ChEMBL ligand: CHEMBL295416 (NSC-310038, Pirinixic acid, WY-14643)
aldo-keto reductase family 1 member B/Aldose reductase in Rat [ChEMBL: CHEMBL2622] [GtoPdb: 2768] [UniProtKB: P07943] There should be some charts here, you may need to enable JavaScript!
Cyclooxygenase-1 in Sheep [ChEMBL: CHEMBL2949] [UniProtKB: P05979] There should be some charts here, you may need to enable JavaScript!
COX-2 /Cyclooxygenase-2 in Human [ChEMBL: CHEMBL230] [GtoPdb: 1376] [UniProtKB: P35354] There should be some charts here, you may need to enable JavaScript!
Peroxisome proliferator-activated receptor-α/Peroxisome proliferator-activated receptor alpha in Human [ChEMBL: CHEMBL239] [GtoPdb: 593] [UniProtKB: Q07869] Peroxisome proliferator-activated receptor-α/Peroxisome proliferator-activated receptor alpha in Mouse [ChEMBL: CHEMBL2128] [GtoPdb: 593] [UniProtKB: P23204] Peroxisome proliferator-activated receptor-α/Peroxisome proliferator-activated receptor alpha in Rat [ChEMBL: CHEMBL2129] [GtoPdb: 593] [UniProtKB: P37230] There should be some charts here, you may need to enable JavaScript!
Peroxisome proliferator-activated receptor-β/δ/Peroxisome proliferator-activated receptor delta in Human [ChEMBL: CHEMBL3979] [GtoPdb: 594] [UniProtKB: Q03181] There should be some charts here, you may need to enable JavaScript!
Peroxisome proliferator-activated receptor-γ/Peroxisome proliferator-activated receptor gamma in Human [ChEMBL: CHEMBL235] [GtoPdb: 595] [UniProtKB: P37231] Peroxisome proliferator-activated receptor-γ/Peroxisome proliferator-activated receptor gamma in Mouse [ChEMBL: CHEMBL2459] [GtoPdb: 595] [UniProtKB: P37238] There should be some charts here, you may need to enable JavaScript!
Retinoid X receptor-α/Retinoid X receptor alpha in Human [ChEMBL: CHEMBL2061] [GtoPdb: 610] [UniProtKB: P19793] There should be some charts here, you may need to enable JavaScript!
Retinoid X receptor-β/Retinoid X receptor beta in Human [ChEMBL: CHEMBL1870] [GtoPdb: 611] [UniProtKB: P28702] There should be some charts here, you may need to enable JavaScript!
Retinoid X receptor-γ/Retinoid X receptor gamma in Human [ChEMBL: CHEMBL2004] [GtoPdb: 612] [UniProtKB: P48443] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
aldo-keto reductase family 1 member B/Aldose reductase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2622] [GtoPdb: 2768] [UniProtKB: P07943]
ChEMBL	DRUGMATRIX: Aldose Reductase enzyme inhibition (substrate: DL-Glyceraldehyde)	B	5.55	pIC50	2816.9	nM	IC50	DrugMatrix in vitro pharmacology data
Cyclooxygenase-1 in Sheep (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2949] [UniProtKB: P05979]
ChEMBL	Inhibition of ovine COX1	B	8	pIC50	>10	nM	IC50	J. Med. Chem. (2008) 51: 8068-8076 [PMID:19053751]
COX-2 /Cyclooxygenase-2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL230] [GtoPdb: 1376] [UniProtKB: P35354]
ChEMBL	Inhibition of human recombinant COX2	B	8	pIC50	>10	nM	IC50	J. Med. Chem. (2008) 51: 8068-8076 [PMID:19053751]
Peroxisome proliferator-activated receptor-α/Peroxisome proliferator-activated receptor alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL239] [GtoPdb: 593] [UniProtKB: Q07869]
ChEMBL	Binding affinity to PPARalpha (unknown origin) assessed as thermodynamic dissociation constant by SPR assay	B	7.72	pKd	18.9	nM	Kd	Eur J Med Chem (2017) 127: 379-397 [PMID:28076827]
ChEMBL	Binding affinity to human recombinant PPARalpha by Cheng-Prusoff equation based competitive binding TR-FRET assay	B	5.35	pKi	4500	nM	Ki	Bioorg Med Chem (2018) 26: 5654-5663 [PMID:30352713]
ChEMBL	Binding affinity to PPARalpha (unknown origin) by TR-FRET assay	B	5.38	pKi	4130	nM	Ki	Bioorg Med Chem (2019) 27: 2948-2958 [PMID:31128991]
ChEMBL	Activation of human PPARalpha ligand binding domain expressed in COS7 cells by luciferase reporter gene assay	B	4.44	pIC50	36300	nM	IC50	Bioorg. Med. Chem. Lett. (2014) 24: 3757-3763 [PMID:25037914]
ChEMBL	Binding affinity to GST-tagged human PPARalpha by TR-FRET analysis	B	5	pIC50	10110	nM	IC50	Bioorg. Med. Chem. Lett. (2012) 22: 4122-4126 [PMID:22579420]
ChEMBL	Agonist activity at wild-type human PPARalpha LBD expressed in COS7 cells after 12 hrs by Dual-Glo luciferase assay	B	4.4	pEC50	39800	nM	EC50	Bioorg. Med. Chem. Lett. (2014) 24: 4048-4052 [PMID:25022880]
ChEMBL	Agonist activity at recombinant human PPARalpha LBD (168 to 468 residues) expressed in African green monkey COS-1 cells incubated for 18 hrs by luciferase reporter gene assay	B	4.41	pEC50	39000	nM	EC50	Eur J Med Chem (2018) 155: 736-753 [PMID:29940464]
ChEMBL	Activation of human Gal4-fused PPARalpha LBD transfected in HEK293T after 14 to 16 hrs by dual-glo luciferase reporter gene assay	B	4.44	pEC50	36000	nM	EC50	J Med Chem (2019) 62: 2112-2126 [PMID:30702885]
ChEMBL	Effective concentration for human peroxisome proliferator-activated receptor gamma	B	4.54	pEC50	29000	nM	EC50	Bioorg. Med. Chem. Lett. (2005) 15: 1497-1500 [PMID:15713415]
ChEMBL	Agonist activity at human PPARalpha expressed in HEK293 cells cotransfected with PPREx4-TK-luc assessed as activation of luciferase activity measured after 48 hrs by transactivation assay	B	4.63	pEC50	23330	nM	EC50	Bioorg. Med. Chem. (2013) 21: 766-778 [PMID:23265844]
ChEMBL	Agonist activity at human PPARalpha assessed as luciferase activity by transactivation assay	F	4.63	pEC50	23330	nM	EC50	J. Med. Chem. (2009) 52: 6835-6850 [PMID:19807106]
ChEMBL	In vitro transcription activation on human peroxisome proliferator activated receptor-alpha (PPAR alpha)	B	4.81	pEC50	15500	nM	EC50	Bioorg. Med. Chem. Lett. (2004) 14: 3507-3511 [PMID:15177462]
ChEMBL	Agonist activity at human PPARalpha by transactivation assay	F	4.89	pEC50	13000	nM	EC50	Bioorg. Med. Chem. Lett. (2007) 17: 3198-3202 [PMID:17379517]
ChEMBL	Effective concentration for human peroxisome proliferator-activated receptor alpha	B	4.89	pEC50	13000	nM	EC50	Bioorg. Med. Chem. Lett. (2005) 15: 1497-1500 [PMID:15713415]
ChEMBL	In vitro transactivation of human Peroxisome proliferator activated receptor alpha (hPPARalpha)	B	4.9	pEC50	12600	nM	EC50	J. Med. Chem. (2003) 46: 4883-4894 [PMID:14584939]
ChEMBL	In vitro transactivation using receptor transactivation assay against hPPAR alpha	F	4.9	pEC50	12600	nM	EC50	J. Med. Chem. (2002) 45: 789-804 [PMID:11831892]
ChEMBL	Agonist activity at human PPARalpha in U2OS cells by transactivation assay	F	4.92	pEC50	12000	nM	EC50	Eur. J. Med. Chem. (2008) 43: 2428-2435 [PMID:18329751]
ChEMBL	Transactivation of human Gal4-fused PPARalpha LBD expressed in African green monkey COS7 cells after 24 hrs by luciferase reporter gene assay	B	5	pEC50	10000	nM	EC50	Eur J Med Chem (2017) 137: 310-326 [PMID:28609708]
ChEMBL	In vitro effective concentration against human peroxisome proliferator activated receptor alpha/Gal4 in transactivation assay	F	5	pEC50	>10000	nM	EC50	Bioorg. Med. Chem. Lett. (2005) 15: 4421-4426 [PMID:16137885]
ChEMBL	Agonist activity at GAL4-fused PPARalpha A454M mutant (unknown origin) expressed in human HepG2 cells by transactivation assay	B	5.15	pEC50	7100	nM	EC50	Eur. J. Med. Chem. (2013) 63: 321-332 [PMID:23502212]
GtoPdb	-	-	5.3	pEC50	-	-	-	J Med Chem (2000) 43: 527-50 [PMID:10691680]
ChEMBL	Agonist activity for Human PPAR alpha receptor in transcriptional activation assay	F	5.3	pEC50	5000	nM	EC50	J. Med. Chem. (2000) 43: 527-550 [PMID:10691680]
ChEMBL	Transactivation of human PPARalpha expressed in human HepG2 cells co-transfected with PPRE3-TK-Luc by luciferase reporter gene assay	B	5.32	pEC50	4800	nM	EC50	Bioorg. Med. Chem. Lett. (2011) 21: 3103-3109 [PMID:21450468]
ChEMBL	Agonist activity at PPARalpha expressed in human HepG2 cells assessed as induction of receptor transactivation by reporter gene assay relative to control	F	5.32	pEC50	4800	nM	EC50	Bioorg. Med. Chem. (2008) 16: 7117-7127 [PMID:18625559]
ChEMBL	Agonist activity at GAL4-fused PPARalpha (unknown origin) expressed in human HepG2 cells by transactivation assay	B	5.46	pEC50	3460	nM	EC50	Eur. J. Med. Chem. (2013) 63: 321-332 [PMID:23502212]
ChEMBL	Agonist activity at human PPARalpha expressed in African green monkey COS7 cells assessed as increase in receptor transcriptional activity by luciferase reporter gene assay relative to WY-14643	B	5.52	pEC50	3000	nM	EC50	J Nat Prod (2019) 82: 1802-1812 [PMID:31268307]
ChEMBL	Agonist activity at GAL4-DNA binding domain fused human PPARalpha ligand binding domain expressed in human HepG2 cells assessed as receptor transactivation incubated for 20 hrs by luciferase reporter gene assay	B	5.79	pEC50	1620	nM	EC50	Eur. J. Med. Chem. (2015) 90: 583-594 [PMID:25497132]
ChEMBL	Agonist activity at human PPARalpha ligand binding domain expressed in human Hep G2 cells co-transfected with Gal4-DBD by luciferase reporter gene assay	B	5.79	pEC50	1620	nM	EC50	J. Med. Chem. (2008) 51: 7768-7776 [PMID:19053776]
ChEMBL	Effective concentration against human PPARalpha expressed in HepG2 cells	B	5.8	pEC50	1600	nM	EC50	J. Med. Chem. (2005) 48: 5509-5519 [PMID:16107150]
ChEMBL	Agonist activity at human GAL4-fused PPARalpha ligand binding domain expressed in HepG2 cells after 20 hrs by luciferase reporter gene transactivation assay	B	5.8	pEC50	1600	nM	EC50	J. Med. Chem. (2012) 55: 37-54 [PMID:22081932]
ChEMBL	Agonist activity at GAL4-tagged human PPARalpha ligand binding domain expressed in human HepG2 cells assessed as receptor transactivation by luciferase reporter gene assay	B	5.8	pEC50	1600	nM	EC50	J. Med. Chem. (2009) 52: 6382-6393 [PMID:19775169]
ChEMBL	Transactivation of GAL4-fused human PPARalpa LBD expressed in human HepG2 cells after 20 hrs by luciferase reporter gene assay	B	5.8	pEC50	1600	nM	EC50	Eur J Med Chem (2017) 127: 379-397 [PMID:28076827]
ChEMBL	Agonist activity at GAL4-tagged human PPARalpha ligand binding domain expressed in HepG2 cells assessed as transactivation after 20 hrs by beta-galactosidase reporter gene assay	B	5.8	pEC50	1600	nM	EC50	Bioorg. Med. Chem. (2012) 20: 2141-2151 [PMID:22341573]
ChEMBL	Agonist activity at human GAL4-PPARalpha ligand binding domain expressed in human HepG2 cells by luciferase reporter gene assay	B	5.81	pEC50	1560	nM	EC50	Eur. J. Med. Chem. (2015) 89: 817-825 [PMID:25462281]
ChEMBL	Transactivation of GAL4-fused human PPARalpha ligand binding domain expressed in HepG2 cells after 20 hrs by luciferase reporter gene assay	B	5.81	pEC50	1560	nM	EC50	J. Med. Chem. (2013) 56: 60-72 [PMID:23171045]
ChEMBL	Transactivation of GAL4-tagged human PPARalpha LBD expressed in human HepG2 cells after 20 hrs by luciferase reporter gene assay	B	5.81	pEC50	1560	nM	EC50	J Med Chem (2018) 61: 8282-8298 [PMID:30199253]
ChEMBL	Activation of human PPARalpha by GST pull down assay	B	6.13	pEC50	740	nM	EC50	J. Med. Chem. (2007) 50: 389-393 [PMID:17228882]
ChEMBL	Agonist activity at PPARalpha assessed as receptor transactivation by reporter gene assay	F	6.15	pEC50	700	nM	EC50	J. Med. Chem. (2010) 53: 1076-1085 [PMID:20073471]
ChEMBL	Agonist activity at human PPARalpha expressed in MCF7 cells co-transfected CPTI DR1-type RE after 16 hrs by luciferase reporter gene assay	F	6.19	pEC50	650	nM	EC50	ACS Med. Chem. Lett. (2011) 2: 793-797 [PMID:24936232]
ChEMBL	Agonist activity at human PPARalpha expressed in MCF7 cells co-transfected CPTI DR1-type RE after 6 hrs by luciferase reporter gene assay	F	6.27	pEC50	542	nM	EC50	ACS Med. Chem. Lett. (2011) 2: 793-797 [PMID:24936232]
ChEMBL	Transactivation of human PPARalpha expressed in african green monkey CV1 cells by luciferase reporter gene assay	B	6.89	pEC50	130	nM	EC50	Bioorg. Med. Chem. Lett. (2012) 22: 4122-4126 [PMID:22579420]
Peroxisome proliferator-activated receptor-α/Peroxisome proliferator-activated receptor alpha in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2128] [GtoPdb: 593] [UniProtKB: P23204]
ChEMBL	Binding affinity towards peroxisome proliferator activated receptor alpha (murinePPAR alpha)	B	7	pKi	100	nM	Ki	Bioorg. Med. Chem. Lett. (2003) 13: 3185-3190 [PMID:12951090]
ChEMBL	Agonist activity for murine PPAR alpha receptor in transcriptional activation assay	F	6.2	pEC50	630	nM	EC50	J. Med. Chem. (2000) 43: 527-550 [PMID:10691680]
ChEMBL	Effective concentration against murine PPARalpha in transactivation assay	B	7.4	pEC50	40	nM	EC50	J. Med. Chem. (2005) 48: 5509-5519 [PMID:16107150]
ChEMBL	Agonist activity at mouse PPARalpha ligand binding domain expressed in human Hep G2 cells co-transfected with Gal4-DBD by luciferase reporter gene assay	B	7.4	pEC50	40	nM	EC50	J. Med. Chem. (2008) 51: 7768-7776 [PMID:19053776]
Peroxisome proliferator-activated receptor-α/Peroxisome proliferator-activated receptor alpha in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2129] [GtoPdb: 593] [UniProtKB: P37230]
ChEMBL	In vitro transactivation of rat Peroxisome proliferator activated receptor alpha	B	5.68	pEC50	2100	nM	EC50	J. Med. Chem. (2003) 46: 4883-4894 [PMID:14584939]
Peroxisome proliferator-activated receptor-β/δ/Peroxisome proliferator-activated receptor delta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3979] [GtoPdb: 594] [UniProtKB: Q03181]
ChEMBL	Activation of human Gal4-fused PPARdelta LBD transfected in HEK293T after 14 to 16 hrs by dual-glo luciferase reporter gene assay	B	4	pEC50	>100000	nM	EC50	J Med Chem (2019) 62: 2112-2126 [PMID:30702885]
ChEMBL	Agonist activity for Human PPAR delta receptor in transcriptional activation assay	F	4.46	pEC50	35000	nM	EC50	J. Med. Chem. (2000) 43: 527-550 [PMID:10691680]
ChEMBL	In vitro transcription activation on human peroxisome proliferator activated receptor-delta (PPAR delta)	B	5.84	pEC50	1460	nM	EC50	Bioorg. Med. Chem. Lett. (2004) 14: 3507-3511 [PMID:15177462]
Peroxisome proliferator-activated receptor-γ/Peroxisome proliferator-activated receptor gamma in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL235] [GtoPdb: 595] [UniProtKB: P37231]
ChEMBL	Binding affinity to recombinant PPARgamma (unknown origin) by isothermal titration calorimetry	B	5.87	pKd	1340	nM	Kd	J Med Chem (2019) 62: 2112-2126 [PMID:30702885]
ChEMBL	Binding affinity to PPARgamma (unknown origin) assessed as thermodynamic dissociation constant by SPR assay	B	5.89	pKd	1297.3	nM	Kd	Eur J Med Chem (2017) 127: 379-397 [PMID:28076827]
ChEMBL	Activation of human PPARgamma ligand binding domain expressed in COS7 cells by luciferase reporter gene assay	B	4.27	pIC50	53700	nM	IC50	Bioorg. Med. Chem. Lett. (2014) 24: 3757-3763 [PMID:25037914]
ChEMBL	Agonist activity for Human PPAR gamma receptor in transcriptional activation assay	F	4.22	pEC50	60000	nM	EC50	J. Med. Chem. (2000) 43: 527-550 [PMID:10691680]
ChEMBL	Activation of human Gal4-fused PPARgamma LBD transfected in HEK293T after 14 to 16 hrs by dual-glo luciferase reporter gene assay	B	4.27	pEC50	54000	nM	EC50	J Med Chem (2019) 62: 2112-2126 [PMID:30702885]
ChEMBL	Transactivation of human PPARgamma LBD expressed in african green monkey Cos7 cells co-transfected with fused GAL4-DBD after 14 hrs by Dual-Glo Luciferase reporter gene assay	B	4.27	pEC50	53700	nM	EC50	Bioorg. Med. Chem. Lett. (2010) 20: 2469-2473 [PMID:20307981]
ChEMBL	Agonist activity at human PPARgamma LBD expressed in COS7 cells after 12 hrs by Dual-Glo luciferase assay	B	4.27	pEC50	53700	nM	EC50	Bioorg. Med. Chem. Lett. (2014) 24: 4048-4052 [PMID:25022880]
ChEMBL	In vitro transactivation using receptor transactivation assay against hPPAR gamma	F	4.53	pEC50	29300	nM	EC50	J. Med. Chem. (2002) 45: 789-804 [PMID:11831892]
ChEMBL	In vitro transactivation of human Peroxisome proliferator activated receptor gamma	B	4.53	pEC50	29300	nM	EC50	J. Med. Chem. (2003) 46: 4883-4894 [PMID:14584939]
ChEMBL	Agonist activity at human PPARgamma by transactivation assay	F	4.54	pEC50	29000	nM	EC50	Bioorg. Med. Chem. Lett. (2007) 17: 3198-3202 [PMID:17379517]
ChEMBL	In vitro effective concentration against human peroxisome proliferator activated receptor gamma/Gal4 in cell-based transactivation assay	F	5	pEC50	>10000	nM	EC50	Bioorg. Med. Chem. Lett. (2005) 15: 4421-4426 [PMID:16137885]
ChEMBL	Agonist activity at human PPARgamma ligand binding domain expressed in human HepG2 cells co-transfected with Gal4 by luciferase reporter gene assay	B	5.8	pEC50	1600	nM	EC50	Bioorg. Med. Chem. (2008) 16: 9498-9510 [PMID:18835719]
Peroxisome proliferator-activated receptor-γ/Peroxisome proliferator-activated receptor gamma in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2459] [GtoPdb: 595] [UniProtKB: P37238]
ChEMBL	Agonist activity for murine PPAR gamma receptor in transcriptional activation assay	F	4.49	pEC50	32000	nM	EC50	J. Med. Chem. (2000) 43: 527-550 [PMID:10691680]
Retinoid X receptor-α/Retinoid X receptor alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2061] [GtoPdb: 610] [UniProtKB: P19793]
ChEMBL	Binding affinity to recombinant RXRalpha (unknown origin) by isothermal titration calorimetry	B	5.89	pKd	1280	nM	Kd	J Med Chem (2019) 62: 2112-2126 [PMID:30702885]
ChEMBL	Activation of human Gal4-fused RXRalpha LBD transfected in HEK293T after 14 to 16 hrs by dual-glo luciferase reporter gene assay	B	5.04	pEC50	9100	nM	EC50	J Med Chem (2019) 62: 2112-2126 [PMID:30702885]
Retinoid X receptor-β/Retinoid X receptor beta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1870] [GtoPdb: 611] [UniProtKB: P28702]
ChEMBL	Activation of human Gal4-fused RXRbeta LBD transfected in HEK293T after 14 to 16 hrs by dual-glo luciferase reporter gene assay	B	4.89	pEC50	13000	nM	EC50	J Med Chem (2019) 62: 2112-2126 [PMID:30702885]
Retinoid X receptor-γ/Retinoid X receptor gamma in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2004] [GtoPdb: 612] [UniProtKB: P48443]
ChEMBL	Activation of human Gal4-fused RXRgamma LBD transfected in HEK293T after 14 to 16 hrs by dual-glo luciferase reporter gene assay	B	4.51	pEC50	31000	nM	EC50	J Med Chem (2019) 62: 2112-2126 [PMID:30702885]

ChEMBL data shown on this page come from version 31:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, Cibrián-Uhalte E, Davies M, Dedman N, Karlsson A, Magariños MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]