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TRPC2

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Target not currently curated in GtoImmuPdb

Target id: 487

Nomenclature: TRPC2

Family: Transient Receptor Potential channels (TRP)

Contents:

Gene and Protein Information Click here for help
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human - 0 - 11p15.4-15.3 TRPC2 transient receptor potential cation channel subfamily C member 2 (pseudogene) 13
Mouse 6 1 1172 7 54.71 cM Trpc2 transient receptor potential cation channel, subfamily C, member 2 3,12
Rat 7 1 1170 1q32 Trpc2 transient receptor potential cation channel subfamily C member 2 4
Gene and Protein Information Comments
TRPC2 is a possible pseudogene in humans.
Previous and Unofficial Names Click here for help
short transient receptor potential channel 2 | trp2 | TRPC2a | TRPC2b | transient receptor potential cation channel, subfamily C, member 2, pseudogene | transient receptor potential cation channel | transient receptor potential cation channel subfamily C member 2, pseudogene
Database Links Click here for help
Alphafold Q9R244 (Mm), Q9R283 (Rn)
Ensembl Gene ENSG00000182048 (Hs), ENSMUSG00000100254 (Mm), ENSRNOG00000020188 (Rn)
Entrez Gene 7221 (Hs), 22064 (Mm), 64573 (Rn)
Human Protein Atlas ENSG00000182048 (Hs)
KEGG Gene hsa:7221 (Hs), mmu:22064 (Mm), rno:64573 (Rn)
RefSeq Nucleotide NM_011644 (Mm), NM_001109897 (Mm)
RefSeq Protein NP_001103367 (Mm), NP_035774 (Mm)
UniProtKB Q9R244 (Mm), Q9R283 (Rn)
Wikipedia TRPC2 (Hs)
Associated Proteins Click here for help
Heteromeric Pore-forming Subunits
Name References
TRPC6 2
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
junctate 8
Enkurin 10
calmodulin 13
IP3R3 1
Functional Characteristics Click here for help
γ = 42 pS linear single channel conductance in 150 mM symmetrical Na+ in vomeronasal sensory neurons. PCa/PNa = 2.7; permeant to Na+, Cs+, Ca2+, but not NMDG [6,14]
Ion Selectivity and Conductance Click here for help
Species:  Mouse
Rank order:  Ca2+ [42.0 pS] = Na+
References:  5
Ion Selectivity and Conductance Comments
Selectivity rank order not determined, cation nonselective and Ca2+ permeant. Quoted conductance is in symmetrical 140mM Na+
Chemical activators (Human)
Diacylglycerol (SAG, OAG, DOG): strongly inhibited by Ca2+/CaM once activated by DAG  [7]
Physical activators (Human)
DAG kinase; regulates DAG concentration in vomeronasal sensory neurons

Download all structure-activity data for this target as a CSV file go icon to follow link

Activators
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Value Parameter Concentration range (M) Holding voltage (mV) Reference
DOG Small molecule or natural product Click here for species-specific activity table Mm Agonist - - 1x10-4 -80.0 5
⤷ Conc range: 1x10-4 M [5]
Holding voltage: -80.0 mV
OAG Small molecule or natural product Click here for species-specific activity table Mm Agonist - - 1x10-4 -80.0 5
⤷ Conc range: 1x10-4 M [5]
Holding voltage: -80.0 mV
SAG Small molecule or natural product Mm Agonist - - 1x10-4 -80.0 5
⤷ Conc range: 1x10-4 M [5]
Holding voltage: -80.0 mV
Intracellular Ca2+ Ligand is endogenous in the given species N/A - - - - -
⤷
View species-specific activator tables
Channel Blockers
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Value Parameter Concentration range (M) Holding voltage (mV) Reference
2-APB Small molecule or natural product Click here for species-specific activity table Ligand has a PDB structure Mm Antagonist - - 5x10-5 -70.0 – 80.0 5
⤷ Conc range: 5x10-5 M [5]
Holding voltage: -70.0 – 80.0 mV
U73122 Small molecule or natural product Mm Antagonist - - 1x10-5 -
⤷ Conc range: 1x10-5 M may be indirect
Tissue Distribution Click here for help
Testes, sperm, heart, kidney, brain.
Species:  Mouse
Technique:  Northern Blot
References:  12
Vomeronasal organ (TRPC2-β splice variant).
Species:  Mouse
Technique:  Northern Blot, β-galactosidase staining.
References:  3,5
Vomeronasal organ (sensory villi).
Species:  Rat
Technique:  Northern Blot, Immunohistochemistry
References:  4
Tissue Distribution Comments
Most reports show TRPC2 in the vomeronasal organ only.
Functional Assays Click here for help
Patch clamp, whole-cell and single channel measurements of a DAG-activated channel present in wild-type, but not trpc2-/- mice.
Species:  Mouse
Tissue:  VNO dendrites.
Response measured:  Current.
References:  5
Behaviour: loss of sex discrimination, lack of male-male aggression.
Species:  Mouse
Tissue:  In vivo (vomeronasal organ).
Response measured:  Aggression and mating behaviour.
References:  9
Physiological Functions Click here for help
Sensory transduction in the vomeronasal organ.
Species:  Mouse
Tissue:  Vomeronasal organ.
References:  4-5,9
Putative role in acrosome reaction in sperm.
Species:  Mouse
Tissue:  Spermatozoa.
References:  3
Putative role in erythropoetin-mediated Ca2+ influx in erythroblasts.
Species:  Mouse
Tissue:  Blood.
References:  11
Physiological Consequences of Altering Gene Expression Click here for help
The trpc2 double knockout mouse has normal fertility, but lacks pheromone sensation and mates indiscriminately with males and females.
Species:  Mouse
Tissue: 
Technique:  Altered gene expression in ES cells
References:  5,9
Phenotypes, Alleles and Disease Models Click here for help Mouse data from MGI

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Allele Composition & genetic background Accession Phenotype Id Phenotype Reference
Trpc2tm1Dlc Trpc2tm1Dlc/Trpc2tm1Dlc
involves: 129S1/Sv * C57BL/6		 MGI:109527  MP:0009945 abnormal accessory olfactory bulb morphology PMID: 11823606 
Trpc2tm1Rax Trpc2tm1Rax/Trpc2tm1Rax
involves: 129S/SvEv * C57BL/6J		 MGI:109527  MP:0001375 abnormal mating preference PMID: 11972034 
Trpc2tm1Dlc Trpc2tm1Dlc/Trpc2tm1Dlc
involves: 129S1/Sv * C57BL/6		 MGI:109527  MP:0001375 abnormal mating preference PMID: 11823606 
Trpc2tm1Rax Trpc2tm1Rax/Trpc2tm1Rax
involves: 129S/SvEv * C57BL/6J		 MGI:109527  MP:0002272 abnormal nervous system electrophysiology PMID: 11972034 
Trpc2tm1Dlc Trpc2tm1Dlc/Trpc2tm1Dlc
involves: 129S1/Sv * C57BL/6		 MGI:109527  MP:0002272 abnormal nervous system electrophysiology PMID: 11823606 
Trpc2tm1Dlc Trpc2tm1Dlc/Trpc2tm1Dlc
involves: 129S1/Sv * C57BL/6		 MGI:109527  MP:0000967 abnormal sensory neuron projections PMID: 11823606 
Trpc2tm1Rax Trpc2tm1Rax/Trpc2tm1Rax
involves: 129S/SvEv		 MGI:109527  MP:0002566 abnormal sexual interaction PMID: 20596023 
Trpc2tm1Dlc Trpc2tm1Dlc/Trpc2tm1Dlc
involves: 129S1/Sv * C57BL/6		 MGI:109527  MP:0002243 abnormal vomeronasal organ morphology PMID: 11823606 
Trpc2tm1Rax Trpc2tm1Rax/Trpc2tm1Rax
involves: 129S/SvEv * C57BL/6J		 MGI:109527  MP:0003862 decreased aggression towards males PMID: 11972034 
Trpc2Gt(OST448844)Lex Trpc2Gt(OST448844)Lex/Trpc2Gt(OST448844)Lex
involves: 129S5/SvEvBrd		 MGI:109527  MP:0005178 increased circulating cholesterol level
Trpc2Gt(OST448844)Lex Trpc2Gt(OST448844)Lex/Trpc2Gt(OST448844)Lex
involves: 129S5/SvEvBrd		 MGI:109527  MP:0001552 increased circulating triglyceride level
Trpc2tm1Rax Trpc2tm1Rax/Trpc2tm1Rax
involves: 129S/SvEv * C57BL/6J		 MGI:109527  MP:0001355 submission towards male mice PMID: 11972034 
Trpc2tm1Dlc Trpc2tm1Dlc/Trpc2tm1Dlc
involves: 129S1/Sv * C57BL/6		 MGI:109527  MP:0001355 submission towards male mice PMID: 11823606 
Biologically Significant Variants Click here for help
Type:  Splice variant
Species:  Mouse
Description:  Isoform 2, probably the functional splice variant in the VNO.
Amino acids:  890
Nucleotide accession:  NM_001109897
Protein accession:  NP_001103367
References:  3,12
Type:  Splice variant
Species:  Mouse
Description:  Isoform 1
Amino acids:  1264
Nucleotide accession:  NM_011644
Protein accession:  NP_035774
References:  3,12

References

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1. Brann JH, Dennis JC, Morrison EE, Fadool DA. (2002) Type-specific inositol 1,4,5-trisphosphate receptor localization in the vomeronasal organ and its interaction with a transient receptor potential channel, TRPC2. J Neurochem, 83 (6): 1452-60. [PMID:12472899]

2. Chu X, Tong Q, Cheung JY, Wozney J, Conrad K, Mazack V, Zhang W, Stahl R, Barber DL, Miller BA. (2004) Interaction of TRPC2 and TRPC6 in erythropoietin modulation of calcium influx. J Biol Chem, 279 (11): 10514-22. [PMID:14699131]

3. Hofmann T, Schaefer M, Schultz G, Gudermann T. (2000) Cloning, expression and subcellular localization of two novel splice variants of mouse transient receptor potential channel 2. Biochem J, 351 (Pt 1): 115-22. [PMID:10998353]

4. Liman ER, Corey DP, Dulac C. (1999) TRP2: a candidate transduction channel for mammalian pheromone sensory signaling. Proc Natl Acad Sci USA, 96 (10): 5791-6. [PMID:10318963]

5. Lucas P, Ukhanov K, Leinders-Zufall T, Zufall F. (2003) A diacylglycerol-gated cation channel in vomeronasal neuron dendrites is impaired in TRPC2 mutant mice: mechanism of pheromone transduction. Neuron, 40 (3): 551-61. [PMID:14642279]

6. Omura M, Mombaerts P. (2014) Trpc2-expressing sensory neurons in the main olfactory epithelium of the mouse. Cell Rep, 8 (2): 583-95. [PMID:25001287]

7. Spehr J, Hagendorf S, Weiss J, Spehr M, Leinders-Zufall T, Zufall F. (2009) Ca2+ -calmodulin feedback mediates sensory adaptation and inhibits pheromone-sensitive ion channels in the vomeronasal organ. J Neurosci, 29 (7): 2125-35. [PMID:19228965]

8. Stamboulian S, Moutin MJ, Treves S, Pochon N, Grunwald D, Zorzato F, De Waard M, Ronjat M, Arnoult C. (2005) Junctate, an inositol 1,4,5-triphosphate receptor associated protein, is present in rodent sperm and binds TRPC2 and TRPC5 but not TRPC1 channels. Dev Biol, 286 (1): 326-37. [PMID:16153633]

9. Stowers L, Holy TE, Meister M, Dulac C, Koentges G. (2002) Loss of sex discrimination and male-male aggression in mice deficient for TRP2. Science, 295 (5559): 1493-500. [PMID:11823606]

10. Sutton KA, Jungnickel MK, Wang Y, Cullen K, Lambert S, Florman HM. (2004) Enkurin is a novel calmodulin and TRPC channel binding protein in sperm. Dev Biol, 274 (2): 426-35. [PMID:15385169]

11. Tong Q, Chu X, Cheung JY, Conrad K, Stahl R, Barber DL, Mignery G, Miller BA. (2004) Erythropoietin-modulated calcium influx through TRPC2 is mediated by phospholipase Cgamma and IP3R. Am J Physiol, Cell Physiol, 287 (6): C1667-78. [PMID:15329338]

12. Vannier B, Peyton M, Boulay G, Brown D, Qin N, Jiang M, Zhu X, Birnbaumer L. (1999) Mouse trp2, the homologue of the human trpc2 pseudogene, encodes mTrp2, a store depletion-activated capacitative Ca2+ entry channel. Proc Natl Acad Sci USA, 96 (5): 2060-4. [PMID:10051594]

13. Yildirim E, Dietrich A, Birnbaumer L. (2003) The mouse C-type transient receptor potential 2 (TRPC2) channel: alternative splicing and calmodulin binding to its N terminus. Proc Natl Acad Sci USA, 100 (5): 2220-5. [PMID:12601176]

14. Yu CR. (2015) TRICK or TRP? What Trpc2(-/-) mice tell us about vomeronasal organ mediated innate behaviors. Front Neurosci, 9: 221. [PMID:26157356]

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