TRPV5
target has curated data in GtoImmuPdb
Target id: 511
Nomenclature: TRPV5
Family: Transient Receptor Potential channels
Annotation status:
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Contents:
- Gene and Protein Information
- Previous and Unofficial Names
- Database Links
- Associated Proteins
- Functional Characteristics
- Ion Selectivity and Conductance
- Voltage Dependence
- Rank order lists
- Activators
- Channel Blockers
- Immunopharmacology Comments
- Tissue Distribution
- Functional Assays
- Physiological Functions
- Physiological Consequences of Altering Gene Expression
- Phenotypes, Alleles and Disease Models
- References
- Contributors
- How to cite this page
Gene and Protein Information  |
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| Species | TM | P Loops | AA | Chromosomal Location | Gene Symbol | Gene Name | Reference |
| Human | 6 | 1 | 729 | 7q35 | TRPV5 | transient receptor potential cation channel subfamily V member 5 | 11 |
| Mouse | 6 | 1 | 723 | 6 B2.1 | Trpv5 | transient receptor potential cation channel | 26 |
| Rat | 6 | 1 | 723 | 4q23 | Trpv5 | transient receptor potential cation channel | 15-16 |
| Previous and Unofficial Names |
| OTRPC3 | ECaC | ECaC1 | epithelial calcium channel 1 | calcium transporter 2 | osm-9-like TRP channel 3 | CAT2 | transient receptor potential cation channel |
| Database Links |
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| CATH/Gene3D | 1.25.40.20 |
| Ensembl Gene | ENSG00000127412 (Hs), ENSMUSG00000036899 (Mm), ENSRNOG00000015394 (Rn) |
| Entrez Gene | 56302 (Hs), 194352 (Mm), 116469 (Rn) |
| Human Protein Atlas | ENSG00000127412 (Hs) |
| KEGG Gene | hsa:56302 (Hs), mmu:194352 (Mm), rno:116469 (Rn) |
| OMIM | 606679 (Hs) |
| Pharos | Q9NQA5 (Hs) |
| RefSeq Nucleotide | NM_019841 (Hs), NM_001007572 (Mm), NM_053787 (Rn) |
| RefSeq Protein | NP_062815 (Hs), NP_001007573 (Mm), NP_446239 (Rn) |
| UniProtKB | Q9NQA5 (Hs), P69744 (Mm), Q9JIP0 (Rn) |
| Wikipedia | TRPV5 (Hs) |
| Associated Proteins |
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| Functional Characteristics |
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| γ = 59–78 pS for monovalent ions at negative potentials, conducts mono- and di-valents with high selectivity for divalents (PCa/PNa > 107); voltage- and time- dependent inward rectification; inhibited by intracellular Ca2+ promoting fast inactivation and slow downregulation; feedback inhibition by Ca2+ reduced by calcium binding protein 80-K-H; inhibited by extracellular and intracellular acidosis; upregulated by 1,25-dihydrovitamin D3 | |
| Ion Selectivity and Conductance Comments |
| At present the only ion selectivity data available for TRPV5 is from rabbit studies. Ion selectivity is ordered Na+ > Li+ > K+ > Cs+ > NMDG+ (N-methyl-G-glucamine) [13], and Ca2+ > Ba2+ = Sr2+ > Mn2+ [24]. Overall single channel conductance is 72-82 pS [13]. Subject to Mg2+ block. PCa:PNa ratio > 100 [13,24]. |
| Voltage Dependence Comments |
| Blocking by intracellular Mg2+ displays voltage dependency [13]. |
| Activators (Human) |
| constitutively active (with strong buffering of intracellular Ca2+) |
| Other channel blockers (Human) |
| Pb2+ = Cu2+ = Gd3+ > Cd2+ > Zn2+ > La3+ > Co2+ > Fe2 |
Download all structure-activity data for this target as a CSV file 
| Activator Comments | ||
| TRPV5 is constitutively active [24]. Its function is modulated by extracellular protons [25,27-28]. Parathyroid hormone, 17β-estradiol and 1,25-dihydroxyvitamin D(3) activate TRPV5 by upregulating its expression level as well as directly activating the channel [2,4,7,17]. Klotho increases plasma membrane TRPV5 abundance by hydrolysis of its extracellular N-linked oligosaccharides [1]. |
| Channel Blockers | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Key to terms and symbols | View all chemical structures | Click column headers to sort | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| View species-specific channel blocker tables | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunopharmacology Comments |
| Expressed on human T cells [14]. |
| Tissue Distribution
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| Functional Assays
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| Physiological Functions
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| Physiological Consequences of Altering Gene Expression
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| Phenotypes, Alleles and Disease Models
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References
1. Chang Q, Hoefs S, van der Kemp AW, Topala CN, Bindels RJ, Hoenderop JG. (2005) The beta-glucuronidase klotho hydrolyzes and activates the TRPV5 channel. Science, 310 (5747): 490-3. [PMID:16239475]
2. de Groot T, Lee K, Langeslag M, Xi Q, Jalink K, Bindels RJ, Hoenderop JG. (2009) Parathyroid hormone activates TRPV5 via PKA-dependent phosphorylation. J. Am. Soc. Nephrol., 20 (8): 1693-704. [PMID:19423690]
3. Gkika D, Mahieu F, Nilius B, Hoenderop JG, Bindels RJ. (2004) 80K-H as a new Ca2+ sensor regulating the activity of the epithelial Ca2+ channel transient receptor potential cation channel V5 (TRPV5). J. Biol. Chem., 279 (25): 26351-7. [PMID:15100231]
4. Hoenderop JG, Müller D, Van Der Kemp AW, Hartog A, Suzuki M, Ishibashi K, Imai M, Sweep F, Willems PH, Van Os CH et al.. (2001) Calcitriol controls the epithelial calcium channel in kidney. J. Am. Soc. Nephrol., 12 (7): 1342-9. [PMID:11423563]
5. Hoenderop JG, van Leeuwen JP, van der Eerden BC, Kersten FF, van der Kemp AW, Mérillat AM, Waarsing JH, Rossier BC, Vallon V, Hummler E, Bindels RJ. (2003) Renal Ca2+ wasting, hyperabsorption, and reduced bone thickness in mice lacking TRPV5. J. Clin. Invest., 112 (12): 1906-14. [PMID:14679186]
6. Hoenderop JG, Voets T, Hoefs S, Weidema F, Prenen J, Nilius B, Bindels RJ. (2003) Homo- and heterotetrameric architecture of the epithelial Ca2+ channels TRPV5 and TRPV6. EMBO J., 22 (4): 776-85. [PMID:12574114]
7. Irnaten M, Blanchard-Gutton N, Praetorius J, Harvey BJ. (2009) Rapid effects of 17beta-estradiol on TRPV5 epithelial Ca2+ channels in rat renal cells. Steroids, 74 (8): 642-9. [PMID:19463684]
8. Janssen SW, Hoenderop JG, Hermus AR, Sweep FC, Martens GJ, Bindels RJ. (2002) Expression of the novel epithelial Ca2+ channel ECaC1 in rat pancreatic islets. J. Histochem. Cytochem., 50 (6): 789-98. [PMID:12019295]
9. Lambers TT, Mahieu F, Oancea E, Hoofd L, de Lange F, Mensenkamp AR, Voets T, Nilius B, Clapham DE, Hoenderop JG et al.. (2006) Calbindin-D28K dynamically controls TRPV5-mediated Ca2+ transport. EMBO J., 25 (13): 2978-88. [PMID:16763551]
10. Lambers TT, Weidema AF, Nilius B, Hoenderop JG, Bindels RJ. (2004) Regulation of the mouse epithelial Ca2(+) channel TRPV6 by the Ca(2+)-sensor calmodulin. J. Biol. Chem., 279 (28): 28855-61. [PMID:15123711]
11. Müller D, Hoenderop JG, Meij IC, van den Heuvel LP, Knoers NV, den Hollander AI, Eggert P, García-Nieto V, Claverie-Martín F, Bindels RJ. (2000) Molecular cloning, tissue distribution, and chromosomal mapping of the human epithelial Ca2+ channel (ECAC1). Genomics, 67 (1): 48-53. [PMID:10945469]
12. Nilius B, Prenen J, Vennekens R, Hoenderop JG, Bindels RJ, Droogmans G. (2001) Pharmacological modulation of monovalent cation currents through the epithelial Ca2+ channel ECaC1. Br. J. Pharmacol., 134 (3): 453-62. [PMID:11588099]
13. Nilius B, Vennekens R, Prenen J, Hoenderop JG, Bindels RJ, Droogmans G. (2000) Whole-cell and single channel monovalent cation currents through the novel rabbit epithelial Ca2+ channel ECaC. J. Physiol. (Lond.), 527 Pt 2: 239-48. [PMID:10970426]
14. Parenti A, De Logu F, Geppetti P, Benemei S. (2016) What is the evidence for the role of TRP channels in inflammatory and immune cells?. Br. J. Pharmacol., 173 (6): 953-69. [PMID:26603538]
15. Peng JB, Chen XZ, Berger UV, Vassilev PM, Brown EM, Hediger MA. (2000) A rat kidney-specific calcium transporter in the distal nephron. J. Biol. Chem., 275 (36): 28186-94. [PMID:10875938]
16. Suzuki M, Ishibashi K, Ooki G, Tsuruoka S, Imai M. (2000) Electrophysiologic characteristics of the Ca-permeable channels, ECaC and CaT, in the kidney. Biochem. Biophys. Res. Commun., 274 (2): 344-9. [PMID:10913341]
17. Van Abel M, Hoenderop JG, Dardenne O, St Arnaud R, Van Os CH, Van Leeuwen HJ, Bindels RJ. (2002) 1,25-dihydroxyvitamin D(3)-independent stimulatory effect of estrogen on the expression of ECaC1 in the kidney. J. Am. Soc. Nephrol., 13 (8): 2102-9. [PMID:12138142]
18. van Abel M, Hoenderop JG, van der Kemp AW, Friedlaender MM, van Leeuwen JP, Bindels RJ. (2005) Coordinated control of renal Ca(2+) transport proteins by parathyroid hormone. Kidney Int., 68 (4): 1708-21. [PMID:16164647]
19. van de Graaf SF, Chang Q, Mensenkamp AR, Hoenderop JG, Bindels RJ. (2006) Direct interaction with Rab11a targets the epithelial Ca2+ channels TRPV5 and TRPV6 to the plasma membrane. Mol. Cell. Biol., 26 (1): 303-12. [PMID:16354700]
20. van de Graaf SF, Hoenderop JG, Gkika D, Lamers D, Prenen J, Rescher U, Gerke V, Staub O, Nilius B, Bindels RJ. (2003) Functional expression of the epithelial Ca(2+) channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complex. EMBO J., 22 (7): 1478-87. [PMID:12660155]
21. van de Graaf SF, Hoenderop JG, van der Kemp AW, Gisler SM, Bindels RJ. (2006) Interaction of the epithelial Ca2+ channels TRPV5 and TRPV6 with the intestine- and kidney-enriched PDZ protein NHERF4. Pflugers Arch., 452 (4): 407-17. [PMID:16565876]
22. van de Graaf SF, van der Kemp AW, van den Berg D, van Oorschot M, Hoenderop JG, Bindels RJ. (2006) Identification of BSPRY as a novel auxiliary protein inhibiting TRPV5 activity. J. Am. Soc. Nephrol., 17 (1): 26-30. [PMID:16380433]
23. van der Eerden BC, Hoenderop JG, de Vries TJ, Schoenmaker T, Buurman CJ, Uitterlinden AG, Pols HA, Bindels RJ, van Leeuwen JP. (2005) The epithelial Ca2+ channel TRPV5 is essential for proper osteoclastic bone resorption. Proc. Natl. Acad. Sci. U.S.A., 102 (48): 17507-12. [PMID:16291808]
24. Vennekens R, Hoenderop JG, Prenen J, Stuiver M, Willems PH, Droogmans G, Nilius B, Bindels RJ. (2000) Permeation and gating properties of the novel epithelial Ca(2+) channel. J. Biol. Chem., 275 (6): 3963-9. [PMID:10660551]
25. Vennekens R, Prenen J, Hoenderop JG, Bindels RJ, Droogmans G, Nilius B. (2001) Modulation of the epithelial Ca2+ channel ECaC by extracellular pH. Pflugers Arch., 442 (2): 237-42. [PMID:11417220]
26. Weber K, Erben RG, Rump A, Adamski J. (2001) Gene structure and regulation of the murine epithelial calcium channels ECaC1 and 2. Biochem. Biophys. Res. Commun., 289 (5): 1287-94. [PMID:11741335]
27. Yeh BI, Kim YK, Jabbar W, Huang CL. (2005) Conformational changes of pore helix coupled to gating of TRPV5 by protons. EMBO J., 24 (18): 3224-34. [PMID:16121193]
28. Yeh BI, Sun TJ, Lee JZ, Chen HH, Huang CL. (2003) Mechanism and molecular determinant for regulation of rabbit transient receptor potential type 5 (TRPV5) channel by extracellular pH. J. Biol. Chem., 278 (51): 51044-52. [PMID:14525991]
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