SARS-CoV-2 Replicase polyprotein 1a | SARS-CoV-2 proteins | IUPHAR/BPS Guide to PHARMACOLOGY

Top ▲

SARS-CoV-2 Replicase polyprotein 1a

Target not currently curated in GtoImmuPdb

Target id: 3124

Nomenclature: SARS-CoV-2 Replicase polyprotein 1a

Family: SARS-CoV-2 proteins

Annotation status:  image of a grey circle Awaiting annotation/under development. Please contact us if you can help with annotation.  » Email us

Database Links
UniProtKB
Selected 3D Structures
Image of receptor 3D structure from RCSB PDB
Description:  Crystal Structure of ADP ribose phosphatase of NSP3 from SARS CoV-2 in the complex with ADP ribose
PDB Id:  6W02
Resolution:  1.5Å
Species:  SARS-CoV-2
References: 
Image of receptor 3D structure from RCSB PDB
Description:  The crystal structure of Nsp9 RNA binding protein of SARS CoV-2
PDB Id:  6W4B
Resolution:  2.95Å
Species:  SARS-CoV-2
References: 
Image of receptor 3D structure from RCSB PDB
Description:  Crystal Structure of ADP ribose phosphatase of NSP3 from SARS-CoV-2 in the apo form.
PDB Id:  6WEN
Resolution:  1.35Å
Species:  SARS-CoV-2
References: 
Image of receptor 3D structure from RCSB PDB
Description:  Peptide-bound SARS-CoV-2 Nsp9 RNA-replicase.
PDB Id:  6W9Q
Resolution:  2.05Å
Species:  SARS-CoV-2
References: 
General Comments
The pp1a polyprotein is proteolytically cleaved to form 11 smaller proteins. Most of what is predicted about the functions of these proteins is implied by similarities to orthologues from other coronaviruses [8].

Non-structural protein 1: nsp1 binds to host 40S ribosomes and shuts down host translation [9].

Non-structural protein 2: Interacts with host prohibitin (PHB) and prohibitin 2 (PHB2) [6] which may compromise mitochondrial respiration and intracellular signalling.

Non-structural protein 3 (papain-like protease, PL-pro): Protease activity cleaves motifs at the N-terminus of the replicase polyprotein. PL-pro also removes polyubiquitin chains from cellular substrates, and takes part in the assembly of virally-induced cytoplasmic double-membrane vesicles with nsp4. Blocks host IRF3 activation and NF-κB signalling to down-modulate the host anti-viral immune response. Has potential phosphatase activity [11]. An X-ray structure of SARS-CoV PL-pro in complex with an inhibitor compound has been submitted to the RCSB Protein Data Bank (4OW0) [5]. An HCV protease inhibitor, asunaprevir, has been shown to inhibit PL-pro from SARS-CoV, MERS-CoV and SARS-CoV-2 [3].

Non-structural protein 4: Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles with PL-pro [2].

Non-structural protein 5 is more commonly known as 3CL-protease or main protease (Mpro): cleaves motifs in the C-terminus of the replicase polyproteins and is crucial for viral replication. Molecular target for the development of inhibitors with anti-coronavirus activity.

Non-structural protein 6: Coronavirus nsp6 proteins limit autophagosome expansion [7]. This mechanism may favour coronavirus infection by damaging autophagosome-mediated delivery of viral components to lysosomes for degradation.

Non-structural protein 7: Forms a hexadecameric complex with nsp8 (8 subunits of each) that appears to be involved in viral replication (primase activity) [12].

Non-structural protein 8 Forms a hexadecameric complex with nsp7 (8 subunits of each) that appears to be involved in viral replication (primase activity) [12]

Non-structural protein 9 May be a ssRNA-binding protein that contributes to viral replication [10].

Non-structural protein 10 Stimulates nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities, so is essential for viral mRNAs cap methylation [4].

Non-structural protein 11 behaves as a RNA-directed RNA polymerase that copies the viral RNA template [1].

References

Show »

1. Ahn DG, Choi JK, Taylor DR, Oh JW. (2012) Biochemical characterization of a recombinant SARS coronavirus nsp12 RNA-dependent RNA polymerase capable of copying viral RNA templates. Arch. Virol., 157 (11): 2095-104. [PMID:22791111]

2. Angelini MM, Akhlaghpour M, Neuman BW, Buchmeier MJ. (2013) Severe acute respiratory syndrome coronavirus nonstructural proteins 3, 4, and 6 induce double-membrane vesicles. mBio, 4 (4). DOI: 10.1128/mBio.00524-13 [PMID:23943763]

3. Anson BJ, Chapman ME, Lendy EK, Pshenychnyi S, D’Aquila RT, Satchell KJF, Mesecar AD. (2020) Broad-spectrum inhibition of coronavirus main and papain-like proteases by HCV drugs. Nature Research, PrePrint, Under Review. DOI: 10.21203/rs.3.rs-26344/v1

4. Bouvet M, Imbert I, Subissi L, Gluais L, Canard B, Decroly E. (2012) RNA 3'-end mismatch excision by the severe acute respiratory syndrome coronavirus nonstructural protein nsp10/nsp14 exoribonuclease complex. Proc. Natl. Acad. Sci. U.S.A., 109 (24): 9372-7. [PMID:22635272]

5. Báez-Santos YM, Barraza SJ, Wilson MW, Agius MP, Mielech AM, Davis NM, Baker SC, Larsen SD, Mesecar AD. (2014) X-ray structural and biological evaluation of a series of potent and highly selective inhibitors of human coronavirus papain-like proteases. J. Med. Chem., 57 (6): 2393-412. [PMID:24568342]

6. Cornillez-Ty CT, Liao L, Yates 3rd JR, Kuhn P, Buchmeier MJ. (2009) Severe acute respiratory syndrome coronavirus nonstructural protein 2 interacts with a host protein complex involved in mitochondrial biogenesis and intracellular signaling. J. Virol., 83 (19): 10314-8. [PMID:19640993]

7. Cottam EM, Whelband MC, Wileman T. (2014) Coronavirus NSP6 restricts autophagosome expansion. Autophagy, 10 (8): 1426-41. [PMID:24991833]

8. Fung TS, Liu DX. (2019) Human Coronavirus: Host-Pathogen Interaction. Annu. Rev. Microbiol., 73: 529-557. [PMID:31226023]

9. Lokugamage KG, Narayanan K, Huang C, Makino S. (2012) Severe acute respiratory syndrome coronavirus protein nsp1 is a novel eukaryotic translation inhibitor that represses multiple steps of translation initiation. J. Virol., 86 (24): 13598-608. [PMID:23035226]

10. Miknis ZJ, Donaldson EF, Umland TC, Rimmer RA, Baric RS, Schultz LW. (2009) Severe acute respiratory syndrome coronavirus nsp9 dimerization is essential for efficient viral growth. J. Virol., 83 (7): 3007-18. [PMID:19153232]

11. Saikatendu KS, Joseph JS, Subramanian V, Clayton T, Griffith M, Moy K, Velasquez J, Neuman BW, Buchmeier MJ, Stevens RC et al.. (2005) Structural basis of severe acute respiratory syndrome coronavirus ADP-ribose-1''-phosphate dephosphorylation by a conserved domain of nsP3. Structure, 13 (11): 1665-75. [PMID:16271890]

12. te Velthuis AJ, van den Worm SH, Snijder EJ. (2012) The SARS-coronavirus nsp7+nsp8 complex is a unique multimeric RNA polymerase capable of both de novo initiation and primer extension. Nucleic Acids Res., 40 (4): 1737-47. [PMID:22039154]

How to cite this page

Select citation format: