ChEMBL ligand: CHEMBL256147 (L-NMMA, Ng-methylarginine, Ng-monomethyl-l-arginine, .omega.-n-methylarginine, Omega-n-methylarginine, Omega-n-methylated arginine, Targinine, Tilarginine)
Neuronal NOS/Nitric-oxide synthase, brain in Human [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475] Neuronal NOS/Nitric-oxide synthase, brain in Mouse [ChEMBL: CHEMBL4719] [GtoPdb: 1251] [UniProtKB: Q9Z0J4] Neuronal NOS/Nitric-oxide synthase, brain in Rat [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476] There should be some charts here, you may need to enable JavaScript!
Endothelial NOS/Nitric-oxide synthase, endothelial in Human [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474] Nitric-oxide synthase, endothelial in Bovine [ChEMBL: CHEMBL4802] [UniProtKB: P29473] There should be some charts here, you may need to enable JavaScript!
Inducible NOS/Nitric oxide synthase, inducible in Human [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228] Inducible NOS/Nitric oxide synthase, inducible in Mouse [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Neuronal NOS/Nitric-oxide synthase, brain in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475]
ChEMBL	Inhibitory constant for the inhibition of human Neuronal nitric oxide synthase	B	6.08	pKi	840	nM	Ki	Bioorg Med Chem Lett (1997) 7: 1763-1768
ChEMBL	Inhibitory activity against human neuronal nitric oxide synthase (nNOS) isoenzyme.	B	6.08	pKi	840	nM	Ki	J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
GtoPdb	-	-	6.08	pKi	840	nM	Ki	Bioorganic and Medicinal Chemistry Letters (1997) 7: 1763-1768
ChEMBL	Inhibition of cloned (from RNA) human Neuronal nitric oxide synthase	B	5	pIC50	10000	nM	IC50	J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL	Inhibition of human neuronal Nitric Oxide Synthase	B	5.31	pIC50	4900	nM	IC50	Bioorg Med Chem Lett (2000) 10: 597-600 [PMID:10741561]
ChEMBL	Inhibitory activity against neuronal nitric oxide synthase	B	5.57	pIC50	2700	nM	IC50	Bioorg Med Chem Lett (2000) 10: 1975-1978 [PMID:10987430]
ChEMBL	Inhibition of human recombinant nNOS assessed as conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins measured after 45 mins by liquid scintillation counting	B	6.02	pIC50	950	nM	IC50	ACS Med Chem Lett (2012) 3: 227-231 [PMID:24900459]
ChEMBL	Inhibition of human nNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method	B	6.02	pIC50	950	nM	IC50	J Med Chem (2012) 55: 2882-2893 [PMID:22335555]
ChEMBL	Inhibitory concentration against recombinant human (neuronal nitric oxide synthase) n-NOS	B	6.05	pIC50	890	nM	IC50	Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
Neuronal NOS/Nitric-oxide synthase, brain in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4719] [GtoPdb: 1251] [UniProtKB: Q9Z0J4]
ChEMBL	Inhibition of neuronal nitric oxide synthase (nNOS) in mice	B	5.37	pIC50	4300	nM	IC50	Bioorg Med Chem Lett (2004) 14: 313-316 [PMID:14698148]
Neuronal NOS/Nitric-oxide synthase, brain in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476]
ChEMBL	Inhibition of rat nNOS	B	6.7	pKi	200	nM	Ki	J Med Chem (2013) 56: 3121-3147 [PMID:23458846]
ChEMBL	Compound was tested in vitro for inhibitory activity against nNOS (constitutive isoform found in neurons)	B	5	pIC50	10000	nM	IC50	Bioorg Med Chem Lett (1996) 6: 999-1002
ChEMBL	Inhibitory activity against isomeric form of Neuronal nitric oxide synthase measured by citrulline assay	B	5.12	pIC50	7500	nM	IC50	J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
ChEMBL	Ability to inhibit the conversion of [3H]-L-Arg to [3H]L-citrulline catalyzed by n-NOS from rat cerebellum	F	7	pIC50	100	nM	IC50	Bioorg Med Chem Lett (2001) 11: 1027-1030 [PMID:11327581]
ChEMBL	Inhibition of conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by neuronal NOS (n NOS) from rat cerebellum	F	7	pIC50	100	nM	IC50	Bioorg Med Chem Lett (2001) 11: 1023-1026 [PMID:11327580]
Endothelial NOS/Nitric-oxide synthase, endothelial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474]
ChEMBL	Inhibitory constant for the inhibition of human Endothelial nitric oxide synthase	B	6.4	pKi	400	nM	Ki	Bioorg Med Chem Lett (1997) 7: 1763-1768
ChEMBL	Inhibitory activity against human endothelial nitric oxide synthase (eNOS) isoenzyme.	B	6.4	pKi	400	nM	Ki	J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
GtoPdb	-	-	6.4	pKi	400	nM	Ki	Bioorganic and Medicinal Chemistry Letters (1997) 7: 1763-1768
ChEMBL	Inhibitory activity against endothelial nitric oxide synthase (eNOS)	B	5.06	pIC50	8700	nM	IC50	Bioorg Med Chem Lett (2000) 10: 1975-1978 [PMID:10987430]
ChEMBL	The compound was evaluated for the inhibitory potencies using human Endothelial nitric oxide synthase	B	5.22	pIC50	6000	nM	IC50	J Med Chem (1996) 39: 5110-5118 [PMID:8978842]
ChEMBL	Inhibition of cloned (from RNA) human endothelial constitutive Endothelial nitric oxide synthase (heNOS)	B	5.23	pIC50	5900	nM	IC50	J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL	Inhibition of human endothelial Nitric Oxide Synthase	B	5.46	pIC50	3500	nM	IC50	Bioorg Med Chem Lett (2000) 10: 597-600 [PMID:10741561]
ChEMBL	Inhibition of human eNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method	B	6.19	pIC50	650	nM	IC50	J Med Chem (2012) 55: 2882-2893 [PMID:22335555]
ChEMBL	Inhibition of human recombinant eNOS assessed as conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins measured after 45 mins by liquid scintillation counting	B	6.19	pIC50	650	nM	IC50	ACS Med Chem Lett (2012) 3: 227-231 [PMID:24900459]
ChEMBL	Inhibitory concentration against recombinant human Endothelial nitric oxide synthase	B	6.27	pIC50	540	nM	IC50	Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL	Ability to inhibit the conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by e-NOS from HUVEC cells	F	6.52	pIC50	300	nM	IC50	Bioorg Med Chem Lett (2001) 11: 1027-1030 [PMID:11327581]
ChEMBL	Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by endothelial NOS (e NOS) from HUVEC cells	F	6.52	pIC50	300	nM	IC50	Bioorg Med Chem Lett (2001) 11: 1023-1026 [PMID:11327580]
Nitric-oxide synthase, endothelial in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4802] [UniProtKB: P29473]
ChEMBL	Inhibitory activity of conversion of radiolabeled arginine to citrulline by isomeric form of nitric oxide synthase Endothelial nitric oxide synthase from cultured bovine aortic endothelial cells	B	5.15	pIC50	7000	nM	IC50	J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
Inducible NOS/Nitric oxide synthase, inducible in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228]
ChEMBL	Inhibitory constant for the inhibition of human Inducible nitric oxide synthase	B	6.07	pKi	860	nM	Ki	Bioorg Med Chem Lett (1997) 7: 1763-1768
ChEMBL	Inhibitory activity against human inducible nitric oxide synthase (iNOS) isoenzyme.	B	6.07	pKi	860	nM	Ki	J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
GtoPdb	-	-	6.07	pKi	860	nM	Ki	Bioorganic and Medicinal Chemistry Letters (1997) 7: 1763-1768
ChEMBL	The compound was evaluated for the inhibitory potencies using human Inducible nitric oxide synthase (hiNOS)	B	4.57	pIC50	27000	nM	IC50	J Med Chem (1996) 39: 5110-5118 [PMID:8978842]
ChEMBL	Inhibition of cloned (from RNA) human inducible nitric oxide synthase (hiNOS)	B	4.85	pIC50	14000	nM	IC50	J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL	Inhibitory activity against inducible nitric oxide synthase (iNOS)	B	5	pIC50	10000	nM	IC50	Bioorg Med Chem Lett (2000) 10: 1975-1978 [PMID:10987430]
ChEMBL	Inhibition of human inducible nitric oxide synthase	B	5.18	pIC50	6600	nM	IC50	Bioorg Med Chem Lett (2000) 10: 597-600 [PMID:10741561]
ChEMBL	Inhibition of iNOS	B	5.47	pIC50	3400	nM	IC50	J Med Chem (2008) 51: 4150-4169 [PMID:18588282]
ChEMBL	Inhibition of human recombinant iNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method	B	5.74	pIC50	1800	nM	IC50	J Med Chem (2012) 55: 2882-2893 [PMID:22335555]
ChEMBL	Inhibitory concentration against human Inducible nitric oxide synthase	B	6	pIC50	1000	nM	IC50	Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL	Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by inducible NOS (i NOS) from human DLD-1 cells	F	6.52	pIC50	300	nM	IC50	Bioorg Med Chem Lett (2001) 11: 1023-1026 [PMID:11327580]
ChEMBL	Ability to inhibit the conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by i-NOS from human DLD-1 cells	F	6.52	pIC50	300	nM	IC50	Bioorg Med Chem Lett (2001) 11: 1027-1030 [PMID:11327581]
ChEMBL	Inhibition of human recombinant iNOS assessed as conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins measured after 45 mins by liquid scintillation counting	B	8.74	pIC50	1.8	nM	IC50	ACS Med Chem Lett (2012) 3: 227-231 [PMID:24900459]
Inducible NOS/Nitric oxide synthase, inducible in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477]
ChEMBL	Compound was tested in vitro for inhibitory activity against inducible nitric oxide synthase	B	4.6	pIC50	25000	nM	IC50	Bioorg Med Chem Lett (1996) 6: 999-1002
ChEMBL	Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells	B	4.6	pIC50	25000	nM	IC50	J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
ChEMBL	Inhibition of iNOS-mediated nitric oxide production in LPS-stimulated mouse RAW 264.7 cells pretreated 15 mins before LPS challenge measured after 20 hrs relative to control	B	4.66	pIC50	22100	nM	IC50	Bioorg Med Chem (2011) 19: 6182-6195 [PMID:21978950]
ChEMBL	Inhibition of LPS-induced iNOS activity in mouse RAW 264.7 cells assessed as inhibition of NO production pretreated with compound for 30 mins before LPS challenge after 24 hrs by Griess reagent method	B	4.71	pIC50	19700	nM	IC50	Bioorg Med Chem (2012) 20: 7030-7039 [PMID:23142320]
ChEMBL	Inhibition of inducible nitric oxide synthase (iNOS) in mice	B	4.72	pIC50	19000	nM	IC50	Bioorg Med Chem Lett (2004) 14: 313-316 [PMID:14698148]
ChEMBL	Inhibition of iNOS in ddY mouse peritoneal exudate cells after 20 hrs	B	4.89	pIC50	13000	nM	IC50	J Nat Prod (2004) 67: 1119-1124 [PMID:15270564]
ChEMBL	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced iNOS activity incubated for 30 mins prior to LPS challenge measured after 24 hrs by Griess assay	B	5.64	pIC50	2300	nM	IC50	Bioorg Med Chem (2015) 23: 3248-3259 [PMID:25975638]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]