TRPM5
target has curated data in GtoImmuPdb
Target id: 497
Nomenclature: TRPM5
Family: Transient Receptor Potential channels
Annotation status:
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Contents:
- Gene and Protein Information
- Previous and Unofficial Names
- Database Links
- Functional Characteristics
- Ion Selectivity and Conductance
- Voltage Dependence
- Rank order lists
- Activators
- Channel Blockers
- Immunopharmacology Comments
- Tissue Distribution
- Functional Assays
- Physiological Functions
- Phenotypes, Alleles and Disease Models
- References
- Contributors
- How to cite this page
Gene and Protein Information  |
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| Species | TM | P Loops | AA | Chromosomal Location | Gene Symbol | Gene Name | Reference |
| Human | 6 | 1 | 1165 | 11p15.5 | TRPM5 | transient receptor potential cation channel subfamily M member 5 | 22 |
| Mouse | 6 | 1 | 1158 | 7 F5 | Trpm5 | transient receptor potential cation channel | 5 |
| Rat | 6 | 1 | 1158 | 1q42 | Trpm5 | transient receptor potential cation channel | |
| Previous and Unofficial Names |
| MTR1 | TRP-T | L-TRPC5 | transient receptor potential cation channel |
| Database Links |
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| CATH/Gene3D | 1.25.40.20 |
| ChEMBL Target | CHEMBL1628468 (Hs), CHEMBL3886124 (Mm) |
| Ensembl Gene | ENSG00000070985 (Hs), ENSMUSG00000009246 (Mm), ENSRNOG00000020484 (Rn) |
| Entrez Gene | 29850 (Hs), 56843 (Mm), 365391 (Rn) |
| Human Protein Atlas | ENSG00000070985 (Hs) |
| KEGG Gene | hsa:29850 (Hs), mmu:56843 (Mm), rno:365391 (Rn) |
| OMIM | 604600 (Hs) |
| Pharos | Q9NZQ8 (Hs) |
| RefSeq Nucleotide | NM_014555 (Hs), NM_020277 (Mm), NM_001191896 (Rn) |
| RefSeq Protein | NP_055370 (Hs), NP_064673 (Mm), NP_001178825 (Rn) |
| UniProtKB | Q9NZQ8 (Hs), Q9JJH7 (Mm) |
| Wikipedia | TRPM5 (Hs) |
| Functional Characteristics |
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| γ = 15-25 pS; conducts monovalent cations selectively (PCa/PNa = 0.05); strong outward rectification; slow activation at positive potentials, rapid inactivation at negative potentials; activated and subsequently desensitized by [Ca2+]I | |
| Ion Selectivity and Conductance Comments |
| Nonselective for monovalent cations, impermeable to divalent cations. Average single-channel conductance of mTRPM5 is 20-25 pS [8,23]. Instantaneous single-channel conductance is linear [23]. |
| Voltage Dependence |
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| Physical activators (Human) |
| membrane depolarization (V½ = 0 to + 120 mV dependent upon conditions), heat (Q10 = 10.3 @ -75 mV between 15 and 25°C) |
Download all structure-activity data for this target as a CSV file 
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| Activator Comments | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Desensitisation of TRPM5 is modulated by PIP2, which acts as a co-factor for activation [14]. Human TRPM5 expressed in HEK293 cells is sensitive to rapid but not slow elevations in Ca2+ [23]. Arachidonic acid (10µM) activates mouse Trpm5 in transfected HEK293 cells [20]. Flash photolysis of caged IP3 activates mTrpm5 current in whole-cell but not excised patches [30]. In a calcium uncaging experiment mouse Trpm5 is activated with a time constant (τ) of 1.9ms at 100mV [28]. |
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| Channel Blocker Comments | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| TRPM5 is sensitive to pH below 7.0, with complete block at around pH 5.9 [15]. Histidines in the pore region (His-934) and a glutamate in the S3-S4 extracellular linker (Glu-830) enhance sensitivity to proton block [15]. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunopharmacology Comments |
| Expressed on mouse B cells [21]. |
| Tissue Distribution
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| Functional Assays
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| Physiological Functions
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| Phenotypes, Alleles and Disease Models
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Mouse data from MGI | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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References
1. Bezençon C, le Coutre J, Damak S. (2007) Taste-signaling proteins are coexpressed in solitary intestinal epithelial cells. Chem. Senses, 32 (1): 41-9. [PMID:17030556]
2. Brixel LR, Monteilh-Zoller MK, Ingenbrandt CS, Fleig A, Penner R, Enklaar T, Zabel BU, Prawitt D. (2010) TRPM5 regulates glucose-stimulated insulin secretion. Pflugers Arch., 460 (1): 69-76. [PMID:20393858]
3. Colsoul B, Schraenen A, Lemaire K, Quintens R, Van Lommel L, Segal A, Owsianik G, Talavera K, Voets T, Margolskee RF et al.. (2010) Loss of high-frequency glucose-induced Ca2+ oscillations in pancreatic islets correlates with impaired glucose tolerance in Trpm5-/- mice. Proc. Natl. Acad. Sci. U.S.A., 107 (11): 5208-13. [PMID:20194741]
4. Damak S, Rong M, Yasumatsu K, Kokrashvili Z, Pérez CA, Shigemura N, Yoshida R, Mosinger Jr B, Glendinning JI, Ninomiya Y et al.. (2006) Trpm5 null mice respond to bitter, sweet, and umami compounds. Chem. Senses, 31 (3): 253-64. [PMID:16436689]
5. Enklaar T, Esswein M, Oswald M, Hilbert K, Winterpacht A, Higgins M, Zabel B, Prawitt D. (2000) Mtr1, a novel biallelically expressed gene in the center of the mouse distal chromosome 7 imprinting cluster, is a member of the Trp gene family. Genomics, 67 (2): 179-87. [PMID:10903843]
6. Fonfria E, Murdock PR, Cusdin FS, Benham CD, Kelsell RE, McNulty S. (2006) Tissue distribution profiles of the human TRPM cation channel family. J. Recept. Signal Transduct. Res., 26 (3): 159-78. [PMID:16777713]
7. Hansen A, Finger TE. (2008) Is TrpM5 a reliable marker for chemosensory cells? Multiple types of microvillous cells in the main olfactory epithelium of mice. BMC Neurosci, 9: 115. [PMID:19055837]
8. Hofmann T, Chubanov V, Gudermann T, Montell C. (2003) TRPM5 is a voltage-modulated and Ca(2+)-activated monovalent selective cation channel. Curr. Biol., 13 (13): 1153-8. [PMID:12842017]
9. Huang YA, Roper SD. (2010) Intracellular Ca(2+) and TRPM5-mediated membrane depolarization produce ATP secretion from taste receptor cells. J. Physiol. (Lond.), 588 (Pt 13): 2343-50. [PMID:20498227]
10. Kim YS, Kang E, Makino Y, Park S, Shin JH, Song H, Launay P, Linden DJ. (2013) Characterizing the conductance underlying depolarization-induced slow current in cerebellar Purkinje cells. J. Neurophysiol., 109 (4): 1174-81. [PMID:23197456]
11. Lin W, Ezekwe Jr EA, Zhao Z, Liman ER, Restrepo D. (2008) TRPM5-expressing microvillous cells in the main olfactory epithelium. BMC Neurosci, 9: 114. [PMID:19025635]
12. Lin W, Margolskee R, Donnert G, Hell SW, Restrepo D. (2007) Olfactory neurons expressing transient receptor potential channel M5 (TRPM5) are involved in sensing semiochemicals. Proc. Natl. Acad. Sci. U.S.A., 104 (7): 2471-6. [PMID:17267604]
13. Lin W, Ogura T, Margolskee RF, Finger TE, Restrepo D. (2008) TRPM5-expressing solitary chemosensory cells respond to odorous irritants. J. Neurophysiol., 99 (3): 1451-60. [PMID:18160424]
14. Liu D, Liman ER. (2003) Intracellular Ca2+ and the phospholipid PIP2 regulate the taste transduction ion channel TRPM5. Proc. Natl. Acad. Sci. U.S.A., 100 (25): 15160-5. [PMID:14657398]
15. Liu D, Zhang Z, Liman ER. (2005) Extracellular acid block and acid-enhanced inactivation of the Ca2+-activated cation channel TRPM5 involve residues in the S3-S4 and S5-S6 extracellular domains. J. Biol. Chem., 280 (21): 20691-9. [PMID:15731110]
16. Liu P, Shah BP, Croasdell S, Gilbertson TA. (2011) Transient receptor potential channel type M5 is essential for fat taste. J. Neurosci., 31 (23): 8634-42. [PMID:21653867]
17. Mitrovic S, Nogueira C, Cantero-Recasens G, Kiefer K, Fernández-Fernández JM, Popoff JF, Casano L, Bard FA, Gomez R, Valverde MA et al.. (2013) TRPM5-mediated calcium uptake regulates mucin secretion from human colon goblet cells. Elife, 2: e00658. [PMID:23741618]
18. Nilius B, Prenen J, Janssens A, Owsianik G, Wang C, Zhu MX, Voets T. (2005) The selectivity filter of the cation channel TRPM4. J. Biol. Chem., 280 (24): 22899-906. [PMID:15845551]
19. Nilius B, Prenen J, Janssens A, Voets T, Droogmans G. (2004) Decavanadate modulates gating of TRPM4 cation channels. J. Physiol. (Lond.), 560 (Pt 3): 753-65. [PMID:15331675]
20. Oike H, Wakamori M, Mori Y, Nakanishi H, Taguchi R, Misaka T, Matsumoto I, Abe K. (2006) Arachidonic acid can function as a signaling modulator by activating the TRPM5 cation channel in taste receptor cells. Biochim. Biophys. Acta, 1761 (9): 1078-84. [PMID:16935556]
21. Parenti A, De Logu F, Geppetti P, Benemei S. (2016) What is the evidence for the role of TRP channels in inflammatory and immune cells?. Br. J. Pharmacol., 173 (6): 953-69. [PMID:26603538]
22. Prawitt D, Enklaar T, Klemm G, Gärtner B, Spangenberg C, Winterpacht A, Higgins M, Pelletier J, Zabel B. (2000) Identification and characterization of MTR1, a novel gene with homology to melastatin (MLSN1) and the trp gene family located in the BWS-WT2 critical region on chromosome 11p15.5 and showing allele-specific expression. Hum. Mol. Genet., 9 (2): 203-16. [PMID:10607831]
23. Prawitt D, Monteilh-Zoller MK, Brixel L, Spangenberg C, Zabel B, Fleig A, Penner R. (2003) TRPM5 is a transient Ca2+-activated cation channel responding to rapid changes in [Ca2+]i. Proc. Natl. Acad. Sci. U.S.A., 100 (25): 15166-71. [PMID:14634208]
24. Pérez CA, Huang L, Rong M, Kozak JA, Preuss AK, Zhang H, Max M, Margolskee RF. (2002) A transient receptor potential channel expressed in taste receptor cells. Nat. Neurosci., 5 (11): 1169-76. [PMID:12368808]
25. Talavera K, Yasumatsu K, Voets T, Droogmans G, Shigemura N, Ninomiya Y, Margolskee RF, Nilius B. (2005) Heat activation of TRPM5 underlies thermal sensitivity of sweet taste. Nature, 438 (7070): 1022-5. [PMID:16355226]
26. Teruyama R, Sakuraba M, Kurotaki H, Armstrong WE. (2011) Transient receptor potential channel m4 and m5 in magnocellular cells in rat supraoptic and paraventricular nuclei. J. Neuroendocrinol., 23 (12): 1204-13. [PMID:21848647]
27. Tizzano M, Gulbransen BD, Vandenbeuch A, Clapp TR, Herman JP, Sibhatu HM, Churchill ME, Silver WL, Kinnamon SC, Finger TE. (2010) Nasal chemosensory cells use bitter taste signaling to detect irritants and bacterial signals. Proc. Natl. Acad. Sci. U.S.A., 107 (7): 3210-5. [PMID:20133764]
28. Ullrich ND, Voets T, Prenen J, Vennekens R, Talavera K, Droogmans G, Nilius B. (2005) Comparison of functional properties of the Ca2+-activated cation channels TRPM4 and TRPM5 from mice. Cell Calcium, 37 (3): 267-78. [PMID:15670874]
29. Zhang Y, Hoon MA, Chandrashekar J, Mueller KL, Cook B, Wu D, Zuker CS, Ryba NJ. (2003) Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways. Cell, 112 (3): 293-301. [PMID:12581520]
30. Zhang Z, Zhao Z, Margolskee R, Liman E. (2007) The transduction channel TRPM5 is gated by intracellular calcium in taste cells. J. Neurosci., 27 (21): 5777-86. [PMID:17522321]
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