In intestinal mucosa, EP3 receptors are expressed on epithelia at the apex of crypts. |
Species: |
Human |
Technique: |
in situ hybridization, immunohistochemistry. |
References: |
124 |
|
|
EP3 receptor is primarily expressed in the cortical and outer medullary collecting duct, as well as in the medullary thick ascending limb . |
Species: |
Human |
Technique: |
in situ hybridization. |
References: |
17-18 |
|
|
EP3 receptor labeling was primarily observed in the corneal endothelium and keratocytes, trabecular cells, ciliary epithelium, and conjunctival and iridal stroma cells, and EP(3) was found, in addition, in retinal Müller cells. |
Species: |
Human |
Technique: |
RT-PCR, immunohistochemistry. |
References: |
109 |
|
|
In pregnant myometrium EP3 receptor was only expressed by stromal and endothelial cells. |
Species: |
Human |
Technique: |
Western blot, immunohistochemistry. |
References: |
69 |
|
|
In myometrium EP3 receptor expression is increased in placenta, chorion and amnion with labour. |
Species: |
Human |
Technique: |
in situ hybridization., immunohistochemistry. |
References: |
140 |
|
|
Kidney and uterus. |
Species: |
Human |
Technique: |
RT-PCR. |
References: |
5 |
|
|
Thalamus: anterior, ventromedial, laterodorsal, paraventricular and central medial nuclei. |
Species: |
Human |
Technique: |
in situ hybridisation. |
References: |
122 |
|
|
Mouth: gingival fibroblasts. |
Species: |
Human |
Technique: |
RT-PCR. |
References: |
92 |
|
|
Kidney: glomeruli, Tamm-Horsfall negative late distal convoluted tubules, connecting segments, cortical and medullary collecting ducts, media and endothelial cells of arteries and arterioles. |
Species: |
Human |
Technique: |
Immunohistochemistry. |
References: |
81 |
|
|
There were no differences between non-pregnant and non-labouring pregnant subjects in mRNA expression of EP3-II,-III and -VI. However, there is decreased mRNA expression of lower-uterine segment EP3 receptor isoforms II and VI during labour. |
Species: |
Human |
Technique: |
RT-PCR. |
References: |
68 |
|
|
Kidney: cortical and outer medullary collecting duct, medullary thick ascending limb. |
Species: |
Human |
Technique: |
in situ hybridization. |
References: |
18 |
|
|
Eye: corneal endothelium and keratocytes, trabecular cells, ciliary epithelium, conjunctival and iridal stroma cells, retinal Muller cells. |
Species: |
Human |
Technique: |
Immunohistochemistry. |
References: |
109 |
|
|
EP3-Ib, EP3-II, EP3-III, EP3-IV and EP3-e mRNA in vascular smooth muscle and gastric fundic mucosa cells. |
Species: |
Human |
Technique: |
RT-PCR, Southern blot, immunohistochemistry. |
References: |
59 |
|
|
Myometrium. |
Species: |
Human |
Technique: |
Northern blotting. |
References: |
74 |
|
|
EP3 receptor expression in the mouse preoptic region by in situ hybridization and isolated the corresponding area by laser capture microdissection. Real-time RT-PCR analysis of microdissected tissue revealed a predominant expression of the EP3-α isoform |
Species: |
Mouse |
Technique: |
in situ hybridization. |
References: |
142 |
|
|
Ammon's horn, the preoptic nuclei, lateral hypothalamic area, dorsomedial hypothalamic nucleus, lateral mammillary nucleus, entopeduncular nucleus, substantia nigra pars compacta, locus coeruleus and raphe nuclei > mitral cell layer of the main olfactory bulb, layer V of the entorhinal and parasubicular cortices, layers V and VI of the cerebral neocortex, nuclei of the diagonal band, magnocellular preoptic nucleus, globus pallidus and lateral parabrachial nucleus. |
Species: |
Mouse |
Technique: |
in situ hybridisation. |
References: |
122 |
|
|
Kidney: tubules in the outer medulla and in the distal tubules in the cortex. |
Species: |
Mouse |
Technique: |
in situ hybridization. |
References: |
120 |
|
|
Gastrointestinal tract: neurons of the myenteric ganglia throughout the tract > fundic gland epithelial cells, except for surface mucous cells in the stomach. |
Species: |
Mouse |
Technique: |
in situ hybridization. |
References: |
82 |
|
|
EP3 receptor mRNA is expressed in the tubular epithelium of the outer medulla, especially in the thick ascending limb and cortical collecting ducts. |
Species: |
Mouse |
Technique: |
in situ hybridization. |
References: |
120-121 |
|
|
Main cell species expressing EP3 in the skin is keratinocytes. |
Species: |
Mouse |
Technique: |
Immunohistochemistry, histology in receptor knockout mice |
References: |
38 |
|
|
Intense signals for EP3 transcripts were detected in neurons of the myenteric ganglia throughout the tract. Moderate EP3 mRNA expression was also observed in fundic gland epithelial cells. |
Species: |
Mouse |
Technique: |
in situ hybridization. |
References: |
82 |
|
|
Aorta. |
Species: |
Mouse |
Technique: |
RNase protection assay. |
References: |
155 |
|
|
In tumor obtained after Sarcoma-180 cells implantation, EP3 mRNA are localized in both sarcoma and stromal cells but not in endothelial cells. |
Species: |
Mouse |
Technique: |
in situ hybridization. |
References: |
4 |
|
|
Kidney: outer medulla > cortex > papilla. |
Species: |
Rat |
Technique: |
RNase protection assay. |
References: |
45 |
|
|
Gastrointestinal tract: small intestine and colon (muscle layers of the deep intestinal wall), stomach (gastric mucosal layer containing epithelial cells). |
Species: |
Rat |
Technique: |
Northern blotting. |
References: |
23 |
|
|
EP3-like immunoreactivity was observed in the median preoptic nucleus, medial preoptic area, parastrial nucleus, compact part of the dorsomedial hypothalamic nucleus, and dorsal part of the premammillary nucleus. |
Species: |
Rat |
Technique: |
Western blot, immunohistochemistry. |
References: |
84,86 |
|
|
Tissue Distribution Comments |
Prostanoid receptor antibodies, are not very selective for immunochemistry experiments, results with other experimental approaches in parallel are necessary. |