 cels.
Response measured:  Inhibition of IP3 production.
References:  27
RGS3T (a truncated variant comprising amino acids 314–519) is localized to the nucleus and induces apoptosis.
Species:  Human
Tissue:  Chinese hamster ovary (CHO) cells.
Response measured:  Apoptosis assay.
References:  9
Physiological Functions Click here for help
Inhibition of MEK-ERK1/2 signaling.
Species:  Mouse
Tissue:  Heart.
References:  21
Modulating the LH secretory responsiveness of the pituitary gonadotrope to GnRH.
Species:  Human
Tissue:  Gonadotropic alpha T3-1 cells.
References:  27
Shaping the magnitude and kinetics of physiological events.
Species:  Human
Tissue:  HEK293 cells.
References:  24
Promotes epithelial mesenchymal transition.
Species:  Human
Tissue:  HEK 293 cells.
References:  43
Cortical development: KIF20A is essential for maintaining progenitor cell state and acts by facilitating the localization of RGS3 to the ICB/midbody of dividing NPCs where KIF20A, RGS3, and Gα subunit may work together to control the outcome of cell division in mice.
Species:  Mouse
References:  13
RGS3 might be an essential factor in follicular growth and have a potential inhibitory effect on follicle number; RGS3 can regulate follicle development and growth via activation of the NFκB signaling pathway.
Species:  Chicken
References:  42
RGS3 may provide protection against pathological changes of adventitial fibroblasts and the development of atherosclerosis by inhibiting TGF-β1/Smad signaling.
Species:  None
References:  50
Potent inhibition of angiotensin AT1 receptor signaling
Species:  None
References:  44
Involved in shortening neutrophil lifespan in response to the endogenous ribosomal protein S19 ploymer (which is a C5a mimic).
Species:  Human
Tissue:  HL-60 neutrophils
References:  29
MicroRNA‑126‑3p inhibits the proliferation, migration, invasion, and angiogenesis of triple‑negative breast cancer cells by targeting RGS3
Species:  Human
References:  15
The RGS3-KIF20A axis regulates the balance between proliferation and differentiation in the mammalian cortex via a mechanism that is independent of spindle/cleavage plane orientation.
Species:  None
References:  34
Physiological Consequences of Altering Gene Expression Click here for help
Overexpression of RGS3 protects against cardiac hypertrophy.
Species:  Mouse
Tissue:  Heart.
Technique:  Gene over-expression.
References:  21
Transformation of HL-60 cells to overexpress RGS3 promotes apoptosis in association with the downregulation of the extracellular signal-regulated kinase signal, and vice versa in the RGS3 knocked-down cells.
Species:  Mouse
Tissue:  HL-60 cells, fate of Q137N-HL-60 cells.
Technique:  RNA interference (RNAi).
References:  28
RGS3 deficiency causes early cell cycle exit and precocious differentiation in neural progenitor cells of the developing cerebral cortex, leading to a loss of cortical neural progenitor cells.
Species:  Mouse
Tissue:  Embryonic brain cells.
Technique:  Gene knock-out.
References:  35
RGS3 is significantly upregulated in gastric cancer tumours (regulated by microRNA-126), and is associated with a poor prognosis.
Species:  Human
References:  47
RGS3, a potential inhibitor of the MEK-ERK1/2 signaling axis, was found to be downregulated by ectopic noncoding RNA HOXD-AS1 overexpression, leading to a reduced apoptotic effect.
Species:  Human
Tissue:  Human hepatocellular carcinoma cells
References:  23
RGS3 appears to be upregulated by inflammatory processes that are associated with neuropathic pain,
Species:  Rat
Technique:  Rat model of neuropathic pain
References:  7
RGS3 inhibition by miR-25 which is upregulated in NSCLC, is associated with inhibition of apoptosis.
Species:  Human
Tissue:  Human lung cancer cells
References:  5
Expression of RGS3 was inhibited by adenosine agonists in both human astrocytoma cells and rat astrocytes.
Species:  Human
Tissue:  Astrocytes and astrocytoma cells
References:  11
Physiological Consequences of Altering Gene Expression Comments
RGS3 is upregulated in p53-mutated breast tumors, and may be a useful predictor of response to docetaxel [33].
RGS3 deficient C. elegans fail to respond to intense sensory stimuli [12].
Xenobiotics Influencing Gene Expression Click here for help
Rgs3 gene expression is repressed by high-fat feeding.
Species:  Mouse
Tissue:  Hepatic cells.
Technique:  Misroarray.
References:  36
Gene Expression and Pathophysiology Comments
Experiments in rats suggests that modulation of Rgs3 gene expression (and/or other genes involved in the control of G-protein signalling) may play a role the pathology of hypertension-associated bladder dysfunction [52].
Biologically Significant Variants Click here for help
Type:  mRNA transcript variant.
Species:  Human
Description:  This RGS3 variant enhances canonical Wnt signaling and promotes epithelial mesenchymal transition via inhibition of G-protein-mediated MAP kinase activation
Amino acids:  93
Nucleotide accession: 
Protein accession: 
References:  8,43
Type:  mRNA transcript variant.
Species:  Human
Description:  This RGS3 variant enhances canonical Wnt signaling and promotes epithelial mesenchymal transition.
Amino acids:  311
Nucleotide accession: 
Protein accession: 
References:  3-4,17
Type:  mRNA transcript variant.
Species:  Human
Description:  This RGS3 variant leads to inhibition of G-protein-mediated MAP kinase activation.
Amino acids:  519
Nucleotide accession: 
Protein accession: 
References:  2,4,8
Type:  mRNA transcript variant.
Species:  Human
Description:  This variant localizes RGS3 to the nucleus and induces apoptosis.
Amino acids:  519
Nucleotide accession: 
Protein accession: 
References:  2
Type:  mRNA transcript variant.
Species:  Human
Description:  This RGS3 variant inhibits G-protein-mediated signal transduction.
Amino acids:  1198
Nucleotide accession: 
Protein accession: 
References:  4,41
Type:  mRNA transcript variant.
Species:  Human
Amino acids:  917
Nucleotide accession: 
Protein accession: 
References:  4,17,43
Biologically Significant Variant Comments
A multi-trait approach identified rs144636307 as a rare mutation in RGS3 that is associated with abnormalities of cardiac structure and function [51].


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